不同水平维生素A对意大利蜜蜂春繁阶段群势及幼虫抗氧化性的影响

doi:10.3864/j.issn.0578-1752.2012.17.015

摘要/Abstract

摘要： 【目的】研究日粮中添加不同水平维生素A对意大利蜜蜂春繁阶段群势和幼虫虫体抗氧化活性的影响。【方法】春繁开始时选用本地意大利蜜蜂25群（群内无花粉贮备），随机分为5组，分别饲喂添加了不同水平维生素A的试验日粮，维生素A水平分别为0、5 000、10 000、15 000、20 000 IU•kg-1，并测定各组蜂群群势及幼虫抗氧化活性。【结果】春繁阶段饲粮中维生素A添加水平对意大利蜜蜂群势及取食量有显著影响。在试验期末，维生素A水平为10 000 IU•kg-1组群势显著高于对照组和其它试验组（P＜0.05）；饲喂维生素A水平为10 000和15 000 IU•kg-1饲料时5日龄幼虫虫体抗氧化活性（总抗氧化能力（T-AOC）、总超氧化物岐化酶（T-SOD）活性、过氧化氢酶（CAT）活性）显著高于对照组和其它试验组（P＜0.05），丙二醛（MDA）含量显著低于对照组和其它试验组（P＜0.05）；除维生素A水平为15 000 IU•kg-1组外，10 000 IU•kg-1组工蜂初生重显著高于对照组和其它试验组（P＜0.05）。【结论】意大利蜜蜂春繁阶段适宜的维生素A添加水平（10 000—15 000 IU•kg-1）有利于蜂群群势的增长和抗氧化能力的提高。

关键词: 蜜蜂, 维生素A, 营养需求, 群势, 抗氧化性

Abstract: 【Objective】 The objective of this study is to study the effect of different levels of vitamin A on the population and larvae antioxidation of A. mellifera ligustica during the period of spring multiplication. 【Method】 Twenty-five colonies of honeybees (no pollen in colonies) from a local apiary were used in the study. The honeybee colonies were randomly allocated into 5 groups with 5 replicaties for each. The 5 groups of honeybees were fed with basal diet with supplementation of vitamin A at the levels of 0 (group 1), 5 000 (group 2), 10 000 (group 3), 15 000 (group 4) and 20000 IU•kg-1 (group 5), respectively. The colony development and antioxidant activities of larvae of the 5 groups were evaluated. 【Result】 During the period of spring multiplication, there were significant differences in colony development and feeding amounts of the groups fed with different levels of vitamin A. The colony development of group 3 was significantly faster than the control group and other experimental groups (P＜0.05) at the end of trial. The antioxidant activities (T-SOD, CAT and T-AOC) of 5-day-old larvae of group 3 and group 4 were significantly higher than the control and other experimental groups (P＜0.05). The content of MDA was significantly lower than the control and other experimental groups (P＜0.05). Except for group 4, the workers’ emergent weight of group 3 was significantly higher than the control and other experimental groups (P＜0.05).【Conclusion】Optimal diet vitamin A supplementation (10 000-15 000 IU•kg-1) will increase the population of bee colony and antioxidant activities of larvae of A. mellifera ligustica during the period of spring multiplication.

Key words: honeybee, vitamin A, nutrient requirements, bee population, antioxidant activities

冯倩倩, 杨维仁, 胥保华, 李成成. 不同水平维生素A对意大利蜜蜂春繁阶段群势及幼虫抗氧化性的影响[J]. 中国农业科学, 2012, 45(17): 3584-3591.

FENG Qian-Qian, YANG Wei-Ren, XU Bao-Hua, LI Cheng-Cheng. Effect of Different Levels of Vitamin A on the Colony Development and Larvae Antioxidation of Apis mellifera ligustica During he Period of Spring Multiplication[J]. Scientia Agricultura Sinica, 2012, 45(17): 3584-3591.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2012.17.015

https://www.chinaagrisci.com/CN/Y2012/V45/I17/3584

参考文献

[1]Campos M G R, Bogdanov S, de Almeida-MuradianL B, Szczesna T, Mancebo Y, Frigerio C, Ferreira F. Pollen composition and standardisation of analytical methods. Journal of Apicultural Research and Bee World, 2008, 47(2): 156-163.

