中国农业科学 ›› 2020, Vol. 53 ›› Issue (2): 398-408.doi: 10.3864/j.issn.0578-1752.2020.02.014
郑琛1,李发弟2,3,李飞2,周巨旺1,段鹏伟1,刘绘汇1,樊海苗1,朱威力1,刘婷1()
收稿日期:
2019-06-24
接受日期:
2019-08-14
出版日期:
2020-01-16
发布日期:
2020-02-17
通讯作者:
刘婷
作者简介:
郑琛,E-mail:zhengc@gsau.edu.cn。
基金资助:
ZHENG Chen1,LI FaDi2,3,LI Fei2,ZHOU JuWang1,DUAN PengWei1,LIU HuiHui1,FAN HaiMiao1,ZHU WeiLi1,LIU Ting1()
Received:
2019-06-24
Accepted:
2019-08-14
Online:
2020-01-16
Published:
2020-02-17
Contact:
Ting LIU
摘要:
【目的】探讨代乳粉中添加甘露寡糖(mannan oligosaccharides,MOS)对7—28日龄湖羊羔羊胃肠道生长发育的影响。【方法】选择同质性良好的7日龄湖羊公羔(双羔)30只,随机分为2组,每组15只,每只为1个重复,对照组羔羊饲喂不含MOS的代乳粉,试验组羔羊饲喂含0.2 % MOS的代乳粉,试验期21d。羔羊28日龄时,两个试验组各随机选择8只羔羊屠宰,取出消化道,称量各胃室和肠段包含内容物的质量和净质量,量取各肠段长度,用以计算各部位的相对质量和内容物分布,以及各肠段的相对长度。多聚甲醛固定皱胃胃底腺区及十二指肠、空肠和回肠中段的组织样品,测定组织形态和小肠上皮细胞凋亡率。采集十二指肠、空肠和回肠的黏膜样品,测定紧密连接蛋白1 (claudin 1)、闭锁小带1(zonula occludens-1,ZO-1)和闭锁蛋白(occludin)的mRNA表达量。【结果】除空肠相对长度外(%全肠长度,P=0.040),MOS对羔羊胃肠指数(%活体质量)、胃肠相对质量(%全胃质量、%全肠质量和%全胃肠质量)、肠道相对长度(%全肠长度)、内容物相对活体质量(%活体质量)、胃肠内容物相对总胃/肠内容物及总胃肠内容物相对质量(%总胃内容物、总肠内容物、总胃肠内容物)、小肠上皮细胞凋亡率和小肠黏膜claudin 1蛋白mRNA的表达量均没有产生显著影响(P>0.05),但MOS显著提高羔羊十二指肠绒毛高度和肌层厚度并显著降低绒毛宽度(P=0.033,P=0.047,P=0.015),显著上调空肠ZO-1蛋白mRNA表达量(P=0.028),此外,MOS有提高羔羊回肠绒毛高度、绒毛宽度和隐窝深度、皱胃肌层厚度及回肠occludin蛋白mRNA表达量的趋势(P=0.075,P=0.078,P=0.085,P=0.084,P=0.052)。【结论】MOS对7—28日龄湖羊羔羊胃肠道相对质量、长度和内容物分布基本无显著影响,但可改善十二指肠和回肠绒毛及肌层的组织形态,维持小肠屏障功能,有利于提高养分消化率。
郑琛,李发弟,李飞,周巨旺,段鹏伟,刘绘汇,樊海苗,朱威力,刘婷. 代乳粉添加甘露寡糖对7—28日龄湖羊羔羊胃肠道发育的影响[J]. 中国农业科学, 2020, 53(2): 398-408.
ZHENG Chen,LI FaDi,LI Fei,ZHOU JuWang,DUAN PengWei,LIU HuiHui,FAN HaiMiao,ZHU WeiLi,LIU Ting. Effects of Adding Mannan Oligosaccharides to Milk Replacer on the Development of Gastrointestinal Tract of 7-28 Days Old Hu Lambs[J]. Scientia Agricultura Sinica, 2020, 53(2): 398-408.
