植物青枯菌遗传多样性及致病基因组学研究进展

doi:10.3864/j.issn.0578-1752.2013.14.006

摘要/Abstract

摘要： 植物细菌性青枯病是由茄科雷尔氏菌（Ralstonia solanacearum Yabuuchi et al.）引起的一种世界性重大病害。作为复合种，青枯菌在与寄主长期协同进化过程中，表现出广泛的生态和寄主适应性。Fengan和Prior提出青枯菌演化型分类框架，用以描述青枯菌种以下的遗传多样性。青枯菌种内表型特征差异的本质是其基因组较其它植物病原细菌更为复杂和更具可塑性。笔者对近期青枯菌遗传多样性和致病基因组学方面的研究进展进行了归纳和讨论。

关键词: 青枯菌 , 遗传多样性 , 演化型 , 致病基因组学

Abstract: Bacterial wilt, caused by Ralstonia solanacearum, is one of the most devastating plant diseases worldwide. As a species complex, R. solanacearum strains are highly variable and adaptable as attested by worldwide distribution and a wide and expanding host range. A new phylotype classification scheme was proposed by Fegan and Prior to distinguish the genetic diversity within the R. solanacearum species complex. High genome complexity and plasticity of R. solanacearum is reflected by its great adaptation potential to different hosts and environments. This review includes recent advances in research of genetic diversity and pathogenome of R. solanacearm species complex.

Key words: Ralstonia solanacearum , genetic diversity , phylotype classification scheme , pathogenome

徐进, 冯洁. 植物青枯菌遗传多样性及致病基因组学研究进展[J]. 中国农业科学, 2013, 46(14): 2902-2909.

XU Jin, FENG Jie. Advances in Research of Genetic Diversity and Pathogenome of Ralstonia solanacearum Species Complex[J]. Scientia Agricultura Sinica, 2013, 46(14): 2902-2909.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2013.14.006

https://www.chinaagrisci.com/CN/Y2013/V46/I14/2902

参考文献

[1]Wicker E, Grassart L, Coranson-Beaudu R, Mian D, Guilbaud C, Fegan M, Prior P. Ralstonia solanacearum strains from Martinique (French West Indies) exhibiting a new pathogenic potential. Applied and Environmental Microbiology, 2007, 71(21): 6790-6801.

[2]Xu J, Pan Z C, Prior P. Xu J S, Zhang Z, Zhang H, Zhang L Q, He L Y, Feng J. Genetic diversity of Ralstonia solanacearum strains from China. European Journal of Plant Pathology, 2009, 125(4): 641-653.

[3]潘哲超, 徐进, 顾钢, 吴畏, 许景升, 陈顺辉, 冯洁. 福建及贵州等地烟草青枯菌系统发育分析. 植物保护, 2012, 38(1): 18-23.

Pan Z C, Xu J, Gu G, Wu W, Wu W, Xu J S, Chen S H, Feng J. Phylogeny of tobacco Ralstonia solanacearum strains from Fujian and Guizhou Provinces. Plant Protection, 2012, 38(1): 18-23. (in Chinese)

[4]Buddenhagen I, Sequeira L, Kelman A. Designation of races in Pseudomonas solanacearum. Phytopathology, 1962, 52(2): 726.

[5]Hayward A C. Characteristics of Pseudomonas solanacearum. Journal of Applied Microbiology, 1964, 27(2): 265-277.

[6]Hayward A C. Systematics and phylogeny of Pseudomonas solanacearum and related bacteria//Hayward A C, Hartman G L. Bacterial Wilt: The Disease and Its Causative Agent, Pseudomonas solanacearum. United Kingdom: CAB International, 1994: 123-135.

[7]He L Y, Sequeira L, Kelman A. Characteristics of strains of Pseudomonas solanacearum from China. Plant Disease, 1983, 67(12): 1357-1361.

[8]Gillings M R, Fahy P. Genomic fingerprinting: towards a unified view of the Pseudomonas solanacearum species complex//Hayward A C, Hartman G L. Bacterial Wilt: The Disease and Its Causative Agent, Pseudomonas solanacearum. Wallingford: CAB International, 1994: 95-112.

