关键词: MbNramp1, 铁, DDY4, 亚细胞定位

Abstract:

【Objective】 The objective of the study is to investigate the function of MbNramp1 gene. 【Method】 Heterogenous complementation experiments were employed to confirm the iron- transporting ability of MbNramp1,and study subcellular localization of the MbNRAMP1 protein 【Result】 MbNramp1 was expressed in yeast mutant DDY4, which could restore growth in the media without iron. At tested concentrations of BPDS, the yeast cells DDY4 mutant after MbNramp1 being transformed grew better than mutant strain transformed with empty vector. When the concentration of BPDS in the media reached 15 μmol?L-1, the growth of yeast cells with MbNramp1 was comparable with that of wild type. Although the growth of MbNramp1 transformed cells in the media with 30 μmol?L-1 BPDS was slower than those in the media with low concentration of BPDS, they were significantly faster that DDY4 mutant transformed with empty vector. In addition, wild type grew slower with 30 μmol?L-1 BPDS. The result of subcellular localization showed that MbNRAMP1 proteins were mainly positioned in the part of plasma membrane not in the whole membrane. 【Conclusion】 Initial results suggested that MbNRAMP1 protein has the ability of transferring Fe and also help yeast mutant to grow. In addition, MbNRAMP1 proteins were mainly positioned in the part of plasma membrane

Key words: MbNramp1, iron, DDY4, subcellular localization