玉米ZmCCT10耐低氮功能研究

doi:10.3864/j.issn.0578-1752.2023.06.002

中国农业科学 ›› 2023, Vol. 56 ›› Issue (6): 1035-1044.doi: 10.3864/j.issn.0578-1752.2023.06.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

玉米ZmCCT10耐低氮功能研究

李懿璞¹^,²(), 童丽秀³(), 蔺雅楠¹^,², 苏治军¹^,², 包海柱¹^,², 王富贵²^,⁴, 刘剑²^,⁴, 屈佳伟¹^,², 胡树平²^,⁴, 孙继颖¹^,², 王志刚¹^,², 于晓芳¹^,²(), 徐明良³(), 高聚林¹^,²()

¹ 内蒙古农业大学农学院，呼和浩特 010018
² 内蒙古自治区高校寒旱区作物种质资源保护与利用工程研究中心，呼和浩特 010018
³ 中国农业大学农学院，北京 100193
⁴ 内蒙古农业大学职业技术学院，内蒙古包头 014109

收稿日期:2022-12-09 接受日期:2022-12-27 出版日期:2023-03-16 发布日期:2023-03-23
联系方式: 李懿璞，E-mail：liyipu1987@163.com。童丽秀，E-mail：lixiu_tong2016@163.com。李懿璞和童丽秀为同等贡献作者。
基金资助:
内蒙古自然科学基金(2020BS03036); 内蒙古自治区直属高校基本科研业务费项目(BR22-11-02); 内蒙古自治区高等学校科学研究项目(NJZY19048); 内蒙古农业大学高层次人才引进科研启动项目(NDYB2018-12)

Investigation of Low Nitrogen Tolerance of ZmCCT10 in Maize

LI YiPu¹^,²(), TONG LiXiu³(), LIN YaNan¹^,², SU ZhiJun¹^,², BAO HaiZhu¹^,², WANG FuGui²^,⁴, LIU Jian²^,⁴, QU JiaWei¹^,², HU ShuPing²^,⁴, SUN JiYing¹^,², WANG ZhiGang¹^,², YU XiaoFang¹^,²(), XU MingLiang³(), GAO JuLin¹^,²()

¹ Agricultural College, Inner Mongolia Agricultural University, Hohhot 010018
² Region Research Center for Conservation and Utilization of Crop Germplasm Resources in Cold and Arid Areas, Hohhot 010018
³ College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193
⁴ Vocational and Technical College of Inner Mongolia Agricultural University, Baotou 014109, Inner Mongolia

Received:2022-12-09 Accepted:2022-12-27 Published:2023-03-16 Online:2023-03-23

摘要/Abstract

摘要：

【目的】土壤氮素缺乏影响玉米的产量和品质，是中国玉米生产面临的重大问题。ZmCCT10编码转录因子，具有一因多效性，ZmCCT10是调控玉米生长发育和响应非生物胁迫重要的调节因子。解析玉米耐低氮的分子机制、聚合抗性基因，为培育耐低氮和氮高效玉米品种奠定基础。【方法】通过比较ZmCCT10近等基因系在低氮胁迫和完全营养水培条件下与耐低氮胁迫相关的性状，分析低氮胁迫后ZmCCT10的表达模式，选择ZmCCT10在近等基因系表达量差异最大的部位与时间点，进行转录组测序。发掘玉米ZmCCT10响应耐低氮反应的特征，探究其参与耐低氮反应的分子机制。【结果】在低氮胁迫条件下，ZmCCT10近等基因系Y331-ΔTE和Y331的根长性状、生物量、氮素生理指标显著差异。其中，ZmCCT10不带有转座子插入的单倍型Y331-ΔTE的总根长、主胚根长、侧根长均显著长于Y331；根干重、地上部干重、氮积累量、硝酸还原酶活性也显著高于Y331。在低氮胁迫后，ZmCCT10在根部和叶片的表达量均显著高于对照处理，且近等基因系间的表达量也具有显著差异，根部和叶片的表达模式也不同。胁迫处理3 h后，ZmCCT10在根部的表达量达到峰值，而在叶片的表达量随胁迫处理时间的增加而持续升高，在胁迫处理后6 h达到峰值。选取0.04 mmol·L^-1低氮胁迫处理3 h的根部样品进行转录组测序，生物学重复间的相关系数达0.9以上。GO富集分析表明，低氮胁迫后，参与胺化物合成过程和细胞氮化物代谢过程的基因在近等基因系间的表达量差异显著。结合差异基因的表达量和表达模式，筛选ZmCCT10调控参与玉米耐低氮反应的候选基因。经qRT-PCR验证，转录组测序获得的与耐低氮相关的ZmMPK5、ZmNS2等基因在近等基因系胁迫前后的表达量差异显著。【结论】ZmCCT10是一个以转录调控的方式参与玉米耐低氮反应的候选基因。

