中国农业科学 ›› 2020, Vol. 53 ›› Issue (22): 4700-4709.doi: 10.3864/j.issn.0578-1752.2020.22.016
陈俐静1,陈卓1,李娜1,孙亚伟1,李红波2,宋雯雯1,张杨2(),姚刚1(
)
收稿日期:
2019-12-11
接受日期:
2020-05-29
出版日期:
2020-11-16
发布日期:
2020-11-28
通讯作者:
张杨,姚刚
作者简介:
陈俐静,E-mail:基金资助:
CHEN LiJing1,CHEN Zhuo1,LI Na1,SUN YaWei1,LI HongBo2,SONG WenWen1,ZHANG Yang2(),YAO Gang1(
)
Received:
2019-12-11
Accepted:
2020-05-29
Online:
2020-11-16
Published:
2020-11-28
Contact:
Yang ZHANG,Gang YAO
摘要: 【目的】探明新疆褐牛与国外优良品种安格斯牛产肉性能及肉品质差异、体脂代谢相关基因及其产物表达的品种间的差异性。【方法】选择相同饲养管理条件下24月龄新疆褐牛和安格斯牛进行屠宰试验,测定其胴体性状和肉品质性状;采集背最长肌和皮下脂肪,采用石蜡切片技术观测脂肪组织和肌肉组织形态学差异;利用实时荧光定量PCR检测脂肪酸合成酶(fatty acid synthesase,FAS)、脂肪酸结合蛋白4(fatty acid-binding protein 4,FABP4)、激素敏感酯酶(hormone-sensitive lipase,HSL)、脂蛋白脂酶(lipoprotein lipase,LPL)和瘦素(Leptin,LEP)基因mRNA表达量;采用蛋白免疫印迹(Western blot)测定FAS、HSL、LPL蛋白表达水平。【结果】1)新疆褐牛净肉率极显著高于安格斯牛(P<0.01),而背膘厚度显著低于安格斯牛(P<0.05)。其余所测定的胴体性状相关指标均无品种间差异(P>0.05)。2)新疆褐牛肉色L*显著低于安格斯牛(P<0.05);新疆褐牛平均肌纤维直径和横截面积均显著大于安格斯牛(P<0.05);新疆褐牛皮下脂肪组织单位面积脂肪细胞个数极显著多于安格斯(P<0.01),其余所测定的肉品质性状相关指标均无品种间差异(P>0.05)。3)新疆褐牛与安格斯牛背最长肌和皮下脂肪FAS、FABP4、HSL、LPL、LEP等5个基因mRNA表达量均差异不显著(P>0.05);新疆褐牛背最长肌组织中FAS蛋白表达量极显著低于安格斯牛(P<0.01);HSL蛋白表达量显著低于安格斯牛(P<0.05);新疆褐牛皮下脂肪组织中HSL蛋白表达量显著低于安格斯牛(P<0.05)。【结论】研究发现新疆褐牛产肉性能较好;但安格斯牛肉色较亮,且嫩度较好,体肪沉积能力较强。两品种间脂代谢相关基因产物FAS和HSL蛋白差异可能与安格斯肉牛背膘厚度差异有关。
陈俐静,陈卓,李娜,孙亚伟,李红波,宋雯雯,张杨,姚刚. 新疆褐牛与安格斯牛胴体及肉质性状及脂代谢相关基因表达差异比较[J]. 中国农业科学, 2020, 53(22): 4700-4709.
CHEN LiJing,CHEN Zhuo,LI Na,SUN YaWei,LI HongBo,SONG WenWen,ZHANG Yang,YAO Gang. Comparison of the Carcass and Beef Quality Traits with the Expression of the Lipid Metabolism Related Genes Between Xinjiang Brown Cattle and Angus Beef Cattle[J]. Scientia Agricultura Sinica, 2020, 53(22): 4700-4709.
