关键词: 氯化汞, 草地贪夜蛾, 核型多角体病毒, 损伤, 突变

Abstract: 【Objective】In an experiment, sf9 cell of Autographa californica was used as an acceptor to inspect the effect of HgCl2 on its growth and development. 【Method】Evaluated the vigor of cells by repulsion of Trypan Blue dyeing method, detected the microkernel in the cells by HE dyeing method, amplified the DNA of HgCl2 treated AcMNPV by PCR, and analyzed the molecular mutation of the products after sequencing. 【Result】The results showed that the surface of sf9 cell became rough, and the division became slowly when treated with 4 μg·ml-1 HgCl2. When the dosage increased to 7 ?g·ml-1, the cell membrane broken. The cell integrity was damaged when the concentration increased to 9 ?g·ml-1. The microkernel in the cells was detected by using HE dyeing method. When treated with 9 ?g·ml-1 HgCl2, the microkernel was as high as 6.8%. Some cells found tri-nucleus and multi-nucleus, reflecting that the integrity of cell was damaged. After shortly treated with HgCl2 AcMNPV was inoculated to sf9 cell. The formation of polyhedra in treated sf9 cells was less than that in CK, and the abnormal polyhedra increased. DNA was extracted from AcMNPV treated with HgCl2, and inspected by PCR. The sequencing of amplification product showed that absent and change (such as G→C, T→C) were found in two bases of DNA. 【Conclusion】Certain dosage of HgCl2 can cause the damage and mutation of sf9 cell of Autographa californica and AcMNPV.

Key words: HgCl2, Autographa californica, NPV, Damage, Mutation