关键词: 玉米, ZmCCT10, 耐低氮, 转录因子, 转录组

Abstract:

【Objective】 The lack of soil nitrogen impacts the yield and quality of maize, which is a major problem of maize production in China. ZmCCT10 encodes the transcription factor, which is pleiotropic. ZmCCT10 is a very important co-factor regulating the growth, development and responding to abiotic stress of maize. The molecular mechanism of maize tolerance to low nitrogen is the basis for breeding maize varieties with low nitrogen tolerance and high nitrogen efficiency. 【Method】 In this study, we compared the traits those relate to low-nitrogen tolerance, expression pattern of ZmCCT10 and transcriptome results of ZmCCT10 near-isogenic lines under low-nitrogen stress and complete nutrient conditions. To analysis the characteristics of ZmCCT10 in response to low-nitrogen stress and the molecular mechanisms involved in low-nitrogen tolerance were explored. 【Result】 This study indicated that different alleles of ZmCCT10 showed significant differences in root length traits, biomass and nitrogen physiological traits under low nitrogen stress. The Y331-ΔTE haplotype without transposon insertion of ZmCCT10 had significantly longer total root length, main radicle length and lateral root length than Y331 after low nitrogen stress. What is more, root dry weight, shoot dry weight, nitrogen accumulation and nitrate reductase activity were also significantly higher than Y331. The expression levels of ZmCCT10 in roots and leaves were significantly higher than those in the control treatment. The expression level of ZmCCT10 in roots reached a peak at 3 hours after stress treatment. In leaves, the expression of ZmCCT10 continued to increase and peaked 6 hours after stress treatment. Root samples were collected under 0.04 mmol·L^-1 low nitrogen stress after 3h for transcriptome sequencing. The correlation coefficients between biological replicates are more than 0.9. GO enrichment analysis showed that the expression levels of amine synthesis process and cellular nitrogen compound catabolic process were significantly different in near-isogenic lines after low nitrogen stress. Combined with the amount and expression pattern of differential genes, ZmCCT10-regulated candidate genes involved in low-nitrogen tolerance were selected. qRT-PCR confirmed that the expression levels of ZmMPK5, ZmNS2 and other genes were significantly different after stress in near-isogenic lines. 【Conclusion】 ZmCCT10 is a candidate gene involved in low nitrogen tolerance in maize and it participates in the low-nitrogen tolerance response of maize as transcriptional regulation.

Key words: maize, ZmCCT10, low nitrogen resistance, transcription factor, transcriptome

李懿璞, 童丽秀, 蔺雅楠, 苏治军, 包海柱, 王富贵, 刘剑, 屈佳伟, 胡树平, 孙继颖, 王志刚, 于晓芳, 徐明良, 高聚林. 玉米ZmCCT10耐低氮功能研究[J]. 中国农业科学, 2023, 56(6): 1035-1044.

LI YiPu, TONG LiXiu, LIN YaNan, SU ZhiJun, BAO HaiZhu, WANG FuGui, LIU Jian, QU JiaWei, HU ShuPing, SUN JiYing, WANG ZhiGang, YU XiaoFang, XU MingLiang, GAO JuLin. Investigation of Low Nitrogen Tolerance of ZmCCT10 in Maize[J]. Scientia Agricultura Sinica, 2023, 56(6): 1035-1044.

