采后ABA处理促进‘陇薯3号’马铃薯块茎愈伤形成

doi:10.3864/j.issn.0578-1752.2017.20.014

摘要/Abstract

摘要： 【目的】研究外源ABA处理对采后‘陇薯3号’马铃薯块茎愈伤效果的影响，分析苯丙烷代谢及抗氧化酶在愈伤中的作用机理。【方法】以‘陇薯3号’马铃薯块茎为试材，人工损伤后用不同浓度脱落酸（ABA）处理，评价处理块茎在常温（（20±3）℃，湿度（80±5）%）及黑暗条件下的愈伤效果，分析100 mg·L^-1ABA处理后块茎伤口处组织苯丙烷代谢关键酶活性和代谢产物积累以及过氧化物酶和多酚氧化酶的活性变化，扫描电镜观察伤口处愈伤组织的形成过程.【结果】不同浓度ABA处理均有效促进了块茎愈伤，其中以100 mg·L^-1的处理效果最好。ABA处理能显著提高块茎伤口处组织苯丙氨酸解氨酶、肉桂酸羟化酶、4-香豆酰-辅酶A-连接酶和肉桂醇脱氢酶的活性，并能使总酚、类黄酮和木质素的含量增加，其中，苯丙氨酸解氨酶的活性呈现双峰型变化，在处理后的第3天和第9天分别高出同期对照253.07%和125.18%。此外，总酚和类黄酮含量在处理后第6天分别较同期对照高出48.60%和50.42%。ABA处理还明显增强了块茎伤口处组织中的过氧化物酶和多酚氧化酶活性。扫描电镜观察结果表明，ABA处理促进了块茎伤口表面愈伤封闭层和周皮的形成。【结论】外源ABA处理可通过促进马铃薯块茎伤口处组织的苯丙烷代谢，提高过氧化物酶和多酚氧化酶活性，加速马铃薯块茎愈伤周皮的形成。

关键词: 马铃薯块茎, 愈伤, ABA, 苯丙烷代谢, 抗氧化酶

Abstract: 【Objective】The experiment was conducted to study the effects of exogenous abscisic acid (ABA) treatment on wound healing of the potato tubers ‘Longshu No.3’, to analyze the role of phenylpropane metabolism and antioxidant enzymes in wound healing. 【Method】The potato cultivar ‘Longshu No.3’ was used as materials. The tubers were artificially damaged, then treated with different concentrations ABA, and healed under dark condition ((20±3)℃, RH (80±5)%). The healing ability of treated tubers was evaluated. The changes of activity of phenylpropane metabolism key enzymes, peroxidase and polyphenol oxidase were determined. The content of metabolites was determined at wounded site of tubers treated with 100mg·L^-1ABA during healing. The formation of healing tissue was observed by scanning electron microscopy. 【Result】ABA treatment at different concentrations effectively promoted wound healing of tubers, the screened optimum concentration was 100 mg·L^-1. ABA treatment at 100 mg·L^-1 significantly increased the activity of phenylalnine ammonialyase, cinnamic acid hydroxylase, 4-coumaroyl-coenzyme A-ligase and cinnamyl alcohol dehydrogenase, sped the accumulation of total phenols, flavonoids and lignin at wounded site of tubers. In which, the activity of phenylalanine ammonialyase showed bimodal change, the treatment was 253.07% and 125.18% higher than the control after 3 days and 9 days of healing. The content of total phenols and flavonoids of the treatment showed 48.60% and 50.42% higher than that of the control. Moreover, the activities of peroxidase and polyphenol oxidase were significantly enhanced at wounded site of tubers. Observation of scanning electron microscopy showed that the formation of closing layer and wound periderm of tubers were accelerated after ABA treatment. 【Conclusion】Exogenous ABA treatment accelerates the formation of wound periderm of potato tubers by promoting the phenylpropane metabolism, and increasing the activities of peroxidase and polyphenol oxidase.

Key words: potato tubers, wound healing, abscisic acid, phenylpropane metabolism, antioxidant enzyme

李雪，吴觉天，王毅，姜红，毕阳，司敏，张静荣，徐洁. 采后ABA处理促进‘陇薯3号’马铃薯块茎愈伤形成[J]. 中国农业科学, 2017, 50(20): 4003-4011.

LI Xue, WU JueTian, WANG Yi, JIANG Hong, BI Yang, SI Min, ZHANG JingRong, XU Jie. Postharvest ABA Treatment Promote Wound Healing of Potato ‘Longshu No.3’ Tubers[J]. Scientia Agricultura Sinica, 2017, 50(20): 4003-4011.