表2
MOS对羔羊胃肠指数的影响"
项目Item | 对照组Control | MOS组 MOS | SEM | P值 P value |
---|---|---|---|---|
胃室 Stomach | ||||
瘤胃 Rumen | 0.54 | 0.50 | 0.02 | 0.386 |
网胃 Reticulum | 0.16 | 0.15 | 0.01 | 0.555 |
瓣胃 Omasum | 0.08 | 0.07 | 0.01 | 0.488 |
皱胃 Abomasum | 1.04 | 0.92 | 0.04 | 0.138 |
肠道 Intestinal tract | ||||
十二指肠 Duodenum | 0.20 | 0.17 | 0.01 | 0.066 |
空肠Jejunum | 4.00 | 3.71 | 0.14 | 0.316 |
回肠 Ileum | 0.13 | 0.14 | 0.01 | 0.697 |
盲肠 Cecum | 0.35 | 0.41 | 0.02 | 0.201 |
结肠 Colon | 1.18 | 1.15 | 0.04 | 0.675 |
直肠 Rectum | 0.38 | 0.39 | 0.01 | 0.663 |
表3
MOS对羔羊胃肠相对质量的影响"
项目Item | 对照组Control | MOS组 MOS | SEM | P值 P value | |
---|---|---|---|---|---|
胃室 Stomach | |||||
瘤胃 Rumen | %全胃质量 % the quality of total stomach | 29.70 | 30.45 | 0.58 | 0.533 |
%全胃肠质量 % the quality of total gastrointestinal tract | 6.71 | 6.62 | 0.27 | 0.869 | |
网胃 Reticulum | %全胃质量 % the quality of total stomach | 9.16 | 9.46 | 0.40 | 0.724 |
%全胃肠质量 % the quality of total gastrointestinal tract | 2.05 | 2.06 | 0.10 | 0.939 | |
瓣胃 Omasum | %全胃质量 % the quality of total stomach | 4.08 | 4.15 | 0.21 | 0.883 |
%全胃肠质量 % the quality of total gastrointestinal tract | 0.92 | 0.89 | 0.05 | 0.795 | |
皱胃 Abomasum | %全胃质量 % the quality of total stomach | 57.05 | 55.94 | 0.74 | 0.471 |
%全胃肠质量 % the quality of total gastrointestinal tract | 12.81 | 12.14 | 0.35 | 0.356 | |
肠道 Intestinal tract | |||||
十二指肠 Duodenum | %全肠质量 % the quality of total intestinal tract | 3.22 | 2.93 | 0.14 | 0.297 |
%全胃肠质量 % the quality of total gastrointestinal tract | 2.49 | 2.28 | 0.09 | 0.292 | |
空肠Jejunum | %全肠质量 % the quality of total intestinal tract | 64.21 | 61.69 | 1.07 | 0.254 |
%全胃肠质量 % the quality of total gastrointestinal tract | 49.80 | 48.36 | 1.06 | 0.517 | |
回肠 Ileum | %全肠质量 % the quality of total intestinal tract | 1.91 | 2.41 | 0.21 | 0.258 |
%全胃肠质量 % the quality of total gastrointestinal tract | 1.69 | 1.88 | 0.13 | 0.477 | |
盲肠 Cecum | %全肠质量 % the quality of total intestinal tract | 5.57 | 6.89 | 0.44 | 0.135 |
%全胃肠质量 % the quality of total gastrointestinal tract | 4.32 | 5.38 | 0.34 | 0.116 | |
结肠 Colon | %全肠质量 % the quality of total intestinal tract | 18.95 | 19.43 | 0.57 | 0.686 |
%全胃肠质量 % the quality of total gastrointestinal tract | 14.67 | 15.17 | 0.41 | 0.558 | |
直肠 Rectum | %全肠质量 % the quality of total intestinal tract | 6.14 | 6.65 | 0.23 | 0.275 |
%全胃肠质量 % the quality of total gastrointestinal tract | 4.75 | 5.19 | 0.16 | 0.162 |
