关键词: 茶树, 愈伤组织, 儿茶素, 蛋白质组学

Abstract:

【Objective】 To establish a two-dimensional electrophoresis (2-DE) of proteome suitable for tea callus and analyze the differential protein expression patterns of tea callus with different catechins contents, which could provide a basis for the further study of proteomics of the catechins biosynthesis. 【Method】 Using the improved protein extraction, 2-DE and LTQ-ESI-MS/MS method, the differential protein expression profiling was investigated using the tea callus of “Yunjing63X” (with low catechins contents), “Yunjing63Y” (with higher contents), and “Yunjing63Y” cultured under light (with the highest contents). 【Result】 Protein quantitative and SDS-PAGE electrophoresis results showed that TCA-acetone method was the best for extracting tea callus proteins. And 14 differential protein spots were identified by 2-DE electrophoresis, LTQ-ESI-MS/MS and database searching. The glutathione S-transferase, WD40 family protein, catechol O-methyltransferase, S-adenosylmethionine synthetase, pectin methylesterase and so on were identified among those differential protein spots. 【Conclusion】 The predicted function of these picked proteins were involved in many physiological reactions, such as phenylpropanoid and flavonoids biosynthesis, transport and regulation, ethylene biosynthesis, cell wall metabolism , glycolysis pathway, and signal transduction.

Key words: tea plant, callus, catechins, proteomics