姜黄素通过SIRT1/FOXO1通路缓解玉米赤霉烯酮诱导的猪肾上皮细胞氧化损伤

doi:10.3864/j.issn.0578-1752.2023.05.015

中国农业科学 ›› 2023, Vol. 56 ›› Issue (5): 1007-1018.doi: 10.3864/j.issn.0578-1752.2023.05.015

• 研究简报 • 上一篇

姜黄素通过SIRT1/FOXO1通路缓解玉米赤霉烯酮诱导的猪肾上皮细胞氧化损伤

崔红杰¹^,²(), 卢春亭¹, 潘丽琴¹, 胡会¹, 钟佩云¹, 朱洁莹¹, 张凯照¹^,², 黄小红¹^,²()

¹ 福建农林大学动物科学学院，福州 350002
² 中西兽医结合与动物保健福建省高校重点实验室/福建省兽医中药与动物保健重点实验室，福建农林大学，福州 350002

收稿日期:2021-10-21 接受日期:2022-05-17 出版日期:2023-03-01 发布日期:2023-03-13
通信作者: 黄小红，Tel：13774550003；E-mail：984158392@qq.com
联系方式: 崔红杰，Tel：13696892917；E-mail：tsuisearcher@163.com。
基金资助:
福建省科技厅重大专项(2021NZ029008); 福建省自然科学基金(2021J01080); 福建省中青年教师教育科研项目(JAT210066); 横向课题项目(KH190363A)

Curcumin Alleviates Zearalenone-Induced Oxidative Damage in Porcine Renal Epithelial Cells via SIRT1/FOXO1 Pathway

CUI HongJie¹^,²(), LU ChunTing¹, PAN LiQin¹, HU Hui¹, ZHONG PeiYun¹, ZHU JieYing¹, ZHANG KaiZhao¹^,², HUANG XiaoHong¹^,²()

¹ College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002
² University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province/Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University, Fuzhou 350002

Received:2021-10-21 Accepted:2022-05-17 Published:2023-03-01 Online:2023-03-13

摘要/Abstract

摘要：

【目的】探究姜黄素（Cur）对玉米赤霉烯酮（ZEA）诱导猪肾上皮细胞（PK-15）氧化损伤的保护作用，并基于SIRT1/FOXO1信号通路阐明其作用机制，为姜黄素的兽医临床应用提供依据。【方法】试验分为5组：对照组、ZEA组（36.55 μg·mL^-1 ZEA）、Cur 6.25组（36.55 μg·mL^-1 ZEA+6.25 μmol·L^-1 Cur）、Cur 12.5组（36.55 μg·mL^-1 ZEA+12.5 μmol·L^-1 Cur）、Cur 25组（36.55 μg·mL^-1 ZEA +25 μmol·L^-1 Cur）；通过MTT法测定ZEA的半数抑制浓度和Cur对PK-15细胞的最大安全浓度；使用倒置显微镜观察PK-15细胞的形态变化；采用试剂盒检测细胞内活性氧（ROS）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）以及丙二醛（MDA）的水平；qRT-PCR检测细胞SIRT1、FOXO1、CAT、Mn-SOD的mRNA水平；Western Blot检测SIRT1、FOXO1和Acetyl-FOXO1蛋白的表达量。【结果】ZEA的IC₅₀为36.55 μg·mL^-1，Cur的最大安全浓度为25 μmol·L^-1；与对照组相比，ZEA极显著降低PK-15细胞活力（P<0.01），极显著提高ROS和MDA水平（P<0.01），极显著降低SOD、CAT活性（P<0.01）；与ZEA组对比，不同浓度的Cur（6.25、12.5、25 μmol·L^-1）显著提高PK-15细胞的存活率（P<0.05），改善细胞形态；极显著（P<0.01）降低ZEA诱导的ROS和MDA水平；极显著升高细胞内SOD和CAT活性（P<0.01）。qRT-PCR结果显示，与对照组相比，ZEA降低SIRT1 mRNA表达量，极显著升高FOXO1 mRNA表达量（P<0.01），升高Mn-SOD mRNA表达量，极显著降低CAT mRNA表达量（P<0.01）。与ZEA组对比，各Cur组不同程度的升高SIRT1和CAT的mRNA表达量，极显著的降低FOXO1的mRNA表达量（P<0.01）；极显著升高Mn-SOD的mRNA表达量（P<0.01）。Western Blot结果表明，与对照组对比，ZEA显著降低SIRT1蛋白表达（P<0.05），极显著升高Acetyl-FOXO1蛋白表达（P<0.01）；与ZEA组对比，各Cur组均极显著升高SIRT1蛋白表达（P<0.01），极显著降低Acetyl-FOXO1蛋白表达（P<0.01）。【结论】Cur可以上调SIRT1的表达，降低FOXO1的乙酰化水平，诱导抗氧化酶Mn-SOD和CAT的表达，从而清除ROS，降低MDA水平，减轻ZEA对PK-15细胞的氧化损伤作用。

