关键词: 小麦, 胚, 穗发芽, ABA敏感性, 蛋白质组

Abstract: There is a significant relationship between resistance to sprouting and ABA sensitivity in wheat. Variance of protein expression will take place after ABA treatment. In this study, wheat embryos isolated from resistant cultivar Shanyou 225 and susceptible cultivar Jimai 1 at 25th day after anthesis were incubated with or without ABA solution to identify ABA sensitivity, and then protein expression in the embryos was studied by proteome analysis. The results indicated that seven protein spots could be recognized according to the level of expression. They are NAD(P)H dehydrogenase, late embryogenesis abundant (LEA) protein Dc3, actin-depolymerizing factor 5 (ADF-5), calmodulin-related protein, salt-stress root protein RS1, 20 kD calcium-binding protein and auxin-responsive protein IAA19. These proteins could be divided into two categories. The abundance of some proteins, such as LEA, in dormant embryo was higher than that of non-dormant embryo，the expression level of these proteins are up-regulated by ABA. On the other hand, the auxin-responsive protein IAA19 with higher abundance in non-dormant embryo than dormant embryo was identified, its expression level was down-regulated by ABA. The proteins up-regulated by ABA are prone to express in water than ABA treatment in embryos of sprouting resistant genotype. These data are useful to study protein expression process of sprouting resistant genes.

Key words: Common wheat, Embryo, Pre-harvest Sprouting, ABA sensitivity, Proteome