鹅源坦布苏病毒SHYG株的分离与鉴定

doi:10.3864/j.issn.0578-1752.2013.05.020

摘要/Abstract

摘要： 【目的】分离鹅源坦布苏病毒，并研究其对雏鹅的致病性。【方法】用RT-PCR方法对江苏省3个鹅场送检的产蛋期病鹅样品进行检测；对坦布苏病毒核酸阳性的样品进行病毒分离，测定分离病毒的生物学特性和E蛋白基因序列。【结果】这些样品中坦布苏病毒呈阳性；从病料中分离鉴定一株病毒，命名为SHYG。E蛋白基因序列同源性比对显示其与中国鸭源坦布苏病毒同源性达到99.3%；生物学特性研究表明该病毒可致Vero细胞病变，对小鼠无致病性，接种2周龄雏鹅出现精神沉郁、腹泻、神经症状甚至死亡；组织学变化可见肝脏淤血、胆小管扩张、脂肪变性；肺淤血、炎性渗出及含铁血黄素沉着，脾脏网状内皮细胞空泡化，肾出血，脑充血、胶质细胞增生。【结论】坦布苏病毒SHYG株对雏鹅有较强的致病性。

关键词: 鹅 , 坦布苏病毒 , 致病性

Abstract: 【Objective】 The objective of the study is to isolate Tembusu virus from goose and explore its pathogenicity to young goose.【Method】RT-PCR was applied to detect Tembusu virus in sick geese samples collected from three farms in Jiangsu, in which the positive samples were used to isolate Tembusu virus. The biological characteristics and sequence of Envelope (E) gene of isolated virus were determined.【Result】These samples were all PCR positive for Tembusu virus. One strain was isolated from a positive sample and named as SHYG. Sequence analysis based on E gene suggested that its sequence was homologous highly with that of Tembusu viruses isolated from ducks in China. The SHYG strain induced cytopathic effect in Vero cell. The results of animal experiment revealed that the SHYG strain caused depression, diarrhea, neural symptom, and even death when it was inoculated to 2-week-old geese, but no pathogenic to mice. The histological observation showed congestion, bile capillary expansion and steatosis in liver, hemorrhage, inflammatory exudate and hemosiderin pigmentation in lung, reticuloendothelial cell vacuolization in spleen, hemorrhage in kidney, and hyperemia and proliferation of gliocyte in brain. 【Conclusion】 The Tembusu virus strain SHYG is pathogenic to young goose.

Key words: goose , Tembusu virus , pathogenic

潘金金, 邹晓艳, 李鑫, 宿春虎, 柴茂, 姜逸, 胡艳芬, 赵国, 王彦红, 石火英, 陈素娟, 彭大新. 鹅源坦布苏病毒SHYG株的分离与鉴定[J]. 中国农业科学, 2013, 46(5): 1044-1053.

PAN Jin-Jin, ZOU Xiao-Yan, LI Xin, SU Chun-Hu, CHAI Mao, JIANG Yi, HU Yan-Fen, ZHAO Guo, WANG Yan-Hong, SHI Huo-Ying, CHEN Su-Juan, PENG Da-Xin. Isolation and Identification of Tembusu Virus Strain SHYG from Goose[J]. Scientia Agricultura Sinica, 2013, 46(5): 1044-1053.

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