关键词: 烟蚜茧蜂, 扫描电镜, 触角感觉器, 低温冷藏, 畸形

Abstract: 【Objective】 The objectives of this study are to describe the external morphology of the antennae and antennal sensilla of Aphidius gifuensis, examine the effects of cold storage on the antennal sensilla, and to provide the basis for effective use of A. gifuensis . 【Method】 Scanning electron microscopy was used to observe the morphological characteristics of the antennae and sensilla of A. gifuensis adults that emerged under normal conditions, and to compare the sensilla of males and females. Mature larvae of A. gifuensis were stored at 4℃ for 7, 14, 21, and 28 days, and then following cold storage, samples were prepared by ethanol dehydration. Scanning electron microscopy was used to study the effects of cold storage on the external form of the antennae and antennal sensilla. 【Result】 The antennae of males and females were both filiform and had similar morphologies. The average length of the male antenna (19 segments) was slightly longer than that of the female (17 segments). Six types of antennal sensilla were identified, namely, trichodea, chaetica, placodea, Böhm’s bristles, coeleoconica, and basiconica sensilla. Sensilla trichodea type I, which were the most widely distributed and most numerous of the antennal sensilla, were found mainly on the flagella. Sensilla placodea were also distributed on the flagella, occurring from the first flagellum in males and from the second flagellum in females. Sensilla coeleoconica type II were found only on the antennae of adult males. There were differences in the number of different sensilla on male and female antennae. The number of sensilla chaetica type I in females was greater than that in males, whereas the converse was true for the number of sensilla chaetica type II and sensilla placodea. Abnormal placodea and coeleoconica were observed when larvae were stored at 4℃ for 14, 21, and 28 days, with the low temperature influencing the segmentation of the last two antennal subsegments. Fifty-four percent of male antennae showed abnormal segmentation, with antennae comprising 18 segments instead of the normal 19. Similarly, 69% of female antennae showed abnormal segmentation, bearing 16 segments instead of the typical 17. 【Conclusion】The antennae of adult male and female A. gifuensis are filiform. Six types of antennal sensilla were found on the antennae. The types of antennal sensilla in both males and females were similar, but the length of the male antenna was longer than that of the female. Cold storage had a significant effect on antennal segmentation and the form of some sensilla. In order to reduce the damage to A. gifuensis caused by cold storage,preserving A. gifuensis prior to the pupal stageshould be avoided and the storage time should be shortened.

Key words:  Aphidius gifuensis, scanning electron microscopy, antennal sensilla, cold storage, deformity