关键词: 小麦体细胞杂种, 耐盐基因, SSR标记, 染色体定位

Abstract: 【Objective】 Screen and localize the SSR markers related to salt tolerance trait in wheat variety Shanrong No.3. 【Method】Using a novel salt-tolerant wheat asymmetric somatic hybrid variety Shanrong No.3 as experimental material, the F2 population of hybridized combination 01 was constructed from sexual crossing between Shanrong No.3 and salt-sensitive variety Jinan 17. In combination to the SSR map of wheat, the seedlings of the population were used to determine the salt tolerance and the loci related to the salt tolerance was tagged by microsatellite (SSR) and BSA (bulked segregant analysis). 【Result】Genetic analysis and chi-square test of salt-tolerant phenotype of F2 population showed that the salt tolerance of the combination 01 was likely controled by major gene. A wheat specific SSR marker Xgwm304 was decided to link with salt-tolerant locus of wheat variety Shanrong No.3. 【Conclusion】The genetic distance between Xgwm304 and the critical gene is 24.41 cM (centMorgan). The major salt tolerance gene in wheat variety Shanrong No.3 has been located on 5AS of wheat chromosome.

Key words: wheat somatic hybrids, salt tolerance gene, SSR (microsatellite DNA) marker, chromosome mapping