绵羊ADIPOQ多态性与生长性状的关联分析

doi:10.3864/j.issn.0578-1752.2022.11.013

摘要/Abstract

摘要：

【目的】探讨ADIPOQ遗传变异对绵羊生长性状的影响,找到与宁夏优质肉羊培育中生长性状相关的分子遗传标记,为宁夏优质肉羊的分子辅助育种的研究提供依据。【方法】首先利用液相捕获测序技术获得ADIPOQ在杜泊羊、滩羊和小尾寒羊中的变异位点,同时采集383只3个品种的不同杂交后代群体耳组织,采用飞行质谱技术对确定的SNPs位点进行基因分型检测,并使用Haploview软件对多态性位点进行连锁不平衡分析和构建单倍型,与绵羊初生和3月龄的生长性状进行关联分析。【结果】共筛选到7个SNPs位点,且7个位点在杂交后代群体中均表现出多态性,SNP1—SNP7位点的优势基因型分别为CC、GG、GG、CT、AG、GG和AA,优势等位基因分别为C、G、G、C、G、G和A。X ²检验发现,所有位点均处于Hardy-Weinberg平衡状态（除SNP7位点在所有个体中偏离了平衡）。SNP1（F₂代除外）、SNP4、SNP5和SNP6位点在各杂交后代及所有个体中均处于中度多态（0.25≤PIC<0.50）,SNP2和SNP3在F₂代及SNP7在H1代群体中均处于中度多态（0.25≤PIC<0.50）,而在其他群体中处于低度多态（PIC<0.25）。连锁不平衡分析发现,SNP2-SNP3和SNP5-SNP6形成了两处强连锁,均构建出3种单倍型,组合后分别形成4和6种基因型,其中优势基因型分别为H1H1和H4H6。将SNP2-SNP3和SNP5-SNP6形成的单倍型再组合后产生13种基因型,其优势基因型为H1H1H4H6。单个位点关联分析表明：SNP1位点在F₁代群体中,GG基因型的初生胸围显著高于CG基因型（P<0.05）,在H2代群体中,CC基因型的初生体斜长显著高于CG基因型（P<0.05）。SNP2位点在H₁代群体中,CC基因型的初生体高显著低于CG和GG基因型（P<0.05）。SNP3位点在F₁代群体中,AG基因型的3月龄体重显著高于GG基因型（P<0.05）,在H₁代群体中,AA基因型的初生体高显著低于AG和GG基因型（P<0.05）。SNP4位点在F₁代群体中,CC基因型的3月龄体重显著高于CT和TT基因型（P<0.05）,在F₂代群体中,CT基因型的初生体重和初生胸围显著高于TT基因型（P<0.05）,在H₁代群体中,TT基因型的初生体重显著低于CC和CT基因型（P<0.05）,在H₂代群体中,TT基因型的初生体高和初生体斜长显著高于CT基因型（P<0.05）。SNP5位点在F₂代群体中,AA基因型的初生体高和初生体斜长显著高于GG基因型（P<0.05）。SNP6位点的不同基因型在F₂代、H₁代和H₂代的初生体重、体高、体斜长、3月龄体高和胸围上均有不同程度的显著差异（P<0.05）。SNP7位点在H2代群体中,AA基因型的初生体斜长显著高于GA基因型（P<0.05）。联合所有群体进行分析时发现,SNP2位点CG基因型的初生体重和体斜长显著高于GG基因型（P<0.05）,SNP4位点TT基因型的初生体重显著低于CC和CT基因型（P<0.05）,SNP6位点AA基因型的初生体重显著高于GG和GA基因型（P<0.05）、初生胸围显著高于GG基因型（P<0.05）,其他5个位点的基因型在生长性状上均无显著差异（P>0.05）。单倍型组合关联分析发现：H2H3基因型的初生体重和初生体斜长显著高于其他基因型（P<0.05）;而SNP5-SNP6单倍型组合中,H5H5基因型的初生体重显著高于H5H6和H6H6（P<0.05）、初生体高显著高于H5H6（P<0.05）、初生胸围显著高于H6H6（P<0.05）,H4H4基因型的3月龄体重显著高于H5H5基因型（P<0.05）、3月龄体高和体斜长显著高于H4H6基因型（P<0.05）、3月龄胸围显著高于H4H5、H5H6和H5H5基因型（P<0.05）。SNP2-SNP3和SNP5-SNP6形成的单倍型再组合后,H2H3H4H4基因型的初生体重、体高、体斜长和胸围最高,与其他基因型有不同程度的差异,H1H1H4H6的3月龄体斜长显著低于H1H2H4H4和H2H2H4H4基因型（P<0.05）。【结论】 ADIPOQ的遗传变异对绵羊的生长性状具有不同程度的影响,研究中的7个SNPs位点可以作为宁夏优质肉羊选育中生长性状的潜在分子标记。