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参考文献 32

[1]	王艳朋, 靳静晨, 汤继华, 胡彦民, 刘宗华. 作物氮素高效利用研究与现代农业. 中国农学通报, 2007, 23(10): 179-183.
	WANG Y P, JIN J C, TANG J H, HU Y M, LIU Z H. Research on the high nitrogen use efficiency of crops and modern agriculture. Chinese Agricultural Science Bulletin, 2007, 23(10): 179-183. (in Chinese)
[2]	CHEN F J, FANG Z G, GAO Q, YE Y L, JIA L L, YUAN L X, MI G H, ZHANG F S. Evaluation of the yield and nitrogen use efficiency of the dominant maize hybrids grown in North and Northeast China. Science China Life Sciences, 2013, 56(6): 552-560. doi: 10.1007/s11427-013-4462-8 pmid: 23504275
[3]	GALLOWAY J N, TOWNSEND A R, ERISMAN J W, BEKUNDA M, CAI Z C, FRENEY J R, MARTINELLI L A, SEITZINGER S P, SUTTON M A. Transformation of the nitrogen cycle: Recent trends, questions, and potential solutions. Science, 2008, 320(5878): 889-892. doi: 10.1126/science.1136674 pmid: 18487183
[4]	ROBSON F, COSTA M M, HEPWORTH S R, VIZIR I, PIÑEIRO M, REEVES P H, PUTTERILL J, COUPLAND G. Functional importance of conserved domains in the flowering-time gene CONSTANS demonstrated by analysis of mutant alleles and transgenic plants. The Plant Journal, 2001, 28(6): 619-631. doi: 10.1046/j.1365-313x.2001.01163.x
[5]	LI Y P, XU M L. CCT family genes in cereal crops: a current overview. The Crop Journal, 2017, 5(6): 449-458. doi: 10.1016/j.cj.2017.07.001
[6]	LIU H Y, ZHOU X C, LI Q P, WANG L, XING Y Z. CCT domain-containing genes in cereal crops: Flowering time and beyond. Theoretical and Applied Genetics, 2020, 133(5): 1385-1396. doi: 10.1007/s00122-020-03554-8 pmid: 32006055
[7]	HUNG H Y, SHANNON L M, TIAN F, BRADBURY P J, CHEN C, FLINT-GARCIA S A, MCMULLEN M D, WARE D, BUCKLER E S, DOEBLEY J F, HOLLAND J B. ZmCCT and the genetic basis of day-length adaptation underlying the postdomestication spread of maize. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(28): E1913-E1921.
[8]	YANG Q, LI Z, LI W Q, KU L X, WANG C, YE J R, LI K, YANG N, LI Y P, ZHONG T, LI J S, CHEN Y H, YAN J B, YANG X H, XU M L. CACTA-like transposable element in ZmCCT attenuated photoperiod sensitivity and accelerated the postdomestication spread of maize. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(42): 16969-16974.
[9]	WANG C, YANG Q, WANG W X, LI Y P, GUO Y L, ZHANG D F, MA X N, SONG W, ZHAO, J R, XU M L. A transposon-directed epigenetic change in ZmCCT underlies quantitative resistance to Gibberella stalk rot in maize. New Phytologist, 2017, 215(4): 1503-1515. doi: 10.1111/nph.2017.215.issue-4
[10]	LI Y P, TONG L X, DENG L L, LIU Q Y, XING Y X, WANG C, LIU B S, YANG X H, XU M L. Evaluation of ZmCCT haplotypes for genetic improvement of maize hybrids. Theoretical and Applied Genetics, 2017, 130(12): 2587-2600. doi: 10.1007/s00122-017-2978-1
[11]	XU G H, WANG X F, HUANG C, XU D Y, LI D, TIAN J G, CHEN Q Y, WANG C L, LIANG Y M, WU Y Y. Complex genetic architecture underlies maize tassel domestication. New Phytologist, 2017, 214(2): 852-864. doi: 10.1111/nph.14400 pmid: 28067953
