中国农业科学 ›› 2018, Vol. 51 ›› Issue (6): 1167-1181.doi: 10.3864/j.issn.0578-1752.2018.06.015
郭仰东,张磊,李双桃,曹芸运,齐传东,王晋芳
收稿日期:
2017-07-21
出版日期:
2018-03-16
发布日期:
2018-03-16
作者简介:
郭仰东,Tel:010-62734845;E-mail:yaguo@cau.edu.cn
基金资助:
GUO YangDong, ZHANG Lei, LI ShuangTao, CAO YunYun, QI ChuanDong, WANG JinFang
Received:
2017-07-21
Online:
2018-03-16
Published:
2018-03-16
摘要: 蔬菜作为重要的经济作物,近年来的种植面积、产量及需求都在不断增加。蔬菜作物在生长和发育过程中经常受到非生物逆境(包括干旱、盐、极端温度及重金属胁迫等)的侵害,影响其产量及品质。近十年来,国内外关于蔬菜应答非生物逆境胁迫的分子生物学研究领域取得了一定的进展。在应答干旱胁胁迫方面,DREB、WRKY、NAC、bHLH及bZIP等转录因子受干旱信号诱导,调节下游抗旱基因的表达,从而提高蔬菜作物抗旱能力。同时,水分运输相关功能基因(PIP、TIP)、E3连接酶SIZ1及脱水蛋白DHN也被报道受干旱诱导,并通过调节水势、渗透势及ROS积累抵御干旱胁迫。在抵御盐胁迫方面,SOS途径至关重要。SlSOS2能够通过调节SlSOS1和Na+/H+逆向转运蛋白LeNHX2/4的表达维持离子平衡和调节植物器官中Na+的分配。蔬菜抗盐研究中NAC、ERF、MYB等转录因子响应盐胁迫并激活抗逆相关基因表达,从而提高蔬菜作物抗盐能力。此外蔬菜植物大量合成渗透调节物质是其抵御盐胁迫的常见方式。吡咯啉-5-羧酸合成酶PvP5CS和tomPRO2、脯氨酸脱氢酶BoiProDH等在盐胁迫下能提高脯氨酸的含量;过表达甜菜碱醛脱氢酶SlBADH能提高番茄中甜菜碱含量。在高温胁迫响应过程中,HSFs位于调控网络的核心位置,可调控包括HSPs在内的一系列抗逆基因的表达,番茄中热激转录因子SlHSFs相互之间形成复合体调控下游SlHSPs的表达而应答高温逆境。在低温胁迫中,CBFs/EREBs位于调控网络的核心位置,并受ICE1调控;LEA及HSPs蛋白在低温下能够防止细胞中蛋白质变性并维持细胞膜流动性。蔬菜应答重金属胁迫主要依靠体内隔离和体内外螯合机制。在蔬菜应答非生物逆境的过程中,ABA作为信号受体起到至关重要的作用。蔬菜中NAC、MYB、HSF等转录因子则受ABA信号诱导,应答非生物逆境,进而提高活性氧清除能力,合成更多抗逆物质,从而抵御非生物逆境的侵害。
郭仰东,张磊,李双桃,曹芸运,齐传东,王晋芳. 蔬菜作物应答非生物逆境胁迫的分子生物学研究进展[J]. 中国农业科学, 2018, 51(6): 1167-1181.
GUO YangDong, ZHANG Lei, LI ShuangTao, CAO YunYun, QI ChuanDong, WANG JinFang. Progresses in Research on Molecular Biology of Abiotic Stress Responses in Vegetable Crops[J]. Scientia Agricultura Sinica, 2018, 51(6): 1167-1181.
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