关键词: 绵羊, ADIPOQ, 多态性, 生长性状, 单倍型

Abstract:

【Objective】 The aim of the study was to explore the effects of genetic variation of ADIPOQ on growth traits of sheep, and to find the molecular genetic markers related to growth traits in Ningxia high-quality mutton sheep breeding, so as to achieve the purpose of molecular assisted breeding. 【Method】 The mutation sites of ADIPOQ in Dupo sheep, Tan sheep and Small-Tailed Han sheep were obtained by Allegro Targeted Genotyping. At the same time, the ear tissues of 383 different hybrid progenies of three breeds were collected. The SNPs were genotyped by Sequenom Mass ARRAY®SNP. Haploview was used to analyze linkage disequilibrium and construct haplotypes of the polymorphic loci, as well as association analysis between the SNPs in ADIPOQ with growth traits of newborn and 3-month-old sheep.【Result】 A total of 7 SNPs were screened, and 7 SNPs showed polymorphism in the hybrid population. The dominant genotypes of SNP1-SNP7 were CC, GG, GG, CT, AG, GG and AA, and the dominant alleles were C, G, G, C, G, G and A. X² test showed that all loci were in Hardy Weinberg equilibrium (except SNP7 site deviated from the equilibrium in all individuals). SNP1 (except F₂), SNP4, SNP5 and SNP6 were moderately polymorphic (0.25≤PIC<0.50) in all hybrids and all individuals, while SNP2 and SNP3 were moderately polymorphic (0.25≤PIC<0.50) in F₂ and SNP7 were moderately polymorphic (0.25≤PIC<0.50) in H₁, and were low polymorphic (PIC<0.25) in other populations. The results of linkage imbalance analysis showed that SNP2-SNP3 and SNP5-SNP6 formed two strong linkage, each of which constructed three haplotypes, and formed 4 and 6 genotypes after combination, respectively, among which the dominant genotype was H1H1 and H4H6. 13 genotypes were produced after the haplotypes formed by SNP2-SNP3 and SNP5-SNP6 were recombined, and the dominant genotype was H1H1H4H6. Single SNP correlation analysis revealed that: SNP1 site in F₁ population, the primary chest of GG genotype was significantly higher than that of CG genotype (P<0.05); in H₂ population, the primary body length of CC genotype was significantly higher than that of GG genotype (P<0.05). SNP2 site in H₁ population, the primary body height of CC genotype was significantly lower than that of CG and GG genotypes (P<0.05). SNP3 site in F₁ population, the three months body weight of AG genotype was significantly higher than that of GG genotype; in H₁ population, the primary body height of AA genotype was significantly lower than AG and GG genotypes (P<0.05). SNP4 site in F₁ population, the three months weight of CC genotype was significantly higher than that of CT and TT genotypes (P<0.05); in F₂ population, the primary weight and primary chest of CT genotype were significantly higher than that of TT genotype (P<0.05), which of TT genotype was significantly lower than that of CC and CT genotype (P<0.05) in H₁ population; in H₂ population, the primary body height and primary body length of TT genotype were significantly higher than that of CT genotype (P<0.05). SNP5 site in F₂ population, AA genotype had significantly higher primary body height and primary body length than GG genotype (P<0.05). There were significant differences in primary weight, body height, body length, three months body height and chest among different genotypes of SNP6 (P<0.05). SNP7 site in H₂ population, AA genotype had significantly higher primary body length than GA genotype (P<0.05). When combined with all groups, it was found that: the primary weight and body length of CG genotype at SNP2 were significantly higher than that of GG genotype (P<0.05); the primary weight of TT genotype at SNP4 was significantly lower than CC and CT genotypes (P<0.05); the primary weight of AA genotype at SNP6 was significantly higher than GG and GA genotype (P<0.05); and the primary chest was significantly higher than GG genotypes (P<0.05). There was no significant difference in growth traits among the other five genotypes (P>0.05). The results of haplotype association analysis showed that the primary weight and primary body length of H2H3 genotype were significantly higher than those of other genotypes (P<0.05), while the primary weight of H5H5 genotype was significantly higher than that of H5H6 and H6H6 (P<0.05), the primary body height was significantly higher than that of H5H6 (P<0.05), and the primary chest was significantly higher than that of H6H6 (P<0.05); the three months weight of H4H4 genotype was significantly higher than that of H5H5 genotype (P<0.05), the body weight and body length at three months were significantly higher than those of H4H6 genotype (P<0.05), and the chest of three months was significantly higher than that of H4H5, H5H6 and H5H5 genotype (P<0.05). After SNP2-SNP3 and SNP5-SNP6 haplotypes were recombined, the primary weight, body height, body length and chest of H2H3H4H4 genotype were the highest, which were different from other genotypes, and the body length at three months of H1H1H4H6 genotype were significantly lower than that of H1H2H4H4 and H2H2H4H4 genotypes (P<0.05).【Conclusion】 The results showed that the different SNPs and combined genotypes in ADIPOQ had effect on sheep different growth traits, the seven SNPs in this study could be used as potential molecular markers for growth traits in Ningxia high quality mutton sheep breeding.

