中国农业科学 ›› 2012, Vol. 45 ›› Issue (13): 2568-2579.doi: 10.3864/j.issn.0578-1752.2012.13.002

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

不同环境下大豆荚粒性状的遗传与QTL分析

 梁慧珍, 余永亮, 杨红旗, 张海洋, 董薇, 李彩云, 巩鹏涛, 刘学义, 方宣钧   

  1. 1.河南省农业科学院芝麻研究中心,郑州 450002
    2.海南省热带农业资源开发利用研究所,海南三亚 572025
    3.山西省农业科学院经济作物研究所,山西汾阳 032200
  • 收稿日期:2012-01-30 出版日期:2012-07-01 发布日期:2012-04-05
  • 通讯作者: 梁慧珍,E-mail:lhzh66666@163.com
  • 作者简介:梁慧珍,E-mail:lhzh66666@163.com
  • 基金资助:

    河南省科技创新杰出人才计划(114200510002)、转基因生物新品种培育科技重大专项(2011ZX08004-005)、“948”国际引进项目(2011-G1-13)

Genetic Analysis and QTL Mapping of Pod-Seed Traits in Soybean Under Different Environments

 LIANG  Hui-Zhen, YU  Yong-Liang, YANG  Hong-Qi, ZHANG  Hai-Yang, DONG  Wei, LI  Cai-Yun, GONG  Peng-Tao, LIU  Xue-Yi, FANG  Xuan-Jun   

  1. 1.河南省农业科学院芝麻研究中心,郑州 450002
    2.海南省热带农业资源开发利用研究所,海南三亚 572025
    3.山西省农业科学院经济作物研究所,山西汾阳 032200
  • Received:2012-01-30 Online:2012-07-01 Published:2012-04-05

摘要: 【目的】探讨大豆荚粒性状之间以及与产量之间的相互关系及其遗传机制,并定位控制其性状的QTL。【方法】以栽培大豆晋豆23为母本,半野生大豆灰布支黑豆为父本所衍生的474个重组自交系,通过3年2个重复的试验结果,用多年多点联合分析方法对荚粒及产量等9个性状进行遗传分析及数量性状定位。【结果】相关分析结果表明,产量与百粒重、粒长、单株粒重、2粒荚、3粒荚呈现显著或极显著正相关,在三年两地共定位了6个产量QTL、4个百粒重QTL、10个单株粒重QTL、2个粒宽QTL、5个粒长QTL、7个1粒荚QTL、5个2粒荚QTL、7个3粒荚QTL和5个4粒荚QTL。【结论】在不同年份和环境下检测到的QTL,可作为荚粒性状改良的候选染色体区段,用于分子标记辅助选择,同时也要注意环境的影响。

关键词: 大豆, 荚粒性状, 遗传, QTL

Abstract: 【Objective】 Soybean [Glycine mar (L.) Merr.] pod-seed trait is one of the most important traits related with yields. In this paper, the relationship between soybean pod-seed traits and yields was studied, the QTLs controlling soybean pod-seed traits were mapped, and the soybean pod-seed trait molecular genetic mechanism was revealed. 【Method】Based on three years and two environments data, a recombinant line population of 474 F2:8:11 lines from a cross of Jindou 23 and ZDD2315 was used for analyzing and mapping QTLs of soybean pod-seed related traits. 【Result】The results of the molecular genetic analysis indicated that the field traits have a significant positive correlation or extreme significant positive correlation with 100-grain-weight, grain length, single grain weight, two-grain pod and three-grain pod. According to three years and two environments data, the authors mapped six QTLs of field traits related, four QTLs of 100-grain-weight, ten QTLs of single line grain weight, two QTLs of grain width, five QTLs of grain length, seven QTLs of one-grain pod, five two-grain pod, seven QTLs of three-grain pod and five QTLs of four-grain pod. 【Conclusion】The identification of consistent QTLs under different environments and in different years will be used as candidate chromosome regions, which will help to improve the efficiency of selection for pod-seed related traits in soybean through molecular marker-assisted selection.

Key words: soybean, pod-seed traits, genetic, QTL