中国农业科学 ›› 2019, Vol. 52 ›› Issue (1): 1-10.doi: 10.3864/j.issn.0578-1752.2019.01.001
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
陈竟男1,2(),马晓兰1,王振3,李仕金1,谢皓2(
),叶兴国1,林志珊1(
)
收稿日期:
2018-07-01
接受日期:
2018-09-19
出版日期:
2019-01-01
发布日期:
2019-01-12
通讯作者:
谢皓,林志珊
基金资助:
CHEN JingNan1,2(),MA XiaoLan1,WANG Zhen3,LI ShiJin1,XIE Hao2(
),YE XingGuo1,LIN ZhiShan1(
)
Received:
2018-07-01
Accepted:
2018-09-19
Online:
2019-01-01
Published:
2019-01-12
Contact:
Hao XIE,ZhiShan LIN
摘要:
【目的】 探究从前苏联引进的簇毛麦No.1026(Dv#4)的EST-SSR序列特征及其在染色体的分布,分析它们在不同簇毛麦间及与小麦间的多态性,为其进一步的研究与利用提供依据。【方法】通过Illumina HiSeq测序获得No.1026植株的转录组序列,利用MISA软件分析转录组SSR序列及特征,采用Primer 3设计SSR引物,随机合成238对引物,对小麦中国春与簇毛麦Dv#4和引自英国剑桥的簇毛麦Dv#2的基因组DNA进行扩增,在琼脂糖凝胶上分离评价扩增产物的多态性,并利用一套小麦-簇毛麦异染色体系进行扩增分析。【结果】 检测了No.1026总长62.76 Mb的转录组序列,发现10 497个SSR位点,它们分布于8 735条Unigene上。在1—6个核苷酸的重复单元中,单、双、三碱基的重复占95.85%,其中三核苷酸串联重复数量最多,占SSR总数的50.33%,而CCG/CGG基序的重复占三核苷酸串联重复的41.66%;单核苷酸重复是第二大类型,出现频率为27.13%,其中A/T重复占单核苷酸重复的74.58%。二核苷酸重复类型的数量位列第三,占SSR总数的18.39%。在238对EST-SSR引物中,88对在中国春与簇毛麦(包括Dv#2和Dv#4)之间的扩增产物显示多态性;8对只在簇毛麦和单一异染色体系扩增;4对可在簇毛麦和多个异染色体系中特异扩增;但多数可在簇毛麦中清晰扩增的引物不能在任何异染色体系中扩增,推测可能与簇毛麦基因组及染色体导入小麦过程中的变异有关;47对(19.74%)在2份不同来源的簇毛麦Dv#2和Dv#4之间的扩增产物呈现多态性。利用1对EST-SSR引物和1个EST-PCR标记检测48个簇毛麦植株,证明多态性的SSR引物可有效用于检测簇毛麦的异质性。【结果】 簇毛麦No.1026(Dv#4)的转录组中存在丰富的SSR序列,其中CCG/CGG、A/T和AG/CT等三核苷酸、单核苷酸和二核苷酸是其最主要的串联重复基序。部分EST-SSR的侧翼保守序列与单一或若干外源染色体特异相关联,据此开发特异的分子标记,可用于跟踪检测小麦背景中特异的簇毛麦染色体或染色体片段。Dv#4与Dv#2间的部分EST-SSR具有多态性,提示2份不同来源簇毛麦的表达序列间存在一定程度的遗传差异,因此,簇毛麦Dv#4值得进一步的发掘研究。
陈竟男,马晓兰,王振,李仕金,谢皓,叶兴国,林志珊. 基于簇毛麦No.1026转录组的SSR序列分析及其PCR标记开发[J]. 中国农业科学, 2019, 52(1): 1-10.
CHEN JingNan,MA XiaoLan,WANG Zhen,LI ShiJin,XIE Hao,YE XingGuo,LIN ZhiShan. SSR Sequences and Development of PCR Markers Based on Transcriptome of Dasypyrum villosum No.1026[J]. Scientia Agricultura Sinica, 2019, 52(1): 1-10.
