小麦灌浆期耐热性QTL定位分析

doi:10.3864/j.issn.0578-1752.2013.10.018

摘要/Abstract

摘要： 【目的】以普通小麦加倍单倍体（DH）群体（旱选10号×鲁麦14）的150个株系为材料，鉴定其灌浆期耐热相关生理性状及千粒重耐热指数，并进行QTL定位，以期发掘具有显著效应以及不同环境中稳定表达的主效QTL，为改良小麦耐热性提供理论依据及分子标记。【方法】运用基于混合线性模型的复合区间作图法，以耐热指数为耐热性指标，对DH群体在田间雨养和灌溉2种土壤水分条件下的耐热性进行QTL定位。【结果】2种土壤水分条件下共检测到12个控制不同性状耐热指数的加性效应QTL，对表型变异的贡献率范围为2.64%—11.41%，其中，9个QTL与环境存在互作效应，对耐热指数表型变异的贡献率为1.41%—4.66%；检测到17对上位性效应QTL，对表型变异的贡献率为2.45%—8.84%，其中，仅4对与环境有互作效应，对表型变异的贡献率为0.62%—2.32%。控制耐热性的QTL来自双亲，DH群体中有耐热性超亲的株系存在。【结论】评价小麦灌浆期的耐热性，千粒重耐热指数是最直接的指标，生理性状指标为耐热性鉴定的间接辅助指标，其中，旱地条件下选用旗叶相对含水量耐热指数作为间接指标较好，而灌溉条件下选用气冠温差耐热指数较好。染色体1B、2D、5A、5B、6A、6B和7A对灌浆期耐热性贡献较大。千粒重耐热指数和旗叶叶绿素含量耐热指数的遗传以加性效应为主，叶绿素荧光参数耐热指数和气冠温差耐热指数的遗传以上位性效应为主，而叶片相对含水量耐热指数的遗传加性效应与上位性效应都重要。

关键词: 小麦 , 耐热指数 , 加性效应 , 上位性效应 , 基因与环境互作

Abstract: 【Objective】In this study, a doubled haploid (DH) population with 150 lines, which was derived from the cross of two Chinese common wheat cultivars Hanxuan 10 and Lumai 14, was used as the plant material to identify the heat tolerance index (HTI) for related physiological traits and thousand-grain weight (TGW) at grain filling stage in common wheat and carry out quantitative trait loci (QTL) analysis. The purpose was to identify the essential QTL with stable and remarkable effects and find a theoretical basis on marker-assisted selection for improving the heat tolerance in wheat breeding program. 【Method】 The mixed-model-based composite interval mapping method was employed to identify QTL for HTI of related traits in two soil moisture environments. 【Result】A total of 12 additive QTL and 17 epistatic QTL for HTI of TGW and physiological traits related to heat tolerance were located on all chromosomes except 1D, 6D and 7B under two soil moisture conditions. One single additive QTL can explain 2.64%-11.41% phenotypic variance for HTI, and a pair of epistatic QTL can explain 2.45%-8.84% phenotypic variance for HTI. Nine of 12 additive QTL have interaction effects with the environments, and the interaction effect of single additive QTL with the environment can explain 1.41%-4.66% phenotypic variance for HTI. Only 5 of the 17 epistatic QTL exist interaction effects with the environments, and the interaction effect of single pair of QTL with the environment can explain 0.62%-2.32% phenotypic variance for HTI. The allelic contribution to the HTI QTL came from both parents. Some DH lines were more tolerant to the heat stress than their parents.【Conclusion】 To evaluate the heat tolerance at grain filling stage, the HTI for TGW is a direct criteria, the following two physiological index are indirect criteria, i.e. the HTI for flag leaves relative water content (IRWC) is an available index under rainfed condition, and the HTI for canopy temperature depression (ICTD) is suitable under well-watered condition. The QTL for HTIs mainly distribute on the chromosomes 1B, 2D, 5A, 5B, 6A, 6B and 7A, showing that these chromosomes have close relationship with heat-tolerance at grain filling stage. The genetic effects of QTL for TGW HTI and chlorophyll content (CC) mainly are additive effect, and that of CTD and chlorophyll fluorescence parameters (CFP) are mainly epistatic effect. The additive effect and the epistatic effect are almost equal in the genetic effect of the QTL for the RWC HTI.

