中国农业科学 ›› 2007, Vol. 40 ›› Issue (6): 1150-1158 .

• 耕作栽培·生理生态 • 上一篇    下一篇

两种化学型鱼腥草不同生育期净光合速率和蒸腾速率及挥发油化学成分的比较研究

黄春燕,吴 卫,郑有良,陈 黎,刘 雷,刘仁建   

  1. 四川农业大学农学院
  • 收稿日期:2006-02-17 修回日期:1900-01-01 出版日期:2007-06-10 发布日期:2007-06-10
  • 通讯作者: 吴 卫

Analysis on the photosynthesis and volatile components from the leaves of different Houttuyniae chemotypes at different growth stages

  

  1. 四川农业大学农学院
  • Received:2006-02-17 Revised:1900-01-01 Online:2007-06-10 Published:2007-06-10

摘要: 【目的】 研究两种化学型鱼腥草不同生育期叶片净光合速率和蒸腾速率以及挥发油化学成分的差异。【方法】用便携式光合仪测定自然条件下两种化学型鱼腥草苗期和花期叶片的净光合速率(Pn)和蒸腾速率(Tr)日变化。用水蒸气蒸馏法分别提取挥发油,并用GC-MS法对各化合物进行鉴定,峰面积归一法计算各成分相对含量。LSD多重比较法进行差异显著性检验。【结果】两种化学型鱼腥草Pn日变化苗期均为典型的单峰曲线,而花期呈双峰形;Tr日变化则两个时期均为单峰曲线;且苗期Pn和Tr值均极显著低于花期。两种化学型鱼腥草叶片挥发油所含组分数目苗期普遍比花期多,而共有成分和主要成分总量分别低于花期。M型鱼腥草叶片挥发油所含化学成分种类较D型多,而其挥发油化学成分中共有成分和主要成分总量却较D型低,且该化学型不同材料间在某些主要成分含量上存在极显著差异。【结论】不同化学型鱼腥草在不同生育期的净光合速率和蒸腾速率以及挥发油化学成分上均存在一定程度的差异。而峨眉蕺菜与蕺菜之间,无论在净光合速率和蒸腾速率还是挥发油化学成分方面均无显著差异。

关键词: 鱼腥草, 化学型, 生育期, 净光合速率, 蒸腾速率, 挥发性成分

Abstract: The diurnal variation of photosynthesis for the leaves of two different Houttuyniae chemotypes at different growth stages had been studied under the natural conditions, as well as their volatile chemical constituents were separated and identified by GC-MS. The results were as follows: The curve of diurnal variations of net photosynthetic rate (Pn) tended to be typical “single peak” type at seedling stage and “double peak” type at flowering stage. Tr diurnal variation curve of both stage s were “single peak” type. The numbers of chemical constituent were much more in Myrcene chemotype’s and at seedling stage than those in Decanal chemotype’s at flowering stage, respectively. However, the total contents of common and major chemical constituent were lower in Myrcene chemotype’s and at seedling stage than those in Decanal chemotype’s at flowering stage, respectively. Furthermore, there were extremely significant differences among some major chemical constituents in different accessions at different growth stages. In conclusion, different Houttuyniae chemotypes at different growth stages showed certain differences on photosynthesis and volatile chemical constituents. The result also indicated that there were no significant difference between Houttuynia emeiensis and Houttuynia cordata in both photosynthesis and their volatile chemical constituents.

Key words: Houttuyniae, chemotype, growth stage, net photosynthesis, volatile chemical constituents