表1
Dv#4的EST-SSR构成"
重复单元大小 Repeat unit size | 构成比例 Composition ratio (%) | 重复基序(位点数) Repeat motif (locus number) | 重复次数 Repeats |
---|---|---|---|
单核苷酸Mononucleotide | 27.13 | A/T(2124)、C/G(724) | 10—23 |
双核苷酸Dinucleotide | 18.39 | AG/CT(1054)、AC/GT(546)、AT/AT(173)、CG/CG(157) | 6—12 |
三核苷酸Trinucleotide | 50.33 | CCG/CGG(2201)、AGG/CCT(1003)、AGC/CTG(756)、 ACC/GGT(347)、AAG/CTT(322)、ACG/CGT(265)、ATC/ATG(159)、AAC/GTT(135)、AAT/ATT(48)、 ACT/AGT(47) | 5—10 |
四核苷酸Tetranucleotide | 3.75 | AGGG/CCCT(46)、ACGC/GCGT (40)、ATCC/ATGG(37)、 AAGG/CCTT(35)、AGGC/GCCT(23)、ACAT/ATGT(21)、 AGCG/CGCT(21)、AAAG/CTTT(20)、AAAT/ATTT(17)、 AGCC/CTGG(16)、ACGG/CCGT(16)、CCCG/CGGG(15)、 AAAC/GTTT(14)、AGAT/ATCT(13)、AATC/ATTG(10)、 CCGG/CCGG(7)、AACC/GGTT(6)、ACAG/CTGT(5)、 AGCT/AGCT(4)、ATCG/ATCG(3)、ACTG/AGTC(3)、 ACTC/AGTG(3)、AATG/ATTC(3)、AAGC/CTTG(3)、 ATGC/ATGC(2)、ACGT/ACGT(2)、ACCG/CGGT(2)、 ACCC/GGGT(2)、AAGT/ACTT(2)、AACG/CGTT(2)、 ACCT/AGGT(1) | 5—6 |
五核苷酸Pentanucleotide | 0.34 | AGAGG/CCTCT(5)、AGAGC/CTCTG(4)、AAGGG/CCCTT(2) 、ACAGC/CTGTG(2)、ACCAG/CTGGT(2)、AGGCG/CCTCG(2)、 ATCCC/ATGGG(2)、AAAAG/CTTTT(1)、AAACC/GGTTT(1)、 AAACT/AGTTT(1)、AAATC/ATTTG(1)、AAGCT/AGCTT(1)、 AAGGC/CCTTG(1)、AATAC/ATTGT(1)、ACACC/GGTGT(1)、ACCCC/GGGGT(1)、ACCGC/CGGTG(1)、ACGAG/CGTCT(1)、 ACGGC/CCGTG(1)、ACTAG/AGTCT(1)、AGCCC/CTGGG(1)、 AGCCG/CGGCT(1)、AGGGG/CCCCT(1)、ATCCG/ATCGG(1) | 5—6 |
六核苷酸 Hexanucleotide | 0.06 | AAAGCC/GGCTTT(3)、AAACGC/CGTTTG(1)、AATGCC/ATTGGC(1)、ACCAGC/CTGGTG(1)、ACCAGC/CTGGTG(1) | 6—8 |
[1] | GRĄDZIELEWSKA A . The genus Dasypyrum-part 2. Dasypyrum villosum- a wild species used in wheat improvement. Euphytica, 2006,152:441-454. |
[2] | 赵万春, 董剑, 陈其皎, 李晓燕, 高翔, 石引刚, 陈良国 . 簇毛麦—用于小麦改良的一种野生植物. 草业科学, 2012,29(10):1613-1621. |
ZHAO W C, DONG J, CHEN Q J, LI X Y, GAO X, SHI Y G, CHEN L G . Advance in Dasypyrum villosum-a valuable wild species used in wheat improvement. Protacultural Science, 2012,29(10):1613-1621. (in Chinese) | |
[3] | CHEN Q, CONNER R L, LI H, LAROCHE A, GRAF R J, KUZYK A D . Expression of resistance to strip rust, powdery mildew and the wheat curl mite in Triticum aestivum×Haynaldia villosa lines. Canadian Journal of Plant Science, 2002,82(2):451-456. |
[4] | 刘大钧, 陈佩度, 吴沛良, 王耀南, 邱伯行, 王苏玲 . 硬粒小麦-簇毛麦双二倍体. 作物学报, 1986,12(3):155-162. |
LIU D J, CHEN P D, WU P L, WANG Y N, QIU B X, WANG S L . Triticum durum-Haynadia villosa amphidiploid. Acta Agronomica Sinica, 1986,12(3):155-162. (in Chinese) | |