Key words: wheat , heat tolerance index (HTI) , additive effect , epistatic effect , G×E interactions

李世平, 昌小平, 王成社, 景蕊莲. 小麦灌浆期耐热性QTL定位分析[J]. 中国农业科学, 2013, 46(10): 2119-2129.

LI Shi-Ping, CHANG Xiao-Ping, WANG Cheng-She, JING Rui-Lian. Mapping QTL for Heat Tolerance at Grain Filling Stage in Common Wheat[J]. Scientia Agricultura Sinica, 2013, 46(10): 2119-2129.

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参考文献

[1]Ortiz R, Sayre K D, Govaerts B, Gupta R, Subbarao G V, Ban T, Hodson D, Dixon J M, Ortiz-Monasterio J I, Reynolds M. Climate change: Can wheat beat the heat? Agriculture, Ecosystems and Environment, 2008, 126: 46-58.

[2]Fischer R A. Wheat physiology: A review of recent developments. Crop and Pasture Science, 2011, 62: 95-114.

[3]Matasuki J, Yasui T, Kohyama K, Sasaki T. Effects of environmental temperature on structure and gelatinizati on properties of wheat starch. Cereal Chemistry, 2003, 80: 476-480.

[4]Asseng S, Fosterw I, Turnerz N. The impact of temperature variability on wheat yields. Global Change Biology, 2011, 17: 997-1012.

[5]金善宝. 中国小麦学. 北京: 中国农业出版社, 1996: 97.

Jin S B. Chinese Wheat. Beijing: Chinese Agricultural Press, 1996: 97. (in Chinese)

[6]李永庚, 于振文, 张秀杰, 高雷明. 小麦产量与品质对灌浆不同阶段高温胁迫的响应. 植物生态学报, 2005, 29(3): 461-466.

Li Y G, Yu Z W, Zhang X J, Gao L M. Response of yield and quality of wheat to heat stress at different grain filling stages. Acta Phytoecologica Sinica, 2005, 29(3): 461-466. (in Chinese)

[7]Wahid A, Gelania S, Ashrafa M, Foolad M R. Heat tolerance in plants: An overview. Journal of Environmental and Experimental Botany, 2007, 61: 199-223.

[8]气候变化对农业的影响及其对策课题组. 气候变化对农业的影响及其对策. 北京: 北京大学出版社, 1993.

Research group for the impact and countermeasures of climate change on agriculture. The Impact and Countermeasures of Climate Change on Agriculture. Beijing: Peking University Press, 1993. (in Chinese)

[9]Vijayalakshmi K, Fritz A K, Paulsen G M, Bai G, Pandravada S, Gill B S. Modeling and mapping QTL for senescence-related traits in winter wheat under high temperature. Molecular Breeding, 2010, 26: 163-175.

[10]Mason R E, Mondal S, Beecher F W, Pacheco A, Jampala B, Ibrahim A M H, Hays D B. QTL associated with heat susceptibility index in wheat (Triticum aestivum L.) under short-term reproductive stage heat stress. Euphytica, 2010, 174: 423-436.

[11]陈希勇, 赵爱菊, 李亚军. 小麦耐热性基因的染色体定位和遗传效应分析. 华北农学报, 2007, 22(增刊): 1-5.

Chen X Y, Zhao A J, Li Y J. Wheat heat resistance gene chromosome mapping and genetic effect analysis. Acta Agriculturae Boreali-Sinica, 2007, 22(supplement): 1-5. (in Chinese)

[12]马晓娣, 彭慧茹, 汪矛, 王丽, 孙其信. 作物耐热性的评价. 植物学通报, 2004, 21(4): 411-418.

Ma X D, Peng H R, Wang M, Wang L, Sun Q X. Evaluation of heat tolerance in crop. Chinese Bulletin of Botany, 2004, 21(4): 411-418. (in Chinese)

[13]景蕊莲, 昌小平, 贾继增, 胡荣海. 用花药培养创建小麦加倍单倍体作图群体. 生物技术, 1999, 9(3): 4-8.

