TETs与细胞程序性死亡相关基因在山羊妊娠早期输卵管及子宫角的表达

doi:10.3864/j.issn.0578-1752.2021.04.015

中国农业科学 ›› 2021, Vol. 54 ›› Issue (4): 845-854.doi: 10.3864/j.issn.0578-1752.2021.04.015

• 畜牧·兽医·资源昆虫 • 上一篇下一篇

TETs与细胞程序性死亡相关基因在山羊妊娠早期输卵管及子宫角的表达

赵乐(),杨海丽,李佳璐,杨永恒,张蓉,程文强,成磊,赵永聚()

西南大学动物科学技术学院/重庆市牧草与草食家畜重点实验室/重庆市草食动物资源保护与利用工程技术研究中心,重庆 400715

收稿日期:2020-03-12 接受日期:2020-07-10 出版日期:2021-02-16 发布日期:2021-02-16
通讯作者: 赵永聚
作者简介:赵乐,E-mail： zhaole0228@163.com。
基金资助:
国家自然科学基金(31772564);国家重点研发计划(2018YFD0502000);重庆市自然科学基金重点项目(cstc 2020 jcyj-zdxm X0015);重庆市研究生科研创新项目(CYS19108);重庆市现代山地特色高效农业产业技术体系草食牲畜,2020【11】

Expression Patterns of TETs and Programmed Cell Death Related Genes in Oviduct and Uterus of Early Pregnancy Goats

ZHAO Le(),YANG HaiLi,LI JiaLu,YANG YongHeng,ZHANG Rong,CHENG WenQiang,CHENG Lei,ZHAO YongJu()

College of Animal Science and Technology, Southwest University/Chongqing Key Laboratory of Forage & Herbivore/Chongqing Engineering Research Center for Herbivores Resource Protection and Utilization, Chongqing 400715

Received:2020-03-12 Accepted:2020-07-10 Online:2021-02-16 Published:2021-02-16
Contact: YongJu ZHAO

摘要/Abstract

摘要：

【目的】TET（ten-eleven translocation）家族蛋白在调控胚胎和胎盘发育等过程中具有重要作用,输卵管与子宫内膜细胞的死亡平衡会影响受精卵及早期胚胎的发育。因此探讨TETs、细胞程序性死亡相关基因和附植相关基因在妊娠早期山羊输卵管及子宫角中的表达规律及其潜在调控作用,以期为研究胚胎发育及附植的分子机制奠定基础。【方法】以合川白山羊为研究对象,采集妊娠19d（P19）山羊子宫角及输卵管组织,以空配19d（C19）为对照,利用H.E染色观察C19组和P19组山羊子宫角组织形态,利用qRT-PCR技术检测了胚胎附植相关基因（WNT5a、OPN、VEGFA）、TETs（TET1、TET2、TET3）、细胞程序性死亡相关基因（凋亡相关基因（BAX、BCL2、Caspase9）、焦亡相关基因（GSDMD、NLRP3、Caspase1）和炎症相关基因（NF-kB、TNF-α））的相对表达,并分析了TETs及细胞程序性死亡相关基因在输卵管和子宫角组织的表达相关性。【结果】H.E染色结果显示,在P19组中山羊子宫角的腺体增多,形态多变。qRT-PCR结果显示,OPN在P19组山羊子宫角中的表达量极显著高于C19组（P＜0.01）,BAX/BCL2的表达量显著增加（P＜0.05）,而WNT5a和 VEGFA表达差异不显著;TET1、TET2、TET3在山羊子宫角及输卵管中均有表达,在C19组和P19组山羊子宫角中TET1及TET2的表达量极显著高于C19组和P19组中TET3的表达（P＜0.01）;在P19组山羊输卵管中TET2（P＜0.01）、NF-kB（P＜0.01）、Caspase1（P＜0.01）、GSDMD（P＜0.05）显著增高。相关性分析表明,输卵管中TET2与TET3（P＜0.01）、NF-kB（P＜0.01）、Caspase9（P＜0.05）显著正相关;子宫角中,TET3与VEGFA（P＜0.05）、WNT5a（P＜0.01）显著负相关,胚胎附植关键基因OPN与Caspase1（P＜0.01）、NLRP3（P＜0.05）、GSDMD（P＜0.05）显著正相关,而细胞焦亡关键基因GSDMD在输卵管与子宫角中均与Bax（P＜0.05）、Caspase1（P＜0.01）、NLRP3（P＜0.05）呈显著正相关。【结论】在妊娠早期山羊子宫角和输卵管中TETs表达与细胞程序性死亡部分基因表达具有一定的相关性,推测TETs与细胞凋亡或焦亡的发生对输卵管、受精卵、早期胚胎及子宫的发育具有一定的调控作用。胚胎附植关键基因OPN与焦亡相关基因显著相关,暗示妊娠早期子宫角中细胞焦亡的发生可能参与胚胎附植。为研究TETs通过细胞程序性死亡相关基因在妊娠早期输卵管及子宫角中的表达规律提供了理论参考。

