关键词: 小麦糖原合成酶激酶, 绿色荧光蛋白, 拟南芥, 盐胁迫, 抗渗透

Abstract: 【Objective】The localization and function of wheat glycogen synthase kinase (TaGSK1) in plant cells was studied. 【Method】Tagsk1 gene linked with gfp gene was transformed into Arabidopsis via Agrobactiria. Arabidopsis containing gfp was used as control, the distribution of TaGSK1 in root cells was observed with laser confocal microscope. Different transgenic plants were planted in MS medium containing different concentrations of NaCl for screening salt-tolerant plants. Using the wild type Arabidopsis as control, the length of taproots was measured on the seventh day and the lateral roots number was counted on the ninth day. 【Result】 It was found that the green fluorescence of fused protein TaGSK-GFP distributed in cytoplasm of the transgenic plant cells, and mainly in root meristem and stelar sheath, where new roots would develop. Significant difference (P<0.01) existed between transgenic plants and their control plants about the root number and length. 【Conclusion】It is suggested that TaGSK1 localized in cytoplasm and could improve cell proliferation, development, anti-osmotic and anti-stress ability of transgenic plants.

Key words: TaGSK1, GFP, Arabidopsis, Salt tolerance, Anti-osmotic