葡萄铁蛋白基因Ferritin的鉴定、克隆及其在果实发育不同时期对氨基酸铁复合肥喷施的响应

doi:10.3864/j.issn.0578-1752.2023.18.011

中国农业科学 ›› 2023, Vol. 56 ›› Issue (18): 3629-3641.doi: 10.3864/j.issn.0578-1752.2023.18.011

葡萄铁蛋白基因Ferritin的鉴定、克隆及其在果实发育不同时期对氨基酸铁复合肥喷施的响应

宋志忠¹^,²^,³(), 王建萍¹^,², 史圣朋³^,⁴, 曹晶雯², 刘万好¹, 徐维华¹, 肖慧琳¹^,²(), 唐美玲¹^,²()

¹ 烟台农业科学研究院葡萄研究所，中国山东烟台 264000
² 鲁东大学农林工程研究院/山东省高等学校重点实验室（作物高产抗逆分子模块育种实验室），中国山东烟台 264025
³ 剑桥大学植物系，英国剑桥 CB2 3EA
⁴ 剑桥大学沃尔森学院，英国剑桥 CB3 9BB

收稿日期:2023-02-23 接受日期:2023-06-05 出版日期:2023-09-16 发布日期:2023-09-21
通信作者:
肖慧琳，Tel：0535-6352051；E-mail：1316959443@qq.com。
唐美玲，Tel：0535-6352051；E-mail：tmling1999@163.com
联系方式: 宋志忠，Tel：0535-6664662；E-mail：szhzh2000@163.com。
基金资助:
国家现代农业产业技术体系建设专项资金(CARS-29-17); 国家留学基金(202208370080); 山东省重点研发计划（重大科技创新工程）(2022CXGC010605); 烟台市科技计划(2020XCZX026)

Identification and Cloning of Ferritin Family Genes in Grape and Response to Compound Amino Acid-Iron Spraying During Different Fruit Developmental Stages

SONG ZhiZhong¹^,²^,³(), WANG JianPing¹^,², SHI ShengPeng³^,⁴, CAO JingWen², LIU WanHao¹, XU WeiHua¹, XIAO HuiLin¹^,²(), TANG MeiLing¹^,²()

¹ Institute of Grape, Yantai Academy of Agricultural Science, Yantai 264000, Shandong, China
² The Engineering Research Institute of Agriculture and Forestry, Universities of Shandong Ludong University/Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants, Yantai 264025, Shandong, China
³ Department of Plant Science, University of Cambridge, Cambridge CB2 3EA, United Kingdom
⁴ Wolfson College, University of Cambridge, Cambridge CB3 9BB, United Kingdom

Received:2023-02-23 Accepted:2023-06-05 Published:2023-09-16 Online:2023-09-21

摘要/Abstract

摘要：

【目的】铁蛋白Ferritin在植物生长发育过程中发挥重要作用，其在果树中的生物学功能尚未见报道。克隆葡萄Ferritin家族基因并揭示其在果实发育不同时期的表达模式及其对叶面喷施氨基酸-铁（Fe）复合肥处理的响应差异，可为研究果树铁素营养与代谢的分子机制提供理论依据。【方法】通过同源克隆法从‘马瑟兰’中克隆Ferritin家族基因，利用生物信息学手段分析葡萄Ferritin及其编码蛋白的详细特征；设置叶面喷施氨基酸-Fe复合肥处理，利用荧光实时定量PCR技术分析Ferritin在葡萄果实发育不同时期的表达特征及其对叶面喷施处理的差异响应。【结果】在葡萄基因组中检索并克隆获得4个Ferritin家族基因，命名为VvFer1—VvFer4，分布于第6、8和13号三条染色体上，均含有7个长度不一的内含子，且主要定位于叶绿体和细胞核。本文所选16种植物Ferritin蛋白序列的一致性高达61.48%，系统发育树分析表明同一属的Ferritin同源蛋白如十字花科的拟南芥和芜菁，茄科的烟草和马铃薯，豆科的大豆、落花生和鹰嘴豆，大戟科的橡胶树、木薯和蓖麻，蔷薇科的苹果、桃和草莓，遗传进化关系较近，葡萄VvFer3和茄科同源蛋白遗传关系最近。葡萄VvFer基因在五年生‘马瑟兰’果实发育不同时期、不同组织中的表达水平差异较大，其中，VvFer3在不同组织中的表达量均最高，最大值出现在硬核期至成熟期的果实中，其次是VvFer2和VvFer4。‘马瑟兰’葡萄发育不同时期果实中的Fe含量略有差异，从幼果期开始逐渐增加，并在转色期果实中达到最高值，从转色期到成熟期略有下降，但仍高于幼果期和硬核期果实中的Fe含量，叶面喷施处理显著提高了成熟期果实中的Fe含量、乌头酸酶ACO（aconitase）、硝酸还原酶NIR（nitrite reductase）和琥珀酸脱氢酶SDH（succinate dehydrogenase）的酶活性。VvFer2—4在转录水平易受叶面喷施铁肥的诱导而显著增强，且与葡萄组织/器官分布和果实发育的不同时期密切相关，VvFer2在葡萄发育整个周期仅在果实中对叶面喷施铁肥处理比较敏感，VvFer3在所有组织中仅从幼果期至转色期对叶面喷施铁肥有响应，VvFer4在葡萄发育整个周期的韧皮部和叶片中持续受叶面喷施铁肥的诱导，而在果实中仅从幼果期至转色期对叶面喷施铁肥有响应；VvFer1在本文所选组织中的表达量相对较低，但很均匀，且在转录水平对叶面喷施铁肥没有响应。【结论】从葡萄中克隆并鉴定了4个铁蛋白基因Ferritin，其在葡萄发育不同时期、不同组织中的表达水平差异较大，并在转录水平易受叶面喷施铁肥的诱导；VvFer3在葡萄所有组织中的整体表达量最高（特别是果实中最为突出），且在幼果期至转色期易受铁肥叶面喷施的调控。