关键词: 姜黄素, 玉米赤霉烯酮, 氧化应激, 沉默信息调节因子1 (SIRT1)

Abstract:

【Objective】 The purpose of this study was to investigate the protective effect of curcumin (Cur) on zearalenone (ZEA)-induced oxidative damage in porcine renal epithelial cells (PK-15), and to elucidate the protective mechanism based on SIRT1/FOXO1 signaling pathway. 【Method】 The experiment was divided into 5 groups: control group, ZEA group (36.55 μg·mL^-1 ZEA), Cur6.25 group (36.55 μg·mL^-1 ZEA+6.25 μmol·L^-1 Cur), Cur12.5 group (36.55 μg·mL^-1 ZEA+12.5 μmol·L^-1 Cur), and Cur25 group (36.55 μg·mL^-1 ZEA +25 μmol·L^-1 Cur). MTT assay was used to determine the half inhibitory concentration of ZEA and the maximum safe concentration of Cur on PK-15 cells. The morphological changes were observed by inverted microscope. The levels of intracellular reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) were detected by the reagent kits. Real-time quantitative PCR was used to detect the mRNA levels of SIRT1, FOXO1, CAT and Mn-SOD. The expression levels of SIRT1, FOXO1 and Acetyl-FOXO1 proteins were detected by Western Blot. 【Result】 The IC50 of ZEA was 36.55 μg·mL^-1, and the maximum safe concentration of Cur was 25 μmol·L^-1. Compared with the control group, ZEA significantly decreased the cell viability of PK-15 cells (P<0.01), significantly increased the levels of ROS and MDA (P<0.01), and significantly decreased the activities of SOD and CAT (P<0.01). Compared with ZEA group, the different concentrations of Cur (6.25, 12.5, 25 μmol·L^-1) significantly increased the cell viability of PK-15 cells (P<0.05) and improved the cell morphology. ROS and MDA levels induced by ZEA were also significantly reduced by Cur (P<0.01). Moreover, SOD and CAT activities in cells were significantly increased (P<0.01). qRT-PCR results showed that, compared with the control group, ZEA decreased SIRT1 mRNA expression, significantly increased FOXO1 mRNA expression (P<0.01), increased Mn-SOD mRNA expression, and significantly decreased CAT mRNA expression (P<0.01). Compared with ZEA group, mRNA expression levels of SIRT1 and CAT were increased in different degrees, FOXO1 mRNA expression levels were significantly decreased (P<0.01), and Mn-SOD mRNA expression levels were significantly increased (P<0.01) in all Cur groups. Western Blot results showed that ZEA significantly reduced SIRT1 protein expression (P<0.05), and significantly increased Acetyl-FOXO1 protein expression (P<0.01). Compared with ZEA group, SIRT1 protein expression was significantly increased (P<0.01), while Acetyl-FOXO1 protein expression was significantly decreased (P<0.01) in all Cur groups.【Conclusion】 Cur could up-regulate the expression of SIRT1, reduce the acetylation level of FOXO1, and induce the expression of antioxidant enzymes Mn-SOD and CAT, thereby eliminating ROS, reducing the level of MDA, and alleviating the oxidative damage of ZEA on PK-15 cells.

Key words: curcumin, zearalenone, oxidative stress, silencing information regulator 1 (SIRT1)

崔红杰, 卢春亭, 潘丽琴, 胡会, 钟佩云, 朱洁莹, 张凯照, 黄小红. 姜黄素通过SIRT1/FOXO1通路缓解玉米赤霉烯酮诱导的猪肾上皮细胞氧化损伤[J]. 中国农业科学, 2023, 56(5): 1007-1018.

