小麦面粉色泽相关位点的育种利用效应分析

doi:10.3864/j.issn.0578-1752.2026.10.001

中国农业科学 ›› 2026, Vol. 59 ›› Issue (10): 2061-2074.doi: 10.3864/j.issn.0578-1752.2026.10.001

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

小麦面粉色泽相关位点的育种利用效应分析

翟胜男¹(), 吕莹莹¹, 何中虎², 夏先春², 马瑞峰¹, 王颖¹, 李法计¹, 曹新有¹, 李豪圣¹, 韩冉¹, 汪晓璐¹, 李吉虎¹(), 刘建军¹()

¹ 山东省农业科学院作物研究所，济南 250100
² 中国农业科学院作物科学研究所，北京 100081

收稿日期:2025-10-30 接受日期:2025-12-26 出版日期:2026-05-16 发布日期:2026-05-20
通信作者:
李吉虎，E-mail：lijihuyantai@163.com。
刘建军，E-mail：ljjsaas@163.com。
联系方式: 翟胜男，E-mail：zsn19870322@163.com。
基金资助:
山东省自然科学基金(ZR2023MC205); 山东省自然科学基金(ZR2023MC155); 山东省泰山学者工程(tspd20221108); 山东省重点研发计划(2023CXPT079-2); 山东省农业科学院创新工程(CXGC2022E01); 国家小麦产业技术体系(CARS-03-06); 济南市“新高校20条”资助项目(202228067)

Analysis of the Breeding Utilization Effects of Loci Related to Wheat Flour Color

ZHAI ShengNan¹(), LÜ YingYing¹, HE ZhongHu², XIA XianChun², MA RuiFeng¹, WANG Ying¹, LI FaJi¹, CAO XinYou¹, LI HaoSheng¹, HAN Ran¹, WANG XiaoLu¹, LI JiHu¹(), LIU JianJun¹()

¹ Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100
² Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081

Received:2025-10-30 Accepted:2025-12-26 Published:2026-05-16 Online:2026-05-20

摘要/Abstract

摘要：

【目的】面粉色泽是小麦品质评价的重要指标，鉴定面粉色泽相关位点的优异等位变异，明确其育种利用效应，为小麦面粉色泽分子标记辅助育种提供依据。【方法】利用功能标记对166份小麦品种（系）的12个面粉色泽相关位点进行分子检测，包括Psy-A1、Pds-B1、Lcye-A1、Lcye-B1、Lox-B1、Ppo-A1、Ppo-B1、Ppo-D1、Pod-A1、Pod-D1、Pod-2D和1B/1R易位，结合面粉色泽（黄色素含量（yellow pigment content，YPC）、L*值、a*值、b*值和白度）鉴定结果，分析不同等位变异对面粉色泽的影响，以期全面系统评价各位点的育种利用效应。【结果】供试材料面粉色泽变异范围较广，YPC平均值为1.18 μg·g^-1，变幅为0.57—2.96 μg·g^-1；L*值平均值为90.29，变幅为87.12—92.16；a*值平均值为-0.86，变幅为-1.78—-0.09；b*值平均值为8.83，变幅为5.21—14.69；白度平均值为86.78，变幅为81.35—90.30。环境、基因型和基因型与环境间的互作均对面粉色泽产生重要影响，且基因型对表型影响最大。Psy-A1和1B/1R易位显著影响YPC、L*值、a*值、b*值和白度；Lcye-B1显著影响YPC、a*值、b*值和白度；Pds-B1和Lox-B1显著影响L*值、b*值和白度；Pod-2D显著影响L*值和白度；Lcye-A1显著影响L*值；Ppo-A1和Ppo-D1显著影响a*值；上述9个位点显著影响面粉色泽，育种利用潜力大。含有Psy-A1b、Pds-B1b、Lcye-A1b、Lcye-B1b、Lox-B1a、Ppo-A1b、Ppo-D1a、Pod-2D-GG、Pod-2D-AG和非1B/1R易位的小麦品种（系），具有高亮白面粉色泽，将上述基因型命名为优异等位基因，分布频率分别为34.94%、20.48%、97.59%、66.27%、26.38%、50.91%、57.23%、48.80%、15.06%和51.20%。随着优异等位基因数目的增加，L*值、a*值和白度逐渐升高，YPC和b*值逐渐降低；聚合7—8个优异基因的材料面粉色泽最优。不同地区小麦品种（系）面粉色泽及相关基因等位变异频率存在较大差异。郑引1号、淄麦12和皖麦19等22个品种聚合了7个以上优异等位基因，可作为亲本材料，用于面粉高亮白小麦品种选育。【结论】Psy-A1、Pds-B1、Lcye-A1、Lcye-B1、Lox-B1、Ppo-A1、Ppo-D1、Pod-2D和1B/1R易位显著影响面粉色泽，育种实用性强；郑引1号、淄麦12和皖麦19等22个品种可作为面粉色泽改良的优异亲本。