关键词: 葡萄, Fe的储藏与封存, Fe蛋白, 氨基酸-Fe复合肥, 叶面喷施

Abstract:

【Objective】Ferritin plays an important role in plant growth and development, and its biological function in fruit trees are essentially unknown. Cloning of grape Ferritin family genes and revealing their expression patterns at different fruit developmental stages and their response differences to foliar spraying of amino acid iron (Fe) compound fertilizers could provide a theoretical basis for studying the molecular mechanisms of Fe nutrition and metabolism in fruit trees. 【Method】The Ferritin family genes were screened and identified in grape genome by homologous cloning. The detailed characteristics of Ferritin genes and their encoded proteins were analyzed by using bioinformatical methods. The tissue-specific expression patterns of Ferritin family genes during distinct fruit developmental stages and differential response to foliar spraying of amino acid-iron compound fertilizer were determined by carrying out real-time fluorescent quantitative PCR. 【Result】In total, 4 Ferritin family genes were retrieved and cloned from grape, entitled with VvFer1-VvFer4, which were distributed on No. 6, 8 and 13 chromosomes, containing 7 introns with different lengths. VvFer proteins were mainly located in chloroplast and nucleus. The amino acid sequence identity of Ferritin homologs from 16 plant species was as high as 61.48%. Phylogenetic tree analysis indicated that Ferritin homologs belonging to the same genus, such as Arabidopsis and turnip of Cruciferae, tobacco and potato of Solanaceae, soybeans, peanuts and chickpeas of Leguminosae, rubber trees, cassava and castor of Euphorbiaceae, and apples, peaches and strawberries of Rosaceae, possessed a closer genetic distance during evolution. Grape VvFer3 was closely clustered with Solanaceae homologs. The expression levels of VvFer genes were different among distinct tissues or organs of 5-year-old Mathelan grape trees during different fruit developmental stages. In particular, the expression level of VvFer3 was the most abundant, and the maximum expression was observed in fruits from hard core stage to mature stage, followed by VvFer2 and VvFer4. The content of Fe Marselan fruits was slightly different among distinct grape developmental stages, which was gradually increased from young fruit stage, and reached the highest value at veraison stage, and then slightly decreased until mature stage, but still higher than that of young fruit stage and hard core stage. Foliar spraying treatment significantly enhanced Fe content of fruits at mature stage, accompanied by ACO (aconitase), NIR (nitrate reductase) and SDH (succinate dehydrogenase). Genes of VvFer2-4 were significantly up-regulated by foliar spraying of amino acid-iron compound fertilizer, which was closely related to distinct grape tissues/organs and different fruit developmental stages. In details, the expression of VvFer2 in fruits was sensitive to foliar spraying treatment during the whole period of grape development. The expressions of VvFer3 in all tested tissues were sensitive to foliar spraying treatment from young fruit stage to veraison stage. The expressions of VvFer4 in phloem and leaves were continuously induced by foliar spraying treatment during the whole period of grape development, whereas in fruits from young fruit stage to veraison stage. The expression of VvFer1 was relatively low, but very uniform, and there was no response to foliar spraying treatment at the transcription level. 【Conclusion】Four Ferritin family genes were cloned and identified in grape, whose expression were significantly different among distinct tissues during different fruit developmental stages and were prone to be up-regulated under foliar spraying treatment of amino acid-iron compound fertilizer. The overall expression level of VvFer3 gene was the highest in all tested tissues (especially in fruits) during the whole fruit development stage, and was up-regulated in fruits under foliar spraying treatment from young fruit stage to the verason stage.

