大额牛（Bos frontalis）与瘤牛（Bos indicus）的种间杂交研究

doi:10.3864/j.issn.0578-1752.2011.17.015

中国农业科学 ›› 2011, Vol. 44 ›› Issue (17): 3622-3633.doi: 10.3864/j.issn.0578-1752.2011.17.015

大额牛（Bos frontalis）与瘤牛（Bos indicus）的种间杂交研究

和占星, 张继才, 亐开兴, 金显栋, 王喆, 王安奎, 黄梅芬, 赵刚, 杨国荣, 涂先云, 袁希平, 黄必志

1. 云南省草地动物科学研究院
2. 西北农林科技大学动物科技学院
3. 云南省泸水县畜牧兽医局

收稿日期:2010-07-26 修回日期:2010-09-25 出版日期:2011-09-01 发布日期:2010-09-27
通讯作者: 通信作者袁希平，E-mail：yxp@ynbp.cn。通信作者黄必志，E-mail：hbz@ynbp.cn
作者简介:和占星，E-mail：hezx81@126.com。张继才，E-mail：ynzjc@126.com。和占星与张继才为同等贡献作者
基金资助:
云南省科技攻关与高新技术发展项目（2006NG23）、云南省自然科学基金项目（2006C0068M）、云南省科技创新人才培养项目（2010CI082）、云南省草地动物科学研究院院长基金（YNBP2006DF0002）、云南省农业科技创新工程项目（2008LA018）、国家现代农业（肉牛）产业技术体系（NYCYTX-38）

Study on the Interspecies Crossbreeding Between Mithun (Bos frontalis) and Zebu (Bos indicus) Cattle

HE Zhan-Xing, ZHANG Ji-Cai, XU Kai-Xing, JIN Xian-Dong, WANG Zhe, WANG An-Kui, HUANG Mei-Fen, ZHAO Gang, YANG Guo-Rong, TU Xian-Yun, YUAN Xi-Ping, HUANG Bi-Zhi

1. 云南省草地动物科学研究院
2. 西北农林科技大学动物科技学院
3. 云南省泸水县畜牧兽医局

Received:2010-07-26 Revised:2010-09-25 Online:2011-09-01 Published:2010-09-27

摘要/Abstract

摘要： 【目的】探讨大额牛（Bos frontalis）与瘤牛（Bos indicus）杂交利用潜能及杂交育种前景。【方法】选用婆罗门（Brahman）母牛，以CUE-MATETM（孕酮阴道栓）+ PG（氯前列烯醇）法同期发情处理后用大额牛冷冻精液对其进行人工授精（artificial insemination，AI），调查记录母牛产犊时间、妊娠期及犊牛性别等；测定大额牛与婆罗门牛杂交F1（大婆F1）、大额牛和婆罗门牛的初生体重及6、12和18月龄的体重；进行大婆F1的核型分析；对大婆F1的繁殖性能进行研究。【结果】 ① 婆罗门母牛的同期发情率、妊娠率和产犊率分别为83.87%、60.56%和45.07%，母牛（n=24）的平均妊娠期为（290.83±5.26）d；② 大婆F1中母犊占71.88%，分别显著高于大额牛和婆罗门牛（P＜0.05）、极显著高于大额牛与黄牛杂交F1（大黄F1）和BMY牛（P＜0.01）；③ 大婆F1（n=29）初生体重及6、12和18月龄平均体重分别为（26.69±4.84）、（153.08±37.58）、（219.34±31.31）和（302.06±28.92）kg，分别比大额牛（n=18）高68.18%、37.12%、45.07%和40.13%（P＜0.01）；分别比婆罗门（n=45）低14.40%（P＜0.01）、5.23%（P＞0.05）、13.12%（P＜0.01）和12.76%（P＜0.01）；0—18月龄大婆F1中公牛和母牛平均日增重分别为（503.09±44.91）g和（502.89±53.54）g，分别比大额牛高35.98%和39.85%（P＜0.01），但分别比婆罗门牛低16.49%（P＜0.01）和6.63%（P＞0.05）；④ 大额牛、婆罗门牛和大婆F1的染色体数目（2n）分别为58、60和59；大婆F1母牛（n=2）的初妊娠年龄、初妊娠体重和初产年龄分别为（16.00±2.83）月龄、（342.50±30.41）kg和（26.00±2.83）月龄，且年产1犊；⑤ 对5头、15—24月龄大婆F1公牛进行电刺激采精，均未采集到精液。【结论】大额牛和瘤牛杂交，其杂种牛生长发育快，适应性强，杂种优势明显；F1代母牛可繁育，且成熟早，繁殖力强；但F1代公牛是否可繁育有待进一步研究。