关键词: 山羊, 子宫角, 输卵管, 细胞程序性死亡, 基因表达

Abstract:

【Objective】The ten-eleven translocation (TET) protein family play crucial roles in embryo and placental development. The balance of cell death between oviduct and endometrial would affect the development of gametes and early embryos. In this study, the expression patterns and their potential regulatory role of TETs, programmed cell death related genes and blastocyst implantation-related genes in oviduct and uterus of goats in early pregnancy were mainly explored, so as to provide a basis for studying development of gametes and early embryos.【Method】In this study, the uterus and oviduct of Hechuan white goats during non-pregnant 19 days (C19) and pregnant 19 days (P19) were collected, and then the morphology uterine horn was showed by HE staining. Then, the genes, including blastocyst implantation-related genes (Wnt5a, OPN, VEGFA), TET family genes (TET1, TET2, TET3), programmed cell death-related genes (BAX, BCL2, Caspase9, GSDMD, NLRP3 and Caspase1) and inflammation-related genes (NF-kB、TNF-α) were detected by qRT-PCR. Finally, the correlation between TETs and programmed cell death-related genes expression were analyzed.【Result】The results showed that the gland number increased, and its morphology varied in uterine horn in P19 group. Compared with C19 group, OPN gene expression and Bax/BCL2 in uterine horn tissue were significantly increased in P19 group (P＜0.01), however, there was no significant difference between Wnt5a and VEGFA. TET family genes were expressed in both oviduct and uterus. The expression of TET1 and TET2 was significantly higher than TET3 in uterine horn (P＜0.01). TET2 (P＜0.01), NF-kB (P＜0.01), Caspase1 (P＜0.01), and GSDMD (P＜0.05) gene were significantly higher in P19 group of oviduct tissue. Correlation analysis showed that the relationship between TET2 and TET3 (P＜0.01), NF-kB (P＜0.01) and Caspase9 (P＜0.01) in oviduct tissue were positive correlation. The TET3 were negative correlated with VEGFA (P＜0.05) and WNT5a (P＜0.01). Blastocyst implantation-related genes OPN was positively correlated with Caspase1 (P＜0.01), NLRP3 (P＜0.05) and GSDMD (P＜0.05) in uterine horn. The GSDMD was positively correlated with Bax (P＜0.05), Caspase1 (P＜0.01) and NLRP3 (P＜0.05) in oviduct and uterus horn.【Conclusion】It was indicated that there was correlation between TETs and programmed cell death related genes. So, it was speculated that TET family genes and programmed cell death related genes played a key role in regulating the development of oviduct, zygote, early embryo and uterus. The key genes for embryo implantation of OPN were significantly correlated with pyroptosis related genes, suggesting that proptosis had a certain influence on the attachment of early embryos. These finding provided heretical references for studying the effect of TETs family genes and programmed cell death related genes on oviduct and uterine horn of early pregnancy.

Key words: goat, uterine horn, oviduct, programmed cell death, gene expression

赵乐,杨海丽,李佳璐,杨永恒,张蓉,程文强,成磊,赵永聚. TETs与细胞程序性死亡相关基因在山羊妊娠早期输卵管及子宫角的表达[J]. 中国农业科学, 2021, 54(4): 845-854.

