中国农业科学 ›› 2006, Vol. 39 ›› Issue (11): 2194-2199 .

• 农业信息技术 • 上一篇    下一篇

烟草种质资源遗传多样性及亲缘关系的AFLP分析

杨友才,周清明,尹晗琪,朱列书   

  1. 湖南农业大学烟草工程技术研究中心
  • 收稿日期:2005-06-07 修回日期:2006-08-11 出版日期:2006-11-10 发布日期:2006-11-10
  • 通讯作者: 杨友才

Studies on the Genetic Diversity and Relationship of Tobacco Germplasms by AFLP Analysis

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  1. 湖南农业大学烟草工程技术研究中心
  • Received:2005-06-07 Revised:2006-08-11 Online:2006-11-10 Published:2006-11-10

摘要: 【目的】揭示烟草种质资源的遗传多样性及亲缘关系。【方法】选用4对AFLP选择性扩增引物(EcoRI+3/MseI+3)对48份烟草材料的基因组DNA进行选择性扩增。【结果】共获得321条扩增带,其中174条具有多态性,平均多态检出率为54.2%。对AFLP扩增结果采用UPGMA法进行聚类分析,可以将48份烟草资源分为两大类群,即黄花烟草群和普通烟草群,普通烟草可进一步分为4组;48份材料的遗传距离变幅在1.41~11.0之间。【结论】AFLP标记技术能较好地从分子水平揭示中国烟草种质资源的遗传背景、亲缘关系及演化规律。

关键词: 烟草, 遗传多样性, 亲缘关系, AFLP

Abstract: Abstract: AFLP molecular marker was used to detect the genetic diversity and relationship among 48 tobacco germplasms. they are amllified by 4 AFLP selective primers(EcoR Ⅰ+3/MseⅠ+3), altogether 321 AFLP bands are obtained, among them 174 are polymorphic bands, the percentage of polymorphic bands is 54.2%.UPGMA cluster analysis based on AFLP molecular markers data divide 48 varieties into two groups: N.tobacum L group and N.rustica L.group. N.tobacum L. group can be classified into four sub groups,the genetic distance of 48 cultivars is between 1.41~11.0, this cluster result basically accord with theoretic expectation case. The results show that the AFLP approach is highly efficient in generating accurate information on genetic backgroud, relationship and evolution of tobacco germplasms.

Key words: tobacco, genetic diversity, relationship, AFLP