甘蓝型油菜开花调控转录因子CONSTANS的表达分析

doi:10.3864/j.issn.0578-1752.2013.12.021

中国农业科学 ›› 2013, Vol. 46 ›› Issue (12): 2592-2598.doi: 10.3864/j.issn.0578-1752.2013.12.021

甘蓝型油菜开花调控转录因子CONSTANS的表达分析

郑本川, 张锦芳, 李浩杰, 柴靓, 崔成, 蒋俊, 蒲晓斌, 牛应泽, 蒋梁材

1.四川省农业科学院作物研究所，成都 610066
2.四川农业大学农学院，四川温江 611130

收稿日期:2013-01-04 出版日期:2013-06-15 发布日期:2013-04-01
通讯作者: 通信作者蒋梁材，Tel：13708191632；E-mail：jlcrape@163.com。通信作者牛应泽，Tel：18981729841；E-mail：nyz@sicau.edu.cn
作者简介:郑本川，Tel：13540891443；E-mail：zhengbenchuan1987@163.com
基金资助:
国家现代农业产业技术体系建设专项资金、国家“863”计划（2011AA10A104）、国家科技支撑计划（2011BAD35B04）、四川省育种攻关（2011NZ0098-5， 2011NZ0098-16）、四川省财政基因工程（2011JYGC04-013）、四川省农业创新团队建设专项资金、四川省杰出青年学术技术带头人培育计划（2010JQ0054）

Analysis of Quantitative Expression of the Flowering-Regulating Transcription Factor CONSTANS Gene in Brassica napus L.

ZHENG Ben-Chuan, ZHANG Jin-Fang, LI Hao-Jie, CHAI Liang, CUI Cheng, JIANG Jun, PU Xiao-Bin, NIU Ying-Ze, JIANG Liang-Cai

1.Crops Research Institute, Sichuan Academy of Agricultural Science, Chengdu 610066
2.College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, Sichuan

Received:2013-01-04 Online:2013-06-15 Published:2013-04-01

摘要/Abstract

摘要： 【目的】研究开花调控转录因子CONSTANS（CO）同源基因在甘蓝型油菜中的表达特征。【方法】以早熟甘蓝型油菜品系D626-6和晚熟甘蓝型油菜品系D125-5为材料，依据甘蓝型油菜CONSTANS同源基因Bn1CON19设计特异性引物扩增CO基因全长编码区，并根据获得的cDNA序列设计实时荧光定量特异性引物，采用SYBR Green I染料法进行实时荧光定量PCR研究CO基因表达差异。【结果】在整个生育期内，早熟和晚熟甘蓝型油菜品系的CO基因以叶片中的表达量最高，花蕾和茎中表达量次之，且早晚表达量高于中午时分；在不同生育时期内，抽薹期表达量最大，且早熟甘蓝型油菜品系CO基因在叶片和花蕾中的表达明显高于晚熟甘蓝型油菜品系。【结论】CO同源基因在甘蓝型油菜成花过程中以及生育期的长短上发挥着一定的作用。

关键词: 甘蓝型油菜 , 转录调控因子 , CO基因（CONSTANS） , 实时荧光定量PCR

Abstract: 【Objective】The expression of CONSTANS(CO) homologous genes in B. napus was characterized. 【Method】Materials D626-6 and D125-5 with different growth periods were used to characterize the expression level of CO gene. A pair of specific primers was designed based on Bn1CON19 cDNA sequence, and a pair of Real-time fluorescence quantitative specific primers was designed according to the obtained cDNA sequence. The Real-time fluorescence quantitative analysis was conducted by using SYBR Green I dye method.【Result】 The results showed that the highest expression levels were appeared in the leaves of B. napus L., followed by the buds and stems. The expression levels in the morning and the evening were higher than those at noon. At different growth stages, the highest expression levels were appeared at bolting stage. The early-maturing material had a significantly higher express level than the late-maturing material in leaves and buds at bolting stage. 【Conclusion】 Result of the study indicated that the CO gene might play an important role in growth period and flower formation process in B. napus L..

Key words: Brassica napus L. , transcription factor , CO gene (CONSTANS) , qRT-PCR

郑本川, 张锦芳, 李浩杰, 柴靓, 崔成, 蒋俊, 蒲晓斌, 牛应泽, 蒋梁材. 甘蓝型油菜开花调控转录因子CONSTANS的表达分析[J]. 中国农业科学, 2013, 46(12): 2592-2598.

ZHENG Ben-Chuan, ZHANG Jin-Fang, LI Hao-Jie, CHAI Liang, CUI Cheng, JIANG Jun, PU Xiao-Bin, NIU Ying-Ze, JIANG Liang-Cai. Analysis of Quantitative Expression of the Flowering-Regulating Transcription Factor CONSTANS Gene in Brassica napus L.[J]. Scientia Agricultura Sinica, 2013, 46(12): 2592-2598.

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甘蓝型油菜开花调控转录因子CONSTANS的表达分析

Analysis of Quantitative Expression of the Flowering-Regulating Transcription Factor CONSTANS Gene in Brassica napus L.

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