中国农业科学 ›› 2020, Vol. 53 ›› Issue (10): 2045-2054.doi: 10.3864/j.issn.0578-1752.2020.10.011
收稿日期:
2019-09-20
接受日期:
2019-12-19
出版日期:
2020-05-16
发布日期:
2020-05-22
通讯作者:
王晓闻
作者简介:
延莎,E-mail:yanshawell@163.com。
基金资助:
YAN Sha,XING JieWen,WANG XiaoWen()
Received:
2019-09-20
Accepted:
2019-12-19
Online:
2020-05-16
Published:
2020-05-22
Contact:
XiaoWen WANG
摘要:
【目的】 比较不同菌种发酵对藜麦蛋白质水解及脂质变化的影响,为藜麦发酵食品的开发提供理论参考。【方法】 以藜麦为原料,加入不同菌种(酵母菌、植物乳杆菌及其混合菌)进行发酵,通过测定蛋白水解指数、蛋白质各组分、游离氨基酸、游离脂肪酸及脂质构成来判定发酵效果,确认哪种微生物更有利于藜麦营养成分的转化。【结果】 经酵母、植物乳杆菌、酵母和植物乳杆菌混合菌发酵后,藜麦蛋白质水解指数均有所升高,且主要是清蛋白和球蛋白发生了水解。发酵后藜麦中的游离氨基酸含量增加显著,尤其是必需氨基酸。酵母发酵藜麦更有利于蛋白质、多肽等水解成氨基酸。通过发酵处理,藜麦中的游离脂肪酸含量显著增加,且饱和脂肪酸、单不饱和脂肪酸、多不饱和脂肪酸的比例更为接近1﹕1﹕1;甘油三酯、甘油二酯含量降低,但磷脂等功能脂的含量有所提高,且有酵母菌参与更有利于上述转化。【结论】 酵母菌、植物乳杆菌发酵过程中主要水解藜麦清蛋白和球蛋白成氨基酸等小分子化合物,且游离必需氨基酸含量显著升高;两种菌发酵后都有利于增加游离脂肪酸含量,降低甘油三酯、甘油二酯含量,且提高磷脂的含量。经酵母发酵,更有利于改善藜麦营养。
延莎,邢洁雯,王晓闻. 不同菌种发酵对藜麦蛋白质特性及脂质构成的影响[J]. 中国农业科学, 2020, 53(10): 2045-2054.
YAN Sha,XING JieWen,WANG XiaoWen. Effects of Different Strain Fermentation on Protein Hydrolysis and Lipid Profile of Quinoa[J]. Scientia Agricultura Sinica, 2020, 53(10): 2045-2054.
表1
发酵对藜麦游离氨基酸含量的影响"
氨基酸 Amino acids (mg?kg-1) | Q | QY | QL | QM |
---|---|---|---|---|
必需氨基酸 Essential amino acids | ||||
苏氨酸 Threonine | 101.42±2.06 | 2199.18±8.53 | 815.74±3.07 | 1051.65±4.73 |
缬氨酸 Valine | 174.99±2.74 | 3384.52±8.77 | 1705.11±6.91 | 2319.03±6.54 |
甲硫氨酸 Methionine | 122.37±1.86 | 1533.06±6.39 | 1047.83±4.85 | 1355.92±3.06 |
异亮氨酸 Isoleucine | 143.02±2.05 | 3080.71±9.03 | 1692.01±5.23 | 2242.94±7.22 |
亮氨酸 Leucine | 255.84±4.29 | 4719.57±9.75 | 3232.84±7.86 | 4536.63±10.54 |
苯丙氨酸 Phenylalanine | 159.70±1.72 | 3070.32±8.48 | 1886.72±4.72 | 2720.13±6.18 |
赖氨酸 Lysine | 141.19±1.53 | 943.36±5.09 | 862.52±5.68 | 977.09±5.02 |
色氨酸 Tryptophan | 236.05±5.07 | 2796.41±10.04 | 2109.33±7.03 | 2619.24±7.06 |
总必需氨基酸 Total essential amino acid | 1334.58±21.32d | 21727.13±66.08a | 13352.10±45.35c | 17822.63±50.35b |
非必需氨基酸 Nonessential amino acids | ||||
天冬氨酸 Aspartic acid | 488.50±6.93 | 3547.80±7.82 | 1935.12±8.28 | 3329.41±8.35 |
谷氨酸 Glutamic acid | 925.82±5.74 | 4049.22±9.86 | 2777.75±6.09 | 3387.20±8.19 |
半胱氨酸 Cysteine | 40.36±0.75 | 343.30±2.59 | 213.93±1.07 | 125.78±1.05 |
丝氨酸 Serine | 77.40±1.03 | 1744.23±6.03 | 472.15±2.64 | 1025.13±4.82 |
甘氨酸 Glycine | 136.90±2.84 | 2498.26±8.74 | 963.47±4.91 | 1777.93±5.03 |
组氨酸 Histidine | 193.23±2.79 | 682.02±3.06 | 456.23±2.08 | 741.49±2.84 |
精氨酸 Arginine | 742.21±3.62 | 4979.91±10.23 | 3868.34±11.03 | 5676.42±10.96 |
丙氨酸 Alanine | 193.41±3.91 | 3133.15±8.39 | 1231.01±9.75 | 2615.62±4.67 |
脯氨酸 Proline | 168.91±2.94 | 2023.48±6.08 | 780.49±5.36 | 1247.71±3.80 |