ZHAO Le,YANG HaiLi,LI JiaLu,YANG YongHeng,ZHANG Rong,CHENG WenQiang,CHENG Lei,ZHAO YongJu. Expression Patterns of TETs and Programmed Cell Death Related Genes in Oviduct and Uterus of Early Pregnancy Goats[J]. Scientia Agricultura Sinica, 2021, 54(4): 845-854.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2021.04.015

https://www.chinaagrisci.com/CN/Y2021/V54/I4/845

图/表 10

表1

表2

图1

图2

图3

图4

图5

图6

表3

表4

参考文献 32

[1]	GUILLOMOT M. Cellular interactions during implantation in domestic ruminants. Journal of Reproduction and Fertility, 1995,49:39-51.
[2]	GUILLOMOT M, REINAUD P, LA BONNARDIERE C, CHARPIGNY G. Characterization of conceptus-produced goat interferon tau and analysis of its temporal and cellular distribution during early pregnancy. Journal of Reproduction and Fertility, 1998,112(1):149-156.
[3]	BAZER F W, SONG G, THATCHER W W. Roles of conceptus secretory proteins in establishment and maintenance of pregnancy in ruminants. Asian-Australasian Journal of Animal Sciences, 2012,25(1):1-16.
[4]	SUZUKI T, SAKUMOTO R, HAYASHI K G, OGISO T, KUNII H, SHIROZU T, KIM S W, BAI H, KAWAHARA M, KIMURA K, TAKAHASHI M. Involvement of interferon-tau in the induction of apoptotic, pyroptotic, and autophagic cell death-related signaling pathways in the bovine uterine endometrium during early pregnancy. The Journal of Reproduction and Development, 2018,64(6):495-502.
[5]	MA M, ZHOU Q J, XIONG Y, LI B, LI X T. Preeclampsia is associated with hypermethylation of IGF-1 promoter mediated by DNMT1. American Journal of Translational Research, 2018,10(1):16-39.
[6]	YAMAGUCHI S, SHEN L, LIU Y, SENDLER D, ZHANG Y. Role of Tet1 in erasure of genomic imprinting. Nature, 2013,504(7480):460-464.
[7]	ROCA F J, LOOMANS H A, WITTMAN A T, CREIGHTON C J, HAWKINS S M. Ten-Eleven translocation genes are downregulated in endometriosis. Current Molecular Medicine, 2016,16(3):288-298.
[8]	谭强, 罗南剑, 张艳丽, 安炳星, 陈秋羊, 赵永聚. 山羊早期胎儿组织TET1与Wnt通路基因的表达变化及其相关性. 中国农业科学, 2017,50(14):2816-2825.
	TAN Q, LUO N J, ZHANG Y L, AN B X, CHEN Q Y, ZHAO Y J. Expression patterns and correlation of Wnts and TET1 genes in early fetal tissues of Dazu Black Goat. Scientia Agricultura Sinica, 2017,50(14):2816-2825. (in Chinese)
[9]	LI C Y, DONG L, SU R, BI Y, QING Y, DENG X L, ZHOU Y L, HU C, YU M X, HUANG H, et al. Homoharringtonine exhibits potent anti-tumor effect and modulates DNA epigenome in acute myeloid leukemia by targeting SP1/TET1/5hmC. Haematologica, 2020,105(1): doi: 10.3324/haematol.2018.208835.
[10]	LI C, WANG F, WANG H. Tetrachloro-1, 4-benzoquinone induces apoptosis of mouse embryonic stem cells. Journal of Environmental Sciences (China), 2017,51:5-12.
[11]	ZHAO J, MA X L, MA J X, SUN L, LU B, WANG Y, XING G S, WANG Y, DONG B C, XU L Y, KUANG M J, FU L, BAI H H, MA Y, JIN W L. TET3 Mediates Alterations in the Epigenetic Marker 5hmC and Akt pathway in Steroid-Associated Osteonecrosis. The Official Journal of The American Society for Bone and Mineral Research, 2017,32(2):319-332.
[12]	SANT D W, MUSTAFI S, GUSTAFSON C B, CHEN J, SLINGERLAND J M, WANG G. Vitamin C promotes apoptosis in breast cancer cells by increasing TRAIL expression. Scientific Reports, 2018,8(1):5306.
[13]	潘少容, 曾克武, 白云. 细胞焦亡研究进展. 生理科学进展, 2019,50(2):135-140.
	PAN S R, ZENG K W, BAI Y. Recent research progress of pyroptosis. Progress in Physiological Sciences, 2019,50(2):135-140. (in Chinese)
[14]	GROEBNER A E, SCHULKE K, UNTERSEER S, REICHENBACH H D, REICHENBACH M, BUTTNER M, WOLF E, MEYER H H, ULBRICH S E. Enhanced proapoptotic gene expression of XAF1, CASP8 and TNFSF10 in the bovine endometrium during early pregnancy is not correlated with augmented apoptosis. Placenta, 2010,31(3):168-177.
[15]	WEI X, XIAOLING Z, KAI M, RUI W, JING X, MIN G, ZHONGHONG W, JIANHUI T, XINYU Z, LEI A. Characterization and comparative analyses of transcriptomes for in vivo and in vitro produced peri-implantation conceptuses and endometria from sheep. The Journal of Reproduction and Development, 2016,62(3):279-287.