Key words: grape, Fe storage and sequestration, Ferritin, compound amino acid-iron fertilizer, foliar spraying

宋志忠, 王建萍, 史圣朋, 曹晶雯, 刘万好, 徐维华, 肖慧琳, 唐美玲. 葡萄铁蛋白基因Ferritin的鉴定、克隆及其在果实发育不同时期对氨基酸铁复合肥喷施的响应[J]. 中国农业科学, 2023, 56(18): 3629-3641.

SONG ZhiZhong, WANG JianPing, SHI ShengPeng, CAO JingWen, LIU WanHao, XU WeiHua, XIAO HuiLin, TANG MeiLing. Identification and Cloning of Ferritin Family Genes in Grape and Response to Compound Amino Acid-Iron Spraying During Different Fruit Developmental Stages[J]. Scientia Agricultura Sinica, 2023, 56(18): 3629-3641.

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参考文献 35

[1]	李俊成, 于慧, 杨素欣, 冯献忠. 植物对铁元素吸收的分子调控机制研究进展. 植物生理学报, 2016, 52(6): 835-842.
	LI J C, YU H, YANG S X, FENG X Z. Research progress of molecular regulation of iron uptake in plants. Plant Physiology Journal, 2016, 52(6): 835-842. (in Chinese)
[2]	BARTON L L, ABADIA J. Iron Nutrition in Plants and Rhizospheric Microorganisms. Dordrecht: Springer Netherlands, 2006.
[3]	LILL R. Function and biogenesis of iron-sulphur proteins. Nature, 2009, 460(7257): 831-838. doi: 10.1038/nature08301
[4]	JIMÉNEZ S, GOGORCENA Y, HÉVIN C, ROMBOLÀ A D, OLLAT N. Nitrogen nutrition influences some biochemical responses to iron deficiency in tolerant and sensitive genotypes of Vitis. Plant and Soil, 2007, 290(1/2): 343-355. doi: 10.1007/s11104-006-9166-4
[5]	PESTANA M, BEJA P, CORREIA P J, DE VARENNES A, FARIA E A. Relationships between nutrient composition of flowers and fruit quality in orange trees grown in calcareous soil. Tree Physiology, 2005, 25(6): 761-767. pmid: 15805096
[6]	TAGLIAVINI M, ABADÍA J, ROMBOLÀ A D, ABADÍA A, TSIPOURIDIS C, MARANGONI B. Agronomic means for the control of iron deficiency chlorosis in deciduous fruit trees. Journal of Plant Nutrition, 2000, 23(11/12): 2007-2022. doi: 10.1080/01904160009382161
[7]	KOBAYASHI T, NISHIZAWA N K. Iron uptake, translocation, and regulation in higher plants. Annual Review of Plant Biology, 2012, 63: 131-152. doi: 10.1146/annurev-arplant-042811-105522 pmid: 22404471
[8]	张妮娜, 上官周平, 陈娟. 植物应答缺铁胁迫的分子生理机制及其调控. 植物营养与肥料学报, 2018, 24(5): 1365-1377.
	ZHANG N N, SHANGGUAN Z P, CHEN J. Molecular physiological mechanism and regulation of plant responses to iron deficiency stress. Journal of Plant Nutrition and Fertilizer, 2018, 24(5): 1365-1377. (in Chinese)
[9]	ISHIMARU Y, SUZUKI M, TSUKAMOTO T, SUZUKI K, NAKAZONO M, KOBAYASHI T, WADA Y, WATANABE S, MATSUHASHI S, TAKAHASHI M, NAKANISHI H, MORI S, NISHIZAWA N K. Rice plants take up iron as an Fe³⁺-phytosiderophore and as Fe²⁺. The Plant Journal, 2006, 45(3): 335-346. doi: 10.1111/tpj.2006.45.issue-3
[10]	ZHANG X X, ZHANG D, SUN W, WANG T Z. The adaptive mechanism of plants to iron deficiency via iron uptake, transport, and homeostasis. International Journal of Molecular Sciences, 2019, 20(10): 2424. doi: 10.3390/ijms20102424
[11]	FOURCROY P, TISSOT N, GAYMARD F, BRIAT J F, DUBOS C. Facilitated Fe nutrition by phenolic compounds excreted by the Arabidopsis ABCG37/PDR9 transporter requires the IRT1/FRO2 high-affinity root Fe²⁺ transport system. Molecular Plant, 2016, 9(3): 485-488. doi: 10.1016/j.molp.2015.09.010