[5] | 陈孝, 徐惠君, 杜丽璞, 尚立民, 韩彬, 施爱农, 肖世和 . 利用组织培养技术向普通小麦导入簇毛麦抗白粉病基因的研究. 中国农业科学, 1996,29(5):1-8. |
CHEN X, XU H J, DU L P, SHANG L M, HAN B, SHI A N, XIAO S H . Transfer of gene resistant to powdery mildew from H. villosa to common wheat by tissue culture. Scientia Agricultura Sinica, 1996,29(5):1-8. (in Chinese) | |
[6] | 陈佩度, 周波, 齐莉莉, 刘大钧 . 用分子原位杂交(GISH)鉴定小麦-簇毛麦双倍体、附加系、代换系和易位系. 遗传学报, 1995,22(5):380-386. |
CHEN P D, ZHOU B, QI L L, LIU D J . Identification of wheat- Haynaldia villosa amphiloid, addition, substitution and translocation lines by in situ hybridization using biotin-labelled genomic DNA as a probe. Acta Genetica Sinica, 1995,22(5):380-386. (in Chinese) | |
[7] |
CHEN P D, QI L L, ZHOU B, ZHANG S Z, LIU D J . Development and molecular cytogenetic analysis of wheat- Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theoretical and Applied Genetics, 1995,91:1125-1128.
doi: 10.1007/BF00223930 pmid: 24170007 |
[8] | 齐莉莉, 陈佩度, 刘大钧, 周波, 张守中, 盛宝钦, 向齐君, 段霞渝, 周益林 . 小麦白粉病新抗源——基因Pm21. 作物学报, 1995,21(3):257-262. |
QI L L, CHEN P D, LIU D J, ZHOU B, ZHANG S Z, SHEENG B Q, XIANG Q J, DUAN X Y, ZHOU Y L . The gene Pm21- a new source for resistance to wheat powdery mildew. Acta Agronomica Sinica, 1995,21(3):257-262. (in Chinese) | |
[9] | LI H, CHEN X, XIN Z Y, MA Y Z, XU H J, CHEN X Y, JIA X . Development and identification of wheat- Haynaldia villosa T6DL.6VS chromosome translocation lines conferring resistance to powdery mildew. Plant Breeding, 2005,124:203-205. |
[10] | LIU C, QI L L, LIU W X, ZHAO W C, WILSON J, FRIEBE B, GILL B S . Development of a set of compensating Triticum aestivum- Dasypyrum villosum Robertsonian translocation lines. Genome, 2011,54:836-844. |
[11] |
XING L, HU P, LIU J., WITEK K, ZHOU S, XU J, ZHOU W, GAO L, HUANG Z, ZHANG R, WANG X, CHEN P, WANG H , JONES J D G, KARAFIATOVA M, VRANA J, BARTOS J, DOLEZEL J, TIAN Y, WU Y, CAO A. Pm21 from Haynaldia villosa encodes a CC-NBS-LRR that confers powdery mildew resistance in wheat. Molecular Plant, 2018, doi: 10.1016/j.molp.2018.02.013.
doi: 10.1016/j.molp.2018.02.013 pmid: 29567451 |
[12] |
HE H, ZHU S, ZHAO R, JIANG Z, JI Y, JI J, QIU D, LI H, BIE T . Pm21, encoding a typical CC-NBS-LRR protein, confers broad- spectrum resistance to wheat powdery mildew disease. Molecular Plant, 2018, doi: 10.1016/j.molp.2018.03.004.