CUI HongJie, LU ChunTing, PAN LiQin, HU Hui, ZHONG PeiYun, ZHU JieYing, ZHANG KaiZhao, HUANG XiaoHong. Curcumin Alleviates Zearalenone-Induced Oxidative Damage in Porcine Renal Epithelial Cells via SIRT1/FOXO1 Pathway[J]. Scientia Agricultura Sinica, 2023, 56(5): 1007-1018.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2023.05.015

https://www.chinaagrisci.com/CN/Y2023/V56/I5/1007

图/表 13

表1

表2

表3

图1

图2

图3

图4

图5

图6

图7

图8

图9

图10

参考文献 32

[1]	ZINEDINE A, SORIANO J M, MOLTÓ J C, MAÑES J. Review on the toxicity, occurrence, metabolism, detoxification, regulations and intake of Zearalenone: An oestrogenic mycotoxin. Food and Chemical Toxicology, 2007, 45(1): 1-18. doi:10.1016/j.fct.2006.07.030. doi: 10.1016/j.fct.2006.07.030 pmid: 17045381
[2]	ALSHANNAQ A, YU J H. Occurrence, toxicity, and analysis of major mycotoxins in food. International Journal of Environmental Research and Public Health, 2017, 14(6): 632. doi:10.3390/ijerph14060632. doi: 10.3390/ijerph14060632
[3]	GRUBER-DORNINGER C, JENKINS T, SCHATZMAYR G. Global mycotoxin occurrence in feed: A ten-year survey. Toxins, 2019, 11(7): 375. doi:10.3390/toxins11070375. doi: 10.3390/toxins11070375
[4]	王国强, 刘耀东, 段胜和. 2019年上半年饲料及饲料原料霉菌毒素污染及调查报告. 养猪, 2019(6): 17-20. doi:10.13257/j.cnki.21-1104/s.2019.06.008. doi: 10.13257/j.cnki.21-1104/s.2019.06.008
	WANG G Q, LIU Y D, DUAN S H. Mycotoxin contamination and investigation of feed and feed materials in the first half of 2019. Swine Production, 2019(6): 17-20. doi:10.13257/j.cnki.21-1104/s.2019.06. 008.(in Chinese) doi: 10.13257/j.cnki.21-1104/s.2019.06. 008
[5]	ROPEJKO K, TWARUŻEK M. Zearalenone and its metabolites- general overview, occurrence, and toxicity. Toxins, 2021, 13(1): 35. doi:10.3390/toxins13010035. doi: 10.3390/toxins13010035
[6]	KNUTSEN H K, ALEXANDER J, BARREGÅRD L, BIGNAMI M, BRÜSCHWEILER B, CECCATELLI S, COTTRILL B, DINOVI M, EDLER L, GRASL-KRAUPP B, et al. Risks for animal health related to the presence of Zearalenone and its modified forms in feed. EFSA Journal, 2017, 15(7): e04851. doi:10.2903/j.efsa.2017.4851. doi: 10.2903/j.efsa.2017.4851
[7]	姜淑贞, 孙华, 黄丽波, 杨在宾, 王淑静, 刘法孝, F. CHI. 不同水平玉米赤霉烯酮对断奶仔猪血清代谢产物和肝肾组织病理学影响. 中国农业科学, 2014, 47(18): 3708-3715. doi:10.3864/j.issn.0578-1752.2014.18.019. doi: 10.3864/j.issn.0578-1752.2014.18.019
	JIANG S Z, SUN H, HUANG L B, YANG Z B, WANG S J, LIU F X, F. CHI. Effects of Zearalenone contaminated diets on serum metabolite and histopathology of liver and kidney in weaned piglets. Scientia Agricultura Sinica, 2014, 47(18): 3708-3715. doi:10.3864/j.issn.0578-1752.2014.18.019.(in Chinese) doi: 10.3864/j.issn.0578-1752.2014.18.019
[8]	LIANG Z, REN Z H, GAO S, CHEN Y, YANG Y Y, YANG D, DENG J L, ZUO Z C, WANG Y, SHEN L H. Individual and combined effects of deoxynivalenol and Zearalenone on mouse kidney. Environmental Toxicology and Pharmacology, 2015, 40(3): 686-691. doi:10.1016/j.etap.2015.08.029. doi: 10.1016/j.etap.2015.08.029 pmid: 26407231
[9]	WANG N, LI P, PAN J W, WANG M Y, LONG M, ZANG J, YANG S H. Bacillus velezensis A2 fermentation exerts a protective effect on renal injury induced by Zearalenone in mice. Scientific Reports, 2018, 8(1): 13646. doi:10.1038/s41598-018-32006-z. doi: 10.1038/s41598-018-32006-z
[10]	ZHENG W L, WANG B J, LI X, WANG T, ZOU H, GU J H, YUAN Y, LIU X Z, BAI J F, BIAN J C, LIU Z P. Zearalenone promotes cell proliferation or causes cell death? Toxins, 2018, 10(5): 184. doi: 10.3390/toxins10050184. doi: 10.3390/toxins10050184