表5
MOS对羔羊胃肠内容物相对活体质量的影响"
项目Item | 对照组Control | MOS组 MOS | SEM | P值 P value |
---|---|---|---|---|
胃室内容物 Stomach content | ||||
瘤胃内容物 Rumen content | 1.44 | 1.67 | 0.20 | 0.587 |
网胃内容物 Reticulum content | 0.24 | 0.34 | 0.07 | 0.473 |
瓣胃内容物 Omasum content | 0.01 | 0.01 | 0.001 | 0.946 |
皱胃内容物 Abomasum content | 3.43 | 3.18 | 0.24 | 0.623 |
肠道内容物 Intestinal tract content | ||||
十二指肠内容物 Duodenum content | 0.15 | 0.14 | 0.02 | 0.868 |
空肠内容物Jejunum content | 1.26 | 1.35 | 0.20 | 0.833 |
回肠内容物 Ileum content | 0.11 | 0.05 | 0.03 | 0.378 |
盲肠内容物 Cecum content | 0.43 | 0.30 | 0.08 | 0.422 |
结肠内容物 Colon content | 1.08 | 0.81 | 0.13 | 0.298 |
直肠内容物 Rectum content | 0.17 | 0.21 | 0.03 | 0.503 |
表6
MOS对羔羊胃肠内容物相对总胃/肠内容物及总胃肠内容物相对质量的影响"
项目 Item | 对照组Control | MOS组 MOS | SEM | P值 P value | |
---|---|---|---|---|---|
胃室内容物 Stomach content | |||||
瘤胃内容物 Rumen content | %全胃内容物质量 % the quality of total stomach content | 26.96 | 33.15 | 2.56 | 0.239 |
%全胃肠内容物质量 % the quality of total gastrointestinal tract content | 17.30 | 21.12 | 1.80 | 0.306 | |
网胃内容物 Reticulum content | %全胃内容物质量 % the quality of total stomach content | 4.99 | 6.68 | 1.28 | 0.532 |
%全胃肠内容物质量 % the quality of total gastrointestinal tract content | 3.50 | 4.06 | 0.03 | 0.742 | |
瓣胃内容物 Omasum content | %全胃内容物质量 % the quality of total stomach content | 0.23 | 0.24 | 0.03 | 0.988 |
%全胃肠内容物质量 % the quality of total gastrointestinal tract content | 0.14 | 0.13 | 0.01 | 0.698 | |
皱胃内容物 Abomasum content | %全胃内容物质量 % the quality of total stomach content | 68.58 | 60.09 | 2.71 | 0.119 |
%全胃肠内容物质量 % the quality of total gastrointestinal tract content | 43.14 | 39.30 | 2.35 | 0.434 | |
肠道内容物 Intestinal tract content | |||||
十二指肠内容物 Duodenum content | %全肠内容物质量 % the quality of total intestinal tract content | 7.20 | 6.23 | 1.63 | 0.777 |
%全胃肠内容物质量 % the quality of total gastrointestinal tract content | 1.98 | 2.22 | 0.39 | 0.765 | |
空肠内容物 Jejunum content | %全肠内容物质量 % the quality of total intestinal tract content | 39.34 | 46.21 | 2.22 | 0.126 |
%全胃肠内容物质量 % the quality of total gastrointestinal tract content | 14.28 | 16.95 | 1.69 | 0.451 | |
回肠内容物 Ileum content | %全肠内容物质量 % the quality of total intestinal tract content | 1.75 | 1.45 | 0.31 | 0.666 |
%全胃肠内容物质量 % the quality of total gastrointestinal tract content | 0.80 | 0.57 | 0.20 | 0.618 | |
