中国农业科学 ›› 2013, Vol. 46 ›› Issue (6): 1201-1207.doi: 10.3864/j.issn.0578-1752.2013.06.013

• 园艺 • 上一篇    下一篇

葡萄芽自然休眠期间的呼吸代谢变化

 丛深, 王海波, 王孝娣, 王宝亮, 郑晓翠, 史祥宾, 刘万春, 刘凤之   

  1. 中国农业科学院果树研究所/农业部园艺作物种质资源利用重点实验室,辽宁兴城125100
  • 收稿日期:2012-12-11 出版日期:2013-03-15 发布日期:2013-01-17
  • 通讯作者: 通信作者刘凤之,Tel:13904295109;E-mail:liufengzhi6699@126.com
  • 作者简介:丛深,Tel:13699263118;E-mail:xyq058829 @sohu.com
  • 基金资助:

    国家现代农业产业技术体系建设专项(nycytx-30-zp)、中央级公益性科研院所基本科研业务费专项(1610032012006)

Respiratory Changes During Dormancy of Grape Buds

 CONG  Shen, WANG  Hai-Bo, WANG  Xiao-Di, WANG  Bao-Liang, ZHENG  Xiao-Cui, SHI  Xiang-Bin, LIU  Wan-Chun, LIU  Feng-Zhi   

  1. Fruit Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Germplasm Resources Utilization) , Ministry of Agriculture, Xingcheng 125100, Liaoning
  • Received:2012-12-11 Online:2013-03-15 Published:2013-01-17

摘要: 【目的】探讨葡萄芽呼吸代谢与自然休眠解除的关系,为葡萄人工休眠调控技术措施的制定提供理论依据。【方法】以高需冷量品种夏黑和低需冷量品种京蜜为试材,采取呼吸抑制剂法借助氧电极研究休眠解除过程中芽的呼吸代谢变化。【结果】不同需冷量品种休眠期间芽的呼吸速率和各呼吸途径所占比例大致相同,而且变化趋势也基本一致,但低需冷量品种京蜜呼吸速率及呼吸途径发生显著变化的时间比高需冷量品种夏黑提前20 d左右,且变化更为迅速,只是变化幅度略小于夏黑。休眠期间总呼吸速率呈单曲线变化,当休眠解除时达到顶峰。底物水平上,糖酵解-三羧酸途径所占比例在休眠解除时大幅度增加。电子传递链水平上,剩余呼吸和交替途径在休眠解除时显著增加。【结论】不同品种对低温的敏感度不同,低需冷量品种对低温刺激更为敏感。糖酵解-三羧酸途径是葡萄芽休眠解除的关键因素,剩余呼吸和交替途径的激活在葡萄芽休眠解除过程中起重要作用。

关键词: 葡萄 , 自然休眠 , 需冷量 , 呼吸代谢 , 呼吸途径

Abstract: 【Objective】The relationship between respiration and dormancy release of grape bud was discussed for the purpose of laying a theoretical foundation for the artificial control technology of dormancy. 【Method】Respiration inhibitors and oxygen electrode were used to investigate the respiratory changes during dormancy in grape buds (high chilling requirement grape V. vinifera-V.labrusca cv. Summer Black Seedless and low chilling requirement grape V. vinifera cv. Jingmi). 【Result】Different chilling requirement grape cultivars had the similar respiration rate, pathway rate and change tendency. Respiration rate and pathway of low chilling requirement grape Jingmi changed notably about 20 days before high chilling requirement grape Summer Black Seedless, and rapider. The change range of Jingmi was lower than Summer Black Seedless. The variation in total respiratory rate showed single peak curves. EMP (embden-meyerhof-parnas pathway)-TCA (tricarboxylic acid cycle) increased during dormancy release. On level of electron transport, alternative pathway and residual pathway were activated during dormancy release.【Conclusion】Different chilling requirement grape cultivars had different sensitivities, low chilling requirement grape cultivars were more sensitive to low temperature. EMP-TCA was the key of dormancy release. Alternative pathway and residual pathway might be an important role in dormancy release.

Key words: grape , dormancy , chilling requirement , respiratory , respiratory pathway