Key words: sheep, ADIPOQ, polymorphisms, growth traits, haplotype

梁鹏,张天闻,孟科,邵顺成,邹诗凡,荣轩,强浩,冯登侦. 绵羊ADIPOQ多态性与生长性状的关联分析[J]. 中国农业科学, 2022, 55(11): 2239-2256.

LIANG Peng,ZHANG TianWen,MENG Ke,SHAO ShunCheng,ZOU ShiFan,RONG Xuan,QIANG Hao,FENG DengZhen. Association Analysis of the ADIPOQ Variation with Sheep Growth Traits[J]. Scientia Agricultura Sinica, 2022, 55(11): 2239-2256.

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链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2022.11.013

https://www.chinaagrisci.com/CN/Y2022/V55/I11/2239

图/表 17

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表4

ADIPOQ各SNPs位点的群体遗传多样性分析"

SNPs	群体 Groups	数量 Number	基因型频率 Genotype frequency			基因频率 Allele frequency		纯合度 Ho	杂合度 He	有效等位基因数Ne	多态信息含量PIC	X²(P值)
SNPs	群体 Groups	数量 Number	CC	CG	GG	C	G	纯合度 Ho	杂合度 He	有效等位基因数Ne	多态信息含量PIC	X²(P值)
SNP1	F₁	116	0.63(73)	0.32(37)	0.05(6)	0.79	0.21	0.67	0.33	1.50	0.28	0.21(0.90)
	F₂	144	0.73(105)	0.26(37)	0.01(2)	0.86	0.14	0.76	0.24	1.32	0.21	0.39(0.82)
	H₁	99	0.63(62)	0.37(37)	0.00(0)	0.81	0.19	0.70	0.30	1.44	0.26	5.23(0.07)
	H₂	23	0.70(16)	0.26(6)	0.04(1)	0.83	0.17	0.71	0.29	1.40	0.25	0.20(0.91)
	所有个体 All individuals	382	0.67(256)	0.31(117)	0.02(9)	0.82	0.18	0.71	0.29	1.41	0.25	1.06(0.59)
SNP2			CC	CG	GG	C	G
	F₁	113	0.02(2)	0.19(21)	0.79(90)	0.11	0.89	0.80	0.20	1.24	0.18	0.35(0.84)
	F₂	137	0.06(8)	0.35(48)	0.59(81)	0.23	0.77	0.64	0.36	1.56	0.29	0.06(0.97)
	H₁	95	0.02(2)	0.25(24)	0.73(69)	0.15	0.85	0.75	0.25	1.34	0.22	0.00(1.00)
	H₂	23	0.04(1)	0.09(2)	0.87(20)	0.09	0.91	0.84	0.16	1.19	0.15	4.71(0.10)
	所有个体 All individuals	368	0.04(13)	0.26(95)	0.70(260)	0.16	0.84	0.73	0.27	1.38	0.24	1.34(0.51)
SNP3			AA	AG	GG	A	G
	F₁	113	0.02(2)	0.18(21)	0.80(90)	0.11	0.89	0.80	0.20	1.24	0.18	0.35(0.84)
	F₂	142	0.06(8)	0.35(50)	0.59(84)	0.23	0.77	0.64	0.36	1.55	0.29	0.02(0.99)
	H₁	98	0.02(2)	0.24(24)	0.74(72)	0.14	0.86	0.76	0.24	1.32	0.21	0.00(1.00)
	H₂	23	0.04(1)	0.09(2)	0.87(20)	0.09	0.91	0.84	0.16	1.19	0.15	4.71(0.10)