[9]Cook D, Barlow E, Sequeira L. Genetic diversity of Pseudomonas solanacearum: detection of restriction fragment polymorphisms with DNA probes that specify virulence and hypersensitive response. Molecular Plant-Microbe Interactions, 1989, 2(3): 113-121.

[10]Cook D, Sequeira L. Strain differentiation of Pseudomonas solanacearum by molecular genetic methods//Hayward A C, Hartman G L. Bacterial Wilt: The disease and Its Causative Agent, Pseudomonas solanacearum. United Kingdom: CAB International, 1994: 77-93.

[11]Fegan M, Taghavi M, Sly L I, Hayward A C. Phylogeny, diversity and molecular diagnostics of Ralstonia solanacearum//Prior P, Allen C, Elphinstone J. Bacterial Wilt Disease: Molecular and Ecological Aspects. Paris, France: INRA Editions, 1998: 19-33.

[12]Poussier S, Vandewalle P, Luisetti J. Genetic diversity of African and worldwide strains of Ralstonia solanacearum as determined by PCR-restriction fragment length polymorphism analysis of the hrp gene region. Applied and Environmental Microbiology, 1999, 65(5): 2184-2194.

[13]Prior P, Fegan M. Recent development in the phylogeny and classification of Ralstonia solanacearum. Acta Horticulturae, 2005, 695(2): 127-136.

[14]Prior P, Fegan M. Diversity and molecular detection of Ralstonia solanacearum race 2 strains by multiplex PCR//Allen C, Prior P, Hayward A C. Bacterial Wilt Disease and The Ralstonia solanacearum Species Complex. St. Paul, USA: APS Press, 2005: 405-414.

[15]Hayward A C. Biology and epidemiology of bacterial wilt caused by Pseudomonas solanacearum. Annual Review of Phytopathology, 1991, 29: 67-87.

[16]Xu J, Zheng H J, Liu L, Pan Z C, Prior P, Tang B, Xu J S, Zhang H, Tian Q, Zhang L Q, Feng J. Complete genome sequence of the plant pathogen Ralstonia solanacearum strain Po82. Journal of Bacteriology, 2011, 193(16): 4261-4262.

[17]Salanoubat M, Genin S, Artiguenave F, Gouzy J, Mangenot S, Arlat M, Billault A, Brottier P, Camus J C, Cattolico L, Chandler M, Choisne N, Claudel-Renard C, Cunnac S, Demange N, Gaspin C, Lavie M, Moisan A, Robert C, Saurin W, Schiex T, Siguier P, Thébault P, Whalen M, Wincker P, Levy M, Weissenbach J, Boucher C A. Genome sequence of the plant pathogen Ralstonia solanacearum. Nature, 2002, 415: 497-502.

[18]Remenant B, Coupat-Goutaland B, Guidot A, Cellier G, Wicker E, Allen C, Fegan M, Pruvost O, Elbaz M, Calteau A, Salvignol G, Mornico D, Mangenot S, Barbe V, Médigue C, Prior P. Genomes of three tomato pathogens within the Ralstonia solanacearum species complex reveal significant evolutionary divergence. BMC Genomics, 2010, 11: 379.

[19]Remenant B, de Cambiaire J C, Cellier G, Jacobs J M, Mangenot S, Barbe V, Lajus A, Vallent D, Medigue C, Fegan M, Allen C, Prior P. Ralstonia syzygii, the blood disease bacterium and some Asian R. solanacearum strains form a single genomic species despite divergent lifestyles. PLoS ONE, 2011, 6: e24356.

[20]Guidot A, Elbaz M, Carrère S, Siri M I, Pianzzola M J, Prior P, Boucher C. Specific genes from the potato brown rot strains of Ralstonia solanacearum and their potential use for strain detection. Phytopathology, 2009, 99(9): 1105-1112.

[21]Li Z, Wu S, Bai X, Liu Y, Lu J, Liu Y, Xiao B, Lu X, Fan L. Genome sequence of the tobacco bacterial wilt pathogen Ralstonia solanacearum. Journal of Bacteriology, 2011, 193(21): 6088-6089.