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参考文献

[1] Dastmalchi K, Kallash L, Wang I, Phan V C, Huang W, Serra O, Stark R E. Defensive armor of potato tubers: nonpolar metabolite profiling, antioxidant assessment, and solid- state NMR compositional analysis of suberin-enriched wound- healing tissues. Journal of Agricultural and Food Chemistry, 2015, 63(30): 6810-6822.

[2] Xue H L, Bi Y, Wei J M, Tang Y M, Zhao Y, Wang Y. Effect of cultivars, Fusarium strains and storage temperature on trichothecenes production in inoculated potato tubers. Food Chemistry, 2014, 151: 236-242.

[3] LULAI E C, NEUBAUER J D. Wound-induced suberization genes are differentially expressed, spatially and temporally, during closing layer and wound periderm formation. Postharvest Biology and Technology, 2014, 90: 24-33.

[4] SCHREIBER L, FRANKE R, HARMANN K. Wax and suberin development of native and wound periderm of potato (Solanum tuberosum L.) and its relation to peridermal transpirations. Planta, 2005, 220(4): 520-530.

[5] LULAI E C, FREEMAN T P. The importance of phellogen cells and their structural characteristics in susceptibility and resistance to excoriation in immature and mature potato tuber (Solanum tuberosum L.) periderm. Annals of Botany, 2001, 88(4): 555-561.

[6] LULAI E C. The canon of potato science: skin-set and wound- healing/suberization. Potato Research, 2007, 50(3/4): 387-390.

[7] ZHANG J H, JIA, W S, YANG, J C, ISMAIL A M. Role of ABA in integrating plant responses to drought and salt stresses. Field Crops Research, 2006, 97(1): 111-119.

[8] SUTTLE J C, LULAI E C, HUCKLE L L, NEUBAUER J D. Wounding of potato tubers induces increases in ABA biosynthesis and catabolism and alters expression of ABA metabolic genes. Journal of Plant Physiology, 2013, 170(6): 560-566.

[9] LULAI E C, SUTTLE J C, PEDERSON S M. Regulatory involvement of abscisic acid in potato tuber wound-healing. Journal of Experimental Botany, 2008, 59(6): 1175-1186.

[10] WU J Q, BALDWIN I T. New insights into plant responses to attack from insect herbivores. Genetics, 2010, 44: 1-24.

[11] LEIDE J, HILDEBRANDT U, HARTUNG W, RIEDERER M, VOGG G. Abscisic acid mediates the formation of a suberized stem scar tissue in tomato fruits. New Phytologist, 2012, 194(2): 402-415.

[12] SOLIDAY C L, DEAN B B, KOLATTUKUDY P E. Suberization: inhibition by washing and stimulation by abscisic acid in potato disks and tissue culture. Plant Physiology, 1978, 61(2): 170-174.

[13] COTTLE W, KOLATTUKUDY P E. Abscisic acid stimulation of suberization. Plant Physiology, 1982, 70(3): 775-780.

[14] KUMAR G N M, LULAI E C, SUTTLE J C, KNOWLES N R. Age-induced loss of wound-healing ability in potato tubers is partly regulated by ABA. Planta, 2010, 232(6): 1433-1445.

[15] TAO X Y, MAO L C, LI J Y, CHEN J Y, LU W J, HUANG S. Abscisic acid mediates wound-healing in harvested tomato fruit. Postharvest Biology and Technology, 2016, 118: 128-133.

[16] 陶晓亚, 李家寅, 韩雪源, 黄斯, 茅林春. 脱落酸对采后番茄愈伤次生代谢物质合成的影响. 食品工业科技, 2016(16): 330-334.

Tao X Y, Li J Y, Han X Y, Huang S, Mao L C. Effect of abscisic acid on secondary metabolite production during wound-healing in postharvest tomato fruit. Science and Technology of Food Industry, 2016(16): 330-334. (in Chinese)

[17] 姜红, 毕阳, 李昌健, 王毅, 李生娥, 刘耀娜, 王斌. 马铃薯品种‘青薯168’和‘陇薯3号’块茎愈伤能力的比较. 中国农业科学, 2017, 50(4): 774-782.