[16]	TASSELL W, SLATER M, BARDEN J A, MURPHY C R. Endometrial cell death during early pregnancy in the rat. The Histochemical Journal, 2000,32(6):373-379.
[17]	BOEDDEKER S J, HESS A P. The role of apoptosis in human embryo implantation. Journal of Reproductive Immunology, 2015,108:114-122.
[18]	JAISWAL M K, AGRAWAL V, MALLERS T, GILMAN-SACHS A, HIRSCH E, BEAMAN K D. Regulation of apoptosis and innate immune stimuli in inflammation-induced preterm labor. Journal of Immunology, 2013,191(11):5702-5713.
[19]	段利利, 刘子冬, 王林, 马斌芳, 范忆冰, 徐莹, 郭芬芬. C1q和肿瘤坏死因子相关蛋白4(CTRP4)降低子痫前期大鼠滋养层细胞的caspase-1和IL-1β水平. 细胞与分子免疫学杂志, 2016,32:1441-1445.
	DUAN L L, LIU Z D, WANG L, MA B F, FAN Y B, XU Y, GUO F F. C1q and tumor necrosis factor related protein 4 (CTRP4) suppresses caspase-1/IL-1β inflammatory pathway in trophoblasts of rat models with preeclampsia. Chinese Journal of Cellular and Molecular Immunology, 2016,32:1441-1445. (in Chinese)
[20]	GOMEZ-LOPEZ N, ROMERO R, TARCA A L, MILLER D, PANAITESCU B, SCHWENKEL G, GUDICHA D W, HASSAN S S, PACORA P, JUNG E, HSU C D. Gasdermin D: Evidence of pyroptosis in spontaneous preterm labor with sterile intra-amniotic inflammation or intra-amniotic infection. American Journal of Reproductive Immunology, 2019,82(6):e13184.
[21]	杨雪, 郑文亚, 郑路程, 王芹芹, 熊芳颖, 刘犇. BDNF和TRKB蛋白在妊娠期山羊子宫和胎盘的表达. 中国畜牧杂志, 2018,54:75-78.
	YANG X, ZHENG W Y, ZHENG L C, WANG Q Q, XIONG F Y, LIU B. The expression of BDNF and TrkB in uterus and placenta of pregnant goats. Chinese Journal of Animal Science, 2018,54(06):75-78.
[22]	WANG X B, QI Q R, WU K L, XIE Q Z. Role of osteopontin in decidualization and pregnancy success. Reproduction, 2018,155(5):423-432.
[23]	JOYCE M M, GONZALEZ J F, LEWIS S, WOLDESENBET S, BURGHARDT R C, NEWTON G R, JOHNSON G A. Caprine uterine and placental osteopontin expression is distinct among epitheliochorial implanting species. Placenta, 2005,26(2-3):160-170.
[24]	唐毅平. 山羊胎盘滋养层细胞TET1基因对Wnt通路相关分子表达的影响[D]. 重庆: 西南大学, 2018.
	TANG Y P. The Effect of TET1 gene on the expression of Wnt pathway related molecules in trophoblast cells of goat (Capra Hircus)[D]. Chongqing: Southwest University, 2018. (in Chinese)
[25]	高静. 山羊胎盘滋养层细胞TET1调控MMPs和TIMPs分子的研究[D]. 重庆: 西南大学, 2019.
	GAO J. The research of TET1 regulated MMPs and TIMPs on goat trophoblast cells[D]. Chongqing: Southwest University, 2019. (in Chinese)
[26]	O'DOHERTY A M, O'SHEA L C, SANDRA O, LONERGAN P, FAIR T, FORDE N. Imprinted and DNA methyltransferase gene expression in the endometrium during the pre- and peri-implantation period in cattle. Reproduction, Fertility,and Development, 2017,29(9):1729-1738.
[27]	ZGLEJC-WASZAK K, WASZKIEWICZ E M, FRANCZAK A. Periconceptional undernutrition affects the levels of DNA methylation in the peri-implantation pig endometrium and in embryos. Theriogenology, 2019,123:185-193.
[28]	WU H, ZHANG Y. Reversing DNA methylation: mechanisms, genomics, and biological functions. Cell, 2014,156(1-2):45-68.
[29]	BOMFIM M M, ANDRADE G M, DEL C M, SANGALLI J R, FONTES P K, NOGUEIRA M, MEIRELLES F V, DA S J, PERECIN F. Antioxidant responses and deregulation of epigenetic writers and erasers link oxidative stress and DNA methylation in bovine blastocysts. Molecular Reproduction and Development, 2017,84(12):1296-1305.
[30]	后晓南, 王智彪. 生命过程的相似性──从着床部位母体细胞的凋亡谈起. 生命科学, 2002,14:70-72.
	HOU X N, WANG Z B. Similarities in life processes--apoptosis in uterine epithelial cells at the time of embryo implantation. Chinese Bulletin of Life Sciences, 2002,14:70-72. (in Chinese)
[31]	FINK S L, COOKSON B T. Apoptosis, pyroptosis, and necrosis: mechanistic description of dead and dying eukaryotic cells. Infection and Immunity, 2005,73(4):1907-1916.
[32]	ZHAI Y, ZHANG Z, YU H, SU L, YAO G, MA X, LI Q, AN X, ZHANG S, LI Z. Dynamic methylation changes of DNA and H3K4 by RG108 improve epigenetic reprogramming of somatic cell nuclear transfer embryos in pigs. Cellular Physiology and Biochemistry, 2018,50(4):1376-1397.