关键词: 小麦, 面粉色泽, 分子标记, 优异等位基因, 育种利用

Abstract:

【Objective】The flour color is a crucial index for evaluating wheat quality. Identifying superior allelic variations at loci associated with flour color and clarifying their breeding utilization effects will provide a foundation for molecular marker-assisted breeding of wheat flour color.【Method】Using functional markers, molecular detection was conducted on 12 flour color-related loci in 166 wheat varieties (lines), including the Psy-A1, Pds-B1, Lcye-A1, Lcye-B1, Lox-B1, Ppo-A1, Ppo-B1, Ppo-D1, Pod-A1, Pod-D1, Pod-2D genes, and the 1B/1R translocation. Combined with the phenotypic measurements of flour color parameters (Yellow pigment content (YPC), L* value, a* value, b* value and whiteness), the influence of different allelic variations on flour color were analyzed to comprehensively and systematically evaluate the breeding utilization effects of each locus.【Result】The flour color of the tested materials exhibited a wide variation range. The average value of YPC was 1.18 μg·g^-1, with a range of 0.57-2.96 μg·g^-1; The average value of L* was 90.29, ranging from 87.12 to 92.16; the average a* value was -0.86, varying between -1.78 and -0.09; the average b* value was 8.83, with a range of 5.21-14.69; and the average whiteness was 86.78, spanning from 81.35 to 90.30. Environment, genotype, and the interaction between genotype and environment all significantly influenced flour color, with genotype exerting the strongest effect on the phenotypic variations. Psy-A1 and 1B/1R translocation significantly affected YPC, L* value, a* value, b* value and whiteness; Lcye-B1 significantly influenced YPC, a* value, b* value and whiteness; Pds-B1 and Lox-B1 significantly impacted L* value, b* value and whiteness; Pod-2D significantly influenced L* value and whiteness; Lcye-A1 significantly affected L* value; Ppo-A1 and Ppo-D1 significantly influenced a* value. These nine loci had substantial impacts on flour color and exhibited great potential for breeding applications. Wheat varieties (lines) containing Psy-A1b, Pds-B1b, Lcye-A1b, Lcye-B1b, Lox-B1a, Ppo-A1b, Ppo-D1a, Pod-2D-GG, Pod-2D-AG and non-1B/1R translocation exhibited high brightness and whiteness flour color. These genotypes were designated as superior alleles, with distribution frequencies of 34.94%, 20.48%, 97.59%, 66.27%, 26.38%, 50.91%, 57.23%, 48.80%, 15.06% and 51.20%, respectively. As the number of superior alleles increased, the L* value, a* value and whiteness gradually increased, while YPC and b* value gradually decreased. The materials pyramiding 7-8 superior genes exhibited the optimal flour color. There were significant differences in flour color and the allelic variation frequencies of related genes among wheat varieties (lines) from different regions. A total of 22 varieties, such as Zhengyin1, Zimai12 and Wanmai19, carried more than seven superior alleles and could be used as parental materials for breeding wheat varieties with high brightness and whiteness flour.【Conclusion】Psy-A1, Pds-B1, Lcye-A1, Lcye-B1, Lox-B1, Ppo-A1, Ppo-D1, Pod-2D and 1B/1R translocation significantly influence flour color and exhibit strong breeding practicability. Twenty-two wheat varieties, such as Zhengyin1, Zimai12 and Wanmai19, can be used as excellent parents for flour color improvement.