表1
引物信息"
基因名称 Gene name | 序列5′-3′ Sequence | 产物大小 Product size(bp) |
---|---|---|
LEP | F: CTTCAGTGGATGGTCCCTCG | 148 |
R: AATGGCAGGTTGGTGGGAAA | ||
FAS | F: TGAGACAGACCCGAAGTCCT | 127 |
R: CTCCTCGGGCTTGTCTTGTT | ||
FABF4 | F: AGATGGTGCTGGAATGTGTCA | 103 |
R: GGAGTTCGATGCAAACGTCA | ||
HSL | F: AGAGTGCTTCTATGCCTACTGC | 117 |
R: GACACGGTGAAGCAGAGGTTC | ||
LPL | F: CCCGGCTTTGATATTGGGAAG | 142 |
R: TCAGGGACTTGTCATGGCATT | ||
Beta-actin | F: CATGTACGTTGCTATCCAGGC | 250 |
R: CTCCTTAATGTCACGCACGAT |
Table 3
Comparison of carcass traits between XBC and ABC"
性状 Traits | 单位 Unit | XBC | ABC | P值 P value |
---|---|---|---|---|
活体重Body weight | kg | 704.0±29.90 | 798.0±33.60 | 0.077 |
胴体重Carcass weight | kg | 423.0±15.60 | 459.0±28.60 | 0.306 |
净肉重Net meat weight | kg | 320.0±11.40 | 300.0±23.90 | 0.464 |
骨重Bone weight | kg | 69.7±3.96 | 75.6±2.73 | 0.251 |
屠宰率Slaughter rate | % | 60.3±2.28 | 59.4±0.41 | 0.735 |
净肉率Net meat rate | % | 45.6±1.50A | 39.9±0.60B | 0.010 |
肉骨比Meat bone ratio | % | 4.6±0.18 | 4.0±0.31 | 0.097 |
肌间脂肪Intermuscular fat | % | 6.98±1.36 | 8.8±0.84 | 0.278 |
背膘厚度Backfat thickness | cm | 1.0±0.10a | 1.7±0.22b | 0.021 |
眼肌面积Loin eye muscle area | cm2 | 92.7±3.09 | 98.1±3.35 | 0.261 |
表4
XBC和ABC部分肉品质性状比较"
性状 Traits | 单位 Unit | XBC | ABC | P 值 P value |
---|---|---|---|---|
肉色L*值 Meat Color-L* | - | 28.69±2.06a | 31.43±2.15b | 0.032 |
肉色a*值 Meat Color-a* | - | 11.99±5.87 | 15.91±3.29 | 0.149 |
肉色b*值 Meat Color-b* | - | 3.64±1.86 | 4.60±1.37 | 0.296 |
灰分Ash | % | 1.56±0.78 | 1.05±0.27 | 0.131 |
水分Muscle moisture | % | 74.20±2.61 | 73.2±2.69 | 0.505 |
肌内脂肪Intramuscular fat | % | 1.80±0.37 | 1.81±0.17 | 0.993 |
嫩度Tenderness | N | 5.65±1.52 | 5.78±1.91 | 0.922 |
[1] | 周靖航, 李鹏, 刘丽元, 赵国丽, 黄锡霞, 乃比江, 夏热普·居马衣, 谈锐, 史远刚. 新疆褐牛种质资源现状与群体遗传改良建议. 中国畜牧杂志, 2017, 53(8): 38-43. |
ZHOU J H, LI P, LIU L Y, ZHAO G L, HUANG X X, NAI B J, XIAREP·JU MAYI, TAN R, SHI Y G. Status of Xinjiang Brown Cattle Germplasm Resources and Suggestions for Population Genetic Improvement.Chinese Journal of Animal Science, 2017, 53(8): 38-43. (in Chinese) | |
[2] | 昝林森, 梅楚刚, 王洪程. 我国肉牛产业经济发展形势及对策建议. 西北农林科技大学学报(社会科学版), 2015, 15(6): 48-52. |
ZAN L S, MEI C G, WANG H C.Economic situation and countermeasures of beef cattle industry in China.Journal of Northwest A&F University (Social Science Edition), 2015, 15(6): 48-52. (in Chinese) | |
[3] | 李劲, 孙有江. 高档牛肉生产技术探讨. 中国畜牧业, 2014(21): 81-82. |
LI J, SUN Y J.Discussion on production technology of high-grade beef.China Animal Industry, 2014(21): 81-82 .(in Chinese) | |
[4] |
GONZALEZ J M, PHELPS K J.United States beef quality as chronicled by the National Beef Quality Audits, Beef Consumer Satisfaction Projects, and National Beef Tenderness Surveys-A review.Asian-Australasian Journal of Animal Sciences, 2018, 31(7): 1036-1042.
