动物转基因新技术研究进展

doi:10.3864/j.issn.0578-1752.2011.10.023

摘要/Abstract

摘要： 动物转基因技术是将外源基因导入动物体内，外源基因随机整合或定点整合（打靶）在染色体基因组上并得到表达和遗传的生物技术。该技术自发现以来,在畜牧业、医药产业、环境保护以及新型生物材料等领域已得到了广泛应用。本文主要就近年来迅速发展起来的慢病毒载体导入法、精原干细胞法、锌指核酸酶介导的基因打靶、RNA干扰介导的基因沉默等新的高效转基因技术进行了概述。

关键词: 转基因技术, 动物, 慢病毒载体, 精原干细胞, 锌指核酸酶, RNA干扰

Abstract: Animal transgenic technology refers to the transfer of an exogenous gene into animal genome by random or site-specific integration and the foreign gene can be expressed and inherited to the offspring. Various technologies in animal transgenesis have now been widely applied to areas such as animal husbandry, medical industry, environmental protection and new biological materials production. This article reviews the recently developed technologies based on lentivectors, spermatogonial stem cells, zinc finger nucleases and RNA interference.

Key words: transgenic technology, animal, lentivectors, spermatogonial stem cells, zinc-finger nucleases, RNA interference

陈青, 曹文广. 动物转基因新技术研究进展[J]. 中国农业科学, 2011, 44(10): 2168-2175.

CHEN Qing, CAO Wen-Guang. Progress in Research of Animal Transgenic Technology[J]. Scientia Agricultura Sinica, 2011, 44(10): 2168-2175.

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