Key words: Triticum aestivum, flour color, molecular marker, superior alleles, breeding utilization

翟胜男, 吕莹莹, 何中虎, 夏先春, 马瑞峰, 王颖, 李法计, 曹新有, 李豪圣, 韩冉, 汪晓璐, 李吉虎, 刘建军. 小麦面粉色泽相关位点的育种利用效应分析[J]. 中国农业科学, 2026, 59(10): 2061-2074.

ZHAI ShengNan, LÜ YingYing, HE ZhongHu, XIA XianChun, MA RuiFeng, WANG Ying, LI FaJi, CAO XinYou, LI HaoSheng, HAN Ran, WANG XiaoLu, LI JiHu, LIU JianJun. Analysis of the Breeding Utilization Effects of Loci Related to Wheat Flour Color[J]. Scientia Agricultura Sinica, 2026, 59(10): 2061-2074.

图/表 10

表1

面粉色泽相关位点分子标记引物信息"

基因 Gene	标记 Marker	引物序列 Sequence (5′-3′)	扩增片段 Fragment size (bp)	等位基因 Allele	表型 Phenotype	参考文献 Reference
Psy-A1	YP7A	F: GGACCTTGCTGATGACCGAG	194	Psy-A1a	高YPC High YPC	[6]
Psy-A1	YP7A	R: TGACGGTCTGAAGTGAGAATGA	231	Psy-A1b	低YPC Low YPC	[6]
Pds-B1	YP4B-1	F: AAGTTGGTGGGAGTTCCTGTC	652	Pds-B1b	－	[7]
	YP4B-1	R: TGTCAGCAATGACAAAAGGATGCAT				[7]
	YP4B-2	F: AAGTTGGTGGGAGTTCCTGTC	382	Pds-B1a	－	[7]
	YP4B-2	R: GTGAACTCCTGAAACAGAAAGATTG				[7]
Lcye-A1	e-LCY3A-3	F: GCAGCAAATAACTAGACCGGGCA	537	Lcye-A1a	－	[8]
Lcye-A1	e-LCY3A-3	R: TGAACTGGGGCACAAACCACG	309 & 230	Lcye-A1b	－	[8]
Lcye-B1	YP3B-1	F: GGATCGATCTCCTGAACAGGATGTC	635	Lcye-B1a	－	[7]
Lcye-B1	YP3B-1	R: CGGCGATGCTCCGATCAGTT	No	Lcye-B1b	－	[7]
Lox-B1	LOX16	F: CCATGACCTGATCCTTCCCTT	489	Lox-B1a	高LOX活性 High LOX activity	[10]
	LOX16	R: GCGCGGATAGGGGTGGT	489	Lox-B1a	高LOX活性 High LOX activity	[10]
	LOX18	F: ACGATGTGAGTTGTGACTTGTGA	791	Lox-B1b	低LOX活性 Low LOX activity	[10]
	LOX18	R: GCGCGGATAGGGGTGC	791	Lox-B1b	低LOX活性 Low LOX activity	[10]
Ppo-A1	PPO18	F: AACTGCTGGCTCTTCTTCCCA	685	Ppo-A1a	高PPO活性 High PPO activity	[13]
Ppo-A1	PPO18	R: AAGAAGTTGCCCATGTCCGC	876	Ppo-A1b	低PPO活性 Low PPO activity	[13]
Ppo-B1	F-8	F: ACCTTGACTCCAGTGGGACC	400	Ppo-B1a	高PPO活性 High PPO activity	[14]
Ppo-B1	F-8	R: CCGAGTAGAAGTTGCCCAT	400 & 600	Ppo-B1b	低PPO活性 Low PPO activity	[14]
Ppo-D1	PPO16	F: TGCTGACCGACCTTGACTCC	713	Ppo-D1a	低PPO活性 Low PPO activity	[15]
	PPO16	R: CTCGTCACCGTCACCCGTAT	713	Ppo-D1a	低PPO活性 Low PPO activity	[15]
	PPO29	F: TGAAGCTGCCGGTCATCTAC	490	Ppo-D1b	高PPO活性 High PPO activity	[15]
	PPO29	R: AAGTTGCCCATGTCCTCGCC	490	Ppo-D1b	高PPO活性 High PPO activity	[15]
Pod-A1	POD-3A1	F: ACGGGAGACGACGAGAAGCAAAG	291	Pod-A1a	低POD活性 Low POD activity	[18]
	POD-3A1	R: TCGTGGAAGTGTAGGCGAAGA	291	Pod-A1a	低POD活性 Low POD activity	[18]
	POD-3A2	F: GTGGCGCAGGGCCTGTCA	766	Pod-A1b	高POD活性 High POD activity	[18]
	POD-3A2	R: GTTGTCGAACACGTTGGGGGA	766	Pod-A1b	高POD活性 High POD activity	[18]
Pod-D1	POD-7D1	F: GCTTCGTCCAGGACGCGTT	540	Pod-D1a	低POD活性 Low POD activity	[19]
	POD-7D1	R: CGAGGAATGGGGGGTTGATG	540	Pod-D1a	低POD活性 Low POD activity	[19]
	POD-7D6	F: TGGGCATGGGGCTTCTGCA	640	Pod-D1b	高POD活性 High POD activity	[19]
	POD-7D6	R: GCGAGGAATGGGGGGTTGATG	640	Pod-D1b	高POD活性 High POD activity	[19]
Pod-2D	Excalibur_c95720 _329-KASP	FAM: GAAGGTGACCAAGTTCATGCTccggacatcatgagggcA		Pod-2D-AA	低POD活性 Low POD activity	[20]
		HEX: GAAGGTCGGAGTCAACGGATTccggacatcatgagggcG		Pod-2D-GG	高POD活性 High POD activity
		Common: GCTCCAACTCATCCAGCGTA		Pod-2D-AG	中等POD活性 Moderate POD activity
1B/1R	H2O	F: GTTGGAAGGGAGCTCGAGCTG	1598	1B/1R	高YPC High YPC	[22]
1B/1R	H2O	R: GTTGGGCAGAAAGGTCGACATC	No	非1B/1R Non-1B/1R translocation	低YPC Low YPC	[22]