JIANG H, BI Y, Li C J, WANG Y, LI S E, LIU Y N, WANG B. A comparison of healing ability on potato tuber cultivars ‘Qingshu No. 168’ and ‘Longshu No. 3’. Scientia Agricultura Sinica, 2017, 50(4): 774-782. (in Chinese)

[18] 杨志敏, 毕阳, 李永才, 寇宗红, 包改红, 刘成琨, 王毅, 王蒂. 马铃薯干腐病菌侵染过程中切片组织细胞壁降解酶的变化. 中国农业科学, 2012, 45(1): 127-134.

YANG Z M, BI Y, LI Y C, KOU Z H, BAO G H, LIU C K, WANG Y, WANG D. Changes of cell wall degrading enzymes in potato tuber tissue slices infected by Fusarium sulphureum. Scientia Agricultura Sinica, 2012, 45(1): 127-134. (in Chinese)

[19] YIN Y, LI Y C, BI Y, CHEN S J, LI Y C, YUAN L, WANG Y, WANG D. Postharvest treatment with β-aminobutyric acid induces resistance against dry rot caused by Fusarium sulphureum in potato tuber. Agricultural Sciences in China, 2010, 9(9): 1372-1380.

[20] LAMB C J, RUBERY P H. A spectrophotometric assay for trans-cinnamic acid 4-hydroxylase activity. Analytical Biochemistry, 1975, 68(2): 554-561.

[21] SCHOCH G A, NIKOV G N, ALWORTH W L, Werck-Reichhart D. Chemical inactivation of the cinnamate 4-hydroxylase allows for the accumulation of salicylic acid in elicited cells. Plant Physiology, 2002, 130(2): 1022-1031.

[22] GOFFNER D, JOFFROY I, GRIMA P J, HALPIN C, KNIGHT M E, SCHUCH W A M. Purification and characterization of isoforms of cinnamyl alcohol dehydrogenase from Eucalyptus xylem. Planta, 1992, 188(1): 48-53.

[23] BAO G H, BI Y, LI Y C, KOU Z H, HU L G, GE Y H, WANG Y, WANG D. Overproduction of reactive oxygen species involved in the pathogenicity of Fusarium in potato tubers. Physiological and Molecular Plant Pathology, 2014, 86: 35-42.

[24] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 1976, 72(1/2): 248-254.

[25] 包改红, 毕阳, 李永才, 王毅, 王婷, 唐瑛, 马朝玲, 白小东. 硫色镰刀菌接种对抗病/易感品种马铃薯块茎苯丙烷代谢的影响比较. 食品科学, 2015, 36(6): 1002-6630.

BAO G H, BI Y, LI Y C, WANG Y, WANG T, TANG Y, MA C L, BAI X D. Comparison of phenylpropanoid pathway metabolism in slices of susceptible and resistant potato cultivars inoculated with Fusarium sulphureum. Food Science, 2015, 36(6): 1002-6630. (in Chinese)

[26] ALBA C M, DE FORCHETTI S M, TIGIER H A. Phenoloxidase of peach (Prunus persica) endocarp: its relationship with peroxidases and lignification. Physiologia Plantarum, 2000, 109(4): 382-387.

[27] YANG W L, BERNARDS M A. Metabolite profiling of potato (Solanum tuberosum L.) tubers during wound-induced suberization. Metabolomics, 2007, 3(2): 147-159.

[28] RIVERO R M, RUIZ J M, GARCA P C, LOPEZ-LEFEBRE L R, SANCHEZ E, ROMERO L. Resistance to cold and heat stress: accumulation of phenolic compounds in tomato and watermelon plants. Plant Science, 2001, 160(2): 315-321.

[29] CAMPOS R, NONOGAKI H, SUSLOW T, SALTVEIT M E. Isolation and characterization of a wound inducible phenylalanine ammonia-lyase gene (LsPAL1) from Romaine lettuce leaves. Physiologia Plantarum, 2004, 121(3): 429-438.

[30] SEWALT V, NI W, BLOUNT J W, JUNG H G, MASOUD S A, HOWLES P A, LAMB C, DIXON R A. Reduced lignin content and altered lignin composition in transgenic tobacco down-regulated in expression of L-phenylalanine ammonia-lyase or cinnamate 4- hydroxylase. Physiologia Plantarum, 1997, 115(1): 41-50.

[31] BENVENIST I, SALAUN J P, DURST F. Wounding-induced cinnamic acid hydroxylase in Jerusalem artichoke tuber. Phytochemistry, 1977, 16(1): 69-73.

[32] 赵淑娟, 刘涤, 胡之璧. 植物 4-香豆酸:辅酶A-连接酶. 植物生理学通讯, 2006, 42(3): 529-538.