	所有个体 All individuals	376	0.03(13)	0.26(97)	0.71(266)	0.16	0.84	0.73	0.27	1.38	0.24	1.23(0.54)
SNP4			CC	CT	TT	C	T
	F₁	117	0.27(32)	0.49(57)	0.24(28)	0.52	0.48	0.50	0.50	2.00	0.37	0.07(0.97)
	F₂	143	0.40(56)	0.43(62)	0.17(25)	0.61	0.39	0.52	0.48	1.91	0.36	1.16(0.56)
	H₁	99	0.31(31)	0.55(54)	0.14(14)	0.59	0.41	0.51	0.49	1.94	0.37	1.52(0.47)
	H₂	23	0.35(8)	0.35(8)	0.30(7)	0.52	0.48	0.50	0.50	2.00	0.37	2.11(0.35)
	所有个体 All individuals	382	0.33(127)	0.47(181)	0.20(74)	0.57	0.43	0.51	0.49	1.96	0.37	0.44(0.80)
SNP5			AA	AG	GG	A	G
	F₁	106	0.14(15)	0.60(63)	0.26(28)	0.44	0.56	0.51	0.49	1.97	0.37	4.53(0.10)
	F₂	136	0.20(27)	0.46(63)	0.34(46)	0.43	0.57	0.51	0.49	1.96	0.37	0.41(0.81)
	H₁	91	0.11(10)	0.52(47)	0.37(34)	0.37	0.63	0.53	0.47	1.87	0.36	1.10(0.58)
	H₂	23	0.09(2)	0.43(10)	0.48(11)	0.30	0.70	0.58	0.42	1.73	0.33	0.02(0.99)
	所有个体 All individuals	356	0.15(54)	0.52(183)	0.33(119)	0.41	0.59	0.52	0.48	1.94	0.37	1.44(0.49)
SNP6			GG	GA	AA	G	A
	F₁	116	0.60(70)	0.36(41)	0.04(5)	0.78	0.22	0.66	0.34	1.52	0.28	0.11(0.95)
	F₂	140	0.56(78)	0.34(48)	0.10(14)	0.73	0.27	0.60	0.40	1.65	0.32	2.48(0.29)
	H₁	95	0.47(45)	0.41(39)	0.12(11)	0.68	0.32	0.56	0.44	1.77	0.34	0.32(0.85)
	H₂	23	0.52(12)	0.48(11)	0.00(0)	0.76	0.24	0.64	0.36	1.57	0.30	2.27(0.32)
	所有个体 All individuals	374	0.55(205)	0.37(139)	0.08(30)	0.73	0.27	0.61	0.39	1.64	0.31	0.87(0.65)
SNP7			GG	GA	AA	G	A
	F₁	108	0.00(0)	0.31(34)	0.69(74)	0.16	0.84	0.73	0.27	1.36	0.23	3.77(0.15)
	F₂	139	0.00(0)	0.26(36)	0.74(103)	0.13	0.87	0.77	0.23	1.29	0.20	3.08(0.21)
	H₁	91	0.01(1)	0.39(35)	0.60(55)	0.20	0.80	0.68	0.32	1.48	0.27	3.19(0.20)
	H₂	21	0.00(0)	0.19(4)	0.81(17)	0.10	0.90	0.83	0.17	1.21	0.16	0.23(0.89)
	所有个体 All individuals	359	0.01(1)	0.30(109)	0.69(249)	0.15	0.85	0.74	0.26	1.35	0.23	9.37(0.01)

表4

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表7

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表9

表10

表11

图3

表12

表13

表14

SNP2-SNP3和SNP5-SNP6位点单倍型组合与生长性状的关联分析"