[11]	ZHENG W L, WANG B J, SI M X, ZOU H, SONG R L, GU J H, YUAN Y, LIU X Z, ZHU G Q, BAI J F, BIAN J C, LIU Z P. Zearalenone altered the cytoskeletal structure via ER stress- Autophagy-oxidative stress pathway in mouse TM4 sertoli cells. Scientific Reports, 2018, 8: 3320. Doi:10.1038/S41598-018-21567-8. doi: 10.1038/S41598-018-21567-8
[12]	YU J J, AUWERX J. The role of sirtuins in the control of metabolic homeostasis. Annals of the New York Academy of Sciences, 2009, 1173: E10-E19. doi:10.1111/j.1749-6632.2009.04952.x. doi: 10.1111/j.1749-6632.2009.04952.x
[13]	ZHANG X J, JIANG L S, LIU H M. Forkhead box protein O1: Functional diversity and post-translational modification, a new therapeutic target? Drug Design, Development and Therapy, 2021, 15: 1851-1860. doi:10.2147/DDDT.S305016. doi: 10.2147/DDDT.S305016 pmid: 33976536
[14]	LICZBIŃSKI P, MICHAŁOWICZ J, BUKOWSKA B. Molecular mechanism of curcumin action in signaling pathways: Review of the latest research. Phytotherapy Research, 2020, 34(8): 1992-2005. doi:10.1002/ptr.6663. doi: 10.1002/ptr.6663 pmid: 32141677
[15]	SIKORA-POLACZEK M, BIELAK-ZMIJEWSKA A, SIKORA E. Molecular and cellular mechanisms of curcumin action: Beneficial effect on organism. Postepy Biochemii, 2011, 57(1): 74-84.
[16]	BEN SALEM I, PROLA A, BOUSSABBEH M, GUILBERT A, BACHA H, ABID-ESSEFI S, LEMAIRE C. Crocin and Quercetin protect HCT116 and HEK293 cells from Zearalenone-induced apoptosis by reducing endoplasmic reticulum stress. Cell Stress & Chaperones, 2015, 20(6): 927-938. doi:10.1007/s12192-015-0613-0. doi: 10.1007/s12192-015-0613-0
[17]	CAO L, ZHAO J, MA L, CHEN J W, XU J R, RAHMAN S U, FENG S B, LI Y, WU J J, WANG X C. Lycopene attenuates Zearalenone- induced oxidative damage of piglet Sertoli cells through the nuclear factor erythroid-2 related factor 2 signaling pathway. Ecotoxicology and Environmental Safety, 2021, 225: 112737. doi:10.1016/j.ecoenv.2021.112737. doi: 10.1016/j.ecoenv.2021.112737
[18]	LECOMTE S, LELONG M, BOURGINE G, EFSTATHIOU T, SALIGAUT C, PAKDEL F. Assessment of the potential activity of major dietary compounds as selective estrogen receptor modulators in two distinct cell models for proliferation and differentiation. Toxicology and Applied Pharmacology, 2017, 325: 61-70. doi:10.1016/j.taap.2017.04.005. doi: S0041-008X(17)30154-0 pmid: 28396216
[19]	KHOSROKHAVAR R, RAHIMIFARD N, SHOEIBI S, HAMEDANI M P, HOSSEINI M J. Effects of zearalenone and alpha-zearalenol in comparison with raloxifene on t47d cells. Toxicology Mechanisms and Methods, 2009, 19(3): 246-250. doi:10.1080/15376510802455347. doi: 10.1080/15376510802455347 pmid: 19730705
[20]	ZONG S H, ZENG G F, FANG Y, PENG J Z, ZOU B, GAO T H, ZHAO J M. The effects of α-Zearalanol on the proliferation of bone-marrow-derived mesenchymal stem cells and their differentiation into osteoblasts. Journal of Bone and Mineral Metabolism, 2016, 34(2): 151-160. doi:10.1007/s00774-015-0659- 1. doi: 10.1007/s00774-015-0659-1 pmid: 25944420
[21]	CORTINOVIS C, CALONI F, SCHREIBER N B, SPICER L J. Effects of fumonisin B1 alone and combined with deoxynivalenol or Zearalenone on porcine granulosa cell proliferation and steroid production. Theriogenology, 2014, 81(8): 1042-1049. doi:10.1016/j.theriogenology.2014.01.027. doi: 10.1016/j.theriogenology.2014.01.027 pmid: 24576714
