甘蓝型油菜萝卜细胞质雄性不育恢复材料的创制

doi:10.3864/j.issn.0578-1752.2012.08.002

摘要/Abstract

摘要： 【目的】探讨外源萝卜恢复基因的导入途径与筛选方法，转育甘蓝型油菜萝卜细胞质雄性不育（Ogu CMS）恢复材料，为甘蓝型油菜Ogu CMS育种应用创造条件。【方法】以萝-蓝（Raphanobrassica，2n=58）为供体材料，利用嫁接克服远缘杂交生殖障碍导入外源恢复基因，对获得的属间杂种依次进行甘蓝型油菜性状、自（异）交结实性状以及恢复率稳定性状等特征特性强化筛选。【结果】育成一份甘蓝型油菜Ogu CMS纯合恢复材料CLR650，体细胞染色体数目为38—40条，减数分裂过程中部分细胞存在染色体落后及形成染色体桥等异常现象，但细胞异常对花粉正常形成没有明显影响，恢复株自交、测交均为100%可育。以基于Ogu CMS恢复基因（Rf0）设计的特异分子标记进行检测，证明该材料存在Ogu CMS恢复基因。以与之连锁其它萝卜分子标记分析比较，表明其萝卜片段长度短于国外同类恢复系R113。【结论】CLR650的育成为甘蓝型油菜Ogu CMS创制了新型恢复材料。

关键词: 甘蓝型油菜, 萝-蓝, 嫁接, 远缘杂交, Ogu CMS恢复系

Abstract: 【Objective】 The objective of this study was to explore the radish restore gene transferring and the stable restorer line screening for Ogu CMS in rapeseed.【Method】 The Raphanobrassica (2n=58) as the restoring gene donor and grafting as the restoring gene importing tool was used to obtain the hybrid of Ogu CMS in B. napus. And the stable restoring materials were bred through selection of the seed setting rate in single plant and the restoring ratio in populations from every selfing and backcross generations. Also the selected materials were detected by the cytological observation and the molecular marker screening. 【Result】With the high strength selection, a stable homozygous restoring line named as CLR650 of Ogu CMS in B. napus was selected. This line can restore the male fertility in both progenies of self-pollination and testcross at a rate of 100%. Although it’s somatic chromosome numbers were in 2n= 38 to 40 and some abnormal phenomena such as chromosome bridge and lagged chromosomes in meiosis were observed, the pollens still could be normally formed without the influence on the normal seed setting. It was proved that the Ogu CMS restoring gene existed in CLR650 and the length of radish fragment was shorter than the Ogu CMS restoring line R113 by detecting the molecular markers which closely linked with radish restoring gene (Rf0). 【Conclusion】 The restoring line CLR650 could be a new restoring type for the Ogu CMS in B. napus.

Key words: Brassica napus, Raphanobrassica, grafting, wide hybridization, Ogu CMS restorer

陈卫江, 李莓, 王同华, 惠荣奎, 涂金星, 傅廷栋. 甘蓝型油菜萝卜细胞质雄性不育恢复材料的创制[J]. 中国农业科学, 2012, 45(8): 1465-1474.

CHEN Wei-Jiang, LI Mei, WANG Tong-Hua, HUI Rong-Kui, TU Jin-Xing, FU Ting-Dong. Creative Process of a New Restorer Material with Ogu CMS in Brassica nupus[J]. Scientia Agricultura Sinica, 2012, 45(8): 1465-1474.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2012.08.002

https://www.chinaagrisci.com/CN/Y2012/V45/I8/1465

参考文献

[1]Ogura H. Studies on the new male-sterility in Japanese radish, with special reference to the utilization of this sterility towards the practical raising of hybrid seeds. Memoirs of the Faculty of Agriculture, Kagoshima University, 1968, 6(2): 39-78.

[2]祝朋芳, 魏毓棠. Ogura胞质芸苔属作物雄性不育系的应用现状及前景. 长江蔬菜, 2004(4): 44-45.

