中国农业科学 ›› 2005, Vol. 38 ›› Issue (07): 1295-1299 .

• 作物遗传育种.种质资源 • 上一篇    下一篇

小麦多酚氧化酶(PPO)活性的SSR标记筛选鉴定与验证

孙道杰,何中虎,张立平,夏先春,葛秀秀,徐兆华,王 辉   

  1. 中国农业科学院作物所
  • 收稿日期:2004-06-23 修回日期:2004-10-20 出版日期:2005-07-10 发布日期:2005-07-10
  • 通讯作者: 何中虎

Validation of SSR Marker Assisted Selection for Polyphenol Oxidase Activities in Common Wheat

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  1. 中国农业科学院作物所
  • Received:2004-06-23 Revised:2004-10-20 Online:2005-07-10 Published:2005-07-10

摘要: 小麦多酚氧化酶(PPO)活性是引起面条和面团储存期间颜色褐变的主要因素。发掘可应用于籽粒PPO活性辅助选择的分子标记,将有助于小麦面粉颜色性状的遗传改良。本研究选用来自全国不同麦区的203份冬小麦品种,验证SSR(simple sequence repeat)引物Xgwm312的PCR扩增片段大小与籽粒PPO活性的关系。结果表明,198bp扩增片段的有无同籽粒PPO活性大小密切相关,该片段的出现通常意味着籽粒PPO活性较高。Xgwm312可应用于籽粒PPO活性的分子标记辅助育种。

关键词: 普通小麦, 多酚氧化酶(PPO), SSR标记, 分子标记辅助选择

Abstract: Polyphenol oxidase (PPO) is highly related to undesirable brown discoloration of wheat-based end products such as noodles. It is important to identify molecular markers associated with PPO activities for marker-assisted selection (MAS) for wheat quality improvement. A total of 203 Chinese winter wheat cultivars and lines were analyzed to investigate the correlation of PPO activities with a SSR(simple sequence repeat)marker Xgwm312. The results indicated that a fragment with 198 bp is apparently correlated with high PPO activities in wheat grain. The marker Xgwm312 can be used for MAS for PPO activities.

Key words: Triticum aestivum L., Polyphenol oxidase, SSR marker, MAS