[22]	ZHENG W L, PAN S Y, WANG G G, WANG Y J, LIU Q, GU J H, YUAN Y, LIU X Z, LIU Z P, BIAN J C. Zearalenone impairs the male reproductive system functions via inducing structural and functional alterations of Sertoli cells. Environmental Toxicology and Pharmacology, 2016, 42: 146-155. doi:10.1016/j.etap.2016.01.013. doi: 10.1016/j.etap.2016.01.013 pmid: 26851377
[23]	SANG Y Q, LI W Z, ZHANG G Y. The protective effect of resveratrol against cytotoxicity induced by mycotoxin, Zearalenone. Food & Function, 2016, 7(9): 3703-3715. doi:10.1039/c6fo00191b. doi: 10.1039/c6fo00191b
[24]	QIN X S, CAO M J, LAI F N, YANG F, GE W, ZHANG X F, CHENG S F, SUN X F, QIN G Q, SHEN W, LI L. Oxidative stress induced by Zearalenone in porcine granulosa cells and its rescue by curcumin in vitro. PLoS ONE, 2015, 10(6): e0127551. doi:10.1371/journal.pone.0127551. doi: 10.1371/journal.pone.0127551
[25]	WANG J J, LI M M, ZHANG W, GU A X, DONG J W, LI J P, SHAN A S. Protective effect of N-acetylcysteine against oxidative stress induced by Zearalenone via mitochondrial apoptosis pathway in SIEC02 cells. Toxins, 2018, 10(10): 407. doi:10.3390/toxins10100407. doi: 10.3390/toxins10100407
[26]	ZHENG S Z, FU Y M, CHEN A P. De novo synthesis of glutathione is a prerequisite for curcumin to inhibit hepatic stellate cell (HSC) activation. Free Radical Biology and Medicine, 2007, 43(3): 444-453. doi:10.1016/j.freeradbiomed.2007.04.016. doi: 10.1016/j.freeradbiomed.2007.04.016 pmid: 17602960
[27]	SAHIN K, ORHAN C, TUZCU Z, TUZCU M, SAHIN N. Curcumin ameloriates heat stress via inhibition of oxidative stress and modulation of Nrf2/HO-1 pathway in quail. Food and Chemical Toxicology, 2012, 50(11): 4035-4041. doi:10.1016/j.fct.2012.08.029. doi: 10.1016/j.fct.2012.08.029 pmid: 22939939
[28]	张婧菲. 姜黄素对动物线粒体氧化损伤的保护作用及其抗氧化机制研究[D]. 南京: 南京农业大学, 2015.
	ZHANG J F. The protective effects of curcumin on mitochondrial oxidant damages in animals and the potential antioxidant mechanism[D]. Nanjing: Nanjing Agricultural University, 2015.(in Chinese)
[29]	李光辉, 钟秀伶, Aamir Nawab, 赵一, 效梅, 刘文超, 兰瑞霞, 吴江, 安立龙. 姜黄素对罗曼蛋鸡血液和组织抗氧化性能的影响. 安徽农业科学, 2018, 46(21): 96-99. doi:10.3969/j.issn.0517-6611.2018.21.027. doi: 10.3969/j.issn.0517-6611.2018.21.027
	LI G H, ZHONG X L, NAWAB A, ZHAO Y, XIAO M, LIU W C, LAN R X, WU J, AN L L. Effects of curcumin on the antioxidant properties in the blood and tissues of Roman laying hens. Journal of Anhui Agricultural Sciences, 2018, 46(21): 96-99. doi:10.3969/j.issn.0517-6611.2018.21.027.(in Chinese) doi: 10.3969/j.issn.0517-6611.2018.21.027
[30]	姜春晖, 孙旭东, 唐燕, 罗胜缤, 徐闯, 陈媛媛. 姜黄素通过Nrf2信号通路对H₂O₂诱导奶牛乳腺上皮细胞氧化应激的缓解. 中国农业科学, 2021, 54(8): 1787-1794. doi:10.3864/j.issn.0578-1752.2021.08.017. doi: 10.3864/j.issn.0578-1752.2021.08.017
	JIANG C H, SUN X D, TANG Y, LUO S B, XU C, CHEN Y Y. Curcumin alleviates H₂O₂-induced oxidative stress in bovine mammary epithelial cells via the Nrf2 signaling pathway. Scientia Agricultura Sinica, 2021, 54(8): 1787-1794. doi:10.3864/j.issn.0578-1752.2021.08.017.(in Chinese) doi: 10.3864/j.issn.0578-1752.2021.08.017
[31]	CARAFA V, ROTILI D, FORGIONE M, CUOMO F, SERRETIELLO E, HAILU G S, JARHO E, LAHTELA-KAKKONEN M, MAI A, ALTUCCI L. Sirtuin functions and modulation: from chemistry to the clinic. Clinical Epigenetics, 2016, 8: 61. doi:10.1186/s13148-016-0224-3. doi: 10.1186/s13148-016-0224-3 pmid: 27226812
[32]	CHEN J Y, LU Y, TIAN M Y, HUANG Q R. Molecular mechanisms of FOXO1 in adipocyte differentiation. Journal of Molecular Endocrinology, 2019, 62(3): R239-R253. doi:10.1530/JME-18-0178. doi: 10.1530/JME-18-0178

