中国农业科学 ›› 2007, Vol. 40 ›› Issue (8): 1738-1745 .

• 园艺 • 上一篇    下一篇

利用CAPS标记转育野生番茄的高产基因

李 红,杜永臣,王孝宣,宋 明,高建昌,国艳梅,朱德蔚,戴善书   

  1. 西南大学园艺园林学院
  • 收稿日期:2006-02-27 修回日期:2006-05-31 出版日期:2007-08-10 发布日期:2007-08-10
  • 通讯作者: 杜永臣

High Yield Gene was Introgressed from L.hirsutum acc.LA1777 by CAPS Marker

  

  1. 西南大学园艺园林学院
  • Received:2006-02-27 Revised:2006-05-31 Online:2007-08-10 Published:2007-08-10

摘要: 【目的】将野生多毛番茄中的高产基因渗入到普通番茄骨干亲本以创造高产番茄育种材料。【方法】TA1229是野生多毛番茄(Lycopersicon hirsutum acc.)LA1777的一个近等基因系,带有来源于LA1777的高产基因(QTL)。通过杂交、回交和分子标记辅助选择方法对TA1229×9706的BC1群体进行遗传分析。【结果】获得23个比TA1229含有的外源染色体片段更短的渐渗系,其中16个渐渗系为高产番茄材料,并检测到了一个影响番茄成熟果实平均重量的QTL,将其定位于标记TG53和TG158之间。【结论】16份渐参系可用作为高产番茄新种质。

关键词: CAPS, QTL, 分子标记辅助选择, 高产, 番茄

Abstract: TA1229 is a near-isogenic line in the Lycopersicon esculentum cv E6203 background and contains a 24-cM introgression at the bottom of chromosome 1 from L.hirsutum acc.LA1777. TA1229 has high yield genes. By the means of the hybridization and backcross with the elite tomato material-9706, a set of sub-NILs which have high yield trait derived from TA1229 were developed. And the fragment length of these sub-NILs was shorter than 24-cM. Through yield analysis ,16 high yield tomato materials were obtained and QTL affecting yield was located between TG53 and TG158.

Key words: CAPS, QTL, Marker assisted selection, High yield, Tomato