表1

表2

表3

表4

不同等位变异对面粉色泽的影响"

基因 Gene	等位基因 Allele	样本数 No. of sample	频率 Frequency (%)	黄色素含量 YPC (μg·g^-1)		*L值 L* value**		*a值 a* value**		*b值 b* value**		白度 Whiteness
基因 Gene	等位基因 Allele	样本数 No. of sample	频率 Frequency (%)	平均值±标准差 Mean±SD	范围 Range	平均值±标准差 Mean±SD	范围 Range	平均值±标准差 Mean±SD	范围 Range	平均值±标准差 Mean±SD	范围 Range	平均值±标准差 Mean±SD	范围 Range
Psy-A1	a	108	65.06	1.34±0.39**	0.62-2.96	90.09±0.95**	87.12-92.02	-0.98±0.33**	-1.78--0.09	9.57±1.89**	5.88-14.69	86.15±1.86**	81.36-89.94
Psy-A1	b	58	34.94	0.87±0.30	0.57-1.92	90.67±0.91	88.46-92.16	-0.65±0.29	-1.51--0.09	7.45±1.56	5.21-12.51	88.01±1.60	83.81-90.30
Pds-B1	a	132	79.52	1.19±0.43	0.57-2.96	90.18±0.95**	87.12-92.16	-0.86±0.34	-1.78--0.09	9.00±2.02*	5.42-14.69	86.62±1.96*	81.36-90.18
Pds-B1	b	34	20.48	1.13±0.43	0.57-2.20	90.72±0.97	88.46-92.13	-0.87±0.38	-1.67--0.13	8.12±1.84	5.21-12.51	87.48±1.94	83.16-90.30
Lcye-A1	a	4	2.41	1.04±0.31	0.68-1.42	89.13±0.11*	89.01-89.20	-0.67±0.46	-1.26--0.17	9.38±0.97	8.17-10.36	85.85±0.59	85.02-86.30
Lcye-A1	b	162	97.59	1.18±0.43	0.57-2.96	90.33±0.94	87.12-92.16	-0.87±0.35	-1.78--0.09	8.82±2.06	5.21-14.69	86.82±2.00	81.36-90.30
Lcye-B1	a	56	33.73	1.30±0.44**	0.58-2.96	90.16±0.89	88.34-91.97	-0.96±0.35**	-1.78--0.09	9.39±2.07**	5.41-13.64	86.32±1.96*	82.90-90.30
Lcye-B1	b	110	66.27	1.12±0.41	0.57-2.47	90.36±1.01	87.12-92.16	-0.82±0.34	-1.75--0.09	8.55±1.98	5.21-14.69	87.04±1.95	81.36-90.30
Lox-B1	a	43	26.38	1.14±0.40	0.61-2.04	90.57±0.95*	88.43-92.13	-0.86±0.30	-1.65--0.30	8.23±1.79*	5.81-11.85	87.25±1.98*	83.10-90.19
Lox-B1	b	120	73.62	1.20±0.44	0.57-2.96	90.20±0.96	87.12-92.16	-0.87±0.37	-1.78--0.09	9.02±2.04	5.21-14.69	86.62±1.96	81.36-90.30
Ppo-A1	a	81	49.09	1.23±0.45	0.58-2.96	90.30±0.92	88.63-92.11	-0.93±0.33*	-1.78--0.40	8.99±2.05	5.41-14.69	86.70±1.95	81.36-90.30