[12]	MONDAL S, PRAMANIK K, GHOSH S K, PAL P, GHOSH P K, GHOSH A, MAITI T K. Molecular insight into arsenic uptake, transport, phytotoxicity, and defense responses in plants: a critical review. Planta, 2022, 255(4): 87. doi: 10.1007/s00425-022-03869-4 pmid: 35303194
[13]	BRIAT J F, DUC C, RAVET K, GAYMARD F. Ferritins and iron storage in plants. Biochimica et Biophysica Acta (BBA)-General Subjects, 2010, 1800(8): 806-814. doi: 10.1016/j.bbagen.2009.12.003
[14]	LÓPEZ-MILLÁN A F, DUY D, PHILIPPAR K. Chloroplast iron transport proteins-Function and impact on plant physiology. Frontiers in Plant Science, 2016, 7: 178.
[15]	PETIT J M, BRIAT J F, LOBRÉAUX S. Structure and differential expression of the four members of the Arabidopsis thaliana ferritin gene family. The Biochemical Journal, 2001, 359: 575-582. doi: 10.1042/bj3590575
[16]	黄莹. FER2基因在拟南芥干旱胁迫响应中的功能研究[D]. 合肥: 合肥工业大学, 2018.
	HUANG Y. Function of FER2 gene in response to drought stress in Arabidopsis thaliana[D]. Hefei: Hefei University of Technology, 2018. (in Chinese)
[17]	LIU J T, FAN Y W, ZOU J, FANG Y Q, WANG L H, WANG M, JIANG X Q, LIU Y Q, GAO J P, ZHANG C Q. A RhABF2/Ferritin module affects rose (Rosa hybrida) petal dehydration tolerance and senescence by modulating iron levels. The Plant Journal, 2017, 92(6): 1157-1169. doi: 10.1111/tpj.2017.92.issue-6
[18]	RAVET K, TOURAINE B, BOUCHEREZ J, BRIAT J F, GAYMARD F, CELLIER F. Ferritins control interaction between iron homeostasis and oxidative stress in Arabidopsis. The Plant Journal, 2009, 57(3): 400-412. doi: 10.1111/tpj.2009.57.issue-3
[19]	REYT G, BOUDOUF S, BOUCHEREZ J, GAYMARD F, BRIAT J F. Iron- and ferritin-dependent reactive oxygen species distribution: impact on Arabidopsis root system architecture. Molecular Plant, 2015, 8(3): 439-453. doi: 10.1016/j.molp.2014.11.014
[20]	钟晨, 苏军, 汤婷婷, 丁伟, 朱立武, 贾兵. ‘砀山酥梨’叶片中Fer2基因的克隆与表达分析. 南京农业大学学报, 2013, 36(5): 33-38.
	ZHONG C, SU J, TANG T T, DING W, ZHU L W, JIA B. Cloning and differential expression analysis of Fer2 gene in leaf of ‘Dangshansuli’ pear. Journal of Nanjing Agricultural University, 2013, 36(5): 33-38. (in Chinese)
[21]	吕晨艳. 大豆铁蛋白吸收铁的途径及体外细胞吸收研究[D]. 北京: 中国农业大学, 2015.
	LÜ C Y. Study on the ways of iron absorption by soybean ferritin and its cell absorption in vitro[D]. Beijing: China Agricultural University, 2015. (in Chinese)
[22]	施富超. 木薯Ferritin基因功能分析[D]. 南昌: 南昌大学, 2018.
	SHI F C. Functional analysis of ferritin gene in cassava[D]. Nanchang: Nanchang University, 2018. (in Chinese)
[23]	JAILLON O, AURY J M, NOEL B, POLICRITI A, CLEPET C, CASAGRANDE A, CHOISNE N, AUBOURG S, VITULO N, JUBIN C, et al. The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature, 2007, 449(7161): 463-467. doi: 10.1038/nature06148
[24]	张璐, 宗亚奇, 徐维华, 韩蕾, 孙浈育, 陈朝晖, 陈松利, 张凯, 程杰山, 唐美玲, 张洪霞, 宋志忠. 葡萄Fe-S簇装配基因的鉴定、克隆和表达特征分析. 中国农业科学, 2021, 54(23): 5068-5082. doi: 10.3864/j.issn.0578-1752.2021.23.012. doi: 10.3864/j.issn.0578-1752.2021.23.012
	ZHANG L, ZONG Y Q, YU W H, HAN L, SUN Y Z, CHEN C H, CHEN S L, ZHANG K, CHENG J S, TANG M L, ZHANG H X, SONG Z Z. Identification, cloning and expression characteristics analysis of Fe-S cluster assembly genes in grape. Scientia Agricultura Sinica, 2021, 54(23): 5068-5082. doi: 10.3864/j.issn.0578-1752.2021.23.012. (in Chinese) doi: 10.3864/j.issn.0578-1752.2021.23.012