[2]王开发, 张玉兰. 花粉中维生素的研究. 蜜蜂杂志, 1997(2): 3-6.

Wang K F, Zhang Y L. Study on vitamins in pollen. Journal of Bee, 1997(2): 3-6. (in Chinese)

[3]赵宗礼. 养蜂技术指导. 北京: 金盾出版社, 1988: 14.

Zhao Z L. Beekeeping Technical Director. Beijing: Jindun Publishing House, 1988: 14. (in Chinese)

[4]Palacios A, Piergiacomi V A, CatalaA. Vitamin A supplementation inhibits chemiluminescence and lipid perorxidation in isolated rat liver microsomes and mitochondria. Molecular and Cellular Biochemistry, 1996, 154: 77-82.

[5]李英哲, 黄连珍, 周丽玲. 维生素A缺乏对大鼠脂质过氧化和抗氧化系统的影响. 营养学报, 2001, 23(1): 1-4.

Li Y Z, Huang L Z, Zhou L L. Effect of vitamin A deficiency on lipid peroxidation and antioxidation system in rats. Acta Nutriment Sinica, 2001, 23(1): 1-4. (in Chinese)

[6]Herbert Jr E W, Shimanuki H. Effect of fat soluble vitamins on the brood rearing capabilities of honey bees fed a synthetic diet. Annals of the Entomological Society of America, 1978, 71(5): 689-691.

[7]冯倩倩, 胥保华, 李成成, 杨维仁. 维生素A 对意大利蜜蜂群势、封盖子量及抗氧化性的影响. 动物营养学报, 2011, 23(6): 971-975.

Feng Q Q, Xu B H, Li C C, Yang W R. Effects of vitamin A on bee population, sealed brood quantity and antioxidant of Apis mellifera ligustica. Chinese Journal of Animal Nutrition, 2011, 23(6): 971-975. (in Chinese)

[8]王颖, 马兰婷, 胥保华. 蜜蜂营养需要研究的必要性及策略. 动物营养学报, 2011, 23(8): 1269-1272.

Wang Y, Ma L T, Xu B H. Necessity and strategy for studying the nutrient requirement of honeybee. Chinese Journal of Animal Nutrition, 2011, 23(8): 1269-1272. (in Chinese)

[9]李成成, 胥保华, 冯倩倩, 杨维仁. 蜜蜂的营养需要及其研究方法. 中国蜂业, 2010, 61(2): 14-15.

Li C C, Xu B H, Feng Q Q, Yang W R. The nutrition and research methods of honeybee. Apiculture of China, 2010, 61(2): 14-15. (in Chinese)

[10]李杰銮, 王志, 丁艳波. 不同方式饲喂花粉对蜂群春繁的影响. 养蜂科技, 2005(3): 2-3.

Li J L, Wang Z, Ding Y B. Effect of pollen fed in different way on breeding of colony in spring. Apicultural Science and Technology, 2005(3): 2-3. (in Chinese)

[11]DeGrandi-Hoffman G, Wardell G, Ahumada-Segura F, Rinderer T, Danka R, Pettis J. Comparisons of pollen substitute diets for honey bees: consumption rates by colonies and effects on brood and adult populations. Journal of Apicultural Research, 2008, 47(4): 265-270.

[12]Bogdanov S. Royal jelly, bee brood: composition, health, medicine: a review. Bee Product Science, 2011(9): 1-31.

[13]Somerville D. Fat Bees Skinny Bees - A Manual on Honey Bee Nutrition for Beekeepers. Goulburn: Rural Industries Research and Development Corporation, 2005: 1-138.

[14]Mackay W J, Bewley G C. The genetics of catalase in Drosophila melanogaster: isolation and characterization of acatalasemic mutants. Genetics, 1989, 122(3): 643-652.