doi: 10.1016/j.molp.2018.03.004 |
[13] | ZHANG Q P, LI Q, WANG X E, WANG H Y, LANG S P, WANG Y N, WANG S L, CHEN P D, LIU D J . Development and characterization of a Triticum aestivum-Haynaldia villosa translocation line T4VS·4DL conferring resistance to wheat spindle streak mosaic virus. Euphytica, 2005,145:317-320. |
[14] |
ZHAO R, WANG H, XIAO J, BIE T, CHENG S, JIA Q, YUAN C, ZHANG R, CAO A, CHEN P, WANG X . Induction of 4VS chromosome recombinants using the CS ph1b mutant and mapping of the wheat yellow mosaic virus resistance gene from Haynaldia villosa. Theoretical and Applied Genetics, 2013,126:2921-2930.
doi: 10.1007/s00122-013-2181-y pmid: 23989649 |
[15] |
李淑梅, 徐川梅, 周波, 陈佩度 . 普通小麦-簇毛麦2V染色体端体异附加系的选育与鉴定. 南京农业大学学报, 2009,32(1):1-5.
doi: 10.3321/j.issn:1000-2030.2009.01.001 |
LI S M, XU C M, ZHOU B, CHEN P D . Development and identification of Triticum-Haynadia villosa ditelesomic addition lines involving chromosome 2V of H.villosa. Journal of Nanjing Agricultural University, 2009,32(1):1-5. (in Chinese)
doi: 10.3321/j.issn:1000-2030.2009.01.001 |
|
[16] |
董剑, 杨华, 赵万春, 李晓燕, 陈其皎, 高翔 . 普通小麦中国春-簇毛麦易位系T1DL·1VS和T1DS·1VL的农艺和品质特性. 作物学报, 2013,39(8):1386-1390.
doi: 10.3724/SP.J.1006.2013.01386 |
DONG J, YANG H, ZHAO W C, LI X Y, CHEN Q J, GAO X . Agronomic traits and grain quality of Chinese Spring- Dasypyrum villosum translocation lines T1DL∙1VS and T1DS∙1VL. Acta Agronomica Sinica, 2013,39(8):1386-1390. (in Chinese)
doi: 10.3724/SP.J.1006.2013.01386 |
|
[17] |
张瑞奇, 冯祎高, 侯富, 陈树林, 别同德, 陈佩度 . 普通小麦-簇毛麦T5VS.5DL易位染色体对小麦主要农艺性状、品质和白粉病抗性的遗传效应分析. 中国农业科学, 2015,48(6):1041-1051.
doi: 10.3864/j.issn.0578-1752.2015.06.01 |
ZHANG R Q, FENG Y G, HOU F, CHEN S L, BIE T D, CHEN P D . The genetic effect of wheat-H. villosa T5VS.5DL translocated chromosome on agronomic characteristics, quality, and powdery mildew resistance of common wheat. Scientia Agricultura Sinica, 2015,48(6):1041-1051. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2015.06.01 |
|
[18] |
ZHANG R, SUN B, CHEN J, CAO A, XING L, FENG Y, LAN C, CHEN P . Pm55, a developmental-stage and tissue-specific powdery mildew resistance gene introgressed from Dasypyrum villosum into common wheat. Theoretical and Applied Genetics, 2016,129:1975-1984.
doi: 10.1007/s00122-016-2753-8 pmid: 27422445 |
[19] |
QI L L, PUMPHREY M O, FRIEBE B, ZHANG P, QIAN C, BOWDEN R L, ROUSE M N, JIN Y, GILL B S . A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theoretical and Applied Genetics, 2011,123(1):159-167.
doi: 10.1007/s00122-011-1574-z pmid: 21437597 |
[20] |
LI H J, CONNER R L, CHEN Q, JIA X, LI H, GRAF R J, LAROCHE A, KUZYK A D . Different reactions to the wheat curl mite and wheat streak mosaic virus in various wheat-Haynaldia villosa 6V and 6VS lines. Plant Disease, 2002,86(4):423-428.
