关键词: 细胞骨架, 微丝解聚剂, 非寄主, 小麦, 过敏性坏死反应, H2O2

Abstract:

【Objective】The roles of microfilaments in the non-host resistance of wheat were elucidated in the study.【Method】Hypersensitive response (HR), H2O2 and papilla formation of the wheat leaves treated with actin polymerization inhibitor before inoculation of Sphaerotheca fuliginea conidia were investigated in the non-host interaction of wheat-S. fuliginea through trypan-blue, DAB, and coomassie brilliant blue staining, respectively. 【Result】 The results showed that the conidial germination of S. fuliginea was significantly delayed on the wheat, and the majority of the appressoria were malformed. Treated with cytochalasin A, the production of HR and H2O2, and the papilla formation on wheat leaves were severely suppressed and the incidences of them were markedly reduced, but the penetration incidence of S. fuliginea conidia was greatly increased and the conidia were able to form haustoria, even to form secondary hyphae occasionally. 【Conclusion】 It was concluded that the microfilaments are concerned with the non-host resistance response of wheat, and the polymerization of the microfilaments is essential to the resistance response.

Key words: cytoskeleton, cytochalasin, non-host, wheat, hypersensitive response, H2O2