[15]Parkes T L, Hilliker A J, Phillips J P. Genetic and biochemical analysis of glutathione-S-transferase in the oxygen defense system of Drosophila melanogaster. Genome, 1993, 36(6): 1007-1014.

[16]任国峰, 黄忆明, 刘富强. 维生素A水平对孕期大鼠脂质过氧化及抗氧化系统的影响. 实用预防医学, 2001, 8(3): 173-175.

Ren G F, Huang Y M, Liu F Q. Influence of vitamin A level on lipid peroxidation and antioxidation system in pregnant rats. Practical Preventive Medicine, 2001, 8(3): 173-175. (in Chinese)

[17]韩磊, 马爱国, 张燕. 维生素A干预对大鼠抗氧化能力及细胞膜流动性影响的研究. 卫生研究, 2004, 33(4): 450-452.

Han L, Ma A G, Zhang Y. Effect of different dosage of vitamin A supplement on anti-oxidative activity and the fluidity of erythrocyte membrane in rats. Journal of Hygiene Research, 2004, 33(4): 450-452. (in Chinese)

[18]徐伟, 程彬, 袁海滨, 史树森. 黄褐油葫芦不同发育阶段维生素含量与抗氧化酶活性分析. 东北林业大学学报, 2005, 33(5): 86-88.

Xu W, Cheng B, Yuan H B, Shi S S. Analysis on the content of vitamin and activity of antioxidant enzyme of Teleoqzyllus derelictus Gorochov in different growth stage. Journal of Northeast Forestry University, 2005, 33(5): 86-88. (in Chinese)

[19]Chapman R F. The Insects: Structure and Function. UK: Cambridge University Press, 1998: 770.

[20]王志, 李杰銮, 丁艳波, 韦召凯, 王欢. 不同营养对蜜蜂初生重的影响. 吉林畜牧兽医, 2005(9): 5-7.

Wang Z, Li J L, Ding Y B, Wei Z K, Wang H. Effect of different nutriment on the weight of bees. Jilin Animal Husbandry and Veterinary Medicine, 2005(9): 5-7. (in Chinese)

[21]刘井兰，于建飞, 吴进才, 印建莉, 吴东浩. 昆虫活性氧代谢. 昆虫知识, 2006, 43(6): 752-756.

Liu J L, Yu J F, Wu J C, Yin J L, Wu D H. Insect reactive oxygen metabolization. Chinese Bulletin of Entomology, 2006, 43(6): 752-756. (in Chinese)

[22]Rackow E C, Astiz M E, Weil M H. Cellular oxygen metabolism during sepsis and shock: the relationship of oxygen consumption to oxygen delivery. Journal of American Medical Association, 1988, 259(13): 1989-1994.

[23]程艳华, 刘亚男, 胡福良, 郑火青, 金水华. 蛋白质营养水平对工蜂初生重和咽下腺发育的影响. 中国蜂业, 2008, 59(12): 11-13.

Cheng Y H, Liu Y N, Hu F L, Zheng H Q, Jin S H. Effect of protein nutritional levels on emergent weight and hypopharyngeal gland development of worker bee. Apiculture of China, 2008, 59(12): 11-13. (in Chinese)

[24]Nation J L. Insect Physiology and Biochemistry. Boca Raton, Fla.: CRC Press/Taylor & Francis, 2001: 485.

[25]Elaidy W K, Ebeid A A, Fathy H M, Salama S Z A. Effect of some food additives on workers and queens of honey bee (Apis mellifera L.). Journal of Plant Protection and Pathology, 2010, 1(12): 1013-1021.

[26]郭晓宇. 维生素A过量对肉鸡钙、磷沉积及碱性磷酸酶和骨钙素表达的影响[D]. 呼和浩特: 内蒙古农业大学, 2010.

Guo X Y. Effects of excessive vitamin A on deposition of calcium and phosphorus and expression of alkaline phosphatase and bone gla-protein in broiler chickens[D]. Huhhot: Inner Mongolia Agricultural University, 2010. (in Chinese)