中国农业科学 ›› 2007, Vol. 40 ›› Issue (7): 1528-1534 .

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

鸵鸟源隐孢子虫的种类鉴定及其生物学特性研究

孙铭飞,张龙现,宁长申,梁宏德,菅复春,张 敏   

  1. 河南农业大学牧医工程学院
  • 收稿日期:2005-12-09 修回日期:2007-02-09 出版日期:2007-07-10 发布日期:2007-07-10
  • 通讯作者: 张龙现

The First Record of Cryptosporidium from Ostrich in China and

Longxian Zhang   

  1. 河南农业大学牧医工程学院
  • Received:2005-12-09 Revised:2007-02-09 Online:2007-07-10 Published:2007-07-10
  • Contact: Longxian Zhang

摘要: 【目的】掌握鸵鸟隐孢子虫病的流行范围和流行动态,以及研究鸵鸟源隐孢子虫的生物学特性。【方法】应用饱和蔗糖溶液漂浮法检查鸵鸟新鲜粪样404份,详细观察、测量卵囊形态大小;收集纯化阳性样品中的隐孢子虫卵囊,分别人工经口感染幼鸵鸟、雏鸡和小白鼠并利用组织病理学试验技术研究其生物学特性。【结果】检测到阳性样品10份,总阳性率为2.48% (10/404),且感染率与鸵鸟日龄有一定的关系。其卵囊平均大小为5.43×4.38μm(4.0~6.0×3.7~5.0μm),形状指数1.24(长/宽),(n=50)。分离到的卵囊能成功感染雏鸡和幼鸵鸟,但不能感染小白鼠。受试雏鸡具有明显的呼吸道症状,而幼龄鸵鸟呼吸道症状较轻微。此外,在两种动物体内的排卵囊规律差别较大,在鸵鸟体内出现多个排卵囊高峰。虫体主要寄生于雏鸡和幼鸵鸟的泄殖腔和法氏囊,引起法氏囊萎缩、微绒毛大量脱落等病理变化。【结论】根据其卵囊形态、宿主范围和虫体寄生部位等生物学特性,结合文献报道,笔者在国内发现并初步鉴定出鸵鸟源隐孢子虫为贝氏隐孢子虫(Cryptosporidium baileyi)。

关键词: 鸵鸟, 隐孢子虫, 交叉感染, 电镜, 种类鉴定

Abstract: A survey of Cryptosporidium was performed in 404 samples from ostriches in ZhengZhou, with the Sheather's sugar flotation technique.The cryptosporidia infection percentage in ostriches was 2.48%(10/404), the oocyst is of 5.43μm×4.38μm with shape index of 1.24(n=50). The pathogenicity of the Cryptosporidium strain to ostriches,chickens and mice were studied. Main clinical signs of cough, asthma, sneezing, cachexia, dyspnea could be observed in the chickens and ostriches that infected with the oocysts ,and the signs in ostriches was mild,but there was no symptom as that of described as above in mice and no oocysts were found in their feces. The regulation of shedding oocysts in chicken was different with it in ostrich ,there were multiple peaks in ostrich.The experiments were carried out on pathology of some organs in young ostriches that infected with Cryptosporidium from ostriches. We found that Cryptosporidium derived from ostrich mainly parasitized in cloaca and bursa of Fabricious of ostriches, mainly caused mucous membrane tumefaction and epithelium cell swell,.The oocyst is identified as Cryptosporidium baileyi according to its’ morphological characteristics, the result of pathogenicity to ostriches, chickens and mice.

Key words: Ostrich , Cryptosporidium , Epidemiology, electron microscope , Characteristic