Zhu P F, Wei Y T. Application and prospect of male sterility lines with Ogura cytoplasm in Brassica. Journal of Changjiang Vegetables, 2004(4): 44-45. (in Chinese)

[3]Bannerot H, Boulidard L, Cauderon Y, Tempe J. Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica oleracea. Proceedings of Eucarpia Meeting Cruciferae, 1974, 25: 52-54.

[4]Pelletier G, Primard C, Vedel F, Chetrit P, Remy R, Rousselle, Renard M. Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Molecular Genetics and Genomics, 1983, 191: 244-250.

[5]刘后利. 油菜的遗传和育种. 上海: 上海科学技术出版社, 1985: 387-391.

Liu H L. The Genetic and Breed of Rapeseed. Shanghai: Shanghai Scientific and Technical Press, 1985: 387-391. (in Chinese)

[6]Hiroshi Y. Distribution and allelism of restorer genes for Ogura cytoplasmic male sterility in wild and cultivated radishes. Genes Genetic Systems, 1998, 2(73): 79-83.

[7]Heyn F W. Transfer of restore genes from Raphanus to cytoplasmic male sterile Brassica napus. Cruciferae Newsletter, 1976, 1: 15-16.

[8]Pelletier G, Primard C, Vedel F, Chetrit P, Renard M, Pellan- Delourme R, Mesquida J. Molecular phenotypic and genetic characterization of mitochondrial recombinants in rapeseed. Proceedings of the 7th International Rapeseed Congress, Poznan, Pologne, 1987: 113-118.

[9]Pellan-Delourme R. Cytoplasmic male sterility in rapeseed (Brassica napus L.): Female fertility of restored with “Ogura” and cybrids cytoplasms. Genome, 1988, 30: 234-238.

[10]Delourme R, Bouchereau A, Hubert N, Renard M, Landry B S. Identification of RAPD markers linked to a fertility restorer gene for the Ogura radish cytoplasmic male sterility of rapeseed (Brassica napus L.). Theoretical and Applied Genetics, 1994, 88: 741-748.

[11]Delourme R, Eber F, Renard M. Breeding double low restorer lines in radish cytoplasmic male sterility of rapeseed (Brassica napus L.). Cambridge: Proceedings of the 9th International Rapeseed Congress, 1995, 1: 6-8.

[12]Delourme R, Horvais R, Valle P, Renard M. Double low restored F1 hybrids can be produced with the Ogu-INRA CMS in rapeseed. Canberra: Proceedings of the10th International Rapeseed Congress, 1999: 26-29.

[13]Bartkowiak-Broda I, Poplawska W. Characteristics of double low winter rapeseed lines with introduced restorer gene for CMS Ogura. Canberra: Proceedings of the 10th International Rapeseed Congress, 1999: 172.

[14]Pruvot J C, Raling K K, Charne D, Tulsieram L. Development of low glucosinolate restore and Ogura CMS winter rape hybrid. Canberra: Proceedings of the 10th International Rapeseed Congress, 1999, 270-273.

[15]Primard-Brisset C, Poupard J P, Horvais R, Eber F, Pelletier G, Renard M, Delourme R. A new recombined double low restorer line for the Ogu-INRA-CMS in rapeseed (Brassica napus L.). Theoretical and Applied Genetics, 2005(111): 736-746.

[16]李云昌, 蔡明, 郑元本，胡琼，刘贵华. 甘蓝型油菜萝卜质雄性不育系的研究及恢保关系测定. 中国油料, 1993(3): 8-11.

Li Y C, Cai M, Zheng Y B, Hu Q, Liu G H. Systematic study on Ogura CMS and test for restorer-maintenancer relationship in B.nupus. Chinese Journal of Oil Crop Sciences, 1993(3): 8-11. (in Chinese)

[17]魏忠芬, 王通强, 黄泽素, 何采萍. 甘蓝型油菜萝卜细胞质雄性不育研究进展. 贵州农业科学, 1999, 27(1): 101-105.