doi: 10.5713/ajas.18.0199 pmid: 29879824 |
[5] |
COSTA ANA S H, LOPES PAULA A, ESTEVAO M,MARTINS S V, ALVES S P, PINTO R A, PISSARRA H, CORREIA J J, PINHO M, FONTES C M G A, PRATES J A M. Contrasting cellularity and fatty acid composition in fat depots from Alentejana and Barrosa bovine breeds fed high and low forage diets.International Journal of Biological Sciences, 2012, 8(2): 214-227.
doi: 10.7150/ijbs.8.214 pmid: 22253565 |
[6] | 姜贝贝. 过瘤胃甜菜碱对湖羊生长性能和体脂肪沉积调控的研究[D]. 扬州: 扬州大学, 2017. |
JIANG B B.Regulation function of rumen protected betaine on growth performance and body fat deposition of Hu lambs[D]. Yangzhou: Yangzhou University, 2017. (in Chinese) | |
[7] |
FAUSTMAN C, SUN Q, MANCINI R, MANCINI R, SUMAN S P.Myoglobin and lipid oxidation interactions: mechanistic bases and control.Meat Science, 2010, 86(1): 86-94.
doi: 10.1016/j.meatsci.2010.04.025 |
[8] |
REARDON W, MULLEN A M, SWEENEY T, HAMILL R M.Association of polymorphisms in candidate genes with color, water- holding capacity, and composition traits in bovine M. Longissimus and M. semimembranosus.Meat Science, 2010, 86(2): 270-275.
doi: 10.1016/j.meatsci.2010.04.013 |
[9] | 顾悦, 张保军, 高爱武. 家畜肌内脂肪沉积过程相关基因研究进展. 食品与发酵工业, 2014, 40(8): 159-162. |
GU Y, ZHANG B J, GAO A W.Research progress of genes related to intramuscular fat deposition in livestock.Food and Fermentation Industries, 2014, 40(8): 159-162. (in Chinese) | |
[10] | BONNET M, FAULCONNIER Y, LEROUX C, JURIE C, CASSAR-MALEK I, BAUCHART D, BOULESTEIX P, PETHICK D, HOCQUETTE J F, CHILLIARD Y.Glucose-6-phosphate dehydrogenase and leptin are related to marbling differences among Limousin and Angus or Japanese Black × Angus steers.Journal of Animal Science, 2007(85): 2882-2894. |
[11] |
SHENKEL F S, MILLER S P, YE X,MOORE S S,NKRUMAH J D,LI C, YU J, MANDELL I B, WILTON J W, WILLIAMS J L.Association of single nucleotide polymorphisms in the leptin gene with carcass and meat quality traits of beef cattle.Journal of animal science, 2005, 83(9): 2009-2020.
doi: 10.2527/2005.8392009x pmid: 16100055 |
[12] |
HUANG H L, ZHANG Y, CAO M Y, XUE L U, SHEN W L.Effects of fasting on the activities and mRNA expression levels of lipoprotein lipase (LPL), hormone-sensitive lipase (HSL) and fatty acid synthetase (FAS) in spotted seabass Lateolabrax maculatus.Fish Physiology and Biochemistry, 2018, 44(1): 387-400.
