拟南芥TUA2参与ABA胁迫下的种子萌发过程

doi:10.3864/j.issn.0578-1752.2012.21.002

摘要/Abstract

摘要： 【目的】利用反向遗传学研究拟南芥TUA2与ABA途径相关基因的相互关系以及对种子萌发的影响。【方法】采用PCR、Tail PCR和RT-PCR技术对来自ABRC的T-DNA插入TUA2突变体进行插入位点的鉴定和转录活性的分析；利用转基因技术获得TUA2超表达植株；检测了含不同浓度ABA的MS培养基中各突变体和TUA2超表达植株的种子萌发；通过RT-PCR检测了突变体中ABA途径相关基因HAB、ABI1、RD22和P5CS的表达变化。【结果】突变体tua2-1和tua2-2的T-DNA插入位点均位于TUA2的启动子区，且二者的TUA2表达量均升高。在含有ABA（0.8 μmol•L-1）的MS培养基中，突变体和转基因等5个株系的种子萌发延迟，播种第5天萌发率在6%—18%，相同条件下的野生型种子萌发率为76%；ABI1和HAB在TUA2超表达体中的表达明显低于野生型，受ABA诱导后被诱导表达的程度也远低于野生型。【结论】TUA2可能参与了ABA信号途径对种子萌发过程的调节。

关键词: 拟南芥, 种子萌发, TUA2, ABA

Abstract: 【Objective】This study would characterize the TUA2 gene involving in seed germination under ABA stress in Arabidopsis by the method of reverse genetics. 【Method】 The Arabidopsis T-DNA inserted into TUA2 gene mutants were obtained from ABRC. The T-DNA insertion sites of mutants were analyzed by Tail PCR. Over-expression constructs of TUA2 were introduced into Arabidopsis by an Agrobacterium-mediated method. RT-PCR was used to detect TUA2 and ABA-responsive genes accumulation in mutants and transgenic plants. The seed germination of transgenic and mutant lines was tested on MS medium with different concentrations of ABA. 【Result】 The T-DNA was inserted into the gene promoter region in mutants tua2-1 and tua2-2, and led to the increasing accumulation of the TUA2. In the medium of MS/0.8 μmol•L-1 ABA, the seed germination rate of wild type was 76% after five days, whereas all the TUA2 over-expression lines, mutants and transgenic plants, were 6%-18%. The expression of ABII, HAB and P5CS was induced by ABA in wild type, but not in mutants and transgenics. 【Conclusion】 The T-DNA insertion of the mutants tua2-1 and tua2-2 resulted in the increasing expression of TUA2 gene. The TUA2 gene might be involved in plant responses to ABA by mediating the transcription of ABA-responsive genes.

Key words: Arabidopsis thaliana, seed germination, TUA2, ABA

刘海浩, 吴立柱, 岳智亮, 潘延云. 拟南芥TUA2参与ABA胁迫下的种子萌发过程[J]. 中国农业科学, 2012, 45(21): 4343-4350.

LIU Hai-Hao, WU Li-Zhu, YUE Zhi-Liang, PAN Yan-Yun. Arabidopsis TUA2 Gene Mediates Seed Germination Under ABA Stress[J]. Scientia Agricultura Sinica, 2012, 45(21): 4343-4350.

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