[25]	房经贵, 刘崇怀. 葡萄分子生物学. 北京: 科学出版社, 2014.
	FANG J G, LIU C H. Grape Molecular Biology. Beijing: Science Press, 2014. (in Chinese)
[26]	SONG Z Z, MA R J, ZHANG B B, GUO S L, YU M L, KORIR N K. Differential expression of iron-sulfur cluster biosynthesis genes during peach fruit development and ripening, and their response to iron compound spraying. Scientia Horticulturae, 2016, 207: 73-81. doi: 10.1016/j.scienta.2016.05.024
[27]	SHENG Y T, CHENG H, WANG L M, SHEN J Y, TANG M L, LIANG M X, ZHANG K, ZHANG H X, KONG Q, YU M L, SONG Z Z. Foliar spraying with compound amino acid-iron fertilizer increases leaf fresh weight, photosynthesis, and Fe-S cluster gene expression in peach (Prunus persica (L.) batsch). BioMed Research International, 2020, 2020: 2854795.
[28]	SONG Z Z, LIN S Z, FU J Y, CHEN Y H, ZHANG H X, LI J Z, LIANG M X. Heterologous expression of ISU1 gene from Fragaria vesca enhances plant tolerance to Fe depletion in Arabidopsis. Plant Physiology and Biochemistry, 2022, 184: 65-74. doi: 10.1016/j.plaphy.2022.05.010
[29]	SONG Z Z, YANG Y, XU J L, MA R J, YU M L. Physiological and transcriptional responses in the iron-sulphur cluster assembly pathway under abiotic stress in peach (Prunus persica L.) seedlings. Plant Cell, Tissue and Organ Culture, 2014, 117(3): 419-430. doi: 10.1007/s11240-014-0452-1
[30]	王壮伟, 王庆莲, 夏瑾, 王西成, 宋志忠, 吴伟民. 葡萄KEA家族基因的克隆、鉴定及表达分析. 中国农业科学, 2018, 51(23): 4522-4534. doi: 10.3864/j.issn.0578-1752.2018.23.011. doi: 10.3864/j.issn.0578-1752.2018.23.011
	WANG Z W, WANG Q L, XIA J, WANG X C, SONG Z Z, WU W M. Cloning, characterization and expression analysis of K+/H+ antiporter genes in grape. Scientia Agricultura Sinica, 2018, 51(23): 4522-4534. doi: 10.3864/j.issn.0578-1752.2018.23.011. (in Chinese) doi: 10.3864/j.issn.0578-1752.2018.23.011
[31]	沈静沅, 唐美玲, 杨庆山, 高雅超, 刘万好, 程杰山, 张洪霞, 宋志忠. 葡萄钾离子通道基因VviSKOR的克隆、表达及电生理功能. 中国农业科学, 2020, 53(15): 3158-3168. doi: 10.3864/j.issn.0578-1752.2020.15.015. doi: 10.3864/j.issn.0578-1752.2020.15.015
	SHEN J Y, TANG M L, YANG Q S, GAO Y C, LIU W H, CHENG J S, ZHANG H X, SONG Z Z. Cloning, expression and electrophysiological function analysis of potassium channel gene VviSKOR in grape. Scientia Agricultura Sinica, 2020, 53(15): 3158-3168. doi: 10.3864/j.issn.0578-1752.2020.15.015. (in Chinese) doi: 10.3864/j.issn.0578-1752.2020.15.015
[32]	DENG W K, WANG Y B, LIU Z X, CHENG H, XUE Y. HemI: a toolkit for illustrating heatmaps. PLoS ONE, 2014, 9(11): e111988. doi: 10.1371/journal.pone.0111988
[33]	JEONG J, MERKOVICH A, CLYNE M, CONNOLLY E L. Directing iron transport in dicots: regulation of iron acquisition and translocation. Current Opinion in Plant Biology, 2017, 39: 106-113. doi: S1369-5266(17)30029-8 pmid: 28689052
[34]	李志安, 王伯荪, 林永标, 曾友特. 植物营养转移研究进展. 武汉植物学研究, 2000, 18(3): 229-236.
	LI Z A, WANG B S, LIN Y B, ZENG Y T. A review of study on nutrient resorption of plant. Journal of Wuhan Botanical Research, 2000, 18(3): 229-236. (in Chinese)
[35]	车艳芳, 曹花平. 葡萄高效栽培技术. 石家庄: 河北科学技术出版社, 2014.
	CHE Y F, CAO H P. Efficient Cultivation Techniques of Grapes. Shijiazhuang: Hebei Science & Technology Press, 2014. (in Chinese)