doi: 10.1007/s10695-017-0442-4 pmid: 29147968 |
[13] |
HILLER B, HERDMANN A, NUERNBERG K.Dietary n-3 fatty acids significantly suppress lipogenesis in bovine muscle and adipose tissue: a functional genomics approach.Lipids, 2011, 46(7): 557-567.
doi: 10.1007/s11745-011-3571-z |
[14] |
JEONG J, KWON E G, IM S K, SEO K S, BAIL M.Expression of fat deposition and fat removal genes is associated with intramuscular fat content in longissimus dorsi muscle of Korean cattle steers.Journal of Animal Science, 2012, 90(6): 2044-2053.
doi: 10.2527/jas.2011-4753 |
[15] |
PANNIER L, MULLEN A M, HAMILL R M, STAPLETON P C, SWEENEY T.Association analysis of single nucleotide polymorphisms in DGAT1, TG and FABP4 genes and intramuscular fat in crossbred Bos Taurus cattle. Meat Science, 2010, 85(3): 515-518.
doi: 10.1016/j.meatsci.2010.02.025 |
[16] |
LEHNERT S A, REVETER A, BYRNE K A, WANG Y H, NATTRASS G S, HUDSON N J, GREENWOOD P L.Gene expression studies of developing bovine longissimus muscle from two different beef cattle breeds. BMC Developmental Biology, 2007, 7(1): 95.
doi: 10.1186/1471-213X-7-95 |
[17] |
CHO S, PARK T, YOON D, CHEONG H S, NAMGOONG S, PARK B L, LEE H W, HAN C S, KIM E M, CHEONG I, KIM H, SHIN H D.Identification of genetic polymorphisms in FABP3 and FABN and putative association with back fat thickness in Korean native cattle.BMB Reports, 2008, 41(1): 29-34.
doi: 10.5483/bmbrep.2008.41.1.029 pmid: 18304447 |
[18] | 梁忠泉, 刘畅, 杨志娜, 谢永新, 袁昌隆. 脂肪组织石蜡切片制作方法探讨. 中国组织化学与细胞化学杂志, 2019, 28(2): 170-173. |
LIANG Z Q, LIU C, YANG Z N, XIE Y X, YUAN C L.Discussion on preparation method for paraffin section of adipose tissues.Chinese Journal of Histochemistry and Cytochemistry, 2019, 28(2): 170-173. (in Chinese) | |
[19] | 王艳秀. FastStone Capture应用于图书馆信息服务探析. 图书馆学研究, 2011, 10(20): 70-72. |
WANG Y X.Analysis of FastStone Capture in library information service.Research on Library Science, 2011, 10(20): 70-72. (in Chinese) | |
[20] | 王洪星, 张雨良, 罗志文, 杨文君, 刘志昕. ScYLV和SrMV的PCR引物优化设计. 中国糖料, 2012(1): 16-20, 24. |
WANG H X, ZHANG Y L, LUO Z W, YANG W J, LIU Z X.Optimized design of PCR primers for ScYLV and SrMV.Sugar Crops of China, 2012(1): 16-20, 24. (in Chinese) | |
[21] | 张明. 安格斯与西门塔尔牛杂交一代育肥性能及肉品质研究[D]. 兰州: 甘肃农业大学, 2016. |
ZHANG M.Study on fattening performance and meat quality of Angus and Simmental[D]. Lanzhou: Gansu Agricultural University. 2016. (in Chinese) | |
[22] | 王国富, 吴慧光, 赵新海, 季守财, 吴红君, 王东升, 刘胜敏, 高树新. 安格斯牛、海福特牛和中国西门塔尔牛的部分胴体性状比较分析. 内蒙古民族大学学报, 2010, 25(5): 535-537. |
WANG G F, WU H G, ZHAO X H, JI S C, WU H J, WANG D S, LIU S M, GAO S X.Comparative study on some carcass traits in Angus, Hereford and Chinese Simmental Cattles.Journal of Inner Mongolia University for Nationalities, 2010, 25(5): 535-537. (in Chinese) | |
[23] |
HE L, WU H, WANG G, MENG Q, ZHOU Z.The effects of including corn silage, corn stalk silage, and corn grain in finishing ration of beef steers on meat quality and oxidative stability.Meat Science, 2018, 139: 142-148.