基因名称 Gene name	引物序列（5'→3'） Primer sequence（5'→3'）	产物长度 Product size (bp)
β-actin	F：TGATATTGCTGCGCTCGTGGT R：GTCAGGATGCCTCTCTTGCTC	189
WNT5a	F：GCAAGGGCAATGTCTTCCA R：TTCATACCTAGCGACCACCAA	104
OPN	F: GTTAAACCGACCAGTTCTGGCA R: GTTGTCATCAGTTTCCTCAGAGG	148
NF-kB	F: GTGTAAAGAAGCGGGACTTGG R: GCGGTTGTCAAAGATGGGATG	203
Caspase9	F: CAATAGCAACCCTGAGACGGA R: AAAGCCTGGGAAGGTGGAGTA	130
GSDMD	F: TTATTGGCTCTGACTGGGACG R: GGCAAAGCTGAAGCACGAAC	128
BCL2	F: GAAACCCCGCCACGAATTA R: CCAGCCAACAGTACGGAACAA	96
BAX	F: TCTCCCCGAGAGGTCTTTTT R: TGATGGTCCTGATCAACTCG	151
NLRP3	F: CCGTCTGGGTGAGAGCGTGAA R: TCCTGTTGGCTCCTGTGTTCCT	78
Caspase1	F: GGATACAATAAATGGCTTGCTGG R: CTCGGGCTTTATCCATAGTTGT	110
TNF-α	F: CCACGTTGTAGCCAACATCAG R: AGATGAGGTAAAGCCCGTCAG	134
VEGFA	F: GTGACCCAGCACAGTTCCTCTT R: TTCCGGGCTCGGTGATTTAG	74
TET1	F: CATCACTGTCCGTCTTTGGA R: ACTTTCCTGTGCCTTTGTGG	126
TET2	F: GATACCATCTCCGTCTCCCATT R: CTTGCTGTCTCCATTCACTTCC	109
TET3	F: CCTTCTCCTTCGGTTGTTCCTG R: CACTTCTTCCTCTTTGGGGTTGTC	113