关键词: 大额牛, 婆罗门牛, 种间杂交, 生长性能, 杂种优势

Abstract: 【Objective】 The potential of interspecies crossbreeding utilization and its foreground between Mithun (Bos frontalis) and Zebu (Bos indicus) cattle were studied. 【Method】 Firstly, after treated by CUE-MATETM (progesterone-releasing intravaginal device) and PG for synchronization estrous, Brahman cows were inseminated artificially with Mithun’s frozen semen. And gestation length and calving time of cows and calf sex ratio were investigated. Besides, the body weight of Mithun and Brahman crossbreed at birth, 6 months, 12 months and 18 months of age were measured and karyotype and reproductive performance of hybrids F1 (Mithun×Brahman) and so on were analyzed and investigated. 【Result】 The rates of synchronization estrous, pregnancy and calving of Brahman cow were 83.87%, 60.56% and 45.07%, respectively. The average gestation length was (290.83±5.26) d (n=24). The percentage of female calf in crossbred F1 (Mithun×Brahman) was 71.88%，higher than those of Mithun and Brahman (P＜0.05), and significantly higher than those of crossbred F1 (Mithun×Yellow cattle) and BMY cattle, respectively (P＜0.01). The average body weight at birth, 6 months, 12 months and 18 months of crossbred F1 (Mithun×Brahman)(n=29) was (26.69±4.84) kg, (153.08±37.58) kg, (219.34±31.31) kg and (302.06±28.92) kg, respectively, which were 68.18%, 37.12%, 45.07% and 40.13% higher than those of Mithun (P＜0.01), but 14.40% (P＜0.01), 5.23% (P＞0.05), 13.12% (P＜0.01) and 12.76% (P＜0.01) lower than those of Brahman. The average daily gain (ADG) of crossbred F1 (Mithun×Brahman) from birth to 18 months of age was (503.09±44.91) g and (502.89±53.54) g for male and female，which were 35.98% and 39.85% higher than those of male and female of Mithun (P＜0.01), but 16.49% and 6.63% lower than those of male (P＜0.01) and female (P＞0.05) of Brahman. The chromosome numbers of Mithun, Brahman and their crossbred F1 (Mithun×Brahman) were 2n=58, 2n=60 and 2n=59, respectively. The average age and body weight of crossbred F1 (Mithun×Brahman) at first pregnancy and their primiparous age were (16.00±2.83) months, (342.50±30.41) kg and (26.00±2.83) months, respectively, which gave one calf per year. The semen collection of 5 crossbred F1 (Mithun×Brahman) at 15-24 months of age was failed by electro-ejaculation treatment.【Conclusion】Interspecies crossbreeding of Mithun×Zebu performed better in rapid growth, wide adaptation and significant heterosis. And the crossbred female F1 was of better reproductive capacity, precocity and high reproductive efficiency. However, the fertility of male crossbred F1 will remain to be studied in future.

Key words: Gayal or Mithun (Bos frontalis), Brahman (Bos indicus), Interspecies crossbreeding, growth performance, heterosis

和占星, 张继才, 亐开兴, 金显栋, 王喆, 王安奎, 黄梅芬, 赵刚, 杨国荣, 涂先云, 袁希平, 黄必志. 大额牛（Bos frontalis）与瘤牛（Bos indicus）的种间杂交研究[J]. 中国农业科学, 2011, 44(17): 3622-3633.