基因 Target gene	登录号 Accession No.	引物序列Primer sequence (5′→3′)	片段长度 Product length (bp)
SIRT1	NM_001145750.2	F:CGGCAGGAGAAGGAAACAATGGG R:TCGTCGTCGTCGTCGTAGAAGTC	88
FOXO1	NM_214014.3	F:TGTCCTACGCCGACCTCATCAC R:GCACGCTCTTGACCATCCACTC	96
CAT	NM_214301.2	F:CGCCTATTTGCCTATCCTGACACTC R:GCACGGAAGGGACAGTTCACAG	83
Mn-SOD	NM_214127.2	F:TTTCTGGACAAATCTGAGCCCTAACG R:CGACGGATACAGCGGTCAACTTC	122
β-Actin	NC_010445.4	F:CAAGGACCTCTACGCCAACAC R: TGGAGGCGCGATGATCTT	130

试剂 Reagent	体积 Volume (μL)
TB Green Premix Ex Taq П	10
上游引物 Upstream Primer	0.8
下游引物 Travelling Primer	0.8
Rox Reference Dye П (50×)	0.4
DNA模板 DNA template	2
灭菌水 Sterile water	6
总体系 Overall system	20

姜黄素通过SIRT1/FOXO1通路缓解玉米赤霉烯酮诱导的猪肾上皮细胞氧化损伤

Curcumin Alleviates Zearalenone-Induced Oxidative Damage in Porcine Renal Epithelial Cells via SIRT1/FOXO1 Pathway

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 13

参考文献 32

相关文章 15

Metrics

本文评价

推荐阅读 0

步骤 Step	循环数 Number of cycles	温度Temperature (℃)	时间Time (s)
预变性 Pre-denaturation	1	95	30
PCR反应PCR reaction	40	95	5
PCR反应PCR reaction	40	60	34