Ppo-A1	b	84	50.91	1.13±0.41	0.57-2.20	90.27±1.04	87.12-92.16	-0.81±0.34	-1.65--0.09	8.70±2.05	5.21-13.26	86.87±2.01	83.10-90.30
Ppo-B1	a	133	80.12	1.18±0.44	0.57-2.96	90.25±0.97	87.12-92.16	-0.86±0.36	-1.78--0.09	8.87±2.05	5.21-14.69	86.74±1.96	81.36-90.30
Ppo-B1	b	33	19.88	1.17±0.36	0.69-2.21	90.44±0.99	88.86-92.11	-0.88±0.32	-1.75--0.30	8.66±2.06	5.85-13.93	87.02±2.05	82.53-90.06
Ppo-D1	a	95	57.23	1.14±0.40	0.57-2.47	90.22±0.95	87.12-91.87	-0.81±0.32*	-1.56---0.09	8.76±2.05	5.21-14.69	86.80±1.97	81.36-90.30
Ppo-D1	b	71	42.77	1.23±0.46	0.58-2.96	90.39±1.01	88.46-92.16	-0.92±0.37	-1.78--0.09	8.93±2.05	5.41-13.64	86.80±2.01	82.90-90.30
Pod-A1	a	72	47.68	1.16±0.40	0.57-1.96	90.38±0.98	88.46-92.16	-0.86±0.35	-1.51--0.09	8.65±2.00	5.21-12.51	86.98±1.94	83.75-90.30
Pod-A1	b	79	52.32	1.18±0.41	0.60-2.47	90.18±0.96	87.12-92.02	-0.86±0.34	-1.67--0.09	8.99±1.98	5.41-14.69	86.61±1.93	81.36-90.30
Pod-D1	a	64	43.54	1.19±0.39	0.61-2.47	90.26±0.98	87.12-91.97	-0.86±0.33	-1.56--0.09	8.86±1.95	5.41-14.69	86.76±1.92	81.36-90.30
Pod-D1	b	83	56.46	1.15±0.42	0.57-2.20	90.32±0.98	88.34-92.16	-0.85±0.36	-1.67--0.09	8.73±2.03	5.21-13.26	86.88±1.96	83.10-90.30
Pod-2D	AA	60	36.14	1.16±0.48	0.57-2.96	90.05±0.83*	88.46-92.16	-0.85±0.40	-1.78--0.09	9.04±1.93	5.21-13.64	86.50±1.76*	82.90-90.30
	AG	25	15.06	1.06±0.49	0.58-2.47	90.65±1.02	88.63-92.13	-0.81±0.34	-1.75--0.38	8.09±2.44	5.42-14.69	87.55±2.34	81.36-90.19
	GG	81	48.80	1.23±0.37	0.60-2.20	90.37±0.95	87.12-92.02	-0.89±0.31	-1.67--0.09	8.91±1.97	5.53-13.26	86.78±1.97	83.10-89.96
1B/1R	N	85	51.20	0.97±0.30**	0.57-2.20	90.47±1.01*	87.12-92.16	-0.72±0.28**	-1.67--0.09	8.03±1.75**	5.21-13.26	87.45±1.85**	83.17-90.30
1B/1R	Y	81	48.80	1.39±0.44	0.57-2.96	90.15±0.84	88.34-92.02	-1.02±0.35	-1.78--0.09	9.67±2.01	5.33-14.69	86.11±1.88	81.36-90.08