组成成分 Components	10 μL反应体系 10 μL reaction
Nuclease-Free Water	3.4 μL
cDNA	1 μL
Forward primer	0.3 μL
Reverse primer	0.3 μL
EvaGreen2x qPCR MasterMix	5 μL
Total	10 μL

		TET2	TET3	NFkB	TNFa	BAX	Bcl2	Caspase9	Caspase1	NLRP3	GSDMD
TET1	r	0.657	0.667	0.657	0.829*	-0.086	0.829*	0.371	0.314	0.257	0.429
	p	0.156	0.148	0.156	P＜0.05	0.872	P＜0.05	0.468	0.544	0.623	0.397
TET2			0.928**	1.000**	0.714	0.486	0.657	0.829*	0.714	0.771	0.771
			P＜0.01	P＜0.01	0.111	0.329	0.156	P＜0.05	0.111	0.072	0.072
TET3				0.928**	0.696	0.522	0.667	0.696	0.696	0.638	0.754
				P＜0.01	0.125	0.288	0.148	0.125	0.125	0.173	0.084
NF-kB					0.714	0.486	0.657	0.829*	0.714	0.771	0.771
					0.111	0.329	0.156	P＜0.05	0.111	0.072	0.072
TNFa						0.086	0.886*	0.543	0.657	0.6	0.6
						0.872	P＜0.05	0.266	0.156	0.208	0.208
Bax							0.314	0.771	0.771	0.714	0.829*
							0.544	0.072	0.072	0.111	P＜0.05
Bcl2								0.657	0.714	0.6	0.771
								0.156	0.111	0.208	0.072
Caspase9									0.886*	0.943**	0.943**
									P＜0.05	P＜0.01	P＜0.01
Caspase1										0.943**	0.943**
										P＜0.01	P＜0.01
NLRP3											0.886*
											P＜0.05

		TET2	TET3	NFkB	TNFa	BAX	Bcl2	Caspase9	Caspase1	NLRP3	GSDMD	OPN	WNT5a	VEGFA
TET1	r	-0.771	0.486	-0.257	0.086	0.029	0.143	-0.143	0.371	0.429	0.257	0.257	-0.429	-0.086
	p	0.072	0.329	0.623	0.872	0.957	0.787	0.787	0.468	0.397	0.623	0.623	0.397	0.872
TET2			-0.200	0.371	0.371	0.314	-0.086	0.600	-0.257	-0.429	0.029	-0.200	0.086	-0.029
			0.704	0.468	0.468	0.544	0.872	0.208	0.623	0.397	0.957	0.704	0.872	0.957
TET3				0.314	0.314	0.143	0.143	0.600	0.029	-0.086	0.086	0.200	-0.943^**	-0.886^*
				0.544	0.544	0.787	0.787	0.208	0.957	0.872	0.872	0.704	P＜0.01	P＜0.05
NFkB					0.714	0.600	0.600	0.714	-0.086	-0.029	0.086	-0.029	-0.543	-0.314
					0.111	0.208	0.208	0.111	0.872	0.957	0.872	0.957	0.266	0.544
TNFa						0.943^**	0.257	0.771	0.429	0.371	0.657	0.371	-0.486	-0.143
						P＜0.01	0.623	0.072	0.397	0.468	0.156	0.468	0.329	0.787
BAX							0.029	0.600	0.657	0.600	0.829^*	0.600	-0.257	-0.029
							0.957	0.208	0.156	0.208	P＜0.05	0.208	0.623	0.957
Bcl2								0.200	-0.486	-0.257	-0.429	-0.600	-0.429	0.086
								0.704	0.329	0.623	0.397	0.208	0.397	0.872
Caspase9									-0.029	-0.200	0.257	0.086	-0.714	-0.600
									0.957	0.704	0.623	0.872	0.111	0.208
Caspase1										0.943^**	0.943^**	0.943^**	0.086	0.086
										P＜0.01	P＜0.01	P＜0.01	0.872	0.872
NLRP3											0.829^*	0.829^*	0.143	0.257
											P＜0.01	P＜0.05	0.787	0.623
GSDMD												0.886^*	-0.029	0.029
												P＜0.05	0.957	0.957
OPN													-0.029	-0.200
													0.957	0.704
WNT5a														0.771
														0.072

