中国农业科学

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最新录用:不同控释氮素比例对夏玉米叶片衰老和籽粒灌浆特性的影响

于浩东,初振宇,汪顺源,郭艳青,任佰朝*,张吉旺*
  

  1. 山东农业大学农学院/作物生物学国家重点试验室,山东泰安 271018
  • 发布日期:2023-05-20

Effects of Different Controlled Nitrogen Ratios on Leaf Senescence and Grain Filling Characteristics of Summer Maize

YU HaoDong, CHU ZhenYu, WANG ShunYuan, GUO YanQing, REN BaiZhao*, ZHANG JiWang*  #br#   

  1. College of Agronomy, Shandong Agricultural University/State Key Laboratory of Crop Biology, Tai’an 271018, Shandong
  • Online:2023-05-20

摘要: 【目的】及时有效地供应氮素可以延缓叶片衰老,改善籽粒灌浆特性,提高玉米产量。研究不同控氮比例控释肥对夏玉米衰老特性、籽粒灌浆特性及产量的影响,探究适合黄淮海地区夏玉米生产的适宜控氮比例,为黄淮海地区夏玉米种肥同播技术提供科学依据。【方法】20192021年,选用中早熟品种登海518DH518)和中晚熟品种登海605DH605)为试验材料。设置10%T1)、20%T2)、30%T3)、40%T4)、50%T55个控氮比例处理,以普通尿素(CK)处理为对照,探讨不同控氮比控释肥对夏玉米衰老特性和籽粒灌浆特性的影响。【结果】不同控氮比控释肥可以显著提高夏玉米产量,其中,30%控氮比的控释肥效果最佳。与普通尿素相比,不同控氮比控释肥均可显著增加叶面积指数(LAI)和叶片SPAD值,提高叶片抗氧化酶活性,改善籽粒灌浆特性。控氮比为30%的控释肥较其他处理显著提高夏玉米生育后期叶片中的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT活性,降低叶片丙二醛(MDA)积累量,延缓叶片衰老;显著提高灌浆速率最大时的生长量(Wmax)、最大灌浆速率(Gmax)和灌浆活跃期(P)天数,提高成熟期籽粒脱水速率,改善籽粒灌浆特性;从而增加籽粒干物质积累,提高籽粒千粒重,进而提高产量。相关性分析表明,产量与Wmax、Gmax显著正相关关系。Wmax 和Gmax与SODPOD活性呈显著正相关关系,而与MDA含量呈显著负相关关系;籽粒脱水速率和籽粒灌浆速率与叶片SODCAT活性呈极显著正相关,与叶片MDA含量呈极显著负相关;籽粒含水量与叶片SODCAT活性呈极显著负相关,与叶片MDA含量呈极显著正相关。因此,施用控释肥可以通过提高叶片抗氧化酶活性,显著改善籽粒灌浆特性,从而提高夏玉米产量。两品种叶片衰老特性、籽粒灌浆特性和产量等对于不同比例控释肥的响应基本一致,均为控氮比30%的控释肥效果最佳。【结论】控释氮肥可显著提高叶片抗氧化酶活性,延缓叶片衰老,改善籽粒灌浆特性,从而提高玉米产量,其中,控释氮素30%增产效果最好。


关键词: 夏玉米, 控释肥, 控氮比, 籽粒灌浆特性, 衰老特性, 产量

Abstract: ObjectiveSupplying nitrogen timely and effectively could delay leaf senescence and improve grain filling characteristics of maize. The effects of controlled-release fertilizers with different controlled nitrogen ratio on leaf senescence, grain filling and yield of summer maize were studied to explore the appropriate controlled nitrogen ratio for summer maize in Huang-Huai-Hai region. MethodThis study was conducted in 2019-2021 cropping seasons, using the middle-early maturing hybrid Denghai 518 (DH518) and the middle-late maturing hybrid Denghai 605 (DH605) as experimental materials. Five controlled nitrogen ratios: 10% (T1), 20% (T2), 30% (T3), 40% (T4) and 50% (T5) were set for studying the effects of controlled-release fertilizers on leaf senescence, grain filling characteristics, and their inter-relationship of summer maize, using urea fertilizer treatment as control (CK). ResultThe results showed that using controlled release fertilizers could significantly increase the yield of summer maize. Among these, the yield increased most significantly in T3 treatment. Compared with CK, using controlled release fertilizers significantly increased the leaf area index (LAI), SPAD value, and the antioxidant enzymes activities, thus improving the grain filling characteristics. Among these, T3 treatment increased the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in summer maize leaves at the late growth stage, reducing the accumulation of malondialdehyde (MDA), thus delaying leaf senescence most significantly. In addition, the maximum growth (Wmax), grain filling rate (Gmax), days of active filling period (P), and grain dehydration rate were increased mostly in T3 treatment, accordingly the 1000-kernel weight and grain yield of summer maize were increased mostly. Correlation analysis showed that the yield was positively correlated with Wmax and Gmax; Wmax and Gmax was positively correlated with the activities of SOD and POD, but negatively correlated with MDA content. Grain dehydration rate and grain filling rate were positively correlated with the activities of SOD and CAT, but negatively correlated with MDA content. The water content of kernels was negatively correlated with the activities of SOD and CAT, and positively correlated with MDA content. Therefore, using controlled release fertilizers, especially T3 treatment, effectively optimized the grain filling characteristics by increasing the antioxidant enzyme activities, thus increasing the grain yield of summer maize.ConclusionUsing controlled-release fertilizer could effectively increase the activities of leaf antioxidant enzymes, thus delaying leaf senescence, improving grain filling characteristics and increasing grain yield of summer maize. Among these, T3 treatment increased the grain yield most significantly.


Key words: summer maize, controlled release fertilizer, controlled nitrogen ratios, grain filling characteristics, aging characteristics, yield