表4

图1

表5

图2

表6

表7

表8

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性状 Trait	环境 Environment	平均值 Mean	标准差 Standard deviation	范围 Range	变异系数 Coefficient of variation (%)
黄色素含量 YPC (μg·g^-1)	2020济南2020JN	1.15	0.42	0.53-2.80	36.83
	2020德州2020DZ	1.19	0.45	0.51-2.76	37.69
	2021济南2021JN	1.09	0.41	0.52-3.06	37.56
	2021德州2021DZ	1.28	0.46	0.61-3.23	35.52
	平均Mean	1.18	0.43	0.57-2.96	36.36
L值 L value	2020济南2020JN	90.06	1.05	87.24-92.25	1.17
	2020德州2020DZ	90.02	1.07	86.69-92.06	1.18
	2021济南2021JN	90.45	1.05	87.66-92.61	1.16
	2021德州2021DZ	90.64	1.03	86.89-92.75	1.14
	平均Mean	90.29	0.97	87.12-92.16	1.08
a* a* value	2020济南2020JN	-0.78	0.34	-1.62-0.01	43.57
	2020德州2020DZ	-0.80	0.37	-1.71-0.14	46.08
	2021济南2021JN	-0.94	0.38	-2.12-0.07	40.45
	2021德州2021DZ	-0.94	0.38	-1.91-0.02	40.10
	平均Mean	-0.86	0.35	-1.78-0.09	40.64
b* b* value	2020济南2020JN	8.42	1.94	5.02-14.16	23.02
	2020德州2020DZ	8.51	1.95	5.17-13.70	22.95
	2021济南2021JN	9.14	2.28	5.46-15.76	24.96
	2021德州2021DZ	9.26	2.26	5.11-15.16	24.42
	平均Mean	8.83	2.04	5.21-14.69	23.15
白度 Whiteness	2020济南2020JN	86.90	1.90	81.84-90.37	2.18
	2020德州2020DZ	86.80	1.92	82.00-90.38	2.21
	2021济南2021JN	86.69	2.21	80.48-90.65	2.55
	2021德州2021DZ	86.75	2.21	81.08-90.73	2.54
	平均Mean	86.78	1.98	81.35-90.30	2.28

变异来源 Source of variation	自由度 Df	黄色素含量 YPC (μg·g^-1)		*L值 L* value**		*a值 a* value**		*b值 b* value**		白度 Whiteness
变异来源 Source of variation	自由度 Df	平方和 Sum of squares	F 值 F value	平方和 Sum of squares	F 值 F value	平方和 Sum of squares	F 值 F value	平方和 Sum of squares	F 值 F value	平方和 Sum of squares	F 值 F value
环境Environment	3	6.40	318.40**	88.18	299.04**	7.22	398.13**	176.15	567.65**	7.05	17.81**
重复Replicate	4	0.55	20.66**	2.14	5.44*	0.13	5.24*	2.25	5.43*	2.55	4.83*
基因型Genotype	165	238.31	215.66**	1230.80	75.89**	158.55	158.92**	5452.96	319.50**	5105.17	234.58**
基因型×环境Genotype×Environment	495	6.91	2.09**	196.77	4.04**	13.97	4.67**	378.18	7.39**	450.64	6.90**
误差Error	636	4.26		62.51		3.85		65.79		83.88

优异等位基因数目 Number of superior alleles	品种 Varieties (lines)
9	郑引1号、淄麦12 Zhengyin1, Zimai12
8	皖麦19、皖麦50、皖麦52、阿夫、临麦4号、烟农15 Wanmai19, Wanmai50, Wanmai52, Funo, Linmai4, Yannong15
7	矮丰3号、丰产3号、阜936、皖麦53、宿0663、宿农6号、良星99、烟农19、临麦2号、鲁麦23、鲁麦6号、石家庄15、小偃22、小偃81 Aifeng3, Fengchan3, Fu936, Wanmai53, Su0663, Sunong6, Liangxing99, Yannong19, Linmai2, Lumai23, Lumai6, Shijiazhuang15, Xiaoyan22, Xiaoyan81