Wei Z F, Wang T Q, Huang Z S, He C P. Research progress of radish cytoplasmic male sterility of B. napus. Guizhou Agricultural Science, 1999, 27(1): 101-105. (in Chinese)

[18]文雁成, 张书芬, 王建平, 朱家成, 赵磊, 曹金华. 甘蓝型油菜萝卜质雄性不育恢复系的筛选和初步研究. 华北农学报, 2010, 25(4): 102-106.

Wen Y C, Zhang S F, Wang J P, Zhu J C, Zhao L, Cao J H. Screening and preliminary studies of restorers of radish cytoplasmic male sterile lines in Brassica napus L.. Acta Agriculturae Boreali-Sinica, 2010, 25(4): 102-106. (in Chinese)

[19]李琳, 李旭峰, 李红梅. 油菜萝卜质细胞雄性不育系恢复材料Ad-6(F5)的细胞遗传学研究. 四川大学学报:自然科学版, 1997, 34(6): 843-846.

Li L, Li X F, Li H M. Cytogenetical studies of the restoring line ad-6(F5) of rapeseed cytoplasmic male sterile line with Ogu CMS. Journal of Sichuan University: Natural Science Edition, 1997, 34(6): 843-846. (in Chinese)

[20]李旭峰, 李琳, 秦金红, 吴书惠, 袁旭, 王劲, 杨凡. 油菜萝卜质不育系测交后代恢复材料F2（TC1）的选育及细胞遗传学研究. 中国农业科学, 2001, 34(1): 108-110.

Li X F, Li L, Qin J H, Wu S H, Yuan X, Wang J, Yang F. Cytogenetic study on the restored material F2(TC1) from test cross progenies of B. napus with Ogura CMS. Scientia Agricultura Sinica, 2001, 34(1): 108-110. (in Chinese)

[21]Hu X Y, Sullivan-Gibert M, Kubit T, Danielson J, HnatiuK N, Marchione W, Greene T, Thompson S. Mapping of the Ogura fertility restorer gene Rfo and development of Rfo allele-specific markers in canola (Brassica napus L.). Molecular Breeding, 2008, 22: 663-674.

[22]胡大有, 王爱云. 甘蓝型油菜与萝卜属间杂种的获得及分子鉴定. 中国油料作物学报, 2006, 28(4): 476-479.

Hu D Y, Wang A Y. Production and molecular indentification of intergeneric hybrids between Brassica napus and Raphanus sativus. Chinese Journal of Oil Crop Sciences, 2006, 28(4): 476-479. (in Chinese)

[23]殷家明, 唐章林, 李加纳, 匡全. Ogura不育胞质对甘蓝型油菜与萝卜种间可交配性的影响. 中国农学通报, 2002, 18(1): 33-35.

Yin J M ,Tang Z L, Li J N, Kuang Q. Effect of Ogura sterile cytoplasm on interspecific crossability between Brassica napus and Raphanus sativus. Chinese Agricultural Science Bulletin, 2002, 18(1): 33-35.(in Chinese)

[24]Bannerot H, Boulidard L, Chueau Y. Unexpected difficulties met with the radish cytoplasm. Eucarpia Cruifeares News, 1997, l2: 16.

[25]Sakai T, Imamura J. Alteration of mitochondrial genomes containing atpA gene in the sexual progeny of cybrids between Raphanus sativus CMS line and Brassica napus cv. Westar. Theoretical and Applied Genetics, 1992, 84: 923-929.

[26]Iwabuchi M, Koizuka N, Fujimoto H, Sakai T, Imamura J. Identification and expression of the kosena radish (Raphanus sativus cv.Kosena) homologue of the Ogura radish CMS-associated gene, orf138. Plant Molecular Biology, 1999, 39(1): 183-188.