关键词: 作物遗传学, 玉米, 幼胚培养, 遗传模型, 基因效应

Abstract: 【Objective】The research aim to pick out the hereditary variation regularity for the main characters of the maize immature embryo culture. 【Method】Two kinds of inbred-line, R18-599 (hong) with very excellent embryo culturing capacity and R15 with very bad embryo culturing capacity, were used as P1 and P2 for obtaining 6 generations. By culturing immature embryos of the six generations, four culturing characters, embryonic callus induction efficiency, non-embryonic callus induction efficiency, embryonic callus clone ability and number of regenerating plant, were analyzed with the general mean analysis and generation joint analysis.【Result】Results showed that the embryonic callus induction efficiency accorded with two major additive-domiance-epistatic genes plus polygene mixed additive-domiance-epistatic inheritance model. The non-embryonic callus induction efficiency accorded with two major additive-domiance-epistatic genes. The number of regenerating plant accorded with one major gene plus polygene mixed additive-dominance inheritance model. The embryo callus clone ability accorded with two major genes plus polygene mixed inheritance model while the epistatic gene effect on this character identified differently by the two methods. 【Conclusion】By comparison of the results of the two methods, generation joint analysis may not only raise experimental precision, but also provide more genetic information.

Key words: Crop genetics, Maize (Zea mays L.), Immature embryo culture, Inheritance model, Gene effect