doi: 10.1094/PDIS.2002.86.4.423 |
[21] | LIU C, Ye X G, Wang M J, LI S J, LIN Z S . Genetic behavior of Triticum aestivum-Dasypyrum villosum translocation chromosomes T6V#4S·6DL and T6V#2S·6AL. Journal of Integrative Agriculture, 2017,16(10):2136-2144. |
[22] |
张云龙, 王美蛟, 张悦, 褚翠萍, 林志珊, 徐琼芳, 叶兴国, 陈孝, 张宪省 . 不同簇毛麦6VS 染色体臂的白粉病抗性特异功能标记的开发及应用. 作物学报, 2012,38(10):1827-1832.
doi: 10.3724/SP.J.1006.2012.01827 |
ZHANG Y L, WANG M J, ZHANF Y, CHU C P, LIN Z S, XU Q F, YE X G, CHEN X, ZHANG X S . Development and application of functional markers specific to powdery mildew resistance on chromosome arm 6VS from different origins of Haynaldia villosa. Acta Agronomica Sinica, 2012,38(10):1827-1832. (in Chinese)
doi: 10.3724/SP.J.1006.2012.01827 |
|
[23] | LIN Z S, ZHANG Y L, WANG K, LI J R, XU Q F, CHEN X, ZHANG X S, YE X G . Isolation and molecular analysis of genes Stpk-V2 and Stpk-V3 homologous to powdery mildew resistance gene Stpk-V in a Dasypyrum villosum accession and its derivatives. Journal of Applied Genetics, 2013,54:417-426. |
[24] |
BIE T D, ZHAO R H, ZHU S Y, CHEN S L, HE H G . Development and characterization of marker MBH1 simultaneously tagging genes Pm21 and PmV conferring resistance to powdery mildew in wheat. Molecular Breeding, 2015,35:189.
doi: 10.1007/s11032-015-0385-3 |
[25] |
刘畅, 李仕金, 王轲, 叶兴国, 林志珊 . 簇毛麦6VS特异转录序列P21461及P33259的获得及其分子标记在鉴定小麦-簇毛麦抗白粉病育种材料中的应用. 作物学报, 2017,43(7):983-992.
doi: 10.3724/SP.J.1006.2017.00983 |
LIU C, LI S J, WANG K, YE X G, LIN Z S . Developing of specific transcription sequences P21461 and P33259 on Dasypyrum villosum 6VS and application of molecular markers in identifying wheat-D. villosum breeding materials with powdery mildew resistance. Acta Agronomica Sinica, 2017,43(7):983-992. (in Chinese)
doi: 10.3724/SP.J.1006.2017.00983 |
|
[26] | LI S J, LIN Z S, LIU C, WANG K, DU L P, YE X G . Development and comparative genomic mapping of Dasypyrum villosum 6V#4S- specific PCR markers using transcriptome data. Theoretical and Applied Genetics, 2017,130(10):2057-2068. |
[27] |
PLASCHKE J, GANAL M E, RODER M S . Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theoretical and Applied Genetics, 1995,91:1001-1007.
doi: 10.1007/BF00223912 pmid: 24169989 |
[28] | BRYAN G J, COLLINS A J, STEPHENSON P, ORRY A, SMITH J B, GALE M D . Isolation and characterization of microsatellites from hexaploid bread wheat. Theoretical and Applied Genetics, 1997,94(5):557-563. |
[29] |
谢皓, 陈学珍, 杨柳, 王建立 . EST-SSR标记的发展和在植物遗传研究中的应用. 北京农学院学报, 2005,20(4):73-76.
doi: 10.3969/j.issn.1002-3186.2005.04.018 |
XIE H, CHEN X J, YANG L, WANG J L . Development and application of EST-SSR marker in plant genetic. Journal of Beijing Agricultural College, 2005,20(4):73-76. (in Chinese)
doi: 10.3969/j.issn.1002-3186.2005.04.018 |
|
[30] |
曹亚萍, 曹爱忠, 王秀娥, 陈佩度 . 基于EST-PCR的簇毛麦染色体特异分子标记筛选及应用. 作物学报, 2009,35(1):1-10.
