中国农业科学 ›› 2021, Vol. 54 ›› Issue (14): 3077-3087.doi: 10.3864/j.issn.0578-1752.2021.14.013

• 园艺 • 上一篇    下一篇

两种微生物菌剂对有机基质袋培秋黄瓜产量、品质及根际环境的影响

王君正(),张琪,高子星,马雪强,屈锋,胡晓辉()   

  1. 西北农林科技大学园艺学院/农业农村部西北设施园艺工程重点实验室,陕西杨凌 712100
  • 收稿日期:2020-08-30 接受日期:2020-11-24 出版日期:2021-07-16 发布日期:2021-07-26
  • 通讯作者: 胡晓辉
  • 作者简介:王君正,E-mail: wjz20190915@163.com
  • 基金资助:
    陕西省重点研发计划(2019TSLNY01-05);陕西省农业科技创新转化项目(NYKJ-2019-YL02);陕西省农业科技创新转化项目(NYKJ-2020-YL-09);西北农林科技大学试验示范站(基地)科技创新与成果转化项目(TGZX2019-23)

Effects of Two Microbial Agents on Yield, Quality and Rhizosphere Environment of Autumn Cucumber Cultured in Organic Substrate

WANG JunZheng(),ZHANG Qi,GAO ZiXing,MA XueQiang,QU Feng,HU XiaoHui()   

  1. College of Horticulture, Northwest A&F University/Key Laboratory of Protected Horticultural Engineering in Northwest, Ministry of Agriculture and Rural Affairs, Yangling 712100, Shaanxi
  • Received:2020-08-30 Accepted:2020-11-24 Online:2021-07-16 Published:2021-07-26
  • Contact: XiaoHui HU

摘要:

【目的】探究甲基营养型芽孢杆菌和植物乳杆菌两种微生物菌剂对有机基质培黄瓜产量、品质及根际环境的影响,开发新型功能型微生物制剂,指导黄瓜优质高效生产。【方法】以‘博耐526’黄瓜品种为材料,采用有机基质袋培方式,在浇灌清水(N)和营养液(F)2种养分水平下,以不添加菌剂为对照(分别记为CK1和CK2),于定植后第20、35和50天按2.5×1010CFU/株的菌剂用量分别灌根添加‘VL-10’型甲基营养型芽孢杆菌(NT1、FT1)和‘LYS-1’型植物乳杆菌(NT2、FT2)两种菌剂,共6个处理。【结果】两种养分水平下,与CK1和CK2处理相比,甲基营养型芽孢杆菌处理植株的干物质积累量增幅分别为13.51%和15.02%,产量增幅分别为20.83%和15.63%;植物乳杆菌处理植株干物质积累量增幅分别为11.43%和8.42%,产量增幅分别为17.42%和14.96%,甲基营养型芽孢杆菌对植株干物质积累和产量形成的促进效果优于植物乳杆菌。以FT1处理的果实品质最优,其游离氨基酸、有机酸、可溶性糖、还原糖和维C含量分别较CK2处理显著提高了10.61%、28.93%、22.92%和39.88%(P<0.05),FT2处理的果实品质次之;NT1和NT2处理较CK1处理显著提高了植株P(7.43%和13.50%)、K(10.60%和8.19%)元素积累量(P<0.05),FT1和FT2处理的植株N(19.57%和24.18%)、P(17.16%和12.50%)、K(16.48%和26.25%)元素积累量均较CK2处理显著提高(P<0.05);FT1处理的N、P、K肥料利用率较CK2处理分别显著提高了82.85%、483.90%和75.60%(P<0.05),FT2处理分别显著提高了102.42%、367.98%和120.46%(P<0.05)。NT1和NT2处理较CK1处理提高了盛果期的基质蔗糖酶(100.66%、116.60%)、过氧化氢酶(3.39%、4.10%)和碱性磷酸酶(6.99%、95.08%)活性,其中过氧化氢酶和碱性磷酸酶活性在拉秧期依然高于CK1处理;而FT1和FT2处理均较CK2处理显著提高了盛果期和拉秧期的基质脲酶(3.75%、4.95%和13.13%、6.12%)、蔗糖酶(68.62%、24.93%和31.68%、63.35%)和碱性磷酸酶活性(18.00%、26.99%和109.64%、84.01%)(P<0.05),且甲基营养型芽孢杆菌对脲酶和碱性磷酸酶的影响优于植物乳杆菌;两种养分水平下,甲基营养型芽孢杆菌处理的基质有效态氮含量在盛果期分别较CK1和CK2显著提高了63.33%和72.70%(P<0.05),在拉秧期增幅为25.48%和86.46%,同时期增幅均大于植物乳杆菌,有利于保持更高的基质有效态氮含量。【结论】灌根添加7.5×1010CFU/株的甲基营养型芽孢杆菌和植物乳杆菌均可改善有机基质袋培黄瓜的根际环境,促进植株对元素的吸收、同化和积累,有效提高黄瓜产量和果实品质,以甲基营养型芽孢杆菌的增产提质效果更优。