基因 Gene	上游引物 Forward primer	下游引物 Reverse primer	产物长度 Product length (bp)
VvFer1	ATGGAACTCTCTCAAAGGGAG	TCATGCTGCACCACCCTCTTC	894
VvFer2	ATGCTTGTGGGAGGTGTTTCA	CTAAGACTGACCAAGAAAGAG	786
VvFer3	ATGCTTCTCAAAGCTGCTTCA	TCACGCAGCAACAACTCCTCCA	798
VvFer4	ATGCTGCTCAAGTCGTCTGCT	TCATGCAGCAACCCCATGATT	777

基因 Gene	上游引物 Forward primer	下游引物 Reverse primer	产物长度 Product length (bp)
VvFer1	GCTGAGAAGGGAGATGCATT	CATGTCCCTTTCCAACCCTT	217
VvFer2	GATCCCCAGTTGACAGATTT	CCACCCTCTTCGAGGAGCATTT	152
VvFer3	GCATTGTCACTGGAGAAGCT	GCAGCAACAACTCCTCCATT	230
VvFer4	CGATAATGATGAAAAGGGAG	CTTGATAGATTCCACCTGCT	175

基因 Gene	登录号 Accession No.	染色体定位 Chromosome location	内含子数目 Intron number	氨基酸 Amino acid	等电点 PI	跨膜区Transmembrane	总平均亲水性 GRAVY	不稳定性指数 Instability index
VvFer1	VIT_208s0058g00430	chr8: 9389844..9394079 reverse	7	298	5.82	3	-0.346	51.73 不稳定Unstable
VvFer2	VIT_208s0058g00410	chr8: 9346913..9351311 reverse	7	261	5.57	3	-0.407	53.87 不稳定Unstable
VvFer3	VIT_213s0067g01840	chr13: 1003921..1006908 2. reverse	7	265	5.77	1	-0.244	51.42 不稳定Unstable
VvFer4	VIT_206s0004g07160	chr6: 7932473..7935888 reverse	7	258	6.20	0	-0.394	39.41 稳定Stable

蛋白 Protein	细胞核 Nucleus	叶绿体 Chlorophyll	线粒体 Mitochondrial	细胞质 Cytosol
VvFer1	78.58%	14.28%	7.14%	-
VvFer2	57.14%	25.01%	10.71%	7.14%
VvFer3	14.28%	75.01%	10.71%	-
VvFer4	-	92.86%	7.14%	-

	对照 Control	叶面喷施 Foliar spraying
顺乌头酸酶活性 ACO activity (U/mg protein)	0.53±0.06	0.79±0.07**
亚硝酸还原酶 NiR activity (U/mg protein)	2.49±0.32	3.65±0.38**
琥珀酸脱氢酶 SDH activity (U/mg protein)	5.97±0.62	7.59±0.64*

葡萄铁蛋白基因Ferritin的鉴定、克隆及其在果实发育不同时期对氨基酸铁复合肥喷施的响应

Identification and Cloning of Ferritin Family Genes in Grape and Response to Compound Amino Acid-Iron Spraying During Different Fruit Developmental Stages

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