doi: 10.1016/j.meatsci.2018.01.023 pmid: 29413674 |
[24] | 王莉, 孙宝忠, 保善科, 孔祥颖, 谢鹏, 张丽, 余力群, 李海鹏. 补饲和放养牦牛肉品质及肌肉微观结构差异. 肉类研究, 2015, 29(6): 5-10. |
WANG L, SUN B Z, BAO S K, KONG X Y, XIE P, ZHANG L, YU L Q, LI H P.Differences in quality and muscle microstructure of supplemented and stocked yak meat.Meat Research, 2015 , 29(6): 5-10.(in Chinese) | |
[25] | 崔国梅, 彭增起, 靳红果, 孟晓霞, 冯云. 黑色牛肉与正常色泽牛肉理化性状及凝胶特性的对比分析. 食品科学, 2011, 32(13): 106-109. |
CUI G M, PENG Z Q, JIN H G, MENG X X, FENG Y.A comparative study of physico-chemical properties and gel properties of dark firm dry (dfd) beef and normal colored beef.Food Science, 2011, 32(13): 106-109. (in Chinese) | |
[26] | 胡猛, 张文举, 尹君亮, 陈宁, 张云峰. 育成荷斯坦奶公牛与其他3个品种牛的肉品质比较研究. 中国畜牧兽医, 2013, 40(3): 95-99. |
HU M, ZHANG W J, YIN J L, CHEN N, ZHANG Y F.Comparative Study on Meat Quality of Holstein Milk Bulls and Three Other Breeds.China Animal Husbandry & Veterinary Medicine, 2013, 40(3): 95-99. (in Chinese) | |
[27] |
闫向民, 张金山, 李红波, 李娜, 杜玮, 周振勇, 张杨. 不同月龄新疆褐牛阉牛胴体性状及肉品质比较研究. 中国畜牧兽医, 2015, 42(11): 2954-2960.
doi: 10.16431/j.cnki.1671-7236.2015.11.020 |
YAN X M, ZHANG J S, LI H B, LI N, DU W, ZHOU Z Y, ZHANG Y.Comparative study on carcass characteristics and meat quality of Xinjiang Brown Cattle and yak in different months of age.China Animal Husbandry & Veterinary Medicine, 2015, 42(11): 2954-2960. (in Chinese)
doi: 10.16431/j.cnki.1671-7236.2015.11.020 |
|
[28] | 曹芝, 敖日格乐, 王纯洁, 王小梅. 不同杂交品种肉牛背最长肌纤维组织学结构与嫩度对比研究. 肉类研究, 2012, 26(2): 1-3. |
CAO Z, AO R G L, WANG C J, WANG X M. Comparative study on the histological structure and tenderness of the longest dorsal muscle fiber of different hybrid beef cattle.Meat Research, 2012, 26(2): 1-3. (in Chinese) | |
[29] | 杨公社, 邱怀, 路兴中. 猪体脂肪形成的细胞学和形态学研究. 西北农业大学学报, 1997, 25(1): 15-21. |
YANG G S, QIU H, LU X Z.Cytological and morphological study on the formation of pig aunt.Journal of Northwest Agricultural University, 1997, 25(1): 15-21. (in Chinese) | |