关键词: 微生物菌剂, 黄瓜, 基质栽培, 产量, 品质, 根际环境

Abstract:

【Objective】A bag-cultivated experiment was conducted to test the potential of two microbial agents (Bacillus methylotrophicus and Lactobacillus plantarum) on the yield, quality and rhizosphere environment of autumn cucumber using organic substrate as a growth medium. The results of the experiment were helpful to develop the new functional type of microbial agents to guide the high quality and high efficiency production of cucumber. 【Method】Cucumber cultivar ‘Bonai 526’ was sown in a bag of organic substrate. There were a total of six treatments, including CK1 (Irrigation of water and non-application of microbial agents), NT1 (Irrigation of water and application of Bacillus methylotrophicusagents), NT2 (Irrigation of water and application of Lactobacillus plantarumagents), CK2 (Irrigation of nutrient solution and non-application of microbial agents), FT1 (Irrigation of nutrient solution and application ofBacillus methylotrophicusagents), and FT2 (Irrigation of nutrient solution and application of Lactobacillus plantarumagents). The microbial agents (Bacillus methylotrophicus as ‘VL-10’ and Lactobacillus plantarum as ‘LYS-1’) were added to the cucumber rhizosphere at the rate of 2.5×1010 CFU/plant on 20th, 35th and 50th day. 【Result】Compared with CK1 and CK2 treatment,Bacillus methylotrophicus inoculation in both of the nutrient levels increased the dry matter content by 13.51% and 15.02%, and yield by 20.83% and 15.63%, respectively; Lactobacillus plantarum increased the dry matter content by 11.43% and 8.42%, and the yield by 17.42% and 14.96%, respectively. The fruit quality under FT1 treatment was the best; compared with CK2 treatment, its content of free amino acid, organic acid, soluble sugar, reducing sugar and vitamin C were significantly increased by 10.61%, 28.93%, 22.92% and 39.88%, respectively,, followed by FT2 treatment. The accumulation of phosphorus and potassium were significantly higher under both NT1 (7.43% and 10.60%) and NT2 (13.50% and 8.19%) compared with CK1 treatment (P<0.05). Compared with CK2 treatment, the accumulation of nitrogen, and potassium were significantly higher under FT2 (24.18% and 26.25%), while the phosphorus concentration in plants were significantly higher under FT1 (17.16%). Compared with CK2 treatment, the nitrogen, phosphorus and potassium fertilizer utilization rates under FT1 treatment were significantly increased by 82.85%, 483.90% and 75.60% (P<0.05), respectively, which under FT2 treatment was significantly increased by 102.42%, 367.98% and 120.46%, respectively (P<0.05). Compared with CK1 treatment, the treatments NT1 and NT2 improved soil enzymatic activities of sucrase (100.66% and 116.60%), catalase (3.39% and 4.10%) and alkaline phosphatase (6.99% and 95.08%) at the full fruiting stage, among which catalase and alkaline phosphatase activities were still higher at the end of the experiment. Compared with CK2 treatment, the FT1 treatment significantly improved soil enzymatic activities of urease (3.75% and 13.13%), sucrase (68.62% and 31.68%) and alkaline phosphatase (18.00% and 109.64%) in the full fruiting period and the seedling pulling period, while the urease (4.95% and 6.12 %), sucrase (24.93% and 63.35%) and alkaline phosphatase activity (26.99% and 84.01%) under FT2 treatment (P<0.05). The effect ofBacillus methylotrophicagent on the urease and alkaline phosphatase activities were better than that ofLactobacillus plantarumagent. Moreover, the substrate incubated with Bacillus methylotrophic showed significantly higher available nitrogen content (63.33% and 72.70%) (P<0.05) compared with their respective controls at the full fruiting stage, and the increase was 25.48% and 86.46% at the end of the experiment, respectively. 【Conclusion】In conclusion, adding 7.5×1010CFU/plant of Bacillus methylotrophic to the substrate could improve the rhizosphere environment of cucumber and promote the absorption, assimilation and accumulation of essential elements by the plant. Moreover, it could effectively improve the yield and fruit quality of cucumber.

Key words: microbial agents, cucumber, substrate cultivation, yield, fruit quality, rhizosphere environment