关键词: 葡萄, 高温, 低温, 交叉适应, Ca2+-ATPase

Abstract: 【Objective】The aim of the experiment is to explore the mechanism of cross adaptation to different temperature stresses in the plants.【Method】Leaves from one-year-old young grape (Vitis vinifera L. cv. Jingxiu) plants were used as experimental materials in this experiment. The changes of activity of plasma membrane Ca2+-ATPase both in chilling-treated plants after heat acclimation (HA, 38℃/10 h) and heat-treated plants after cold acclimation (CA, 8℃/2.5 d) were analyzed with biochemistry assay and electromicroscopic-cytochemical assay in which cerium trichloride (CeCl3) precipitation method was adopted. 【Result】The plasma membrane Ca2+-ATPase activity was increased by HA or CA pretreatments. There was no obvious change in tonoplast Ca2+-ATPase activity after HA or CA. However, the stability of Ca2+-ATPase was enhanced under the subsequent cross-temperatures stresses, and the activity was always kept at higher level than that of control. 【Conclusion】This result from biochemistry assay agreed with the observation through the electro-microscopic technology, which suggested that plasma membrane Ca2+-ATPase was involved in the chilling resistance induced by heat HA and the thermotolerance of grape plants induced by CA and almost had the same regulating mechanism.

Key words: Grape (Vitis vinifera L.), Heat acclimation, Cold acclimation, Cross adaptation, Ca2+-ATPase