关键词: 姜, 烟草, GVDE反义基因, 转基因烟草, NPQ, Fv/Fm

Abstract: Abstract: In this experiment, we constructed an antisense VDE vector (VDE gene was cloned from ginger by our laboratory)to transform tobacco leaves for further research the characteristics of VDE. PCR amplification and PCR-Southern demonstrated that this antisense gene was successful integrated into tobacco genomes. The results shown that in transgenic tobacco and ck, NPQ was increased and Fv/Fm was decreased in treatments of different light intensity and in strong light with illuminating time prolonged, NPQ、Fv/Fm in transgenic tobacco were lower than CK, Our research also showed that the activities of APX、SOD and POD in transgenic tobacco were also lower than CK.