关键词: 杏, 自交不亲和, 花柱, 蛋白质组

Abstract:

【Objective】 The objective of the experiment is to explore self-incompatibility (SI) molecular mechanism of apricot at proteome level.【Method】Different proteomics of style at the stage of the small bud, big bud, 24 h after self-pollination (ASP) and 24 h after cross-pollination (ACP) (‘Xinshiji’בBadanshui’) in the SI apricot ‘Xinshiji’ by two dimension difference gel electrophoresis (2D-DIGE) and mass spectrum (MS). 【Result】About 1500 protein points were detected, and 66 different proteins were expressed at four different development stage of style in ‘Xinshiji’. Self and non-self pollen had triggered different effects on style at proteomic level. There was one protein expressed special in cross-pollinated style, and four up-expressed and twenty-nine down-expressed in cross-pollinated style, respectively, compared with self-pollinated style. Thirteen protein spots were identified unambiguously.【Conclusion】 Different reactions on style at proteomic level had been obviously triggered in ‘Xinshiji’ by self and non-self pollen.

Key words: apricot, self-incompatiblility, style, proteome