关键词: 家蚕, 全茧量, AFLP, QTL, 多重区间分析

Abstract: A backcrossed population (BC1) derived from a cross between C100 and Dazao was obtained. It was the first time for the QTLs of the economically important traits of whole cocoon weight, cocoon shell weight, ratio of cocoon shell and weight of pupae and so on to be analyzed by the Multiple Interval Mapping software WinQTLCart2.0. And totally 40 QTLs were detected and contributed in 21 groups based on the constructed linkage map. According to the mapping results, 2, 2, 3, and 2 major QTLs explained over 20% of the total phenotypic variations, while there were four QTLs as qCW-19, qSW-2, qCSR-4 and qPW-23 explained more than 30% of which. All of the above QTLs were detected for whole cocoon weight, cocoon shell weight, ratio of cocoon shell and weight of pupae, respectively. Correlated traits QTLs often share the same location. Furthermore, most of the detected QTL were closed to one-side marker. By using the very closed markers, the authors have aggregated positive QTLs and built a basis for molecular marker assisted selection and breeding.

Key words: Bombyx mori, Whole cocoon weight, AFLP, QTL, Multiple interval mapping