[22]Gabriel D W, Allen C, Schell M, Denny T P, Greenberg J T, Duan Y P, Flores-Cruz Z, Huang Q, Clifford J M, Presting G, González E T, Reddy J, Elphinstone J, Swanson J, Yao J, Mulholland V, Liu L, Farmerie W, Patnaikuni M, Balogh B, Norman D, Alvarez A, Castillo J A, Jones J, Saddler G, Walunas T, Zhukov A, Mikhailova N. Identification of open reading frames unique to a select agent: Ralstonia solanacearum race 3 biovar 2. Molecular Plant-Microbe Interactions, 2006, 19(1): 69-79.

[23]Guidot A, Prior P, Schoenfeld J, Carrere S, Genin S, Boucher C. Genomic structure and phylogeny of the plant pathogen Ralstonia solanacearum inferred from gene distribution analysis. Journal of Bacteriology, 2007, 189(2): 377-387.

[24]Medini D, Donati C, Tettelin H, Masignani V, Rappuoli R. The microbial pan-genome. Current Opinion in Genetics & Development, 2005, 15(6): 589-594.

[25]Bertolla F, Frostegard A, Brito B, Nesme X, Simonet P. During infection of its host, the plant pathogen Ralstonia solanacearum naturally develops a state of competence and exchanges genetic material. Molecular Plant-Microbe Interactions, 1999, 12(5): 467-472.

[26]Guidot A, Coupat B, Fall S, Prior P, Bertolla F. Horizontal gene transfer between Ralstonia solanacearum strains detected by comparative genomic hybridization on microarrays. The ISME Journal, 2009, 3: 549-562.

[27]Nakamura Y, Itoh T, Matsuda H, Gojobori T. Biased biological functions of horizontally transferred genes in prokaryotic genomes. Nature Genetics, 2004, 36: 760-766.

[28]Coupat-Goutaland B, Bernillon D, Guidot A, Prior P, Nesme X, Bertolla1 F. Ralstonia solanacearum virulence increased following large interstrain gene transfers by natural transformation. Molecular Plant-Microbe Interactions, 2011, 24(4): 497-505.

[29]Deslandes L, Olivier J, Peeters N, Feng D X, Khounlotham M, Boucher C, Somssich I, Genin S, Marco Y. Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus. Proceedings of the National Academy of Sciences of the United States of America, 2003, 100(13): 8024-8029.

[30]Lavie M, Shillington E, Eguiluz C, Grimsley N, Boucher C. PopP1, a new member of the YopJ/AvrRxv family of type III effector proteins, acts as a host-specificity factor and modulates aggressiveness of Ralstonia solanacearum. Molecular Plant-Microbe Interactions, 2002, 15(10): 1058-1068.

[31]潘哲超. 植物青枯菌遗传多样性及致病力分化研究[D]. 北京: 中国农业科学院, 2010.

Pan Z C. Genetic diversity and pathogenicity variation of Ralstonia solanacearum[D]. Beijing: Chinese Academy of Agricultural Sciences, 2010. (in Chinese)

[32]Schell M A. Control of virulence and pathogenicity genes of Ralstonia solanacearum by an elaborate sensory network. Annual Review of Phytopathology, 2000, 38: 263-292.

[33]Hikichi Y, Yoshimochi T, Tsujimoto S, Shinohara R, Nakaho K, Kanda A, Kiba A, Ohnishi K. Global regulation of pathogenicity mechanism of Ralstonia solanacearum. Plant Biotechnology, 2007, 24(1): 149-154.

[34]Mole B M, Baltrus D A, Dangl J L, Grant S R. Global virulence regulation networks in phytopathogenic bacteria. TRENDS in Microbiology, 2007, 15(8): 363-371.

[35]Genin S, Denny T P. Pathogenomics of the Ralstonia solanacearum species complex. Annual Review of Phytopathology, 2012, 50: 67-89.

[36]Brumbley S M, Carney B F, Denny T P. Phenotype conversion in Pseudomonas solanacearum due to spontaneous inactivation of PhcA, a putative LysR transcriptional regulator. Journal of Bacteriology, 1993, 175(17): 5477-5487.

