中国农业科学 ›› 2021, Vol. 54 ›› Issue (19): 4196-4206.doi: 10.3864/j.issn.0578-1752.2021.19.014

• 园艺 • 上一篇    下一篇

甜瓜SLAF图谱构建及果实相关性状QTL分析

王岭1(),才羿1,王桂超1,王迪2,盛云燕1()   

  1. 1黑龙江八一农垦大学园艺园林学院,黑龙江大庆 163319
    2黑龙江省农业科学院大庆分院,黑龙江大庆 163319
  • 收稿日期:2020-11-25 接受日期:2021-03-16 出版日期:2021-10-01 发布日期:2021-10-12
  • 通讯作者: 盛云燕
  • 作者简介:王岭,E-mail: wlbynd@163.com
  • 基金资助:
    国家自然科学基金(31772330);中央引导地方科技发展专项(ZY19C02)

Specific Length Amplified Fragment (SFLA) Sequencing Mapping Construction and QTL Analysis of Fruit Related Traits in Muskmelon

WANG Ling1(),CAI Yi1,WANG GuiChao1,WANG Di2,SHENG YunYan1()   

  1. 1College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang
    2Daqing Branch of Heilongjiang Academy of Agricultural Sciences, Daqing 163319, Heilongjiang
  • Received:2020-11-25 Accepted:2021-03-16 Online:2021-10-01 Published:2021-10-12
  • Contact: YunYan SHENG

摘要:

【目的】通过对甜瓜果实相关性状进行QTL定位及候选基因分析,为甜瓜品质育种及基因挖掘与功能验证提供理论基础。【方法】利用薄皮甜瓜1244为母本、厚皮甜瓜M5为父本配置杂交组合,结合SFLA测序技术开发分子标签,构建高密度遗传图谱,以F2:3表型数据为基础,采用Mapqtl进行QTL定位分析。【结果】获得了380 446 Mreads(83.12 Gb)数据,测序平均Q30为93.59%,平均GC含量为36.87%;开发了112 844个SLAF标签,3 274 879个SNP;构建了12个连锁群,共计10 596个上图标记,总图距为1 383.88 cM,标记间平均距离为0.13 cM,上图标记完整度平均为99.92%。将控制甜瓜果面沟性状基因(fst)定位在第11条染色体末端Marker 1993423(62.18)—Marker 1998820(63.05),覆盖基因组0.72 Mb,包含33个候选基因;控制甜瓜果皮花纹基因(fpp)定位在第2条染色体Marker 459584(90.91)—Marker 459446(90.91),覆盖基因组0.08 Mb,包含5个候选基因,其中MELO3C026292(1-脱氧-D-木酮糖-5-磷酸还原异构酶)可能为控制果皮花纹的候选基因;同时检测到甜瓜果皮底色1个QTL位点fpc,位于第7条染色体Marker 1229174(7.14)—Marker 1229973(7.14),贡献率为9.9%;检测到果型指数1个QTL位点fs9.1,位于第9条染色体Marker 1705671(76.19)—Marker 1705915(79.23),贡献率为7.6%;在第1、2、6、7、10染色体检测到单果重相关6个QTL位点(sfw1.1sfw2.1sfw2.2sfw6.1sfw7.1sfw10.1),贡献率在3.1%—17.0%,LOD值介于3.0—5.6。【结论】将甜瓜果面沟、果皮花纹基因分别定位在第11和第2条染色体,分别获得33个和5个候选基因;检测到1个果皮底色QTL位点、1个果型指数QTL位点和6个单果重QTL位点。

关键词: 甜瓜, SLAF测序, 遗传图谱构建, 果实性状, QTL分析

Abstract:

【Objective】 Through the QTL mapping and candidate gene analysis of muskmelon (Cucumis melo L.) fruit-related traits, so as to provide a theoretical basis for muskmelon quality breeding, gene mapping and functional verification. 【Method】 The F1 plant was made cross by 1244 as female parent with thin-skinned melon and M5 with thick-skinned melon as male parent, combined with SFLA sequencing technology to develop molecular tags and construct high-density genetic maps. Based on F2:3 phenotype data, Mapqtl analysis method was used to detect QTL location. 【Result】 Totally 380 446 Mreads (83.12 Gb) data were obtained, the average Q30 and GC content was 93.59% and 36.87%, respectively; totally 112 844 SLAF tags and 3 274 879 SNPs were obtained; a genetic map contained 12 linkage groups with a total of 10 596 markers were constructed. The genetic map spanned 1 383.88 cM with the average distance was 0.13 cM between markers, and totally 99.92% developed markers were mapped. The gene controling fruit sutures (fst) of melon was located at the end of chromosome 11, between Marker 1993423 (62.18) and Marker 1998820 (63.05), covering 0.72 Mb of the genome, and containing 33 candidate genes. The gene for pericarp pattern (fpp) was located at chromosome 2 between Marker 459584 (90.91) and Marker 459446 (90.91) which covered the genome of 0.08 Mb and contained 5 candidate genes, among them MELO3C026292 (1-deoxy-D-xylulose-5-phosphate reductoisomerase) might be the candidate gene for pericarp pattern. At the same time, QTL locus of pericarp color (fpc) was detected which located between Marker 1229174 (7.14) and Marker 1229973 (7.14) on the chromosome 7, with the contribution rate was 9.9%. One QTL locus of fruit shape (fs) fs9.1 was detected between Marker 1705671 (76.19) and Marker 1705915 (79.23) on chromosome 9 with a contribution rate of 7.6%. Six QTL loci related to single fruit weight (sfw1.1, sfw2.1, sfw2.2, sfw6.1, sfw7.1, sfw10.1) were detected on chromosomes 1, 2, 6, 7, and 10 with the contribution rate were between 3.1% to 17%, and the LOD value were between 3.0 to 5.6. 【Conclusion】The fruit sutures and pericarp pattern genes were located on the chromosomes11 and 2, and then 33 and 5 candidate genes were obtained, respectively; One QTL for pericarp color, one QTL for fruit shape and 6 QTLs for single fruit weight were also detected.

Key words: melon, SLAF sequencing, genetic map construction, fruit related traits, QTL analysis