[12]	LI B, WANG Z, JIANG H, LUO J H, GUO T, TIAN F, ROSSI V, HE Y. ZmCCT10-relayed photoperiod sensitivity regulates natural variation in the arithmetical formation of male germinal cells in maize. New Phytologist, 2023, 237(2): 585-600. doi: 10.1111/nph.v237.2
[13]	ZHONG S Y, LIU H Q, LI Y, LIN Z W. Opposite response of maize ZmCCT to photoperiod due to transposon jumping. Theoretical and Applied Genetics, 2021, 134(9): 2841-2855. doi: 10.1007/s00122-021-03862-7
[14]	TONG L X, YAN M Z, ZHU M, YANG J, LI Y P, XU M L. ZmCCT haplotype H5 improves yield, stalk-rot resistance, and drought to tolerance in maize. Frontiers in Plant Science, 2022, 13: 984527. doi: 10.3389/fpls.2022.984527
[15]	SU H H, LIANG J C, ABOU-ELWAFA S F, CHENG H Y, DOU D D, REN Z Z, XIE J R, CHEN Z H, GAO, F R, KU L X, CHEN Y H. ZmCCT regulates photoperiod-dependent flowering and response to stresses in maize. BMC Plant Biology, 2021, 21(1): 453-467. doi: 10.1186/s12870-021-03231-y
[16]	ZHANG Z H, ZHANG X, LIN Z L, WANG J, XU M L, LAI J S, YU J, LIN Z W. The genetic architecture of nodal root number in maize. The Plant Journal, 2018, 93(6): 1032-1044. doi: 10.1111/tpj.13828 pmid: 29364547
[17]	于洋. 不同氮效率玉米对供氮的响应特征及转录组蛋白质组分析[D]. 哈尔滨: 东北农业大学, 2021.
	YU Y. Response characteristics and transcriptome proteome analysis of maize with different nitrogen efficiency to nitrogen supply[D]. Harbin: Northeast Agricultural University, 2021. (in Chinese)
[18]	储成才, 王毅, 王二涛. 植物氮磷钾养分高效利用研究现状与展望. 中国科学: 生命科学, 2021, 51(10): 1415-1423.
	CHU C C, WANG Y, WANG E T. Improving the utilization efficiency of nitrogen, phosphorus and potassium: current situation and future perspectives. Scientia Sinica (Vitae), 2021, 51(10): 1415-1423. (in Chinese)
[19]	MA F F, NI L, LIU L B, LI X, ZHANG H, ZHANG A Y, TAN M P, JIANG, M Y. ZmABA2, an interacting protein of Zm MPK5, is involved in abscisic acid biosynthesis and functions. Plant Biotechnology Journal, 2016, 14(2): 771-782. doi: 10.1111/pbi.2016.14.issue-2
[20]	YOON Y, SEO D H, SHIN H, KIM H J, KIM C M, JANG G. The role of stress-responsive transcription factors in modulating abiotic stress tolerance in plants. Agronomy, 2020, 10(6): 788. doi: 10.3390/agronomy10060788
[21]	ZHANG X Y, JIA H Y, LI T, WU J Z, NAGARAJAN R, LEI L, POWERS C, KAN C C, HUA W, LIU Z Y, CHEN C, CARVER B F, YAN L L. TaCol-B5 modifies spike architecture and enhances grain yield in wheat. Science, 2022, 376(6589): 180-183. doi: 10.1126/science.abm0717
[22]	XUE W Y, XING, Y Z, WENG X Y, ZHAO Y, TANG W J, WANG L, ZHOU H J, YU S B, XU C G, LI X H, ZHANG Q F. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nature Genetics, 2008, 40(6): 761-767. doi: 10.1038/ng.143
[23]	WENG X Y, WANG L, WANG J, HU Y, DU H, XU C G, XING Y Z, LI X H, XIAO J H, ZHANG Q F. Grain number, plant height, and heading date7 is a central regulator of growth, development, and stress response. Plant Physiology, 2014, 164(2): 735-747. doi: 10.1104/pp.113.231308 pmid: 24390391