HE Zhan-Xing, ZHANG Ji-Cai, XU Kai-Xing, JIN Xian-Dong, WANG Zhe, WANG An-Kui, HUANG Mei-Fen, ZHAO Gang, YANG Guo-Rong, TU Xian-Yun, YUAN Xi-Ping, HUANG Bi-Zhi. Study on the Interspecies Crossbreeding Between Mithun (Bos frontalis) and Zebu (Bos indicus) Cattle[J]. Scientia Agricultura Sinica, 2011, 44(17): 3622-3633.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2011.17.015

https://www.chinaagrisci.com/CN/Y2011/V44/I17/3622

参考文献

[1]Bhusan S, Rajkhowa C, Bujarbaruah K M. Body weight and growth pattern in mithun (Bos frontalis). Indian Journal of Animal Sciences, 2005, 75(10): 1186-1188.

[2]兰宏, 熊习昆, 林世英, 刘爱华, 施立明. 云南黄牛和大额牛mtDNA多态性研究. 遗传学报, 1993, 20(5): 419-425.

Lan H, Xiong X K, Li S Y, Liu A H, Shi L M. Mitochondrial DNA polymorphism of cattle (Bos taurs) and mithun (Bos frontalis) in Yunnan province. Acta Genetica Sinica, 1993, 20(5): 419-425. (in Chinese)

[3]王兰萍, 耿荣庆, 常洪, 冀德君, 李永红, 常春芳. 大额牛起源与系统地位的遗传学分析. 云南农业大学学报: 自然科学版, 2009, 24(2): 231-234.

Wang L P, Geng R Q, Chang H, Ji D J, Li Y H, Chang C F. Genetic analysis on origin and phylogenetic status of Gayal (Bos frontalis). Journal of Yunnan Agricultural University: Natural Sciences, 2009, 24(2): 231-234. (in Chinese)

[4]苟潇, 王永奇, 杨舒黎, 邓卫东, 毛华明. 从mtDNA和SRY基因多态揭示云南大额牛杂交起源. 中国农业大学学报: 自然科学版, 2009, 14(1): 31-37.

Gou X, Wang Y Q, Yang S L, Deng W D, Mao H M. Hybrid origin of Yunnan Gayal revealed by mtDNA and SRY gene sequence variation. Journal of China Agricultural University: Natural Sciences, 2009, 14(1): 31-37. (in Chinese)

[5]冀德君, 常春芳, 常洪, 耿荣庆, 李永红. 中国牛亚科6个物种MSTN基因外显子2多态性及分化研究. 中国畜牧杂志, 2009(45): 1-3.

Ji D J, Chang C F, Chang H, Geng R Q, Li Y H. Polymorphism and phylogenetic relationship of myostatin gene exon 2 in 6 bovinae species in China. Chinese Journal of Animal Science, 2009(45): 1-3. (in Chinese)

[6]单祥年, 陈宜峰, 罗丽华, 曹筱梅, 宋继志, 曾养志. 中国黄牛属(Bos)五个种的染色体比较研究. 动物学研究, 1980, 1(1): 75-81.

Shan X N, Chen Y F, Luo L H, Cao X M, Song J Z, Zeng Y Z. Comparative studies on the chromosomes of five species of cattle of the Bos in China. Zoological Research, 1980, 1(1): 75-81. (in Chinese)

[7]Ma G L, Chang H, Li S P, Chen H Y, Ji D J, Geng R Q, Chang C F, Li Y H. Phylogenetic relationships and status quo of colonies for Gayal based on analysis of cytochrome b gene partial sequences. Journal of Genetics and Genomics, 2007, 34(5): 413-419.

[8]云南省家畜家禽品种志编写委员会. 云南省家畜家禽品种志. 昆明: 云南科技出版社, 1987,.

Editorial Committee of the “Breeds of Domestic Animal and Poultry” in Yunnan. Breeds of Domestic Animal and Poultry in Yunnan. Kunming: Yunnan Science and Technology Press, 1987.