doi: 10.3724/SP.J.1006.2009.00001 |
CAO Y P, CAO A Z, WANG X E, CHEN P D . Screening and application of EST-based PCR markers specific to individual chromosomes of Haynaldia villosa. Acta Agronomica Sinica, 2009,35(1):1-10. (in Chinese)
doi: 10.3724/SP.J.1006.2009.00001 |
|
[31] |
李珊珊, 曾艳飞, 何彩云, 张建国 . 基于沙棘转录组序列开发EST-SSR分子标记. 林业科学研究, 2017,30(1):69-74.
doi: 10.13275/j.cnki.lykxyj.2017.01.010 |
LI S S, ZENG Y F, HE C Y, ZHANG J G . Development of EST-SSR markers based on Seabuckthorn transcriptomic sequences. Forest Research, 2017,30(1):69-74. (in Chinese)
doi: 10.13275/j.cnki.lykxyj.2017.01.010 |
|
[32] | 陈岳, 张微微, 莫海波, 付乃峰, 田代科 . EST-SSR标记构建莲(Nelumbo Adans)遗传连锁图谱. 分子植物育种, 2017,15(6):2265-2273. |
CHEN Y, ZHANG W W, MO H B, FU N F, TIAN D K . Construction of a genetic linkage map of lotus (Nelumbo Adans) using EST-SSR markers. Molecular Plant Breeding, 2017,15(6):2265-2273. (in Chinese) | |
[33] | 陈勋, 龚自明 . 基于EST-SSR 标记的湖北茶树种质资源遗传多样性分析. 分子植物育种, 2017,15(5):1831-1838. |
CHEN X, GONG Z M . Analysis of genetic diversity with EST-SSR markers for tea germplasm in Hubei province. Molecular Plant Breeding, 2017,15(5):1831-1838. (in Chinese) | |
[34] | 李翔, 张颖, 许俊强, 宋婷, 李文璐, 董玉梅, 张应华 . 基于EST-SSR 标记的云南黄瓜种质资源遗传多样性分析. 分子植物育种, 2016,14(5):1179-1188. |
LI X, ZHANG Y, XU J Q, SONG T, LI W L, DONG Y M, ZHANG Y H . Analysis on genetic diversity of local cucumber germplasm resources in Yunnan based on EST-SSR markers. Molecular Plant Breeding, 2016,14(5):1179-1188. (in Chinese) | |
[35] | QI L L, WANG S L, CHEN P D, LIU D J, GILL B S . Identification and physical mapping of three Haynaldia villosa chromosome-6V deletion lines. Theoretical and Applied Genetics, 1998,97(7):1042-1046. |
[36] | ZHANG L D, YUAN D J, YU S W, LI Z G, CAO Y F, MIAO Z Q, QIAN H M, TANG K X . Preference of simple sequence repeats in coding and non-coding regions of Arebidopsis thaliana. Bioinformations, 2004,20(7):1081-1086. |
[37] |
张利达, 唐克轩 . 植物EST-SSR标记开发及其应用. 基因组学与应用生物学, 2010,29(3):534-541.
doi: 10.3969/gab.029.000534 |
ZHANG L D, TANG K X . Development of plant EST-SSR markers and its application. Genomics and Applied Biology, 2010,29(3):534-541. (in Chinese)
doi: 10.3969/gab.029.000534 |
|
[38] |
GAO L F, TANG J F, LI H W, JIA J Z . Analysis of microsatellites in major crops assessed by computational and experimental approaches. Molecular Breeding, 2003,12:245-261.
doi: 10.1023/A:1026346121217 |
[39] |
文亚峰, 韩文军, 周宏, 徐刚标 . 杉木转录组SSR挖掘及EST-SSR标记规模化开发. 林业科学, 2015,51(11):40-49.
doi: 10.11707/j.1001-7488.20151106 |
WEN Y F, HAN W J, ZHOU H, XU G B . SSR mining and development of EST-SSR markers for Cunninghamia lanceolata based on transcriptome sequences. Scientia Silvae Sinicae, 2015,51(11):40-49. (in Chinese)
doi: 10.11707/j.1001-7488.20151106 |
|
[40] |
KASHKUSH K, FELDMAN M, LEVY A A . Silencing and activation in a newly synthesized wheat allotetraploid. Genetics, 2002,160(4):1651-1659.
doi: 10.1017/S0016672301005511 pmid: 11973318 |
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