优异等位基因数目 Number of superior alleles	品种数 Number of sample	黄色素含量 YPC (μg·g^-1)	*L值 L* value**	*a值 a* value**	*b值 b* value**	白度 Whiteness
1	4	1.95A	89.88C	-1.36D	11.65A	84.49D
2	10	1.34B	89.79C	-1.00C	10.01B	85.63CD
3	22	1.31B	90.06C	-0.96BC	9.53B	86.17C
4	44	1.32B	90.26BC	-0.97BC	9.26B	86.47C
5	37	1.11BC	90.22BC	-0.83ABC	8.79B	86.77C
6	27	1.03BC	90.28BC	-0.73ABC	8.32BC	87.16BC
7	14	0.85C	90.95AB	-0.64A	7.03CD	88.50AB
8	6	0.83C	91.23A	-0.67AB	6.68D	88.95A
9	2	0.83	90.87	-0.63	6.98	88.50

地区 Region	黄色素含量 YPC (μg·g^-1)	*L值 L* value**	*a值 a* value**	*b值 b* value**	白度 Whiteness
国外Foreign	1.33A	89.85BC	-0.87AB	9.56A	85.98B
中国安徽Anhui, China	1.01B	91.14A	-0.82AB	7.51B	88.32A
中国河北Hebei, China	0.99B	89.80C	-0.69A	8.77A	86.49B
中国河南Henan, China	1.29A	90.35BC	-0.95B	9.07A	86.68B
中国山东Shandong, China	1.19AB	90.42B	-0.94B	8.92A	86.83B
中国陕西Shaanxi, China	1.01B	90.13BC	-0.70A	8.44AB	86.94B

小麦面粉色泽相关位点的育种利用效应分析

Analysis of the Breeding Utilization Effects of Loci Related to Wheat Flour Color

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基因 Gene	等位基因 Allele	分布频率Distribution frequences (%)
基因 Gene	等位基因 Allele	总计 Total	国外 Foreign	中国安徽 Anhui, China	中国河北 Hebei, China	中国河南 Henan, China	中国山东 Shandong, China	中国陕西 Shaanxi, China
Psy-A1	a	65.06	90.91	26.67	42.86	81.48	72.73	40.00
Psy-A1	b	34.94	9.09	73.33	57.14	18.52	27.27	60.00
Pds-B1	a	79.52	90.91	80.00	78.57	83.33	66.67	80.00
Pds-B1	b	20.48	9.09	20.00	21.43	16.67	33.33	20.00
Lcye-A1	a	2.41	9.09	0.00	7.14	1.85	0.00	0.00
Lcye-A1	b	97.59	90.91	100.00	92.86	98.15	100.00	100.00
Lcye-B1	a	33.73	27.27	13.33	21.43	51.85	39.39	16.00
Lcye-B1	b	66.27	72.73	86.67	78.57	48.15	60.61	84.00
Lox-B1	a	26.38	9.09	53.33	7.14	22.64	51.61	16.00
Lox-B1	b	73.62	90.91	46.67	92.86	77.36	48.39	84.00
Ppo-A1	a	49.09	68.18	20.00	57.14	71.70	24.24	32.00
Ppo-A1	b	50.91	31.82	80.00	42.86	28.30	75.76	68.00
Ppo-D1	a	57.23	54.55	33.33	71.43	74.07	51.52	36.00
Ppo-D1	b	42.77	45.45	66.67	28.57	25.93	48.48	64.00
Pod-2D	AA	36.14	45.45	33.33	42.86	25.93	18.18	64.00
	GG	48.80	40.91	20.00	28.57	61.11	72.73	32.00
	AG	15.06	13.64	46.67	28.57	12.96	9.09	4.00
1B/1R	N	51.20	68.18	80.00	28.57	31.48	63.64	64.00
1B/1R	Y	48.80	31.82	20.00	71.43	68.52	36.36	36.00

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