[9]Heli T, Saikaia S, Bora N N. MITHUN: the magnificient bos species of North-East India：its physical characteristics. Indian Journal of Animal, Production, Management, 1996, 12(3, 4): 144-153.

[10]Mondal S K, Bujarbaruah K M, Khaie K, Borah I P. Body conformation traits in mithun (Bos frontalis). Indian Journal of Animal Sciences, 2004, 74(1): 84-87.

[11]和占星, 亐开兴, 袁希平, 桑业华, 马文张, 张继才, 李子良. 凤凰山迁地保种大额牛的外貌特征及主要习性. 云南农业大学学报: 自然科学版, 2009, 24(2): 225-230.

He Z X, Qu K X, Yuan X P, Sang Y H, Ma W Z, Zhang J C, Li Z L. Appearance character and major behavior of gayal (Bos frontalis) on the conservation ex situ in Phenix Mountain. Journal of Yunnan Agricultural University: Natural Sciences, 2009, 24(2): 225-230. (in Chinese)

[12]王永奇, 苟潇, 文际坤, 毛华明, 苏八七, 张勇. 独龙牛生长发育规律的研究. 云南农业大学学报: 自然科学版, 2010, 25(2): 63-68.

Wang Y Q, Gou X, Wen J K, Mao H M, Su B Q, Zhang Y. Research on growth and development of Dulong Cattle. Journal of Yunnan Agricultural University: Natural Sciences, 2010, 25(2): 63-68. (in Chinese)

[13]葛长荣, 陈韬, 袁希平. 大额牛、云南瘤牛屠宰性能研究. 黄牛杂志, 1996, 22(3): 31-33.

Ge C R, Chen T, Yuan X P. A study on slaughtering performance of Yunnan Zebu and width forehead cattle (Bos frontalis). Journal of Yellow Cattle Science, 1996, 22(3): 31-33. (in Chinese)

[14]葛长荣, 田允波. 大额牛、云南瘤牛横纹肌组织形态学研究. 西南民族学院学报: 自然科学版, 1996, 22(1): 33-36.

Ge C R, Tian Y B. Studies on the striated muscles of the the Gayal and the Zebu. Journal of Southwest Nationalities College: Natural Sciences, 1996, 22(1): 33-36. (in Chinese)

[15]Giasuddin M, Huque K S, Alam J. Reproductive potentials of mithun (Bos frontalis) under semi-intensive management. Asian-Australasian Journal of Animal Sciences, 2003, 16(3): 331-334.

[16]Dhali A, Mishra D P, Karunakaran M, Mech A, Rajkhowa C. Secretion patterns of luteinising hormone, follicle-stimulating hormone and 17β-oestradiol during oestrus and the mid-luteal phase of the oestrous cycle in mithun (Bos frontalis). Reproduction, Fertility and Development, 2006, 18: 619-626.

[17]He Z X, Yuan X P, Qu K X, Huang B Z, Ma W Z, San Y H, Li Z L, Yang S H. Investigation of calving behavior of Gayal (Bos frontalis) under semi-wild in China. Reproduction in Domestic Animals, 2008, 43(Suppl.3): 217-218.

[18]Yang S L, Ma S C, Chen J, Mao H M, He Y D, Xi D M, Yang L Y, He T B, Deng W D. Bacterial diversity in the rumen of Gayal (Bos frontalis), swamp buffaloes (Bubalus bubalis) and holstein cow as revealed by cloned 16S rRNA gene sequences. Molecular Biology Reports, 2010, 37: 2063-2073.

[19]和协超, 沈放, 季维智, 赵家才, 刘国璋, 杨国荣, 和占星. 电刺激采集大额牛精液及其低温冷冻的初步研究. 兽类学报, 2000, 20(3): 232, 239-240.

