中国农业科学 ›› 2013, Vol. 46 ›› Issue (2): 317-324.doi: 10.3864/j.issn.0578-1752.2013.02.011

• 园艺 • 上一篇    下一篇

地表覆膜对柑橘果实糖积累及蔗糖代谢酶活性的影响

 姜妮, 高海文, 金龙飞, 刘永忠, 彭抒昂   

  1. 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,武汉430070
  • 收稿日期:2012-07-19 出版日期:2013-01-15 发布日期:2012-10-16
  • 通讯作者: 通信作者刘永忠,Tel:027-87281897;E-mail:liuyongzhong@mail.hzau.edu.cn
  • 作者简介:姜妮,Tel:027-87281820;E-mail:balei1987@126.com
  • 基金资助:

    中央高校基本科研业务费专项资金资助 (2011PY102)、农业部国家现代农业(柑橘)产业体系专项资金(CARS-27)

The Impact of Plastic Film Mulching on Citrus Fruit Sugar Accumulation and Sucrose Metabolism-Related Enzymes

 JIANG  Ni, GAO  Hai-Wen, JIN  Long-Fei, LIU  Yong-Zhong, PENG  Shu-Ang   

  1. College of Horticulture and Forestry Sciences, Huazhong Agricultural University/Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan 430070
  • Received:2012-07-19 Online:2013-01-15 Published:2012-10-16

摘要: 【目的】研究地表覆膜(plastic film mulching,PFM)促进柑橘果实糖分积累增加的调控机制,丰富柑橘果实优质品质的形成理论知识。【方法】以鄂柑1号椪柑为材料,比较柑橘果实成熟期覆膜和对照之间叶片中可溶性总糖和淀粉含量、果实糖分积累以及蔗糖相关代谢酶活性的差异。【结果】与对照相比,覆膜处理使土壤水势降低,最终叶片中的可溶性总糖含量、果肉囊瓣皮和汁胞中可溶性糖含量显著增高;处理中囊瓣皮中的酸性转化酶和蔗糖合酶分解活性比对照显著增加,而汁胞中蔗糖合酶的分解活性降低,合成活性增加。【结论】覆膜使土壤产生了一定的水分胁迫,水分亏缺胁迫下诱发的渗透调节机制,使果实中糖分含量增加。其中蔗糖合酶是在覆膜情况下提高果肉库强,促进果实糖分积累增加的关键酶。

关键词: 椪柑 , 覆膜 , 糖积累 , 蔗糖代谢酶

Abstract: 【Objective】 The aim of this research is to investigate the possible physiological mechanism of the effect of plastic film mulching on citrus fruit sugar accumulation, which could help to enrich the knowledge of citrus high fruit quality formation. 【Method】 ‘Egan 1’ Ponkan (Citrus reticulata cv. Egan 1) was used as material, sugar accumulation and activities of sucrose metabolism-related enzymes were compared in fruit segment membrane and juice sacs, as well as contents of leaf total soluble sugar and starch between the plastic film mulching treatments and the control. 【Result】 Plastic film mulching at fruit ripening stage reduced soil water potential, which in the end increased the total soluble sugar content in leaf, fruit segment membrane and juice sacs. On the other hand, the activities of acid invertase and sucrose synthase (cleavage direction) in the segment membrane were higher, while in the juice sac the cleavage direction activity of sucrose synthase was lower in the plastic film mulching fruit than those in the control fruits. 【Conclusion】 Plastic film mulching caused water deficit stress, which induced osmotic adjustment and then to enhance sugar accumulation of fruits. Of them, the changes of sucrose synthase activities in segment membrane and juice sacs under plastic film mulching played an important role in increase of sink strength, thus increasing sucrose input and then enhancing sugar accumulation.

Key words: Ponkan , plastic mulch , sugar accumulation , sucrose metabolism-related enzymes