Jing R L, Chang X P, Jia J Z, Hu R H. Establishing wheat doubled haploid population for genetic mapping by anther culture. Biotechnology, 1999, 9(3): 4-8. (in Chinese)

[14]Li S P, Chang X P, Wang C S, Jing R L. Genetic dissection of developmental behavior of grain weight in wheat under diverse temperature and water regimes. Genetica, 2012, 140: 393-405.

[15]Moffat J M, Sears G, Cox T S, Paulsen G M. Wheat high temperature tolerance during reproductive growth: Ⅰ. Evaluation by chlorophyll fluorescence. Crop Science, 1990, 30: 881-885.

[16]邹琦. 植物生理生化实验指导. 北京: 中国农业出版社, 1995: 41-46.

Zou Q. Plant Physiology and Chemistry Laboratory Manual. Beijing: China Agriculture Press, 1995: 41-46. (in Chinese)

[17]Brucker P L, Frohberg R C. Stress tolerance and adaptation in spring wheat. Crop Science, 1987, 27: 3l-36.

[18]徐如强, 孙其信, 张树榛. 春小麦耐热性的筛选方法与指标. 华北农学报, 1997, 12(3): 22-29.

Xu R Q, Sun Q X, Zhang S Z. Screening methods and index for spring wheat heat resistance. Acta Agriculturae Boreali-Sinica, 1997, 12(3): 22-29. (in Chinese)

[19]周晓果, 景蕊莲, 郝转芳, 昌小平, 张正斌. 小麦幼苗根系性状的QTL分析. 中国农业科学, 2005, 38: 1951-1957.

Zhou X G, Jing R L, Hao Z F, Chang X P, Zhang Z B. Mapping QTL for seedling root traits in common wheat. Scientia Agricultura Sinica, 2005, 38: 1951-1957. (in Chinese)

[20]Hao Z F, Chang X P, Guo X J, Jing R L, Li R Z, Jia J A. QTL mapping for drought tolerance at stages of germination and seedling in wheat (Triticum aestivum L.) using a DH population. Agricultural Science in China, 2003, 2(9): 943-949.

[21]McIntosh R A, Hart G E, Devos K M, Rogers W J. Catalogue of gene symbols for wheat. http://grain.jouy.inra.fr/ggpages/wgc, 1999.

[22]陈希勇, 孙其信, 孙长征. 春小麦耐热性表现及其评价. 中国农业大学学报, 2000, 5(1): 43-49.

Chen X Y, Sun Q X, Sun C Z. Performance and evaluation of spring wheat heat tolerance. Journal of China Agricultural University, 2000, 5(1): 43-49. (in Chinese)

[23]Hays D B, Do J H, Mason R E, Morgan G, Finlayson S A. Heat stress induced ethylene production in developing wheat grains induces kernel abortion and increased maturation in a susceptible cultivar. Plant Science, 2007, 172: 1113-1123.

[24]李世平, 昌小平, 王成社, 景蕊莲. 小麦幼苗耐热性的QTL定位分析. 西北植物学报, 2012, 32(8): 1525-1533.

Li S P, Chang X P, Wang C S, Jing R L. Mapping QTLs for seedling traits and heat tolerance index in common wheat. Acta Botanica Boreali-Occidentalia Sinica, 2012, 32(8): 1525-1533. (in Chinese)

[25]邢永忠, 徐才华, 金平国. 水稻圆锥花序性状QTL与环境互作分析. 遗传学报, 2001, 28: 439-446.

Xing Y Z, Xu C H, Jin P G. Analysis of QTL×environment interaction for rice panicle characteristics. Acta Genetica Sinica, 2001, 28: 439-446. (in Chinese)

[26]Barakat M N, Al-Doss A A, Elshafei A A, Moustafa K A. Identification of new microsatellite marker linked to the grain filling rate as indicator for heat tolerance genes in F2 wheat population. Australian Journal of Crop Science, 2011, 5(2): 104-110.