He X C, Shen F, Ji W Z, Zhao J C, Liu G Z, Yang G R, He Z X. Successful electroe jaculation and semen cryopreservation of Bos frontalis. Acta Theriologica Sinica, 2000, 20(3): 232, 239-240. (in Chinese)

[20]Mondal M, Karunakaran M, Lee K B, Rajkhowa C. Characterization of Mithun (Bos frontalis) ejaculates and fertility of cryopreserved sperm. Animal Reproduction Science, 2010, 118: 210-216.

[21]Dhali A, Karunakaran M, Mech A, Nath N, Prakash B, Rajkhowa C, Mishra D P. Birth of the first Mithun (Bos frontalis) calf through artificial insemination. Animal, 2008, 2(6): 879-882.

[22]Winter H, Mayr B, Schleger W, Dworak E, Krutzler J. Genetic characterisation of the mithun (Bos frontalis) and studies of spermatogenesis, blood groups and haemoglobins of its hybrids with Bos indicus. Research in Veterinary Science, 1986, 40(1): 8-17.

[23]Huque K S, Rahman M M, Jalil M A. Study on the growth pattern of mithuns (Bos frontalis) and their crossbred calves. Asian-Australasian Journal of Animal Sciences, 2001, 14(9): 1245-1249.

[24]范江平, 叶绍辉, 葛长荣, 和天宝, 黄玉路. 杂交大额牛肉质特性研究初报. 云南农业大学学报: 自然科学版, 2005, 20(4): 600-602.

Fan J P, Ye S H, Ge C R, He T B, Huang Y L. Research on the meat characteristic of crossbred mithun. Journal of Yunnan Agricultural University: Natural Sciences, 2005, 20(4): 600-602. (in Chinese)

[25]和占星, 张继才, 亐开兴, 黄必志, 袁希平, 赵刚, 黄梅芬, 张勇, 何永珍. 大额牛冻精在瘤牛超排中的应用效果及F1代的亲子鉴定. 中国农业科学, 2008, 41(8): 2436-2441.

He Z X, Zhang J C, Qu K X, Huang B Z, Yuan X P, Zhao G, Huang M F, Zhang Y, He Y Z. Application of frozen sperm of Mithun in superovulation of Zebu and parentage identification of crossbred F1(Mithun×Brahman). Scientia Agricultura Sinica, 2008, 41(8): 2436-2441. (in Chinese)

[26]He Z X, Suzuki T, He X C, Zhan J C, Huang B Z. Seasonal effect of superovulation for beef cattle at the subtropical grassland//Agriculture and Animal Husbandry College of Qinghai University, ed. The 13th International Congress on Biotechnology in Animal Reproduction. Xining: Qinghai People’s Publishing House, 2006.

[27]Messine O, Schwalbach L J M, Mbah D A, Ebangi A L. Non-genetic factors affecting gestation length and postpartum intervals in gudali Zebu cattle of the Adamawa Highlands of Cameroon. Tropicultura, 2007, 25(3): 129-133.

[28]Torell R. Gestation length of beef cows could signal dystocia problems. www.Western Farmer Stockman. Com October 2009: 32.

[29]李永海, 段志远, 孟宪印. 精氨酸对奶牛受胎率及后代性别的影响. 中国兽医科技, 1994, 24(2): 27-28.

Li Y H, Duan Z Y, Meng X Y. Effect of arginine on offspring sex of milk cow. China Veterinary Medicine Technology, 1994, 24(2): 27-28. (in Chinese)

[30]Gutiérrez-Adán A J, Granados B, Pintado, de La Fuente J. Influence of glucose on the sex ratio of bovine IVM/IVF embryos cultured in vitro. Reprodction, Fertility and Development, 2001, 13(5-6): 361-365.

[31]Lechniak D, Strabel T, Bousquet D, King A W. Sperm pre-incubation prior to insemination affects the sex ratio of bovine embryos produced in vitro. Reproduction in Domestic Animals, 2003, 38(3): 224-227.

[32]James W H. Evidence that mammalian sex ratios at birth are partially controlled by parental hormone levels at the time of conception. Journal of Theoretical Biology, 1996, 180(4): 271-286.

