中国农业科学 ›› 2006, Vol. 39 ›› Issue (12): 2483-2490 .

• 植物保护 • 上一篇    下一篇

棉花根部损伤程度对感染黄萎病菌后体内抗性酶的影响

范尉尉,马春红,董文琦,李运朝,刘子会,贾银锁,耿军义,张香云   

  1. 河北省农林科学院遗传生理研究所
  • 收稿日期:2006-04-25 修回日期:1900-01-01 出版日期:2006-12-10 发布日期:2006-12-10
  • 通讯作者: 马春红

The Cotton Root Hurts Degree to the Verticillium dahliae Kleb Infection of Behind the Influence of Anti-enzyme Inside the Body

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  1. 河北省农林科学院遗传生理研究所
  • Received:2006-04-25 Revised:1900-01-01 Online:2006-12-10 Published:2006-12-10

摘要: 【目的】探明棉花黄萎病对不同伤根程度棉苗体内相关抗性酶的影响。【方法】棉苗长至四叶时,将纸钵与蛭石等去掉,用水小心冲刷,得到:⑴完整根苗。⑵将根下部3~5 cm的余根用消毒的刀切掉为切根苗。⑶留主根,将大部分侧根切除为伤根苗。3种不同根系状况的棉苗浸于20 ml棉花黄萎病菌的菌液中,以无菌水稀释至不同浓度。经0~48 h,记载致萎蔫程度,并测定相关抗性酶的变化。【结果】3种伤根处理的棉苗浸黄萎病菌培养液后,其致萎度差异显著。当病菌液的浓度为1﹕10时,整根苗不出现感病,受害最轻;其次为伤根苗,2级感病;切根苗最为严重,3级感病。同时还测定了与提高抗病性有关酶的变化,以1﹕50浓度处理效果最好,过氧化物酶(POD)活性伤根苗达42.96 U mg-1•min-1,整根苗达到较高值49.2 U mg-1•min-1,切根棉苗为38.2U mg-1•min-1。有害物质丙二醛(MDA)的含量切根棉苗为39.483 mmol•g-1,伤根苗为27.12 mmol•g-1,整根苗为最低值3.845 mmol•g-1。而体内相关抗性酶如过氧化物酶(POD)活性的高低与有害物质丙二醛(MDA)含量的多少,其顺序与受害轻重的顺序一致。【结论】棉花伤根后的棉苗外部病理反应与体内相关酶的动态生化反应进行研究,获得植株外部病理反应与内部生化实验相吻合的结果。

关键词: 棉花, 黄萎病菌, 根部损伤, 过氧化物酶, 超氧化物歧化酶, 丙二醛

Abstract: 【Objective】Explore the influence that the clear cotton Verticillium dahliae harms a root related anti- enzyme in the degree cotton seedling body to the dissimilarity.【Method】Threw away the paper pot and vermiculite etc. when the cotton seedling was long to go to four leaves, use a water to flush carefully, get:⑴ Complete root seedling, ⑵Root the remaining root of the lower part 3-5 cm use to disinfect of the knife slice in order to slice a seedling, ⑶ Stays a main root, cutting off the greatly parts of side root for harm a seedling. Three kinds of different roots cotton seedling that fasten condition immerses to go to by having no germ water dilution a different density in the germ liquid of the 20 ml cotton Verticillium dahliae kleb. Through the 0-48 h with the result that the wilt degree and measures the variety of related anti-enzyme【Result】Slice a seedling victimizes most seriously and secondly in order to harm a seedling, complete root the outbreak that fasten the cotton seedling is the most light.【Conclusion】Result harmed the cotton seedling exterior pathology in root reaction and the dynamic state bio-chemical reaction of the related enzyme inside the body to carry on a research for the cotton, acquired the plant exterior pathology to respond to test with internal bio-chemical to fit together mutually.

Key words: CottonVerticillium dahliae Klebinjure of rootPOD, SOD, MDA