中国农业科学 ›› 2020, Vol. 53 ›› Issue (19): 4057-4069.doi: 10.3864/j.issn.0578-1752.2020.19.018

• 食品科学与工程 • 上一篇    下一篇

非酿酒酵母在葡萄酒酿造中的应用

战吉宬(),曹梦竹,游义琳,黄卫东   

  1. 中国农业大学食品科学与营养工程学院/北京市葡萄科学与酿酒技术重点实验室,北京 100083
  • 收稿日期:2019-12-20 接受日期:2020-02-20 出版日期:2020-10-01 发布日期:2020-10-19
  • 通讯作者: 战吉宬
  • 基金资助:
    国家重点研发计划(2016YFD0400500)

Research Advance on the Application of Non-Saccharomyces in Winemaking

ZHAN JiCheng(),CAO MengZhu,YOU YiLin,HUANG WeiDong   

  1. College of Food Science and Nutritional Engineering, China Agricultural University/Beijing Key Laboratory of Viticulture and Enology, Beijing 100083
  • Received:2019-12-20 Accepted:2020-02-20 Online:2020-10-01 Published:2020-10-19
  • Contact: JiCheng ZHAN

摘要:

非酿酒酵母是葡萄酒微生物领域近些年的研究热点。过去人们对非酿酒酵母认识较少,曾将其看作是葡萄酒发酵的有害微生物。随着研究者们对酿酒微生物的深入研究发现,在酿酒过程中可以利用非酿酒酵母来改善葡萄酒的品质。合理利用非酿酒酵母可以酿造出色泽更加稳定、香气较丰富独特、口感更为复杂的葡萄酒。本文综述了近些年非酿酒酵母在葡萄酒酿造中的应用研究,包括生产中常见的非酿酒酵母种属的酿造特性、非酿酒酵母影响葡萄酒风味的机制、酵母之间的相互作用等3个方面。葡萄酒生产中常见的不同属(种)非酿酒酵母的酿造特性不同,其与酿酒酵母混合发酵对葡萄酒风味的改善效果也有较大差别。了解各个属(种)的发酵特性,根据发酵目的选择合适的接种菌株极为重要;非酿酒酵母可以通过不同的代谢通路改变葡萄酒中酒精、甘油、挥发性香气物质、甘露糖蛋白/多糖、花色苷等物质的含量,从而影响葡萄酒的颜色、香气和口感。非酿酒酵母代谢通路的特异性是其影响葡萄酒风味的根本原因。除了应用非酿酒酵母来改善葡萄酒的风味外,粟酒裂殖酵母降解苹果酸和耐热克鲁维酵母高产乳酸的特性也值得生产者关注。本文通过总结非酿酒酵母在葡萄酒酿造中应用的研究进展,以科学评价非酿酒酵母在葡萄酒酿造中的作用,并为建立新的具有科学性的混合发酵策略提供参考。

关键词: 非酿酒酵母, 酿酒酵母, 混合发酵, 风味, 葡萄酒

Abstract:

Non-Saccharomyces yeasts have become a hot research topic in wine microbiology recently. They were used to be considered as spoilage microbes in winemaking, but now many studies have found that non-Saccharomyces yeasts could improve wine quality by enhancing the color stability, the aroma and the taste. In this work, the recent studies on the application of non-Saccharomyces yeasts in winemaking were reviewed, including the fermentation characteristics of popular non-Saccharomyces yeasts, the role of non-Saccharomyces yeasts in modulation of wine flavor, and the interaction between yeasts during fermentation. Different non-Saccharomyces yeasts have distinctive fermentation characteristics and thus affect the wine flavor differently when they are co-inoculated with Saccharomyces cerevisiae. Selection of appropriate non-Saccharomyces yeasts based on their fermentation characteristics is critical to produce the desired wine style. Non-Saccharomyces yeasts can affect the concentrations of alcohol, glycerol, volatile aroma compounds, mannoproteins/polysaccharides, anthocyanins and other substances in wine through various metabolic pathways, which is quite different from Saccharomyces cerevisiae. In addition to the wine flavor enhancement by non-Saccharomyces yeasts, the malolactic fermentation by Schizosaccharomyces pombe and the high efficiency lactic acid production by Kluyveromyces thermotolerans should also be paid attention for their applications in wine production. This paper summarized the research progress in the application of non-Saccharomyces yeasts in winemaking, in order to evaluate the role of non-Saccharomyces in wine production and provide valuable information on inoculation strategy for co-fermentation.

Key words: non-Saccharomyces, Saccharomyces cerevisiae, co-fermentation, flavor, wine