[33]郭爱朴. 牦牛、黄牛及其杂交后代犏牛的染色体比较研究. 遗传学报, 1983, 10(2): 137-143.

Guo A P. A comparative study on chromosomes of oxen (Bos taurus), yaks (Bos grunnieus) and their hybrids. Acta Genetica Sinica, 1983, 10(2): 137-143. (in Chinese)

[34]张容昶, 段李成. 对牦牛和普通牛种间杂种公牛精子生成水平的研究. 畜牧兽医学报, 1991, 22(3): 231-234.

Zhang R C, Duan L C. A study on the sperm productivity of species hybrid bull between yaks and cattle. Acta Veterinaria et Zootechnica Sinica, 1991, 22(3): 231-234. (in Chinese)

[35]董丽艳, 李齐发, 屈旭光, 李隐侠, 李新福, 徐洪涛, 谢庄. 黄牛、牦牛和犏牛睾丸组织中Cdc2、Cdc25A基因mRNA表达水平. 遗传, 2009, 31(5): 495-499.

Dong L Y, Li Q F, Qu X G, Li Y X, Li X F, Xu H T, Xie Z. Expression levels of Cdc2 and Cdc25A mRNA in cattle, yak, and cattle-yak testis. Hereditas, 2009, 31(5): 495-499. (in Chinese)

[36]黄右军, 尚江华, 梁梦玫, 张秀芳, 黄芬香. 河流型水牛与沼泽型杂交后代(2n=49)染色体遗传与繁殖力的研究. 遗传, 2003, 25(2): 155-159.

Huang Y J, Shang J H, Liang M M, Zhang X F, Huang F X. Studies of chromosomal heredity and fertility of progenies (2n=49) crossed between river and swamp buffalo. Hereditas, 2003, 25(2): 155-159. (in Chinese)

[37]Vadhanakul N, Tunwattana W, Sekkasiddhi P, Tansatit M, Chavananikul V. Karyotype of crossbred progeny born to the interspecies hybridization of wild and zebu cattle with reference to their fertility and growth performance. Thai Journal Veterinary, Medicine, 2004, 34(1): 73-81.

[38]赵志军, 赵刚, 余梅, 金显栋, 黄必志. 云南引进婆罗门牛纯种繁育效果研究. 中国畜牧杂志, 2009(增刊): 193-196.

ZhaoZ J, Zhao G, Yu M, Jin X D, Huang B Z. Study on effect of pure breed on introduce into Brahman in Yunnan. Chinese Journal of Animal Science, 2009(Suppl.): 193-196. (in Chinese)

[39]Newman S, Deland M P. Lifetime productivity of crossbred cows 2. Age and weight at first oestrus, calf birth weight, assisted calvings, calving interval and reproduction rate. Australian Journal of Experimental Agriculture, 1991, 31: 293-300.

[40]Beate D Scherf. World Watch List for Domestic Animal Diversity. 3rd edn. Rome: Food and Agriculture Organization of the Untied Nations, 2000, 8-584.

[41]任建华. “抗旱王”肉用牛简介. 浙江畜牧兽医, 1981, 1: 19.

Ren J H. Brief introduction in “drought master” beef cattle. Zhejiang Journal of Animal Science and Veterinary, 1981, 1: 19. (in Chinese)

[42]O’Connor S F, Tatum J D, Wulf D M, Green R D, Smith G C. Genetic effects on beef tenderness in Bos indicus composite and Bod taurus cattle. Journal of Animal Science, 1997, 75(7): 1822-1830.