[30] | ALBRECHT E, GOTOH T, EBARA F, XU J X, VIERGUTE T, NRNBERG G, MAAK S, WEGNER J.Cellular conditions for intramuscular fat deposition in Japanese Black and Holstein steers.Meat Science, 2011(89): 13-20. |
[31] | 王鑫, 李光鹏. 牛肉质性状及其影响因素. 动物营养学报, 2019, 31(11): 4949-4958. |
WANG X, LI G P.Beef quality traits and its influencing factors.Chinese Journal of Animal Nutrition, 2019, 31(11): 4949-4958. (in Chinese) | |
[32] | 魏胜娟. 牛FABP4基因代谢功能研究及品种间脂肪细胞发育特性分析[D]. 杨凌: 西北农林科技大学, 2014. |
WEI S J.Metabolic Function research on Bovine FABP4 gene and analysis of varietal features on adipocyte development[D]. Yangling: Northwest Agriculture and Forestry University, 2014. (in Chinese) | |
[33] | 曲桂娟, 董晓庆, 孟轲音, 杨连玉, 丁尚红, 秦贵信. 不同杂交组合肉牛背最长肌FAS mRNA表达及其对肌内脂肪沉积的影响. 中国兽医学报, 2016, 36(7): 1183-1185, 1211. |
QU G J, DONG X Q, MENG K Y, YANG L Y, DING S H, QIN G X.FAS mRNA expression of different hybridized combination beef cattle and its effect on intramuscular fat deposition. Chinese Journal of Veterinary Science, 2016, 36(7): 1183-1185, 1211. (in Chinese) | |
[34] | 戢爽. 肉牛脂肪组织和背最长肌脂代谢相关基因差异表达分析[D]. 吉林: 吉林大学, 2013. |
JI S.Analysis of differentially expressed gene related to lipid metabolism between adipose tissues and longissimus dorsi muscle[D]. Jilin: Jilin Agricultural University, 2013 .(in Chinese) | |
[35] |
REN M, WEGNER J, BELLMANM O, BROCKMANN G, SCHNEIDER F, TEUSCHER F, ENDER F, ENDER K.Comparing mRNA levels of genes encoding leptin, leptin receptor, and lipoprotein lipase between dairy and beef cattle.Domestic Animal Endocrinology, 2002, 23: 371-381.
doi: 10.1016/S0739-7240(02)00179-0 |
[36] | 赵称赫. 蒙古牛肉品质及背最长肌脂肪代谢相关基因mRNA表达量的研究[D]. 呼和浩特: 内蒙古农业大学, 2016. |
ZHAO C H.Study on meat quality and mRNA expression of longissimus dorsi muscle fatty metabolism related factors of Mongolia Cattle[D]. Huhehaote: Neimenggu Agriculture University, 2016. (in Chinese) | |
[37] |
栾兆进, 刘开东, 贺建宁, 程明, 曲绪仙, 柳楠. 绵羊FAM134B、PPARγ、HSL和FAS基因表达量及与肌内脂肪含量的关系. 畜牧兽医学报, 2016, 47(12): 2379-2389.