[1]	郗蒙雪, 沈丹, 石一凡, 李春梅. TBHQ对鸡舍PM_2.5诱导鸡胚肺组织细胞焦亡、坏死和炎症损伤的影响[J]. 中国农业科学, 2023, 56(4): 779-787.
[2]	陶文静, 张子婷, 刘媛, 宋丹, 李向臣. N-乙酰半胱氨酸对双酚A诱导的猪肾细胞凋亡和炎症反应的抑制作用[J]. 中国农业科学, 2023, 56(3): 549-558.
[3]	杨昌沛,王乃秀,汪锴,黄子晴,林海烂,张莉,张晨,冯露秋,甘玲. 外源性γ-氨基丁酸抵抗仔猪氧化应激的效果及机制[J]. 中国农业科学, 2022, 55(17): 3437-3449.
[4]	姜春晖,孙旭东,唐燕,罗胜缤,徐闯,陈媛媛. 姜黄素通过Nrf2信号通路对H₂O₂诱导奶牛乳腺上皮细胞氧化应激的缓解[J]. 中国农业科学, 2021, 54(8): 1787-1794.
[5]	黄丽波,王金全,高文博,陈红菊,侯衍猛,袁学军,王春阳. 玉米赤霉烯酮吸附剂对后备母猪子宫中LC3、PCNA分布和表达的影响[J]. 中国农业科学, 2021, 54(18): 4008-4017.
[6]	吕楚阳,邓平川,张晓丽,孙钰超,梁五生,胡东维. 低温诱导稻曲病菌菌核形成的转录组学分析[J]. 中国农业科学, 2020, 53(22): 4571-4583.
[7]	李函彤,甲承立,张书文,芦晶,逄晓阳,刘鹭,吕加平. 硫对酵母生物富铬过程中铬胁迫的缓解作用[J]. 中国农业科学, 2019, 52(6): 1078-1089.
[8]	周敏，周雪梅，杨立杰，黄丽波，冯蕾，邵明慧，杨晨，杨维仁，杨在宾，姜淑贞 . 玉米赤霉烯酮对断奶小母猪子宫形态学及热应激蛋白70分布和表达的影响[J]. 中国农业科学, 2018, 51(4): 778-788.
[9]	姜淑贞，孙华，黄丽波，杨在宾，王淑静，刘法孝，F. Chi. 不同水平玉米赤霉烯酮对断奶仔猪血清代谢产物和肝肾组织病理学影响[J]. 中国农业科学, 2014, 47(18): 3708-3715.
[10]	汤琴, 邓媛元, 张瑞芬, 张雁, 张名位, 魏振承, 唐小俊, 刘磊, 遆慧慧, 马永轩. 苦瓜水提物对拘束应激小鼠脂代谢紊乱的改善作用[J]. 中国农业科学, 2014, 47(16): 3300-3307.
[11]	赵娇, 周招洪, 梁小芳, 毛湘冰, 陈代文, 余冰. 葡萄籽原花青素及维生素E对氧化应激仔猪生长性能、血清氧化还原状态和肝脏氧化损伤的影响[J]. 中国农业科学, 2013, 46(19): 4157-4164.
[12]	罗金香, 丁伟, 张永强, 杨振国, 李阳, 丁丽娟. 双去甲氧基姜黄素及其N-甲基吡唑衍生物对朱砂叶螨生物活性和多种酶活性的影响[J]. 中国农业科学, 2013, 46(13): 2833-2844.
[13]	姜淑贞, 杨维仁, 杨在宾, 王淑静, 刘法孝, Chi F. 玉米赤霉烯酮污染日粮添加改性蒙脱石对断奶仔猪免疫指标的影响[J]. 中国农业科学, 2012, 45(16): 3382-3390.
[14]	崔志文, 黄琴, 黄怡, 吴红照, 文静, 李卫芬. 鼠李糖乳酸杆菌对Caco-2细胞抗氧化功能的影响[J]. 中国农业科学, 2011, 44(23): 4926-4932.
[15]	汪纪仓, 刘学忠, 袁燕, 裔传卉, 卞建春, 刘宗平. 氧化应激在镉致大鼠肝细胞凋亡中的作用[J]. 中国农业科学, 2011, 44(18): 3895-3902.