月龄 Month age	组合基因型 Combined genotype	体重 Weight (kg)	体高 Body height (cm)	体斜长 Body length (cm)	胸围 Chest girth (cm)
初生 Primary	H1H1H4H4(18)	3.64±0.16bc	34.78±1.04b	34.44±1.28bc	40.83±1.96b
	H1H1H4H5(52)	3.73±0.11bc	35.50±0.64ab	35.87±1.02bc	41.56±1.10b
	H1H1H4H6(62)	3.67±0.09bc	34.71±0.51b	34.44±0.92bc	41.21±1.02b
	H1H1H5H5(29)	4.07±0.18abc	36.28±0.94ab	36.00±1.17bc	44.24±1.88ab
	H1H1H5H6(42)	3.61±0.11bc	33.79±0.62b	33.52±0.91c	42.43±1.20b
	H1H1H6H6(40)	3.40±0.14c	34.43±0.76b	34.28±1.02bc	39.99±1.30b
	H1H2H4H4(16)	3.83±0.24bc	35.00±0.98b	37.56±1.68abc	38.88±2.14b
	H1H2H4H5(30)	3.86±0.16bc	35.23±0.78ab	35.43±1.07bc	42.23±1.76b
	H1H2H4H6(23)	3.58±0.17bc	33.91±1.02b	34.22±1.16bc	41.30±2.06b
	H2H3H4H4(6)	4.62±0.25a	38.83±0.48a	41.67±0.84a	49.67±3.81a
	H2H3H4H5(5)	4.18±0.22ab	37.40±1.50ab	36.80±1.66abc	43.80±2.80ab
	H2H3H4H6(8)	4.14±0.37ab	36.38±0.94ab	39.63±2.17ab	43.38±3.39ab
	H2H2H4H4(12)	4.00±0.18abc	35.83±1.35ab	37.17±1.38abc	41.08±1.88b
3月龄 Three months	H1H1H4H4(15)	24.61±0.68	54.73±1.15	57.93±0.95ab	71.20±1.05
	H1H1H4H5(30)	22.89±0.56	53.63±0.68	56.03±0.77ab	68.50±0.82
	H1H1H4H6(46)	24.34±0.43	48.60±1.61	51.21±1.82b	70.30±0.62
	H1H1H5H5(13)	22.98±0.55	53.62±0.87	58.08±0.88ab	68.23±0.74
	H1H1H5H6(25)	23.90±0.49	53.68±0.61	57.44±0.67ab	68.88±0.89
	H1H1H6H6(23)	24.37±0.60	54.09±0.57	56.13±0.63ab	72.00±1.09
	H1H2H4H4(9)	25.12±1.09	55.33±1.15	59.56±1.43a	72.11±1.81
	H1H2H4H5(19)	25.21±0.61	53.63±0.71	57.18±1.03ab	69.21±1.01
	H1H2H4H6(14)	24.19±0.61	54.64±0.70	57.43±0.78ab	69.79±1.08
	H2H3H4H4(4)	24.65±2.05	51.75±1.31	57.00±1.29ab	72.75±2.84
	H2H3H4H5(3)	23.67±1.94	54.00±0.00	58.00±2.08ab	71.00±3.46
	H2H3H4H6(4)	23.80±0.50	53.50±0.87	56.50±1.32ab	68.75±0.75
	H2H2H4H4(6)	25.83±1.06	55.33±1.54	59.17±2.20a	71.67±2.39

表14

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项目 Item	检测到的位点 Detected loci
SNPs	42
Indel	3
内含子区Intron region	31
外显子区Exon region	5
UTR3	9

SNPs	突变类型 Mutation type	上游引物序列 Forward primer sequence	下游引物序列 Reverse primer sequence	延伸引物序列 Extended primer sequence
SNP1	218921728 C>G	ACGTTGGATGATGAAACTCCCCAGTAAATG	ACGTTGGATGTGAAGAAGACATGTCCCCTG	TCTGAAACTCCCCAGTAAATGTTATCA
SNP2	218921837 C>G	ACGTTGGATGAGACTGTCCTGGGAACATAG	ACGTTGGATGTACAGTGTAGTTAGGAGACC	TTTCTTAGGTACTTGATCAAT
SNP3	218921856 A>G	ACGTTGGATGAGACTGTCCTGGGAACATAG	ACGTTGGATGTACAGTGTAGTTAGGAGACC	TACCTAGTTGGGGGATTC
SNP4	218922172 C>T	ACGTTGGATGACGTTCTGGTTCTGGAACTG	ACGTTGGATGTGAAGGATGTGAAGGTCAGC	GGTGAAGAGCAGGGCCTTGTCGTTC
SNP5	218922461 A>G	ACGTTGGATGCCTTCAATCCCAGTGATTCC	ACGTTGGATGTTCTGTTCCTTGGGTCACTG	CCCCCTCCCAGTGATTCCAGTTTCACC
SNP6	218922482 G>A	ACGTTGGATGCCTTCAATCCCAGTGATTCC	ACGTTGGATGTTCTGTTCCTTGGGTCACTG	TGAGGTCTTCTTGTTCTCTAGGTCT
SNP7	218923544 G>A	ACGTTGGATGAATGCCTGCCATCCAACCTG	ACGTTGGATGTTCTACTGCTGCTAGCCCTG	TCTCCTGCTGCTAGCCCTGCCCAGT

