谷子抗锈病反应相关MYB转录因子的鉴定与表达

doi:10.3864/j.issn.0578-1752.2019.22.007

中国农业科学 ›› 2019, Vol. 52 ›› Issue (22): 4016-4026.doi: 10.3864/j.issn.0578-1752.2019.22.007

谷子抗锈病反应相关MYB转录因子的鉴定与表达

白辉¹,宋振君²,王永芳¹,全建章¹,马继芳¹,刘磊¹,李志勇¹(),董志平¹()

1 河北省农林科学院谷子研究所/国家谷子改良中心/河北省杂粮重点实验室,石家庄 050035
2 上海电子信息职业技术学院通信与信息工程学院,上海 201411

收稿日期:2019-08-01 接受日期:2019-09-30 出版日期:2019-11-16 发布日期:2019-11-16
通讯作者: 李志勇,董志平
作者简介:白辉,E-mail：baihui_mbb@126.com
基金资助:
国家重点研发计划(2018YFD1000703);国家重点研发计划(2018YFD1000700);国家自然科学基金(31872880);河北省农林科学院创新工程(2019-4-2-3);国家现代农业产业技术体系(CARS-07-13.5-A8)

Identification and Expression Analysis of MYB Transcription Factors Related to Rust Resistance in Foxtail Millet

BAI Hui¹,SONG ZhenJun²,WANG YongFang¹,QUAN JianZhang¹,MA JiFang¹,LIU Lei¹,LI ZhiYong¹(),DONG ZhiPing¹()

1 Institute of Millet Crops, Hebei Academy of Agriculture and Forestry Sciences/National Foxtail Millet Improvement Center/Minor Cereal Crops Laboratory of Hebei Province, Shijiazhuang 050035
2 College of Communication and Information Engineering, Shanghai Technical Institute of Electronic & Information, Shanghai 201411;

Received:2019-08-01 Accepted:2019-09-30 Online:2019-11-16 Published:2019-11-16
Contact: ZhiYong LI,ZhiPing DONG

摘要/Abstract

摘要：

【目的】谷子锈病是影响其产量和品质的重要因素之一。鉴定谷子抗锈病相关的MYB转录因子,为谷子抗锈病机理研究奠定基础。【方法】利用real-time PCR技术,检测9个SiMYBs转录因子基因在（1）谷子孕穗期根、茎、叶和穗4个部位的表达情况,（2）在谷子抗（resistance,R）、感（susceptibility,S）锈病反应120 h内的表达丰度差异,（3）在植株外接水杨酸（salicylic acid,SA）和茉莉酸甲酯（methyl jasmonate,MeJA）后24 h内的表达变化;比较SiMYBs基因在谷子R、S、SA与MeJA 4种反应中的表达模式;选择抗病相关SiMYBs基因进行转录激活活性检测和亚细胞定位。【结果】 SiMYB100在叶部表达量最高,其余8个基因均在根部表达量最高,SiMYB074最显著。5个基因的表达与抗病相关：SiMYB074和SiMYB202受锈菌侵染诱导表达,抗病反应早期的表达量明显高于感病反应;SiMYB041和SiMYB177在抗病反应早期下调表达,后期上升至接种前,而感病反应中保持低水平表达;SiMYB100在接种后的48 h内,抗病、感病反应表达模式相反。在响应外源激素SA和MeJA反应中,SiMYBs基因的表达量均发生不同程度的变化。4个基因（SiMYB074、SiMYB100、SiMYB174和SiMYB202）在R、SA与MeJA反应中的表达模式一致,不同于S反应。5个抗病相关SiMYBs基因具有转录激活活性,其编码蛋白质定位于细胞核中。【结论】鉴定到SiMYB041、SiMYB074、SiMYB100、SiMYB177和SiMYB202 5个基因的表达与谷子抗锈病相关;SiMYB074与SiMYB100在谷子生长发育和抗病过程中都发挥一定的功能;SiMYB074、SiMYB100、SiMYB174和SiMYB202 4个基因可能通过SA和JA信号途径参与谷子的早期抗病反应。

关键词: 谷子, MYB转录因子, 锈病, real-time PCR, 抗病反应

Abstract:

【Objective】 Millet rust is one of the important factors affecting the yield and quality of foxtail millet. Identification of MYB transcription factors related to rust resistance in foxtail millet lays a foundation for the study of the mechanism of rust resistance in foxtail millet. 【Method】 In this study, we used real-time PCR to detect the expression patterns of 9 MYB transcription factors (1) in roots, stems, leaves and panicles at booting stage; (2) during 120 hours post-inoculated with Uromyces setariae-italicae urediniospores in resistance (R) and susceptible (S) reactions; (3) during 24 hours after treatment with salicylic acid (SA) and methyl jasmonate (MeJA) in foxtail millet. Then their expression patterns in four reactions of R, S, SA and MeJA were compared, and the resistance-related MYB transcription factors were selected for detection of transactivation activity and subcellular localization. 【Result】 The highest expression of SiMYB100 was in leaves, and the highest expression of the other eight genes, especially SiMYB074, was in roots. The expression of five genes was correlated with disease resistance. SiMYB074 and SiMYB202 were induced by rust fungus infection and their expression levels at early stage of disease resistance were significantly higher than that in the susceptible reaction. The expression of SiMYB041 and SiMYB177 was down-regulated in the early stage of resistance reaction and increased to pre-inoculation level in the later stage, while their expression remained low in susceptible reaction. SiMYB100 showed opposite expression pattern in resistance and susceptible responses. In response to exogenous SA and MeJA, the expression of SiMYBs gene changed in varying degrees. The expression patterns of four genes (SiMYB074, SiMYB100, SiMYB174 and SiMYB202) in the R, SA and MeJA reactions were identical, but different from the S reaction. Five resistance-related SiMYBs genes have the transactivation activity and their encoding proteins are located in the nucleus. 【Conclusion】 The expression of five genes, SiMYB041, SiMYB074, SiMYB100, SiMYB177 and SiMYB202, was identified to be associated with resistance to rust disease in foxtail millet. SiMYB074 and SiMYB100 play certain roles in the growth and disease resistance of foxtail millet. Four genes, SiMYB074, SiMYB100, SiMYB174 and SiMYB202, may participate in early disease resistance of foxtail millet through SA and JA signaling pathways.

Key words: Setaria italica, MYB transcription factors, rust disease, real-time PCR, disease resistance

白辉, 宋振君, 王永芳, 全建章, 马继芳, 刘磊, 李志勇, 董志平. 谷子抗锈病反应相关MYB转录因子的鉴定与表达[J]. 中国农业科学, 2019, 52(22): 4016-4026.

BAI Hui, SONG ZhenJun, WANG YongFang, QUAN JianZhang, MA JiFang, LIU Lei, LI ZhiYong, DONG ZhiPing. Identification and Expression Analysis of MYB Transcription Factors Related to Rust Resistance in Foxtail Millet[J]. Scientia Agricultura Sinica, 2019, 52(22): 4016-4026.

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谷子抗锈病反应相关MYB转录因子的鉴定与表达

Identification and Expression Analysis of MYB Transcription Factors Related to Rust Resistance in Foxtail Millet

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