酪氨酸 Tyrosine | 172.65±3.82 | 2491.18±7.74 | 1486.96±8.19 | 2219.03±6.49 |
总非必需氨基酸 Total non-essential amino acid | 3139.39±34.37d | 25492.55±70.54a | 14185.45±59.40c | 22145.72±56.20b |
总氨基酸 Total amino acid | 4473.97±55.69d | 47219.68±136.62a | 27537.55±104.75c | 39968.35±106.55b |
表2
发酵对藜麦游离脂肪酸含量的影响"
脂肪酸 Fatty acids (mg?kg-1) | Q | QY | QL | QM |
---|---|---|---|---|
饱和脂肪酸 Saturated fatty acids | ||||
C8:0 | 0.58±0.03 | 2.69±0.33 | 0.90±0.04 | 3.62±0.24 |
C10:0 | 0.43±0.02 | 13.76±0.79 | 0.87±0.04 | 7.65±0.33 |
C12:0 | 3.40±0.12 | 37.73±1.83 | 12.48±0.51 | 19.34±0.61 |
C13:0 | 0.72±0.03 | 1.76±0.03 | 0.97±0.05 | 1.18±0.24 |
C14:0 | 69.46±1.09 | 296.47±4.38 | 224.87±4.09 | 259.17±3.78 |
C15:0 | 22.45±0.64 | 94.19±2.05 | 78.32±1.73 | 85.64±1.52 |
C16:0 | 4482.64±11.26 | 21018.07±16.78 | 16171.65±15.82 | 17657.56±15.94 |
C17:0 | 17.48±0.85 | 74.50±4.02 | 57.84±3.64 | 72.57±4.75 |
C18:0 | 344.31±2.36 | 2279.72±9.61 | 897.62±8.52 | 2191.76±8.92 |
C20:0 | 169.04±1.82 | 551.17±4.93 | 496.99±5.06 | 561.57±4.86 |
C21:0 | 13.95±0.78 | 55.08±0.85 | 47.87±0.73 | 52.67±1.93 |
C22:0 | 308.46±3.06 | 1305.70±8.42 | 1108.93±7.68 | 1208.67±8.72 |
C23:0 | 21.02±0.61 | 99.40±2.06 | 87.62±1.06 | 93.69±0.85 |
C24:0 | 134.28±1.05 | 696.46±4.59 | 554.77±3.28 | 623.36±2.63 |
总饱和脂肪酸 Total saturated fatty acid | 5588.22±23.72d | 26526.70±60.67a | 19741.70±52.25c | 22838.45±55.32b |
单不饱和脂肪酸 Monounsaturated fatty acids | ||||
C14:1 | 0.59±0.04 | 2.33±0.72 | 0.86±0.05 | 2.82±0.54 |
C16:1 | 40.73±0.88 | 1354.02±4.83 | 100.49±1.19 | 1640.35±6.39 |
C17:1 | 13.55±0.57 | 38.50±1.04 | 31.19±0.96 | 51.44±0.84 |
C18:1N9C | 10579.78±19.52 | 37318.61±24.05 | 32149.23±29.71 | 36904.41±23.61 |
C20:1 | 589.41±8.72 | 1281.06±7.92 | 1236.96±8.04 | 1438.67±9.88 |
C22:1N9 | 2034.98±12.3 | 5628.81±8.80 | 3735.53±6.92 | 3701.20±7.42 |
C24:1 | 174.28±1.09 | 689.84±4.19 | 807.97±5.36 | 976.22±6.92 |
总单不饱和脂肪酸 Total monounsaturated fatty acid | 13433.32±43.12d | 46313.17±51.55a | 38062.23±52.23c | 44715.11±55.6b |
多不饱和脂肪酸 Polyunsaturated fatty acids | ||||
C18:2N6C | 26213.13±22.87 | 76635.37±26.08 | 74195.18±25.82 | 75988.65±26.09 |
C18:3N3 | 4353.54±9.51 | 10777.47±12.39 | 10502.01±13.86 | 11222.60±13.62 |
C20:2 | 55.19±1.02 | 162.08±4.71 | 152.79±3.08 | 175.64±2.08 |
C20:3N3 | 2.83±0.36 | 11.09±0.82 | 9.71±0.77 | 10.33±0.84 |
C22:2 | 80.59±2.44 | 288.43±4.74 | 218.54±3.14 | 208.64±3.09 |
总多不饱和脂肪酸 Total polyunsaturated fatty acid | 30705.28±36.2d | 87874.44±48.74a | 85078.23±46.67c | 87605.86±45.72b |
总脂肪酸 Total fatty acid | 49726.82±103.04d | 160714.30±160.96a | 142882.20±151.15c | 155159.40±156.64b |
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