月龄 Month age	群体 Group	数量 Number	体重Weight (kg)			体高Body height (cm)			体斜长Body length (cm)			胸围Chest girth (cm)
月龄 Month age	群体 Group	数量 Number	CC	CG	GG	CC	CG	GG	CC	CG	GG	CC	CG	GG
初生 Primary	F₁	115	3.42±0.09	3.44±0.11	3.82±0.17	34.16±0.59	33.78±0.71	35.83±1.01	33.45±0.72	32.22±1.02	34.83±2.20	39.15±1.06ab	38.25±1.39b	45.17±1.68a
	F₂	141	3.68±0.08	3.72±0.14	3.90±0.40	34.29±0.43	34.36±0.78	36.00±3.00	34.45±0.58	35.22±1.18	34.00±1.00	40.98±0.82	41.22±1.45	45.00±5.00
	H₁	99	4.14±0.10	4.25±0.10	/	36.90±0.45	37.30±0.38	/	38.40±0.71	40.00±1.05	/	45.56±0.93	45.46±1.02	/
	H₂	23	4.16±0.29	3.68±0.29	3.50	37.00±1.05	36.17±1.66	34.00	37.75±1.35a	34.00±0.93b	31.00	45.13±1.98	42.67±3.36	46.00
	所有个体 All individuals	378	3.81±0.06	3.84±0.08	4.07±0.26	35.52±0.32	35.49±0.43	36.77±1.42	35.76±0.45	35.90±0.62	36.20±2.04	42.55±0.61	42.18±0.83	47.68±2.73
3月龄 Three months	F₁	78	25.00±0.67	26.24±0.87	28.26±1.87	54.53±0.54	56.55±0.70	54.03±1.50	58.69±0.44	59.16±0.57	59.62±1.21	70.34±0.86	71.81±1.11	74.14±2.41
	F₂	80	25.14±0.50	24.83±0.86	23.85±2.71	54.46±0.42	54.26±0.71	56.03±2.25	57.47±0.51	55.65±0.88	56.50±2.78	70.88±0.68	71.30±1.16	69.02±3.67
	H₁	45	27.87±0.87	27.37±1.12	/	54.36±0.58	54.52±0.75	/	58.41±0.64	58.27±0.82	/	74.09±1.02	75.92±1.30	/
	H₂	23	27.97±1.42	25.82±2.24	-	54.30±0.65	52.50±1.04	-	58.52±0.98	55.44±1.56	-	73.70±1.68	70.24±2.70	-
	所有个体 All individuals	217	26.29±0.43	26.53±0.58	28.22±1.60	54.25±0.33	54.91±0.44	54.06±1.22	58.19±0.35	57.55±0.46	58.51±1.29	72.02±0.55	73.05±0.74	73.85±2.06

