中国荷斯坦奶牛IL-8基因SNP-180A/G显著影响体细胞评分

doi:10.3864/j.issn.0578-1752.2012.07.017

摘要/Abstract

摘要： 【目的】通过研究IL-8基因5′侧翼区多态性与体细胞评分（somatic cell score，SCS）的相关性，获得与低体细胞计数（somatic cell counts，SCC）性状相关的遗传标记，为低SCC性状的选育提供借鉴。【方法】采用PCR-SSCP技术检测来自30个公牛家系的610头中国荷斯坦奶牛的IL-8基因5′侧翼区的多态性，并采用混合动物模型分析多态位点与主要泌乳性状及SCS的相关性。【结果】IL-8基因5′侧翼区存在1个SNP位点，即-180 A/G；定义了3种基因型，即GG、GA和AA，频率分别为0.41、0.43和0.16。该SNP位点对SCS具有显著效应，对SCS指标来说，GG极显著低于GA或AA（P＜0.01），而该SNP位点对测定日产奶量、测定日乳脂率、测定日乳蛋白率和305 d校正产奶量的影响不显著（P＞0.05）。【结论】IL-8基因5′侧翼区SNP-180 A/G显著影响体细胞评分。

关键词: 中国荷斯坦奶牛, IL-8基因, SCS, SNP, 泌乳性状

Abstract: 【Objective】 Bovine interleukin-8 (IL-8) is one of the major factors recruiting neutrophils during cow mastitis and causing rapid increase of somatic cell counts (SCC). Knowledge of IL-8 polymorphisms would be helpful to genetic selection of low SCC. 【Method】 The SNPs in the 5′ upstream of bovine IL-8 gene were investigated in 610 Chinese Holstein cows from 30 bull families in a dairy farm in Shanghai using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique, and milking traits and somatic cell score (SCS) were measured and their relationship was analyzed using a mixed animal model. 【Result】 The results showed that one SNP, A(-180)G, was detected, determining three genotypes, GG, GA and AA with frequencies of 0.41, 0.43 and 0.16, respectively. The SNP showed significant effect on SCS, with GG having a significantly lower SCS than GA or AA (P＜0.01), while this SNP had no effect on test-day milk yield, test-day milk fat content, test-day milk protein content, and 305 d corrected milk yield. 【Conclusion】The findings demonstrated that the SNP -180A/G exerted significant effect on SCS.

Key words: Chinese Holstein cow, IL-8 gene, SCS, SNP, milking traits

冀德君, 陈仁金, 杨章平, 朱国强, 毛永江, 陈莹, 李锐. 中国荷斯坦奶牛IL-8基因SNP-180A/G显著影响体细胞评分[J]. 中国农业科学, 2012, 45(7): 1387-1391.

JI De-Jun, CHEN Ren-Jin, YANG Zhang-Ping, ZHU Guo-Qiang, MAO Yong-Jiang, CHEN Ying, LI Rui. IL-8 Gene SNP-180A/G Affects Significantly Somatic Cell Score in Chinese Holstein Cow[J]. Scientia Agricultura Sinica, 2012, 45(7): 1387-1391.

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