中国农业科学 ›› 2006, Vol. 39 ›› Issue (12): 2577-2581 .

• 畜牧·兽医·资源昆虫 • 上一篇    下一篇

羔羊肝脏IGF-I和IGF-I R基因表达的发育性变化研究

刘国庆,黄治国,刘振山,王新华,刘守仁,杨利国   

  1. 安徽农业大学动物科技学院
  • 收稿日期:2005-06-28 修回日期:2005-12-08 出版日期:2006-12-10 发布日期:2006-12-10
  • 通讯作者: 杨利国

Studies on The Developmental Patterns of IGF-Ⅰand IGF-ⅠR Gene Expression in Liver Tissue of Lamb

  1. 安徽农业大学动物科技学院
  • Received:2005-06-28 Revised:2005-12-08 Online:2006-12-10 Published:2006-12-10

摘要: 【目的】通过对羔羊IGF-Ⅰ和 IGF-ⅠR基因表达的发育性变化的研究,为羔羊生长发育规律提供理论依据。【方法】选择2、30、60、90和120日龄的雄性哈萨克羊和新疆细毛羊各6只(共54只,120日龄只有新疆细毛羊),测体重后屠宰,采取肝脏,用荧光实时定量PCR法,以GAPDH基因为内标,检测IGF-Ⅰ和IGF-ⅠR基因的发育性变化,并进行品种之间比较。【结果】(1)肝脏IGF-Ⅰ基因的表达量都呈先升后降的趋势,其中雄性哈萨克羊肝脏IGF-Ⅰ基因的表达量从2日龄到60日龄持续上升,60日龄后开始下降,90日龄的表达量显著低于前三个时期(P<0.05);雄性新疆细毛羊肝脏IGF-Ⅰ基因的表达量从2日龄到90日龄持续上升,90日龄后开始下降,120日龄的表达量显著低于60日龄(P<0.05)。雄性哈萨克羊肝脏IGF-Ⅰ基因的表达量在2日龄时与新疆细毛羊差异不显著(P>0.05),在30~60日龄期间都显著高于新疆细毛羊(P<0.05),在90日龄时极显著高于新疆细毛羊(P<0.01);(2)肝脏IGF-ⅠR基因的表达量都呈现持续下降的趋势,其中哈萨克羊肝脏IGF-ⅠR基因在2日龄的表达量最高,然后就持续下降,2日龄时的表达量与其他各时期差异显著(P<0.05);新疆细毛羊肝脏IGF-ⅠR基因在2日龄的表达量最高,然后就持续下降,2日龄时的表达量与其他各时期差异显著(P<0.05)。哈萨克羊肝脏IGF-ⅠR基因的表达量在2和90日龄时都极显著低于新疆细毛羊(P<0.01)。【结论】羔羊肝脏组织中IGF-Ⅰ和IGF-ⅠR的基因表达量有特定的发育模式,IGF-ⅠR的基因表达水平的变化不依赖IGF-Ⅰ的基因表达水平。

关键词: 羔羊, 肝脏, IGF-Ⅰ, IGF-ⅠR, 荧光实时定量PCR

Abstract: Abstract:In present experiment, A Real time polymerase chain reaction (PCR) was applied to investigate the development patterns of IGF-Ⅰ, IGF-ⅠR mRNA expression in liver tissue of lamb, and GAPDH internal standards were used as control. Fifty-fourth of XJXM and HSK sheep were sampled at 2,30,60,90,120 days of age respectively (Only there have sixth of 120 days of age in XJXM sheep). The results showed that (1) IGF-ⅠmRNA expression levels in liver tissue of XJXM and HSK sheep follow the same developmental pattern. The expression levels of IGF-ⅠmRNA in liver tissue increased firstly and declined thereafter gradually. The expression of IGF-ⅠmRNA in HSK sheep was increased gradually between 2 days of age and 60 days of age, and declined gradually after 60 days of age. The expression of IGF-ⅠmRNA in XJXM sheep was increased gradually between 2 days of age and 90 days of age, and declined gradually after 90 days of age. The expression of IGF-ⅠmRNA in HSK sheep was higher than that in XJXM sheep at 30 and 60 days of age (P<0.05), and at 90 days of age (P<0.01). (2) IGF-ⅠR mRNA expression levels in liver tissue of XJXM and HSK sheep follow the same developmental pattern. The expression levels of IGF-ⅠmRNA in liver tissue declined gradually. The expression of IGF-ⅠmRNA in HSK sheep was highest at 2 days of age and declined gradually thereafter. The expression of IGF-ⅠR mRNA in HSK sheep at 2 days of age was higher than that at the other days of age(P<0.05). The expression of IGF-ⅠmRNA in XJXM sheep was highest at 2 days of age and declined gradually thereafter. The expression of IGF-ⅠR mRNA in XJXM sheep at 2 days of age was higher than that at the other days of age(P<0.05). The expression of IGF-ⅠR mRNA in HSK sheep was lower than that in XJXM sheep at 2 and 90 days of age (P<0.01). The result show that the IGF-Ⅰand IGF-ⅠR mRNA expression in liver tissue follow specific developmental pattern respectively. Level of IGF-ⅠR mRNA expression does not depend on that of IGF-ⅠmRNA expression.

Key words: Lamb, (Insulin like growth factorⅠ) IGF-Ⅰ, (Insulin like growth factorⅠreceptor) IGF-ⅠR, Liver tissue, Real time polymerase chain reaction