[37]Cunnac S, Occhialini A, Barberis P, Boucher C, Genin S. Inventory and functional analysis of the large Hrp regulon in Ralstonia solanacearum: identification of novel effector proteins translocated to plant host cells through the type III secretion system. Molecular Microbiology, 2004, 53(1): 115-128.

[38]Cunnac S, Boucher C, Genin S. Characterization of the cis-acting regulatory element controlling HrpB-mediated activation of the type III secretion system and effector genes in Ralstonia solanacearum. Journal of Bacteriology, 2004, 186(8): 2309-2318.

[39]Occhialini A, Cunnac S, Reymond N, Genin S, Boucher C. Genome-wide analysis of gene expression in Ralstonia solanacearum reveals that the hrpB gene acts as a regulatory switch controlling multiple virulence pathways. Molecular Plant-Microbe Interactions, 2005, 18(9): 938-949.

[40]Carney B F, Denny T P. A cloned avirulence gene from Pseudomonas solanacearum determines incompatibility on Nicotiana tabacum at the host species level. Journal of Bacteriology, 1990, 172(9): 4836-4843.

[41]Jeong E L, Timmis J N. Novel insertion sequence elements associated with genetic heterogeneity and phenotype conversion in Ralstonia solanacearum. Jounal of Bacteriology, 2000, 182(16): 4673-4676.

[42]Lavie M, Seunes B, Prior P, Boucher C. Distribution and sequence analysis of a family of type III-dependent effectors correlate with the phylogeny of Ralstonia solanacearum strains. Molecular Plant-Microbe Interactions, 2004, 17(8): 931-940.

[43]Poueymiro M, Cunnac S, Barberis P, Deslandes L, Peeters N, Cazale-Noel A C, Boucher C, Genin S. Two type III secretion system effectors from Ralstonia solanacearum GMI1000 determine host range specificity on tobacco. Molecular Plant-Microbe Interactions, 2009, 22(5): 538-550.

[44]Robertson A E, Wechter W P, Denny T P, Fortnum B A, Kluepfel D A. Relationship between avirulence gene (avrA) diversity in Ralstonia solanacearum and bacterial wilt incidence. Molecular Plant-Microbe Interactions, 2004, 17(12): 1376-1384.

[45]Angot A, Peeters N, Lechner E, Vailleau F, Baud C, Gentzbittel L, Sartorel E, Genschik P, Boucher C, Genin S. Ralstonia solanacearum requires F-box-like domain-containing type III effectors to promote disease on several host plants. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103(39): 14620-14625.

[46]Angot A, Vergunst A, Genin S, Peeters N. Exploitation of eukaryotic ubiquitin signaling pathways by effectors translocated by bacterial type III and type IV secretion systems. PLoS Pathogens, 2007, 3: e3.

[47]Solé M, Popa C, Mith O, Sohn K H, Jones J D G, Deslandes L, Valls M. The awr gene family encodes a novel class of Ralstonia solanacearum type III effectors displaying virulence and avirulence activities. Molecular Plant-Microbe Interactions, 2012, 25(7): 941-953.

[48]Remigi P, Anisimova M, Guidot A, Genin S, Peeters N. Functional diversification of the GALA type III effector family contributes to Ralstonia solanacearum adaptation on different plant hosts. New Phytologist, 2011, 192(4): 976-987.

[49]Konstantinidis K T, Tiedje J M. Genomic insights that advance the species definition for prokaryotes. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(7): 2567-2572.

[50]Wicker E, Lefeuvre P, Cambiaire J C, Lemaire C, Poussier S, Prior P. Contrasting recombination patterns and demographic histories of the plant pathogen Ralstonia solanacearum inferred from MLSA. The ISME Journal, 2012, 6: 961-974.

[51]Jacobs J M, Babujee L, Meng F, Milling A, Allen C. The in planta transcriptome of Ralstonia solanacearum: conserved physiological and virulence strategies during bacterial wilt of tomato. mBio, 3(4): e00114-12.