[24]	WANG Q, SU Q, NIAN J, ZHANG J, GUO M, DONG G G, HU J, WANG R S, WEI C S, LI G W, WANG W, GUO H S, LIN S Y, QIAN W F, XIE X Z, QIAN Q, Chen F, ZUO J Z. The Ghd7 transcription factor represses ARE1 expression to enhance nitrogen utilization and grain yield in rice. Molecular Plant, 2021, 14(6): 1012-1023. doi: 10.1016/j.molp.2021.04.012
[25]	李鹏程. 玉米根系性状的遗传分析及其与氮效率的关系研究[D]. 北京: 中国农业大学, 2015.
	LI P C. Genetic analysis of root traits and the relationship with nitrogen use efficiency in maize (Zea mays L.)[D]. Beijing: China Agricultural University, 2015. (in Chinese)
[26]	HAWKESFORD M J, GRIFFITHS S. Exploiting genetic variation in nitrogen use efficiency for cereal crop improvement. Current Opinion in Plant Biology, 2019, 49: 35-42. doi: S1369-5266(18)30115-8 pmid: 31176099
[27]	赵志鑫. 陕A群、陕B群选育玉米自交系耐低氮特性评价[D]. 西安: 西北农林科技大学, 2020.
	ZHAO Z X. Evaluation of low nitrogen tolerance for maize inbred lines selected Shaan A and Shaan B groups[D]. Xi’an: Northwest A＆F University, 2020. (in Chinese)
[28]	陈范骏, 米国华, 张福锁, 王艳, 刘向生, 春亮. 华北区部分主栽玉米杂交种的氮效率分析. 玉米科学, 2003, 11(2): 78-82.
	CHEN F J, MI G H, ZHANG F S, WANG Y, LIU X S, CHUN L. Nitrogen use efficiency in some of main maize hybrids grown in North China. Journal of Maize Sciences, 2003, 11(2): 78-82. (in Chinese)
[29]	吴雅薇, 蒲玮, 赵波, 魏桂, 孔凡磊, 袁继超. 不同耐低氮性玉米品种的花后碳氮积累与转运特征. 作物学报, 2021, 47(5): 915-928. doi: 10.3724/SP.J.1006.2021.03033
	WU Y W, PU W, ZHAO B, WEI G, KONG F L, YUAN J C. Characteristics of post-anthesis carbon and nitrogen accumulation and translocation in maize cultivars with different low nitrogen tolerance. Acta Agronomica Sinica, 2021, 47(5): 915-928. (in Chinese) doi: 10.3724/SP.J.1006.2021.03033
[30]	FIXEN P, BRENTRUP F, BRUULSEMA T, GARCIA F, NORTON R, ZINGORE S. Nutrient/ fertilizer use efficiency: measurement, current situation and trends. Managing Water and Fertilizer for Sustainable Agricultural Intensification, 2015, 270: 8-38.
[31]	崔文芳, 高聚林, 于晓芳, 胡树平, 苏治军, 王志刚, 孙继颖, 谢岷. 氮高效玉米自交系的筛选指标及其子粒氮素营养特性分析. 植物营养与肥料学报, 2014, 20(2): 290-297.
	CUI W F, GAO J L, YU X F, HU S P, SU Z J, WANG Z G, SUN J Y, XIE M. The index for the screening of N-efficient inbred lines of maize and their N nutrition peculiarity in seed production. Journal of Plant Nutrition and Fertilizer, 2014, 20(2): 290-297. (in Chinese)
[32]	米国华. 论作物养分效率及其遗传改良. 植物营养与肥料学报, 2017, 23(6): 1525-1535.
	MI G H. Nutrient use efficiency in crops and its genetic improvement. Journal of Plant Nutrition and Fertilizer, 2017, 23(6): 1525-1535. (in Chinese)

引物名称Primer name	序列Sequence (5′-3′)
ZmCCT10_qRTPCR-F	AACGACGACGACCTCATCAG
ZmCCT10_qRTPCR-R	ACTGGAACTCGTGCAGGCAC
ZmMPK5_qRTPCR-F	ACTGATGGACCGCAAACC
ZmMPK5_qRTPCR-R	GGGTGACGAGGAAGTTGG
Zmb-ZIP44_qRTPCR-F	TCGATCGTTAACCAGCACGG
Zmb-ZIP44_qRTPCR-R	GTTCGACTCCTTGCGCTTG
ZmNS3_qRTPCR-F	CTGTTCACGGACCTCGTCAC
ZmNS3_qRTPCR-R	GGTAGTTGCCGAAGTAGGGG
ZmNS2_qRTPCR-F	TGCTTGCCGTGTACCACC
ZmNS2_qRTPCR-R	CTTTTGGTGGAGCGTGTTCG
GAPDH-F	ATCAACGGCTTCGGAAGGAT
GAPDH-R	CCGTGGACGGTGTCGTACTT

玉米ZmCCT10耐低氮功能研究

Investigation of Low Nitrogen Tolerance of ZmCCT10 in Maize

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