ZHAO S J, LIU D, HU Z B. 4-Coumarate:coenzyme A ligase in plant. Plant Physiology Newsletter, 2006, 42(3): 529-538.(in Chinese)

[33] BOUDET A M. Lignins and lignification: selected issues. Plant Physiology and Biochemistry, 2000, 38(1): 81-96.

[34] KIM Y H, BAE J M, HUH G H. Transcriptional regulation of the cinnamyl alcohol dehydrogenase gene from sweet potato in response to plant developmental stage and environmental stress. Plant Cell Reports, 2010, 29(7): 779-791.

[35] WHETTEN R, SEDEROFF R. Lignin biosynthesis. The Plant Cell, 1995, 7(7): 1001-1013.

[36] 郭光艳, 柏峰, 刘伟, 秘彩莉. 转录因子对木质素生物合成调控的研究进展. 中国农业科学, 2015, 48(7): 1277-1287.

GUO G Y, BAI F, LIU W, BI C L. Advances in research of the regulation of transcription factors of lignin biosynthesis. Scientia Agricultura Sinica, 2015, 48(7): 1277-1287. (in Chinese)

[37] LIN J S, LIN C C, LIN H H, CHEN Y C, JENG S T. MicroR828 regulates lignin and H₂O₂ accumulation in sweet potato on wounding. New Phytologist, 2012, 196(2): 427-440.

[38] JIMEMEZ-AGUILAR D M, GRUSAK M A. Minerals, vitamin C, phenolics, flavonoids and antioxidant activity of Amaranthus leafy vegetables. Journal of Food Composition and Analysis, 2017, 58: 33-39.

[39] REYES L F, CISNEROS-ZEVALLOS L. Wounding stress increases the phenolic content and antioxidant capacity of purple-flesh potatoes (Solanum tuberosum L.). Journal of Agricultural and Food Chemistry, 2003, 51(18): 5296-5300.

[40] GOLUBENKO Z, AKHUNOV A, KHASHIMOVA N, BERESNEVA Y, MUSTAKIMOVA E, IBRAGIMOV F, ABDURASHIDOVA N, STIPANOVIC R. Induction of peroxidase as a disease resistance response in resistant (Hibiscus trionum) and susceptible (Althea armeniaca) species in the family Malvaceae. Phytoparasitica, 2007, 35(4): 401-413.

[41] YANG W L, BERNARDS M A. Wound-induced metabolism in potato (Solanum tuberosum) tubers. Plant Signaling and Behavior, 2006, 1(2): 59-66.

[42] ROBERTS E, KOLATTUKUDY P E. Molecular cloning, nucleotide sequence, and abscisic acid induction of a suberization-associated highly anionic peroxidase. Molecular and General Genetics MGG, 1989, 217(2/3): 223-232.

[43] 王云飞, 毕阳, 任亚琳, 王毅, 范存斐, 李大强, 杨志敏. 硅酸钠处理对厚皮甜瓜果实采后病害的控制及活性氧代谢的作用. 中国农业科学, 2012, 45(11): 2242-2248.

WANG Y F, BI Y, REN Y L, WANG Y, FAN C F, LI D Q, YANG Z M. Control of postharvest diseases and potentiation of reactive oxygen species metabolism in muskmelon (Cucumis melo L.) fruits treated by sodium silicate. Scientia Agricultura Sinica, 2012, 45(11): 2242-2248. (in Chinese)

[44] 谢春艳, 宾金华, 陈兆平, 莫熙穆. 多酚氧化酶及其生理功能. 生物学通报, 1999, 34(6): 11-14.

XIE C Y, BIN J H, CHEN Z P, MO X M. Polyphenol oxidase and its physiological function. Biology Bulletin, 1999, 34(6): 11-14. (in Chinese)

[45] THIPYAPONG P, HUNT M D, STEFFENS J C. Systemic wound induction of potato (Solanum tuberosum) polyphenol oxidase. Phytochemistry, 1995, 40(3): 673-676.

[46] SONG W W, MA X R, TAN H, ZHOU J Y. Abscisic acid enhances resistance to Alternaria solani in tomato seedlings. Plant Physiology and Biochemistry, 2011, 49(7): 693-700.

[47] SABBA R P, LULAI E C. Histological analysis of the maturation of native and wound periderm in potato (Solanum tuberosum L.) tuber. Annals of Botany, 2002, 90(1): 1-10.

[48] SABBA R P, LULAI E C. Immunocytological comparison of native and wound periderm maturation in potato tuber. American Potato Journal, 2004, 81(2): 119-124.