月龄 Month age	群体 Group	数量 Number	体重Weight (kg)			体高Body height (cm)			体斜长Body length (cm)			胸围Chest girth (cm)
月龄 Month age	群体 Group	数量 Number	CC	CG	GG	CC	CG	GG	CC	CG	GG	CC	CG	GG
初生 Primary	F₁	112	3.58±0.48	3.46±0.15	3.42±0.07	33.00±4.00	34.10±1.15	34.02±0.48	35.00±5.00	33.30±1.16	32.82±0.65	39.50±11.50	37.75±2.24	39.40±0.88
	F₂	134	4.11±0.21	3.77±0.12	3.60±0.09	37.00±1.50	34.46±0.55	34.14±0.54	38.00±1.25	35.48±0.74	33.97±0.77	42.43±1.84	41.92±1.22	40.44±0.94
	H₁	95	3.75±0.75	4.37±0.17	4.13±0.08	31.50±2.50b	37.46±0.55a	37.13±0.38a	33.50±5.50	40.33±1.13	38.62±0.73	39.00±4.00	45.71±1.71	45.75±0.78
	H₂	23	4.50	5.30±1.30	3.85±0.21	42.00	41.50±3.50	35.90±0.80	43.00	42.50±7.50	35.55±0.90	39.00	53.50±13.50	43.90±1.40
	所有个体 All individuals	364	3.99±0.28ab	4.22±0.15a	3.75±0.06b	35.88±1.53	36.88±0.82	35.30±0.31	37.38±2.19ab	37.90±1.17a	35.24±0.44b	39.98±2.96	44.72±1.58	42.37±0.60
3月龄 Three months	F₁	77	-	28.25±1.32	25.30±0.57	-	55.19±1.05	54.98±0.46	-	59.61±0.87	58.77±0.38	-	73.32±1.70	70.67±0.74
	F₂	78	24.50±2.19	25.78±0.67	24.54±0.56	56.75±1.83	54.55±0.56	54.23±0.47	58.03±2.27	57.86±0.69	56.31±0.59	72.00±2.99	70.50±0.92	71.18±0.77
	H₁	44	-	27.61±1.39	27.91±0.83	-	54.80±0.91	54.26±0.55	-	59.46±0.92	58.20±0.56	-	74.74±1.61	74.75±0.98
	H₂	14	35.80	-	26.71±1.02	56.00	-	53.62±0.57	60.00	-	57.46±1.00	82.00	-	72.00±1.34
	所有个体 All individuals	213	28.32±2.17	27.71±0.67	26.08±0.41	56.81±1.65	54.66±0.50	54.28±0.31	59.59±1.74	59.05±0.53	57.70±0.32	75.60±2.82	72.80±0.87	72.16±0.53

月龄 Month age	群体 Group	数量 Number	体重Weight (kg)			体高Body height (cm)			体斜长Body length (cm)			胸围Chest girth (cm)
月龄 Month age	群体 Group	数量 Number	AA	AG	GG	AA	AG	GG	AA	AG	GG	AA	AG	GG
初生 Primary	F₁	112	3.58±0.48	3.46±0.14	3.42±0.07	33.00±4.00	34.06±1.17	34.02±0.48	35.00±5.00	33.47±1.07	32.82±0.65	39.50±11.50	37.47±2.11	39.40±0.88
	F₂	139	4.11±0.21	3.79±0.12	3.61±0.09	37.00±1.50	34.46±0.55	34.05±0.53	38.00±1.25	35.46±0.75	33.94±0.75	42.43±1.84	41.96±1.24	40.38±0.94
	H₁	98	3.75±0.75	4.37±0.17	4.13±0.08	31.50±2.50b	37.46±0.55a	37.07±0.37a	33.50±5.50	40.33±1.13	38.71±0.71	39.00±4.00	45.71±1.71	45.58±0.75
	H₂	23	4.50	5.30±1.30	3.85±0.21	42.00	41.50±3.50	35.90±0.80	43.00	42.50±7.50	35.55±0.90	39.00	53.50±13.50	43.90±1.40
	所有个体 All individuals	369	4.08±0.23	3.97±0.09	3.78±0.06	36.45±1.23	35.83±0.48	35.38±0.30	37.79±1.75	36.82±0.69	35.39±0.43	41.91±2.38	43.01±0.94	42.43±0.59
3月龄 Three months	F₁	75	-	28.25±1.32a	25.30±0.57b	-	55.19±1.05	54.98±0.46	-	59.61±0.87	58.77±0.38	-	73.32±1.70	70.67±0.74
	F₂	80	24.55±2.48	25.76±0.76	24.14±0.63	56.75±1.83	54.55±0.56	54.23±0.47	58.03±2.27	57.86±0.69	56.31±0.59	72.00±2.99	70.50±0.92	71.18±0.77
	H₁	45	-	27.52±1.40	27.74±0.82	-	54.79±0.90	54.29±0.54	-	59.41±0.98	57.97±0.58	-	74.72±1.60	74.80±0.96
	H₂	14	35.80	-	26.71±1.02	56.00	-	53.62±0.57	60.00	-	57.46±1.00	82.00	-	72.00±1.34
	所有个体 All individuals	214	28.28±2.18	27.71±0.67	26.04±0.41	56.82±1.65	54.66±0.50	54.28±0.31	59.53±1.76	59.04±0.54	57.64±0.33	75.61±2.82	72.80±0.86	72.17±0.53