doi: 10.11843/j.issn.0366-6964.2016.12.007 |
LUAN Z J, LIU K D, HE J N, CHENG M, QU X X, LIU N.SheepFAM134B, PPARγ, HSL and FAS gene expression levels and their relationship with intramuscular trace content. Chinese Journal of Animal and Veterinary Sciences, 2016, 47(12): 2379-2389. (in Chinese)
doi: 10.11843/j.issn.0366-6964.2016.12.007 |
|
[38] | 王刚, 曾勇庆, 武英, 魏述东, 包新见, 刘婵娟, 孙延晓. 猪肌肉组织LPL基因表达的发育性变化及其与肌内脂肪沉积关系的研究. 畜牧兽医学报, 2007, 7(3): 253-257. |
WANG G, ZENG Y Q, WU Y, WEI S D, BAO X J, LIU C J, SUN Y X.The developmental changes oflpl mrna expression in muscle and their association with intramuscular fat for pigs. Chinese Journal of Animal and Veterinary Sciences, 2007, 7(3): 253-257.(in Chinese) |
[1] | 宋淑珍, 高良霜, 李宏, 宫旭胤, 刘立山, 魏玉兵. 相对饲养水平对绵羊肌肉组织结构及肌纤维组成相关基因的影响[J]. 中国农业科学, 2022, 55(21): 4304-4314. |
[2] | 刘旺景,唐德富,敖长金. 沙葱及其提取物对小尾寒羊生产性能、胴体特征、肉品质和血清生化指标的影响[J]. 中国农业科学, 2022, 55(17): 3461-3472. |
[3] | 胡斐斐,钱书意,黄峰,姜薇,强宇,江峰,胡海梅,李侠,张春晖. 低压静电场辅助短期冻藏对猪肉品质的影响[J]. 中国农业科学, 2021, 54(9): 1993-2005. |
[4] | 张兰,王良治,黄艳玲,廖秀冬,张丽阳,吕林,罗绪刚. 饲粮微量元素添加模式对肉仔鸡生长和胴体性能及肌肉品质的影响[J]. 中国农业科学, 2021, 54(22): 4906-4916. |
[5] | 张美琦,李妍,李树静,高艳霞,李建国,曹玉凤,李秋凤. 饲粮能量水平对荷斯坦阉牛生产性能、血液指标、屠宰性能及肉品质的影响[J]. 中国农业科学, 2021, 54(1): 203-212. |
[6] | 李雪茹,师希雄,王建忠,张攀高,田铸,韩玲. 一氧化氮合成酶抑制剂对宰后成熟过程中牦牛肉品质的影响[J]. 中国农业科学, 2020, 53(8): 1617-1626. |
[7] | 赵洋洋,李妍,韩永胜,王晓玲,李建国,高艳霞,曹玉凤,李秋凤. 谷饲饲粮粗蛋白质水平对荷斯坦公牛屠宰性能和肉品质的影响[J]. 中国农业科学, 2020, 53(2): 431-439. |
[8] | 刘丽元,周靖航,张梦华,李金霞,方季青,谭世新,王爱芳,黄锡霞,王雅春. 新疆褐牛基因外显子多态性及其与SCS和泌乳性状关联性分析[J]. 中国农业科学, 2017, 50(13): 2592-2603. |
[9] | 朱洪龙,杨 杰,徐小波,潘孝青,秦 枫,李 健,徐业飞,周晓云,顾洪如. 饲养方式对猪宰前生理行为、胴体性质及肉品质的影响[J]. 中国农业科学, 2016, 49(22): 4441-4450. |
[10] | 赵忠海,李 辉,易恒洁,杨胜林,彭邦星,卜小雁. MyoG和MEF2a基因多态性聚合效应对鸭屠宰性状的影响[J]. 中国农业科学, 2016, 49(18): 3649-3661. |
[11] | 田月珍,冯文,王雅春,黄锡霞,俞英. 新疆褐牛乳中体细胞数与产奶性状的影响因素分析[J]. 中国农业科学, 2016, 49(12): 2437-2448. |
[12] | 邢焕,栾素军,孙永波,萨仁娜,张宏福. 舍内不同氨气浓度对肉鸡抗氧化性能及肉品质的影响[J]. 中国农业科学, 2015, 48(21): 4347-4357. |
[13] | 薛山, 贺稚非, 李洪军. 伊拉兔宰后肌糖原变化及其与兔肉品质的相关性[J]. 中国农业科学, 2014, 47(4): 814-822. |
[14] | 王春青1, 李侠1, 张春晖1, 陈旭华1, 孙红梅1, 李银1, 李海1, 何雷堂2. 肌原纤维特性与鸡肉原料肉品质的关系[J]. 中国农业科学, 2014, 47(10): 2003-2012. |
[15] | 蒋守群, 蒋宗勇, 郑春田, 林映才, 洪平, 陈芳, 阮栋. 饲粮代谢能和粗蛋白水平对黄羽肉鸡生产性能 和肉品质的影响[J]. 中国农业科学, 2013, 46(24): 5205-5216. |
|