大额牛（Bos frontalis）与瘤牛（Bos indicus）的种间杂交研究

Study on the Interspecies Crossbreeding Between Mithun (Bos frontalis) and Zebu (Bos indicus) Cattle

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	谢伶俐,韦丁一,章子爽,徐劲松,张学昆,许本波. 甘蓝型油菜发育进程中赤霉素动态变化及其与产量的关系[J]. 中国农业科学, 2022, 55(24): 4793-4807.
[2]	车大璐,赵俐辰,程素彩,刘爱瑜,李晓宇,赵寿培,王健诚,王媛,高玉红,孙新胜. 垫料床对育肥羔羊生长性能和臭气排放的影响[J]. 中国农业科学, 2022, 55(24): 4943-4956.
[3]	琚铭, 苗红梅, 黄盈盈, 马琴, 王慧丽, 王翠英, 段迎辉, 韩秀花, 张海洋. 芝麻种间杂交亲和性差异及杂种生物学特征分析[J]. 中国农业科学, 2022, 55(20): 3897-3909.
[4]	陈志敏,常文环,郑爱娟,蔡辉益,刘国华. 饲粮中添加膨化羽毛粉对肉鸡生长性能、屠宰性能和血清生化指标的影响[J]. 中国农业科学, 2022, 55(13): 2643-2653.
[5]	王金飞,杨国义,樊子菡,刘旗,张鹏程,任有蛇,杨春合,张春香. 饲粮中全株玉米青贮比例对杜湖杂交母羔生长性能、瘤胃发酵、养分消化率及血清学指标的影响[J]. 中国农业科学, 2021, 54(4): 831-844.
[6]	李姜玲,杨澜,阮仁武,李中安. 杂交小麦苗期光合特性分析及其对强优势组合的早期预测[J]. 中国农业科学, 2021, 54(23): 4996-5007.
[7]	张兰,王良治,黄艳玲,廖秀冬,张丽阳,吕林,罗绪刚. 饲粮微量元素添加模式对肉仔鸡生长和胴体性能及肌肉品质的影响[J]. 中国农业科学, 2021, 54(22): 4906-4916.
[8]	刘娇,陈志敏,郑爱娟,刘国华,蔡辉益,常文环. 葡萄糖氧化酶对大肠杆菌攻毒肉鸭生长性能、免疫功能及肠道健康的影响[J]. 中国农业科学, 2021, 54(22): 4917-4930.
[9]	王晨,张宏伟,王虎成,孙晓萍,李发弟,杨博辉. 高山美利奴育成羊的能量与蛋白质需要量[J]. 中国农业科学, 2021, 54(16): 3537-3548.
[10]	黄文琴,吕小康,庄一民,崔凯,王世琴,刁其玉,张乃锋. 早期断奶和育肥期饲粮NDF水平对湖羊生长性能和消化代谢的影响[J]. 中国农业科学, 2021, 54(10): 2217-2228.
[11]	杨云燕,王其炎,彭地纬,潘一帆,高晓梅,宣泽义,陈少梅,邹彩霞,曹艳红,林波. 日粮添加肉桂醛对奶牛公犊生长、健康及瘤胃发酵性能的影响[J]. 中国农业科学, 2021, 54(10): 2229-2238.
[12]	赵久然, 李春辉, 宋伟, 刘新香, 王元东, 张如养, 王继东, 孙轩, 王夏青. 玉米骨干自交系京2416杂种优势及遗传重组解析[J]. 中国农业科学, 2020, 53(22): 4527-4536.
[13]	李永祥,李春辉,杨俊品,杨华,程伟东,汪黎明,李凤艳,李会勇,王延波,李淑华,扈光辉,刘成,黎裕,王天宇. 中国玉米骨干亲本黄早四杂种优势形成的遗传基础解析[J]. 中国农业科学, 2020, 53(20): 4113-4126.
[14]	孔凡林,李媛,唐梦琪,马满鹏,付彤,刁其玉,成思源,屠焰. 氨基酸缺乏对母犊牛生长和日粮养分消化代谢的影响[J]. 中国农业科学, 2020, 53(2): 418-430.
[15]	任春燕,毕研亮,郭艳丽,杜汉昌,于博,屠焰,刁其玉. 开食料中性洗涤纤维水平对犊牛生长性能、血清生化指标和抗氧化功能的影响[J]. 中国农业科学, 2020, 53(2): 440-450.