盲肠内容物 Cecum content | %全肠内容物质量 % the quality of total intestinal tract content | 12.73 | 9.88 | 1.35 | 0.312 |
%全胃肠内容物质量 % the quality of total gastrointestinal tract content | 4.88 | 3.51 | 0.70 | 0.345 | |
结肠内容物 Colon content | %全肠内容物质量 % the quality of total intestinal tract content | 33.02 | 30.57 | 2.83 | 0.681 |
%全胃肠内容物质量 % the quality of total gastrointestinal tract content | 12.49 | 10.25 | 1.17 | 0.356 | |
直肠内容物 Rectum content | %全肠内容物质量 % the quality of total intestinal tract content | 5.99 | 7.80 | 1.05 | 0.405 |
%全胃肠内容物质量 % the quality of total gastrointestinal tract content | 2.06 | 2.77 | 0.42 | 0.414 |
表7
MOS对羔羊皱胃和小肠组织形态的影响"
项目Item | 对照组Control | MOS组 MOS | SEM | P值 P value |
---|---|---|---|---|
皱胃 Abomasum | ||||
黏膜厚度 Mucosa thickness | 333.36 | 331.46 | 9.74 | 0.927 |
肌层厚度 Muscular thickness | 548.48 | 686.99 | 40.11 | 0.084 |
十二指肠 Duodenum | ||||
绒毛高度 Villus height | 361.99b | 446.39a | 20.44 | 0.033 |
绒毛宽度 Villus width | 80.04a | 61.96b | 3.92 | 0.015 |
隐窝深度 Crypt depth | 148.87 | 163.33 | 8.46 | 0.414 |
肌层厚度 Muscular thickness | 161.93b | 201.87a | 10.25 | 0.047 |
绒毛高度/隐窝深度值V/C | 2.57 | 2.82 | 0.11 | 0.258 |
空肠Jejunum | ||||
绒毛高度 Villus height | 431.59 | 445.30 | 20.29 | 0.748 |
绒毛宽度 Villus width | 71.13 | 68.01 | 2.04 | 0.464 |
隐窝深度 Crypt depth | 173.48 | 158.21 | 6.65 | 0.265 |
肌层厚度 Muscular thickness | 157.73 | 153.01 | 8.27 | 0.786 |
绒毛高度/隐窝深度值V/C | 2.59 | 2.89 | 0.09 | 0.118 |
回肠 Ileum | ||||
绒毛高度 Villus height | 373.81 | 428.38 | 15.25 | 0.075 |
绒毛宽度 Villus width | 68.91 | 78.87 | 2.81 | 0.078 |
隐窝深度 Crypt depth | 176.96 | 213.10 | 10.38 | 0.085 |
肌层厚度 Muscular thickness | 274.31 | 230.82 | 18.54 | 0.267 |
绒毛高度/隐窝深度值V/C | 2.19 | 2.15 | 0.06 | 0.775 |
表8
MOS对羔羊小肠黏膜紧密连接蛋白mRNA表达量及上皮细胞凋亡的影响"
项目Item | 对照组Control | MOS组 MOS | SEM | P值 P value |
---|---|---|---|---|
十二指肠 Duodenum | ||||
Claudin 1 | 0.98 | 0.94 | 0.10 | 0.863 |
ZO-1 | 0.92 | 1.05 | 0.08 | 0.403 |
Occludin | 0.86 | 1.18 | 0.10 | 0.112 |
细胞凋亡 Apoptosis (%) | 11.27 | 5.04 | 2.90 | 0.302 |
空肠Jejunum | ||||
Claudin 1 | 0.91 | 1.09 | 0.21 | 0.671 |
ZO-1 | 0.80b | 1.20a | 0.10 | 0.028 |
Occludin | 0.96 | 1.04 | 0.08 | 0.630 |
细胞凋亡 Apoptosis (%) | 21.09 | 18.08 | 5.40 | 0.791 |
回肠 Ileum | ||||
Claudin 1 | 0.87 | 0.81 | 0.18 | 0.875 |
ZO-1 | 0.92 | 1.06 | 0.07 | 0.362 |
Occludin | 0.83 | 1.17 | 0.09 | 0.052 |
细胞凋亡 Apoptosis (%) | 47.29 | 37.62 | 6.54 | 0.487 |
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