TETs与细胞程序性死亡相关基因在山羊妊娠早期输卵管及子宫角的表达

Expression Patterns of TETs and Programmed Cell Death Related Genes in Oviduct and Uterus of Early Pregnancy Goats

RichHTML

PDF

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 32

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	张克坤,陈可钦,李婉平,乔浩蓉,张俊霞,刘凤之,房玉林,王海波. 灌水量对限根栽培‘阳光玫瑰’葡萄果实发育与香气物质积累的影响[J]. 中国农业科学, 2023, 56(1): 129-143.
[2]	古丽旦,刘洋,李方向,成卫宁. 小麦吸浆虫小热激蛋白基因Hsp21.9的克隆及在滞育过程与温度胁迫下的表达特性[J]. 中国农业科学, 2023, 56(1): 79-89.
[3]	束婧婷,单艳菊,姬改革,章明,屠云洁,刘一帆,巨晓军,盛中伟,唐燕飞,李华,邹剑敏. 广西麻鸡m6A甲基转移酶基因表达与肌纤维类型及成肌分化的关系[J]. 中国农业科学, 2022, 55(3): 589-601.
[4]	郭绍雷,许建兰,王晓俊,宿子文,张斌斌,马瑞娟,俞明亮. 桃XTH家族基因鉴定及其在桃果实贮藏过程中的表达特性[J]. 中国农业科学, 2022, 55(23): 4702-4716.
[5]	李恒,字向东,王会,熊燕,吕明杰,刘宇,蒋旭东. 基于全基因组重测序的山羊产羔数性状关键调控基因的筛选[J]. 中国农业科学, 2022, 55(23): 4753-4768.
[6]	郝艳,李晓颍,叶茂,刘亚婷,王天宇,王海静,张立彬,肖啸,武军凯. ‘21世纪’桃与‘久脆’桃及其杂交后代果实挥发性成分特征分析[J]. 中国农业科学, 2022, 55(22): 4487-4499.
[7]	康忱,赵雪芳,李亚栋,田哲娟,王鹏,吴志明. 黄瓜CC-NBS-LRR家族基因鉴定及在霜霉病和白粉病胁迫下的表达分析[J]. 中国农业科学, 2022, 55(19): 3751-3766.
[8]	温玉霞,张坚,王琴,王靖,裴悦宏,田绍锐,樊光进,马小舟,孙现超. 本氏烟NbMBF1c的克隆、表达及在TMV侵染过程中的功能[J]. 中国农业科学, 2022, 55(18): 3543-3555.
[9]	金梦娇,刘博,王抗抗,张广忠,钱万强,万方浩. 薇甘菊光能利用及叶绿素合成在不同光照强度下的响应[J]. 中国农业科学, 2022, 55(12): 2347-2359.
[10]	张卫东,郑玉杰,葛伟,张月朗,李芳,王昕. 单细胞测序对绒山羊毛乳头细胞的鉴定[J]. 中国农业科学, 2022, 55(12): 2436-2446.
[11]	杜宇,王永,孟庆勇,朱江江,林亚秋. 干扰山羊KLF12促进皮下脂肪细胞分化[J]. 中国农业科学, 2022, 55(1): 184-196.
[12]	袁景丽,郑红丽,梁先利,梅俊,余东亮,孙玉强,柯丽萍. 花青素代谢对陆地棉叶片和纤维色泽呈现的影响[J]. 中国农业科学, 2021, 54(9): 1846-1855.
[13]	束婧婷,姬改革,单艳菊,章明,巨晓军,刘一帆,屠云洁,盛中伟,唐燕飞,蒋华莲,邹剑敏. IGF1-PI3K-Akt信号通路相关基因在黄羽肉鸡肌肉和肝脏中的表达[J]. 中国农业科学, 2021, 54(9): 2027-2038.
[14]	赵珂,郑林,杜美霞,龙俊宏,何永睿,陈善春,邹修平. 柑橘SAR及其信号转导基因CsSABP2在黄龙病菌侵染中的响应特征[J]. 中国农业科学, 2021, 54(8): 1638-1652.
[15]	冯云奎,王健,马金亮,张柳明,李拥军. miR-31-5p对山羊毛囊干细胞增殖和凋亡的影响[J]. 中国农业科学, 2021, 54(23): 5132-5143.